scholarly journals Desenvolvimento in vitro da orquídea ameaça de extinção Laelia marginata Lindl. em meios de cultivo contendo diferentes relações nitrato/amônio

2017 ◽  
Vol 23 (4) ◽  
pp. 392-399
Author(s):  
Douglas Junior Bertoncelli ◽  
Guilherme Augusto Cito Alves ◽  
Rodrigo Thibes Hoshino ◽  
Gustavo Henrique Freiria ◽  
Felipe Favoretto Furlan ◽  
...  
Keyword(s):  
Root Length ◽  
Aerial Part ◽  
Culture Media ◽  
Dry Mass ◽  
Leaf Length ◽  
In Vitro Cultivation ◽  
Initial Development ◽  
Randomized Design ◽  
Vitro Development

In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3 - and NH4 + : T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings.

scholarly journals In vitro development of Encyclea cordigera in different concentrations of honey

2015 ◽  
Vol 46 (4) ◽  
pp. 590-592 ◽  
Author(s):  
Cibele Mantovani ◽  
Kathia Fernandes Lopes Pivetta
Keyword(s):  
Culture Media ◽  
Leaf Length ◽  
In Vitro Development ◽  
Randomized Design ◽  
Sucrose Medium ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Set Up ◽  
Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

scholarly journals Iron concentrations in the in vitro cultivation of native Brazilian orchid Schomburgkia crispa

2018 ◽  
Vol 4 (2) ◽  
pp. 93
Author(s):  
Douglas Bertoncelli ◽  
Guilherme Alves ◽  
Gustavo Freiria ◽  
Felipe Furlan ◽  
Helio Neto ◽  
...  
Keyword(s):  
Root Length ◽  
Iron Concentration ◽  
Leaf Length ◽  
In Vitro Cultivation ◽  
Nutritional Requirement ◽  
Initial Development ◽  
Shoot Height ◽  
Shoot Number ◽  
Number Of Leaves

In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.

scholarly journals Plant Growth Regulators in the in Vitro Cultivation of Acmella oleracea (L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 774
Author(s):  
Nastassja Kimberlly Lima ◽  
Eloisa Schneider Da Silva ◽  
Rayane Monique Sete Da Cruz ◽  
Pedro Henrique Riboldi Monteiro ◽  
Glacy Jaqueline Da Silva
Keyword(s):  
Growth Regulators ◽  
Root Length ◽  
Aerial Part ◽  
Large Scale ◽  
Dry Mass ◽  
Fresh Mass ◽  
Limiting Factor ◽  
Naphthalene Acetic Acid ◽  
In Vitro Cultivation

Acmella oleracea is a tropical plant, typical of the northern region of Brazil. The species belongs to the Asteraceae family and has great therapeutic, pharmacological and industrial potential. A limiting factor for the production of this species on a large scale is the short life cycle. The tissue culture programs use synthetic hormones based on cytokinins, such as kinetin and benzylaminopurine (BAP) and auxins such as naphthalene acetic acid (ANA). The objective of this research was to evaluate the effect of growth regulators on the production of Acmella oleracea "in vitro". The experimental test was carried out with control (C), without the addition of growth regulators and five treatments, composed of: (T1) 0.1; (T2) 0.3; (T3) 0.5 mg L-1 kinetin; (T4) 0.1 mg L-1 of BAP and ANA; (T5) 0.5 mg L-1 of BAP and ANA. The experimental design was a completely randomized block in a factorial arrangement with six treatments, three blocks and twenty-five repetitions per block. The evaluated parameters were: germination, root formation, aerial part length, root length, aerial part fresh mass and root fresh mass, aerial part dry mass and root dry mass. The data obtained were subjected to analysis of variance (p <0.05) and compared using the Tukey test. The results showed that kinetin positively contributed to seed germination and aerial part dry mass development. Treatment 1 had the best results for the parameters root length, shoot length and root dry mass.

scholarly journals In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

2021 ◽  
Vol 51 ◽  
Author(s):  
Kaliana Gottschalk de Freitas ◽  
José Carlos Sorgato ◽  
Jackeline Schultz Soares ◽  
Luan Marlon Ribeiro
Keyword(s):  
Plant Height ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Culture Media ◽  
Ventilation System ◽  
Light Condition ◽  
Leaf Length ◽  
In Vitro Growth ◽  
Randomized Design

ABSTRACT Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

scholarly journals Initial Growth of Seedlings of Diferent Pineapple Cultivars in Boa Vista, RR

2020 ◽  
Vol 24 (1) ◽  
pp. 41-46
Author(s):  
Deyse Cristina Oliveira Da Silva ◽  
Sandra Catia Pereira Uchôa ◽  
José Maria Arcanjo Alves ◽  
Valdinar Ferreira Melo ◽  
Cineone Nascimento Da Silva ◽  
...  
Keyword(s):  
Climatic Conditions ◽  
Leaf Length ◽  
Export Market ◽  
In Vitro Cultivation ◽  
Initial Growth ◽  
Vegetative Development ◽  
Percentage Survival ◽  
Randomized Design ◽  
Fruit Growing

Atualmente, no Brasil, o cultivo do abacaxizeiro, em escala comercial, é quase que exclusivamente com as cultivares Smooth Cayenne e Pérola, no entanto, ambas são suscetíveis a fusariose. A inserção de novas cultivares, com aceitação no mercado de exportação e resistente à fusariose é imprescindível para o sucesso da cultura. O objetivo deste estudo foi avaliar o desenvolvimento vegetativo precoce de cinco cultivares de abacaxi derivadas de micropropagação (BRS Ajubá, BRS Imperial, IAC Fantástico, Pérola e BRS Vitória). As mudas foram produzidas e aclimatizadas no Laboratório de Biotecnologia Biomudas, localizado em Venda Nova do Imigrante – ES, sendo posteriormente transportadas para Boa Vista, RR para implantação do experimento, onde foram conduzidas à casa de vegetação, sob condições climáticas controladas. Utilizou-se o delineamento inteiramente casualizado, em esquema de parcelas subdivididas, sendo cinco cultivares de abacaxizeiro (BRS Ajubá, IAC Fantástico, BRS Imperial, Pérola e BRS Vitória), oriundas de micropropagação, aleatorizadas nas parcelas e cinco épocas de avaliação (30, 60, 90, 120 e 150 dias após o transplantio - DAT) nas subparcelas, com quinze repetições. As variáveis de desenvolvimento vegetativo avaliadas foram: altura da planta, diâmetro da roseta, número de folhas, comprimento e largura de folha ”D”, além da percentagem de sobrevivência, ao final do experimento. As cultivares IAC Fantástico e Pérola apresentaram maior desenvolvimento vegetativo em relação à BRS Ajubá, BRS Imperial e BRS Vitória. As cultivares IAC Fantástico e Pérola, em razão da maior precocidade, podem ser transplantadas para local definitivo a partir de 30 DAT das mudas em casa de vegetação, as demais cultivares requerem 150 DAT. Palavras-chave: Aclimatação de Plantas. Crescimento de Mudas. Cultivo in vitro. Fruticultura. Micropropagação de Plantas.   Abstract Currently, the commercial pineapple cultivation in Brazil relies almost exclusively on the cultivars Smooth Cayenne and Pérola. However, both are susceptible to fusariosis. Develment of new cultivars, that are both acceptable to the export market and resistant to fusariosis, is essential for the continued crop success. The objective of this study was to evaluate early vegetative development of five micropropagation-derived pineapple cultivars (BRS Ajubá, BRS Imperial, IAC Fantástico, Pérola and BRS Vitória). Seedlings were produced and acclimatized at the Biomudas Biotechnology Laboratory, Venda Nova do Imigrante, Espírito Santo, Brazil, and were then transported to Boa Vista, Roraima, for the experiments. Here they were placed in a greenhouse under controlled climatic conditions and a completely randomized design implemented, using a subdivided plots scheme, with the micro-porpergation generated seedlings. Location randomized within plots, and evaluation running for five different periods (30, 60, 90, 120 and 150 days after transplant - DAT) in the subplots, with fifteen replicates. Evaluated vegetative development variables weere: plant height, rosette diameter, number of leaves, "D" leaf length and width, as well as percentage survival at experiment end. Cultivars IAC Fantástico and Pérola showed greater vegetative development than BRS Ajubá, BRS Imperial and BRS Vitória. The cultivars IAC Fantástico and Pérola, due to their greater precocity, can be transplanted to a final growth location 30 days after seedling transplantion into a greenhouse, while other cultivars require 150 DAT occur. Keywords: Plants Acclimation . Seedlings Growth. In vitro Cultivation. Fruit Growing. Pineapple Micropropagation .    

scholarly journals Initial Growth of Amburana cearensis in Decomposed Buriti Stem Substrate and Nitrogen Doses

2018 ◽  
Vol 10 (7) ◽  
pp. 497
Author(s):  
Augusto Matias de Oliveira ◽  
Wéverson Lima Fonseca ◽  
Tiago De Oliveira Sousa ◽  
Hingrid Raiany Santos Teixeira ◽  
Fernandes Antonio de Almeida ◽  
...  
Keyword(s):  
Native Species ◽  
Significant Interaction ◽  
Aerial Part ◽  
Slow Growth ◽  
Dry Mass ◽  
Initial Growth ◽  
Initial Development ◽  
Randomized Design ◽  
Completely Randomized Design

One of the difficulties of producing native species seedlings is slow growth. The objective of this study was to evaluate the initial development of Amburana cearensis (Allem.) A. C. Smith in relation to the concentration decomposed buriti stem substratum and nitrogen doses. A completely randomized design was used in a factorial arrangement of 3 × 5 (proportions of decomposed buriti stem × nitrogen doses), with six replicates. The treatments were formulated substrates comprised of decomposed buriti stem manually mixed with soil (Dystrophic Yellow Latosol) in three proportions (0, 25 and 50%) and five nitrogen doses (0, 100, 200, 300 and 400 mg dm-3) applied in treatments. There was no significant interaction between the DRM and ND factors. However, there was an isolated effect for the following variables: Plant height (PH), stem diameter (SD), leaf number (LN), leaf area (LA), dry mass of the aerial part (DMA) and root length (RL). The best concentrations of the substrate for the variables PH, LA and DMA were 25% and 50%, whereas for SD, LN and RL, the best result was 50%. ND and other variables had good responses under recommended doses ranging from a minimum of 264.82 mg dm-3, observed in DMA, and a maximum of 400 mg dm-3, observed in RL. Both factors positively the quality of umburana seedlings.

Relationship between auxins and cytokinins in the growth and organogenesis of Ocimum basilicum L. ‘Grecco a Palla’

2021 ◽  
pp. 1-16
Author(s):  
Felipe Górski ◽  
Geysiane Moreira Gerotti ◽  
Hélida Mara Magalhães
Keyword(s):  
Culture Media ◽  
Stomatal Density ◽  
Chemical Properties ◽  
Large Cell ◽  
Nodal Segments ◽  
In Vitro Cultivation ◽  
Plant Responses ◽  
Cell Layers ◽  
Vitro Development

The in vitro development of a plant is controlled by factors that promote a series of plant responses, which interfere with tissue organogenesis and morphology. For plants of the family Lamiaceae, these factors remain unknown or poorly understood, hindering in vitro cultivation of these plants. The basil cultivar ‘Grecco a palla’ has attractive chemical properties for medicinal, pharmaceutical, and cosmetic industries; however, its production is limited due to the lack of appropriate cultivation conditions. Two types of explants of this species (nodal segments and stem apexes) were grown in culture media with auxin and cytokinin, and their development was followed for 60 days. During in vitro cultivation, both explants were subjected to higher concentrations of plant growth regulators (PGRs) produced only calluses, without induction of shoots. Small amounts of regulators favored hyperhydricity as nodal segments or stem apexes in the absence of PGRs produced plants with disturbances, including brittle, light green, and thick leaves. In this case, there was an increase in the cell layers of palisade parenchyma, which had large cell spaces and larger cells. This tissue also advanced to spongy parenchyma and compressed it. The stomatal density was low; however, the stomata were larger with additions mainly in the guard cells and the stomatic opening. Therefore, stem apexes in the absence of PGRs produced more vigorous plants, whereas nodal segments with low amounts of cytokinins and auxins developed a well-branched and abundant root system.

scholarly journals Organic fertilizer on the in vitro cultivation of the Cattleya labiata orchid

2017 ◽  
Vol 2 (2) ◽  
pp. 62
Author(s):  
Rodrigo Thibes Hoshino ◽  
Ronan Carlos Colombo ◽  
Ana Paula Zandoná ◽  
Guilherme Augusto Cito Alves ◽  
Ricardo Tadeu de Faria
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Average Length ◽  
Organic Fertilizer ◽  
Dry Mass ◽  
In Vitro Cultivation ◽  
Shoot Height ◽  
Organic Products ◽  
Number Of Leaves

Propagation of in vitro plants through other culture media rather than the traditional ones has been widely researched, with satisfactory results. However, to increase the effectiveness of these media, the addition of organic products has been presented satisfactory results. Therefore, the objective of this work was to evaluate the influence of the FishFértil® organic fertilizer on the in vitro cultivation of the Brazilian orchid Cattleya labiata. Treatments included FishFértil® fertilizer concentrations of 0; 1; 2; 3; 4; 5 and 6 mL L-1 in a simplified culture medium. At 180 days, shoot height, number of leaves, leaf area, number of roots, root average length, shoot and root dry mass and the shoot: root ratio were evaluated. The experimental design was entirely randomized, with 10 replications, each one containing 10 plantules. Data were submitted to an analysis of variance and regression analysis, at 5% of significance. The FishFértil® organic fertilizer at the concentration of 6 mL L-1 promoted better plantule growth of Cattleya labiata, subcultivated in vitro, in a simplified culture medium.

scholarly journals Silicon alters early development of bean seedlings from seeds infected by Anthracnose

2021 ◽  
Vol 17 (3) ◽  
pp. 39-51
Author(s):  
Patrícia Migliorini ◽  
Cristina Rossetti ◽  
Andreia da Silva Almeida ◽  
Bruna Barreto Reis ◽  
Gustavo Fonseca Rodrigues ◽  
...  
Keyword(s):  
Early Development ◽  
Root Length ◽  
Aerial Part ◽  
Dry Matter ◽  
Dry Mass ◽  
Colletotrichum Lindemuthianum ◽  
Seed Coating ◽  
Germination Test ◽  
Initial Development ◽  
Seedling Length

The present study aimed to evaluate the effect of seed coating with Si in response to infection in a cultivar susceptible and resistant to Colletotrichum lindemuthianum, on the physiological and health potential of seeds and seedlings, during the initial development of bean culture. The experiment was carried out in a completely randomizeddesign, in a 2 × 2 × 2 factorial scheme, with four replications, where the factors were: cultivar (BRS Esplendor and IPR Tuiuiú), silicate application (with and without Agrosilício® coating) and inoculation (seeds inoculated or not with C. lindemuthianum). In the evaluation of the initial development of the culture, the following variables were measured: germination, first count of the germination test, emergence, emergence speed index (IVE), total seedling length, shoot and root length, total dry mass, aerial part dry matter, root dry matter and aerial part dry matter at 15 days after sowing (DAS). The incidence of C. lindemuthianumin beans was determined by the paper roll method. Covering the seeds with Si reduces the incidence and severity of C. lindemuthianumand provides greater growth of bean seedlings. The effect of responses potentiated by Si is more expressive in the cultivar of greater susceptibility.

scholarly journals Desenvolvimento do capim vetiver cultivado em diferentes ambientes e adubado com esterco bovino

2020 ◽  
Vol 1 (50) ◽  
pp. 130
Author(s):  
Leonardo Oliveira Santos Medeiros ◽  
Maria Teresa Cristina Coelho Do Nascimento ◽  
Joelma Sales dos Dos Santos ◽  
Ilza Maria do Nascimento Brasileiro ◽  
Carlos Alberto Vieira de Azevedo
Keyword(s):  
Root System ◽  
Plant Height ◽  
Root Length ◽  
Aerial Part ◽  
Dry Mass ◽  
Vetiver Grass ◽  
Ambient Conditions ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Free Environment

<p>The objective of this work was to evaluate the development of vetiver grass (Chrysopogon zizanioides L. Roberty) cultivated in PVC columns and fertilized with bovine manure levels under different ambient conditions. The experiment was installed in a completely randomized design, with three replications, in a factorial scheme 5 x 2 being five levels of bovine manure (0%, 10%, 20%, 30% and 40%) and two types of environments (free and protected environment with 80% opening shade), totalizing 30 experimental units. Evaluations were carried out regarding plant height and number of shoots to 15, 30, 45 and 60 days after transplanting (DAT). At 60 DAT at the end of the experiment, the plants were separated to determine the root length and the production of green and dry mass of the aerial part and the root. The results obtained showed that the absence of bovine manure contributed to the greater development of the root system of vetiver grass plants. The 10% level of bovine manure provided a greater number of sprouts and increase in the green mass of the aerial part and green dry mass of the root. The free environment provided the greatest development of vetiver grass plants.</p>

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