scholarly journals Insecticidal Activity of Cyperus rotundus L. and Datura stramonium L. Co-Administered with Sesame Oil Against African Bollworm Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

2021 ◽  
Vol 3 (4) ◽  
pp. 1-8
Author(s):  
Waleed Elamin Elhaj ◽  
Abdelgadir Ahmed Osman ◽  
Loai Mohamed Elamin Elawad
Keyword(s):  
Synergistic Effect ◽  
Ethanolic Extract ◽  
Lethal Effect ◽  
Datura Stramonium ◽  
Sesame Oil ◽  
Cyperus Rotundus ◽  
Agricultural Pests ◽  
Randomized Design ◽  
High Degree ◽  
Lepidoptera Noctuidae

African bollwormis a worldwide-spread species that shows a high degree of polyphagia and it is considered as one of the main agricultural pests in the world. Laboratory experiments were conducted in the Research Laboratory, College of Agricultural Studies, Sudan University of Science and Technology to evaluate the lethal effect of C. rotundusand D. stramonium on the H. armigeraand to investigate the synergistic effect of sesame oil and tested extracts by dipping methods. Five concentrations (4%, 6%, 8%, 10% and 12%) were used for each plant extract in a Completely Randomized Design. Tubers ethanolic extract of C. rotundus at 12% concentration caused 90% mortality after 72 hrs of application, whereas 12% concentration of seeds ethanolic extract of D. stramonium generate only 70% mortality after 72 hrs of exposure. When sesame oil was added to each concentration of ethanolic extract of C. rotundus and D. stramonium it exhibited a synergistic effect. In fact, the 10% concentration scored 53.3% and 76.7% mortality, however, when mixed with sesame oil the mortality increased significantly to 83.3% and 100% for D. stramonium and C. rotundus respectively after 72 hrs of exposure. This study clearly demonstrates that both tested plants have a lethal effect on the larvae of the African bollworm. However, tubers ethanolic extract of C. rotundus seems to be significantly more toxic than the seeds ethanolic extract of D. stramonium. This study also revealed that sesame oil has a synergistic effect when added to these plant extracts.

PENGGUNAAN MEDIA TANAM DAN PUPUK NPK TERHADAP HASIL RUMPUT TEKI (Cyperus rotundus) SERTA ANALISA KANDUNGAN KERIPIK OLAHANNYA

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Wiwik Endah Rahayu ◽  
Atika Romalasari
Keyword(s):  
Research Result ◽  
Nutrient Content ◽  
Cyperus Rotundus ◽  
Root Weight ◽  
Tuber Weight ◽  
Randomized Design ◽  
Two Factors ◽  
The Media ◽  
Npk Fertilizer ◽  
Weight Number

Nut grass is Cyperaceae group and underestimated its existence. Nut grass has many benefits so  potential to be developed. This study aims to determine the effect of planting media and the addition of NPK fertilizer to the growth of the nut grass and find out the nutrient content of chips made from nut grass tuber. The design of this study was factorial complete randomized design (RAL) which consisted of two factors. First factor is Planting Media, consist of M1 = soil, M2 = Soil: Sand (2: 1), M3 = Soil: Compost Fertilizer (2: 1) and M4 = Soil: Cage Fertilizer (2: 1) and second factor is fertilizer dosage NPK 15: 15: 15 with the level P1 = Without NPK fertilizer, P2 = 5 g NPK fertilizer, P3 = 10 g NPK fertilizer. Research result showed that the media significantly affected the number of clumps, root weight, clump weight, overall weight and number of flowers but did not significantly affect the height, number of tubers and tuber weight. Fertilizer significantly affected the number of clumps, root weight, clump weight, overall weight, number of flowers, number of tubers and tuber weight but did not significantly affect the height at P≤0.05 level. Interaction between planting media and NPK fertilizer did not significantly affect all parameters. Keywords: Chips, NPK Fertilizer, Nut Grass, Planting Media

Effect of Plant Essential Oils and Their Major Constituents on Cypermethrin Tolerance Associated Detoxification Enzyme Activities in Spodoptera litura (Lepidoptera: Noctuidae)

2019 ◽  
Vol 112 (5) ◽  
pp. 2167-2176 ◽  
Author(s):  
Torranis Ruttanaphan ◽  
Wanchai Pluempanupat ◽  
Chutikan Aungsirisawat ◽  
Polnarong Boonyarit ◽  
Gaelle Le Goff ◽  
...  
Keyword(s):  
Essential Oil ◽  
Synergistic Effect ◽  
Essential Oils ◽  
Spodoptera Litura ◽  
Cytochromes P450 ◽  
Detoxification Enzymes ◽  
Plant Essential Oils ◽  
Activity Measurements ◽  
Lepidoptera Noctuidae ◽  
Essential Oil Compounds

Abstract Essential oils are well known to act as biopesticides. This research evaluated the acute toxicity and synergistic effect of essential oil compounds in combination with cypermethrin against Spodoptera litura Fabricius (Lepidoptera: Noctuidae). The effects of distillation extracts of essential oils from Alpinia galanga Zingiberaceae (Zingiberales) rhizomes and Ocimum basilicum Lamiaceae (Lamiales) leaves; one of their primary essential oil compounds 1,8-cineole; and linalool were studied on second-instar S. litura by topical application under laboratory conditions. The results showed that A. galanga had the highest control efficiency, whereas1,8-cineole provided a moderate efficacy. The mixtures of linalool, 1,8-cineole, O. basilicum, or A. galanga with cypermethrin were synergistic on mortality. Activity measurements of the main detoxification enzymes show that linalool and 1,8-cineole inhibit the activity of cytochromes P450 and carboxylesterases, which could explain their synergistic effect. Based on our results, the use of these mixtures represents an ideal eco-friendly approach, helping to manage cypermethrin resistance of S. litura.

scholarly journals Efektifitas Pestisida Biologis Bacillus Cereus dan Bacillus Megaterium. sebagai Pengendali Spodoptera litura Fabr (Lepidoptera: Noctuidae)

2017 ◽  
Vol 17 (2) ◽  
Author(s):  
I Made Indra Agastya ◽  
Aminudin Afandhi ◽  
Luqman Qurata Aini
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Megaterium ◽  
Spodoptera Litura ◽  
Laboratory Studies ◽  
Pests And Diseases ◽  
Control Effectiveness ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Lepidoptera Noctuidae

This research was conducted in vitro in the laboratory. Studies conducted in the Laboratory of Bacteriology Department of Plant Pests and Diseases of the Faculty of Agriculture, University of Brawijaya, on the effectiveness of the bacteria Bacillus cereus and Bacillus megaterium as biological pesticides controlling Spodoptera litura. The objective of this study was to determine the effectiveness of the bacteria Bacillus sp as biological control. Effectiveness pesticide was measured by testing the incubation period and mortality in larvae of S. litura instar 3. This study used a completely randomized design (CRD), conducted observations every 6 hours until the larvae dead. The results showed that the percentage of mortality of S. litura reached 94.66% compared to the control, while the incubation time of the bacteria B. cereus cause disease until 29.84 hours. B. cereus and B. megaterium have the ability to incubate third instar larvae of S. litura up to 29.84 hours and caused the death of larvae up to 94.66%. Keywords: Biological pesticides, Bacillus cereus, Bacillus megaterium, entomopatogen, Spodoptera litura

scholarly journals UTILIZATION OF PAPAYA LEAVES (Carica papaya L.) TO CONTROL ONION PEST SPODOPTERA EXIGUA (LEPIDOPTERA: NOCTUIDAE)LEPIDOPTERA (NOCTUIDAE)

2020 ◽  
Vol 7 (1) ◽  
pp. 1-7
Author(s):  
Muhammad Agung Permadi ◽  
Rafiqah Amanda Lubis ◽  
Syawaludin Syawaludin ◽  
Nova Suryani Pasaribu
Keyword(s):  
Experimental Method ◽  
Carica Papaya ◽  
Spodoptera Exigua ◽  
Toxic Compounds ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Lepidoptera Noctuidae

Spodoptera exigua is a migrant pest that attacks onions simultaneously in groups. The utilization of papaya leaves to control pests due to papaya leaves have toxic compounds that can kill pests. This study aims to measure the ability of papaya leaves to control S. exigua larvae. This research was conducted using a completely randomized design (CRD) experimental method with two treatment factors. The first factor was papaya leaf concentration (0 g / l, 30 g / l, 70 g / l, 100 g / l, 120 g / l), and the second factor is a mixture of kerosene. The treatment was repeated three times. The study was conducted at the Agrotechnology Laboratory, Agriculture Faculty Muhammadiyah University, South Tapanuli. The results showed that papaya leaves could kill S. exigua larvae. The highest mortality of S. exigua worms There were statistically significant non-treatments among treatments.

scholarly journals Effect of Food Attractants and Insecticide Toxicity for the Control of Spodoptera frugiperda (Lepidoptera: Noctuidae) Adults

2019 ◽  
Vol 12 (1) ◽  
pp. 129
Author(s):  
Wagner Justiniano ◽  
Marcos Gino Fernandes
Keyword(s):  
Spodoptera Frugiperda ◽  
Sucrose Solution ◽  
Feeding Time ◽  
Dietary Preference ◽  
Hydrolyzed Protein ◽  
Randomized Design ◽  
Lambda Cyhalothrin ◽  
Effect Of Food ◽  
Food Attractants ◽  
Lepidoptera Noctuidae

An alternative for the population suppression of Spodoptera frugiperda is the use of toxic baits. The objective of this study was to evaluate the dietary preference and toxic effect of insecticides associated with the attractiveness of S. frugiperda adults as a pest management method. The following attractions were tested: 1) 5% sucrose solution, 2) 10% sugarcane syrup, 3) 10% honey, 4) 5% hydrolyzed protein, 5) Noctovi® 43sb, 6) Noctovi® OVI PLU 1-3, 7) Noctovi® OVI PHE/PAL 50-50 in the study with choice. Attractions: 1) sugarcane syrup 10%, 2) Noctovi® 43sb, 3) Noctovi® 43sb + sugarcane syrup 10%, 4) Noctovi® OVI PLU 1-3 in the study with no chance of choice. For the toxicity study, the food attraction associated with insecticides was used: methomyl 2%, lambda-cyhalothrin 1%, chlorpyriphos 2%, spinosad 1%, chlorantraniliprole 2% and chlorfenapyr 2%. The experimental design was the completely randomized design (CRD) with chance of choice, without chance of choice and toxicity. The following parameters were evaluated: number of insects that fed; time in minutes that remained in the attractive food and mortality. The molasses (10%) and Noctovi® 43sb food attractiveness were significantly more efficient in relation to feeding time and the highest number of landings was observed in the Noctovi® 43sb treatment, both in females and total adults. Methomyl, lambda-cyhalothrin and spinosad insecticides associated with food attractiveness are promising toxic baits for the management of S. frugiperda.

Secondary Metabolites of Asclepias curassavica (Apocynaceae) and its Effects on Food Preference and Mortality of Spodoptera frugiperda (Lepidoptera: Noctuidae)

2020 ◽  
pp. 583
Author(s):  
Renato Marcos De Leão ◽  
João Vitor Souza Cruz ◽  
Vânia Maria Ramos ◽  
Viviane Tavares De Almeida ◽  
Pedro Henrique Gorni ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Food Consumption ◽  
Spodoptera Frugiperda ◽  
Food Preference ◽  
Ethanolic Extract ◽  
Parallel Analysis ◽  
Total Polyphenols ◽  
Asclepias Curassavica ◽  
Preference Index ◽  
Lepidoptera Noctuidae

Asclepias curassavica L. plant is toxic for vertebrates, and little is known about its effects on invertebrates, as well as whether its secondary metabolites have an influence on food preference and survival of insects. Thus, a study was conducted to verify the action of A. curassavica on Spodoptera frugiperda J.E.Smith. The plants were collected, dried and ground to compose a crude ethanolic extract. Food tests were carried out with and without opportunity of choice, using corn leaf discs immersed in the extract at concentrations of 1%, 2% and 4%, for assessment of food consumption (g) and preference index. Later, the extract was topically applied on second- and fifth-instar caterpillars, at concentrations of 1%, 2%, 4% and 6%, in order to observe its effect on survival. In parallel, analysis was conducted to verify the presence and measure the amount of total polyphenols and flavonoids in the extract. There was contact action between the extract and second-instar caterpillars at all concentrations, with treatment 6% causing 100% of mortality 72 hours after application. None of the treatments promoted contact action on fifth-instar caterpillars. In food tests with and without opportunity of choice, all treatments caused reductions in consumption, which classified all as phagodeterrent, with treatment 4% standing out as the least favorite. The presence of total polyphenols and flavonoids was found in the extract, presenting 58.75 ug/mL and 150.1 ug/mL, respectively. The A. curassavica extract proved promising in S. frugiperda control.

scholarly journals Combinational Effect of Rumex tingitanus (Polygonaceae) Hexane Extract and Bacillus thuringiensis  δ-Endotoxin against Spodoptera littoralis (Lepidoptera: Noctuidae)

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Dhekra Mhalla ◽  
Dalel Ben Farhat-Touzri ◽  
Slim Tounsi ◽  
Mohamed Trigui
Keyword(s):  
Biological Activity ◽  
Chemical Composition ◽  
Bacillus Thuringiensis ◽  
Synergistic Effect ◽  
Spodoptera Littoralis ◽  
Insect Pests ◽  
Hexane Extract ◽  
Active Fraction ◽  
Lepidoptera Noctuidae ◽  
Potential Use

The increasing insect resistance against Bacillus thuringiensis delta-endotoxins is a serious problem which makes it urgent to look for new eco-friendly strategies. Combining these toxins with other biomolecules is one of the promising strategies against insect pests. In this work, we evaluated the bioinsecticidal potential of Rumex tingitanus extracts and B. thuringiensis strain BLB250 against Spodoptera littoralis (Lepidoptera: Noctuidae) larvae. The chemical composition of the hexane extract, the most active fraction, was analyzed to validate the correlation between chemical composition and biological activity. Among the tested extracts, only the hexanic extract showed toxicity against first and second instar larvae with LC50 of 2.56 and 2.95 mg g−1, respectively. The Bacillus thuringiensis BLB250 delta-endotoxins showed toxicity with an LC50 of 56.3 μg g−1. Therefore, the investigated combinational effect of BLB250 delta-endotoxins and R. tingitanus hexane extract proved significant synergistic effect against S. littoralis larvae. The GC-MS analysis of R. tingitanus hexane extract showed the richness of this extract in phytosterols such as β and γ-sitosterol (48.91%), campesterol (6.43%), and β-amyrin (8.92%) which are known for their insecticidal activity. This novel finding highlights the potential use of this combination against insect pests to prevent the appearance of resistance problems.

Bacterial Resistance to Ultrasonic Waves under Pressure at Nonlethal (Manosonication) and Lethal (Manothermosonication) Temperatures

1999 ◽  
Vol 65 (1) ◽  
pp. 297-300 ◽  
Author(s):  
R. Pagán ◽  
P. Mañas ◽  
J. Raso ◽  
S. Condón
Keyword(s):  
Heat Treatment ◽  
Listeria Monocytogenes ◽  
Synergistic Effect ◽  
Aeromonas Hydrophila ◽  
Salmonella Enteritidis ◽  
Bacterial Resistance ◽  
Lethal Effect ◽  
Ultrasonic Waves ◽  
Relative Pressure ◽  
Lethal Effects

ABSTRACT The decimal reduction times of Streptococcus faecium,Listeria monocytogenes, Salmonella enteritidis, and Aeromonas hydrophila corresponding to heat treatment at 62°C were 7.1, 0.34, 0.024, and 0.0096 min, and those corresponding to manosonication treatment (40°C, 200 kPa, 117 μm) were 4.0, 1.5, 0.86, and 0.90 min, respectively. The manosonication decimal reduction times of the four species investigated decreased sixfold when the amplitude was increased from 62 to 150 μm and fivefold when the relative pressure was raised from 0 to 400 kPa. In L. monocytogenes, S. enteritidis, and A. hydrophila, the lethal effect of manothermosonication was the result of the addition of the lethal effects of heat and manosonication, whereas in S. faecium it was a synergistic effect.

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