scholarly journals IDENTIFICATION AND CONTENT OF PHENOLIC COMPOUNDS IN A BUTANOL FRACTION OF EXTRACT FROM THE FLOWERS OF TANACETUM VULGARE

2021 ◽  
pp. 129-137
Author(s):  
Tat'yana Anatol'yevna Krol ◽  
Lidiya Viktorovna Zinnatshina ◽  
Dmitriy Nikolayevich Baleev ◽  
Николай Иванович Sidelnikov ◽  
Vladimir Ionovich Ossipov
Keyword(s):  
Phenolic Compounds ◽  
Pharmacological Activity ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Aromatic Plants ◽  
Medicinal And Aromatic Plants ◽  
Tanacetum Vulgare ◽  
Butanol Fraction ◽  
Array Detection ◽  
First Time

Tanacetum vulgare L. (syn.: Chrysanthemum vulgare (L). Bernh) is a perennial medicinal plant with a Euro-Asian type of habitat. Tansy extracts have a wide biological and pharmacological activity. At the All-Russian Institute of Medicinal and Aromatic Plants, on the basis of the butanol fraction of the T. vulgare flowers extract was developed a drug with high choleretic and anti-inflammatory activity. In this regard, the aim of this work was to study the qualitative composition and quantitative content of phenolic compounds of the butanol fraction, which are supposed to determine its biological activity. For this was obtained a phenolic compounds fraction, isolated from the extract of inflorescences of T. vulgare plants grown on the VILAR experimental plot. High-performance liquid chromatography with diode-array detection in combination with high-resolution mass spectrometry (UPLC-PDA-HRMS) was used for the analysis. Our studies have shown that the butanol fraction of T. vulgare contains 7 phenolic compounds, among which myricetin-3-glucoside was detected for the first time. The content of phenolic compounds reaches 90%, while the main compounds are neochlorogenic, 3,5- and 4,5-dicofeylquinic acids, the proportion of which is 69%. Thus, the obtained data indicate that the pharmacological activity of the drug based on the butanol fraction of the extract from tansy flowers is due to the high content of caffeoylquinic acids.

COMPOSITION AND CONTENT OF PHENOLIC COMPOUNDS IN DIFFERENT FRACTIONS OF EXTRACT FROM PLANTS OF ARNICA FOLIOSA NUTT.

2020 ◽  
pp. 139-147
Author(s):  
Tatiana Anatol'yevna Krol' ◽  
Lidiya Viktorovna Zinnatshina ◽  
Eugenia Ramil'yevna Gatiatulina ◽  
Andrey Ivanovich Radimich ◽  
Olga Leonidovna Saybel ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Total Content ◽  
Biologically Active ◽  
Diethyl Ester ◽  
Alternative Source ◽  
Caffeoylquinic Acid ◽  
Array Detection ◽  
Derivatives Of

Among representatives of the genus Arnica L., the Arnica montana L. species is the most studied and widely used for medical purposes. However, due to the fact that the A. montana species is endangered in most European countries, the possibility to use Arnica foliosa Nutt. as an alternative source of phytochemicals is being investigated. A deeper study of the chemical composition of A. foliosa extract may give the opportunity to expand the spectrum of its possible application. The purpose of the research was to perform a detailed study of the composition and content of biologically active compounds the aerial part of A. foliosa by high performance liquid chromatography with diode array detection in combination with high-resolution mass spectrometry. Sixteen phenolic compounds were detected in ethylacetate and butanol fractions of A. foliosa. There were identified thirteen derivatives of caffeoylquinic acid and three flavonoids. In the chloroform and diethyl ester fractions, phenolic compounds were absent. It was established that the ethylacetate fraction contains big amounts of two phenolic compounds – 3,4-dicaffeoylquinic and 4,5-dicaffeoylquinic acids, and eight compounds were presented in trace or very small quantities. All sixteen phenolic compounds were found in the butanol fraction, but their total content was almost 2-fold less than in the ethylacetate fraction.

scholarly journals Influence of Ligand Functionalization of UiO-66-Based Metal-Organic Frameworks When Used as Sorbents in Dispersive Solid-Phase Analytical Microextraction for Different Aqueous Organic Pollutants

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2869 ◽  
Author(s):  
Iván Taima-Mancera ◽  
Priscilla Rocío-Bautista ◽  
Jorge Pasán ◽  
Juan Ayala ◽  
Catalina Ruiz-Pérez ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Metal Organic Frameworks ◽  
Aqueous Sample ◽  
Optimization Study ◽  
Relative Standard ◽  
Polycyclic Aromatic ◽  
Metal Organic ◽  
Array Detection ◽  
First Time

Four metal-organic frameworks (MOFs), specifically UiO-66, UiO-66-NH2, UiO-66-NO2, and MIL-53(Al), were synthesized, characterized, and used as sorbents in a dispersive micro-solid phase extraction (D-µSPE) method for the determination of nine pollutants of different nature, including drugs, phenols, polycyclic aromatic hydrocarbons, and personal care products in environmental waters. The D-µSPE method, using these MOFs as sorbents and in combination with high-performance liquid chromatography (HPLC) and diode-array detection (DAD), was optimized. The optimization study pointed out to UiO-66-NO2 as the best MOF to use in the multi-component determination. Furthermore, the utilization of isoreticular MOFs based on UiO-66 with the same topology but different functional groups, and MIL-53(Al) to compare with, allowed us for the first time to evaluate the influence of such functionalization of the ligand with regards to the efficiency of the D-µSPE-HPLC-DAD method. Optimum conditions included: 20 mg of UiO-66-NO2 MOF in 20 mL of the aqueous sample, 3 min of agitation by vortex and 5 min of centrifugation, followed by the use of only 500 µL of acetonitrile as desorption solvent (once the MOF containing analytes was separated), 5 min of vortex and 5 min of centrifugation. The validation of the D-µSPE-HPLC-DAD method showed limits of detection down to 1.5 ng·L−1, average relative recoveries of 107% for a spiked level of 1.50 µg·L−1, and inter-day precision values with relative standard deviations lower than 14%, for the group of pollutants considered.

scholarly journals Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 783 ◽  
Author(s):  
Mirjana Mosić ◽  
Jelena Trifković ◽  
Irena Vovk ◽  
Uroš Gašić ◽  
Živoslav Tešić ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Ion Trap ◽  
Therapeutic Potential ◽  
Flavonoid Glycosides ◽  
Phenolic Composition ◽  
Bee Pollen ◽  
Orbitrap Mass Spectrometry ◽  
Health Promoting ◽  
First Time

Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.

scholarly journals Patterns of Variation and Chemosystematic Significance of Phenolic Compounds in the Genus Cyclopia (Fabaceae, Podalyrieae)

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2352
Author(s):  
Maria. A. Stander ◽  
Herman Redelinghuys ◽  
Keabetswe Masike ◽  
Helen Long ◽  
Ben-Erik Van Wyk
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
High Resolution Mass Spectrometry ◽  
Species Level ◽  
Large Dataset ◽  
Plant Parts ◽  
Negative Mode ◽  
First Time ◽  
Resolution Mass

As a contribution towards a better understanding of phenolic variation in the genus Cyclopia (honeybush tea), a collection of 82 samples from 15 of the 23 known species was analysed using liquid-chromatography–high resolution mass spectrometry (UPLC-HRMS) in electrospray ionization (ESI) negative mode. Mangiferin and isomangiferin were found to be the main compounds detected in most samples, with the exception of C. bowiena and C. buxifolia where none of these compounds were detected. These xanthones were found to be absent from the seeds and also illustrated consistent differences between species and provenances. Results for contemporary samples agreed closely with those based on analysis of a collection of ca. 30-year-old samples. The use of multivariate tools allowed for graphical visualizations of the patterns of variation as well as the levels of the main phenolic compounds. Exclusion of mangiferin and citric acid from the data was found to give better visual separation between species. The use of UPLC-HRMS generated a large dataset that allowed for comparisons between species, provenances and plant parts (leaves, pods, flowers and seeds). Phenetic analyses resulted in groupings of samples that were partly congruent with species but not with morphological groupings within the genus. Although different provenances of the same species were sometimes found to be very variable, Principle Component Analysis (PCA) indicated that a combination of compounds have some (albeit limited) potential as diagnostic characters at species level. 74 Phenolic compounds are presented, many of which were identified for the first time in Cyclopia species, with nine of these being responsible for the separation between samples in the PCAs.

Inhibition of metalloproteinase and proteasome activities in colon cancer cells by citrus peel extracts

2015 ◽  
Vol 26 (5) ◽  
Author(s):  
Ayokunle O. Ademosun ◽  
Ganiyu Oboh ◽  
Sabina Passamonti ◽  
Federica Tramer ◽  
Lovro Ziberna ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Phenolic Compounds ◽  
High Performance ◽  
Orange Peel ◽  
Proteasome Inhibition ◽  
Citrus Peel ◽  
Colon Cancer Cell Lines ◽  
Lovo Cells ◽  
Citrus Peels ◽  
Array Detection

AbstractCitrus peels are consumed in the form of infusions, candy or wine, based on their well-documented nutritional and medicinal properties. This study sought to investigate the effect of some citrus peels’ [grapefruit (The inhibition of MMP and proteasome activities in the cells by the peel extracts, as well as the identification of phenolic compounds using high-performance liquid chromatography with diode-array detection (HPLC-DAD), was determined.Orange peel extracts had the strongest inhibition of MMP in Caco-2 and LoVo cells, while shaddock had the least. Shaddock peel extracts also had the least MMP inhibition in LoVo/ADR lysates. Grapefruit had the least proteasome inhibition in Caco-2 and LoVo lysates, while there was no significant (p>0.05) difference in the proteasome inhibition of the peel extracts in LoVo/ADR lysates. The extracts inhibited proteasome activity in extract-treated cells, and HPLC fingerprinting of the extracts revealed the presence of some phenolic compounds such as quercetin, caffeic acid, kaempferol, catechin and naringin.The inhibition of MMP and proteasome activities in colon cancer cell lines suggests the potential use of citrus peels as functional food in the management and/or prevention of colon cancer.

Characterization of phenolic compounds from inner bark of Betula pendula

Holzforschung ◽  
2012 ◽  
Vol 66 (2) ◽  
Author(s):  
Jaana Liimatainen ◽  
Maarit Karonen ◽  
Jari Sinkkonen ◽  
Marjo Helander ◽  
Juha-Pekka Salminen
Keyword(s):  
Phenolic Compounds ◽  
High Performance ◽  
Betula Pendula ◽  
Silver Birch ◽  
Biologically Active ◽  
Array Detector ◽  
Ionization Mass ◽  
Inner Bark ◽  
First Time

Abstract A method has been developed for the characterization of biologically active silver birch (Betula pendula) inner bark phenolics based on high-performance liquid chromatography/diode array detector (HPLC-DAD)/electrospray ionization-mass spectrometry (ESI-MS). It was demonstrated that the inner bark contains high amounts of flavonoids, arylbutanoids, diarylheptanoids, simple phenolic compounds, phenolic acids, lignans, and procyanidins. Altogether, 30 individual compounds were characterized based on their ultraviolet (UV) and MS data. Structures of 22 compounds were confirmed by nuclear magnetic resonance (NMR) spectroscopy. In addition to previously reported phenolic compounds, 12 compounds were identified in silver birch inner bark for the first time; two of them are novel compounds: 3-β-glucopyranosyloxy-2-hydroxy-1-(4-hydroxy-3-methoxy-phenyl)-propan-1-one and 1,7-bis-(4-hydroxyphenyl)-3-heptanol 3-O-β-ap-iofuranosyl-(1→2)-β-glucopyranoside.

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