Possible protective effect of olive leaves extract on paracetamol induced hepatotoxicity in male albino rats

Paracetamol (PCM) overdose can cause hepatotoxicity with oxidative stress; the present study was carried out to establish the possible protective effect of olive leaves extract (OLE) on toxicity induced by paracetamol in adult male rats. Twenty four adult male rats were divided into four equal groups; control, olive leaves extract group, paracetamol group and olive leaves extract plus paracetamol group. Some biochemical parameters and liver histopathology were evaluated. PCM treatment significantly increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, gamma-glutamyltransferase (GGT), lactate dehydrogenase (LDH), urea, creatinine and alpha-fetoprotein. Paracetamol was found to significantly increase malonaldehyde (MDA) and decrease glutathione reductase (GR) activity in tissue and significantly decrease total antioxidant capacity (TAC) and superoxide dismutase (SOD) in serum. Administration of OLE caused a significant decrease serum AST, ALT enzyme, total bilirubin, GGT, LDH, creatinine, urea, alpha-fetoprotein. Also, amelioration of oxidant – antioxidant status with olive leaves extract was observed in addition to a significant decrease in MDA and a significant increase in TAC in liver tissue with a significant increase in glutathione reductase (GR) and SOD in serum compared to paracetamol treated group The chemical pathological changes were in step with histopathological observation suggesting marked hepatoprotective result of olive leaves extract. It could be concluded that olive leaves extract (OLE) treatment may be effective in decreasing hepatic injury and oxidative stress induced by paracetamol overdose in male albino rats.

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Experimental Comparative Study of potential anxiolytic effect of Vitamin C and Buspirone in rats

Functional Foods in Health and Disease ◽

10.31989/ffhd.v8i2.365 ◽

2018 ◽

Vol 8 (2) ◽

pp. 91

Author(s):

Ghada Farouk Soliman ◽

Aida Abdalla Khattab ◽

Mariam Refaat Habil

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Glutathione Reductase ◽

Open Field ◽

Corticosterone Level ◽

Anxiolytic Effect ◽

Treated Group ◽

Percentage Change ◽

Albino Rats ◽

Behavioral Tests

Background: Anxiety disorders are the most common of all mental health problems. They are more prevalent among women than among men, and they affect children as well as adults. The aim of the current study is to evaluate this problem via an experimental animal model and try to explore its possible mechanisms by studying the effect of Vitamin C compared to Buspirone on anxiety in rats induced by Monosodium Glutamate (MSG).Materials and Methods: 56 healthy adult male albino rats (Sprague-Dawley) weighing 200-250 gm were used and divided into 7 groups (8 rats each). The first and the second groups were provided with normal saline and MSG at a dose of (2 mg/g p.o.) respectively. The other five groups were given MSG and treated daily in the following way: The third and fourth groups were treated with Vitamin C (100, 200 mg/kg p.o) respectively. The fifth group was treated with only Buspirone (10 mg/kg p.o.), while the last sixth and seventh groups were given a combination of Buspirone and Vitamin C with (100, 200 mg/kg) respectively. After 3 weeks, the open field and successive alleys tests were used to assess behavioral changes. The percentage change of systolic blood pressure (SBP) was measured. Additionally, glutathione reductase (GR), malondialdehyde (MDA), and corticosterone levels were determined biochemically.Results: The results after 3 weeks revealed that MSG group showed significant anxiogenic effects in both behavioral tests, with an increased percentage change of SBP in addition to increased malondialdehyde and corticosterone level measured statistically. While the results of the treated groups revealed that the Vitamin C (100mg/kg) treated group demonstrated significant improvement in anxiety levels in the open field test, there were no significant changes in the biochemical assessment. However, vitamin C (200mg/kg) treated group revealed a significant anxiolytic effect in behavioral tests, improved glutathione and malondialdehyde with low corticosterone level. Administration of buspirone revealed significant anxiolytic effects, which is lower than that of vitamin C (200mg/kg). But it caused significant increase in the oxidative stress and corticosterone levels. A combination of buspirone with Vitamin C (200mg/kg) only demonstrated significant anxiolytic effect in both tests and a significant decrease of corticosterone.Conclusion: MSG has neurotoxic effect leading to anxiogenic behaviors in rats which are opposed by Vitamin C. Furthermore, as an antioxidant, vitamin C protects against the oxidative stress induced by MSG. Moreover, it lowers the high corticosterone level associated with MSG or buspirone administration.Key Words: MSG, vitamin C, buspirone, glutathione reductase, malondialdehyde, open field, successive alleys

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Ameliorating Iron Overload in Intestinal Tissue of Adult Male Rats: Quercetin vs Deferoxamine

Journal of Toxicology ◽

10.1155/2018/8023840 ◽

2018 ◽

Vol 2018 ◽

pp. 1-13 ◽

Cited By ~ 1

Author(s):

Arwa A. El-Sheikh ◽

Shimaa Hamed Ameen ◽

Samaa Salah AbdEl-Fatah

Keyword(s):

Oxidative Stress ◽

Iron Overload ◽

Adult Male ◽

Caspase 3 ◽

Iron Chelation ◽

Male Rats ◽

Albino Rats ◽

Tissue Iron ◽

Small Intestinal

Objective. The aim of our study is to compare the role of the new natural alternative (Quercetin) with the current iron-chelation therapy (Deferoxamine (DFO)) in the effect of iron overload on small intestinal tissues and to investigate the possible underlying molecular mechanisms of such toxicity. Methods. Forty-two adult male albino rats were divided into six groups: control groups, DFO, Quercetin, iron overload, iron overload+DFO, and iron overload+Quercetin groups. Animals received daily intraperitoneal injection of Deferoxamine (125 mg /kg), Quercetin (10 mg/kg), and ferric dextran (200 mg/kg) for 2 weeks. Results. Iron overloaded group showed significant increase in serum iron, total iron binding capacity (TIBC), transferrin saturation percentage (TS %) hepcidin (HEPC), serum ferritin, nontransferrin bound iron (NTBI), and small intestinal tissues iron levels. Iron overload significantly increased the serum oxidative stress indicator (MDA) and reduced serum total antioxidant capacity (TAC). On the other hand, iron overload increased IL6 and reduced IL10 in small intestinal tissues reflecting inflammatory condition and increased caspase 3 reactivity indicating apoptosis and increased iNOs expressing cell indicting oxidative stress especially in ileum. In addition, it induced small intestinal tissues pathological alterations. The treatment with Quercetin showed nonsignificant differences as compared to treatment with DFO that chelated the serum and tissue iron and improved the oxidative stress and reduced tissue IL6 and increased IL10 and decreased caspase 3 and iNOs expressing cells in small intestinal tissues. Moreover, it ameliorated the iron overload induced pathological alterations. Conclusion. Our study showed the potential role of Quercetin as iron chelator like DFO in case of iron overload induced small intestinal toxicity in adult rats because of its serum and tissue iron chelation, improvement of serum, and small intestinal oxidative stress, ameliorating iron induced intestinal inflammation, apoptosis, and histopathological alterations.

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Chronic Addiction to Tramadol and Withdrawal Effect on the Spermatogenesis and Testicular Tissues in Adult Male Albino Rats

Pharmacology ◽

10.1159/000496424 ◽

2019 ◽

Vol 103 (3-4) ◽

pp. 202-211 ◽

Cited By ~ 3

Author(s):

Marwan Abdel-Latif Ibrahim ◽

Alaa-Eldin Salah-Eldin

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Adult Male ◽

Rna Extraction ◽

B Cell Lymphoma ◽

Experimental Period ◽

Male Rats ◽

Control Group ◽

Albino Rats ◽

Withdrawal Effect

Aim: The present study aimed to elucidate the effects of tramadol on the testicular functions of adult male rats due to the chronic usage of tramadol and the effect of its withdrawal. Method: Adult male albino rats were classified into the following 3 groups: (I) a control administered with normal saline and (II) tramadol-treated rats (40 mg/kg b.w. orally) for 21 successive days; and (III) like the rats in the second group but kept for 4 weeks after the last tramadol dose to study the effect of tramadol withdrawal. At the end of the experimental period, blood was collected and specimens from testis were taken for histopathological, biochemical, and molecular studies. A reverse transcription-polymerized chain reaction after RNA extraction from specimens was detected for the anti-apoptotic and pro-apoptotic genes in testicular tissues. Also, malondialdehyde (MDA) was measured in tissues homogenate and antioxidant enzymes activities were evaluated. Results: The results of this study demonstrated histological changes in testicular tissues in groups II and III compared to the control group, accompanied with increased apoptotic index and proved by increased B-cell lymphoma-2 (Bcl-2) associated-X-protein and caspase-3 expression, whereas anti-apoptotic Bcl-2 markedly decreased. Moreover, in tramadol-abused and -withdrawal groups, the MDA level increased, while the antioxidant enzymes activity decreased and revealed oxidative stress, indicating that tramadol is harmful at the cellular level and can induce apoptotic changes in testicular tissues. The withdrawal effect showed signs of improvement, but it did not return to normal levels. Conclusions: It could be concluded that the administration of tramadol causes abnormalities on testicular tissues associated with oxidative stress, which confirmed the risk of increased oxidative stress on testicular tissues due to tramadol abuse.

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Protective effect of aqueous seed extract of Vitis Vinifera against oxidative stress, inflammation and apoptosis in the pancreas of adult male rats with diabetes mellitus

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2016.04.032 ◽

2016 ◽

Vol 81 ◽

pp. 439-452 ◽

Cited By ~ 18

Author(s):

Siti Hajar Adam ◽

Nelli Giribabu ◽

Normadiah Kassim ◽

Kilari Eswar Kumar ◽

Manuri Brahmayya ◽

...

Keyword(s):

Oxidative Stress ◽

Diabetes Mellitus ◽

Protective Effect ◽

Vitis Vinifera ◽

Adult Male ◽

Seed Extract ◽

Male Rats

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Hepatoprotective role of Solenostemma argel growing in Egypt on ethanol induced oxidative damage in rats

Turkish Journal of Biochemistry ◽

10.1515/tjb-2016-0211 ◽

2017 ◽

Vol 42 (4) ◽

Author(s):

Mahgoub Mohamed Ahmed

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Oxidative Damage ◽

Protective Effect ◽

Rat Liver ◽

Adult Male ◽

Total Protein ◽

Albino Rats ◽

Serum Total Protein

AbstractObjective:The objective of the current study is to investigate the protective effect ofMethods:Forty adult male albino rats were divided into four groups as control,Results:The results showed that, administration of EtOH caused a significant decrease (p<0.05) in serum total protein and albumin, whereas ALT and AST and lipid peroxidation (LPO) were increased following EtOH treatment.Conclusion:had a hepatoprotective role against EtOH-induce oxidative stress and inflammation in rat liver.

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A Histomorphometric Study of the Effect of Monosodium Glutamate on the Ileum of Adult Male Albino Rats and the Possible Protective role of Vitamin E

QJM ◽

10.1093/qjmed/hcab085.001 ◽

2021 ◽

Vol 114 (Supplement_1) ◽

Author(s):

Heba Mahmoud Aly ◽

Kawther Ahmed Hafez ◽

Iman Hussein Abdel Aal ◽

Youssef Shoukry Abdel Aal ◽

Shereen Adel Saad

Keyword(s):

Vitamin E ◽

Protective Effect ◽

Olive Oil ◽

Adult Male ◽

Microscopic Examination ◽

Monosodium Glutamate ◽

Goblet Cells ◽

Treated Group ◽

Albino Rats ◽

Once Daily

Abstract Background Monosodium Glutamate (MSG) is one of the most widely used food-additives in commercial foods. Its application has increased over time and it is found in many different ingredients and processed foods obtainable in every market or grocery store. Besides its flavor enhancing effects, MSG has been associated with various forms of toxicity. Aim of the work The aim of the present work was to determine the effect of monosodium glutamate on the histology of the ileum of the adult male albino rats and evaluate the protective effect of vitamin E. Material and methods 30 Adult male albino rats were assigned randomly into three groups; control group, it was further subdivided into three subgroups (IA received no treatment, IB received 2 ml olive oil/day, and IC received 400 IU/kg. BW of Vitamin E dissolved in 2 ml olive oil once daily), MSG group (rats fed 2mg/kg. BW of MSG once daily, orally), and vitamin E treated group (rats fed MSG and received concomitant 400 IU/kg/day vitamin E orally). Weight of rats were measured at the start & end of experiment. At the end of experiment (15 days), rats were euthanized, and ileal specimens were processed into paraffin blocks for light microscopic examination and other specimens were processed into scanning electron microscopic examination. Morphometric study and statistical analysis were done. Results The present work demonstrated that MSG induced several histopathological changes of the ileum. Broad, fused villi sloughed epithelial cells and pronounced increase in the number of goblet cells. Massive lymphocyte infiltration and hemorrhage was noticed in the lamina propria. Enterocytes lining crypts showed cytoplasmic vacuolation, pale nuclear staining and loss of demarcation between adjacent cells. Increased villus width, goblet cell and lymphocytes numbers was demonstrated by histomorphometry. Vitamin E treated group showed histopathological findings mostly normalized compared with MSG group. Width and length of villi was reduced, enterocytes appeared healthy and well arranged, reduced inflammatory cell infiltrate and vascular congestion and decreased number of goblet cells was observed. Conclusion The present results demonstrated deleterious effects of MSG on the structure of the mucosa of the ileum. It also suggested a novel and favorable protective effect of vitamin E on mucosa of the ileum.

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The protective effect of the aqueous extract of parsley (Petroselinum sativum ) seeds on experimentally – induced oxidative stress in rats

The Iraqi Journal of Veterinary Medicine ◽

10.30539/ijvm.v25i1.1157 ◽

2021 ◽

Vol 25 (1) ◽

pp. 154-172

Author(s):

K. K. Khudiar ◽

B. N. Abdullah ◽

K. A. Al-Mzaien

Keyword(s):

Oxidative Stress ◽

Drinking Water ◽

Protective Effect ◽

Aqueous Extract ◽

Plasma Cholesterol ◽

Male Rats ◽

Cholesterol Concentration ◽

Control Group ◽

Albino Rats ◽

Group I

In this study, the potential protective effect of aqueous extract of parsley (Petroselinum sativum) seeds against hydrogen peroxide (H2O2) – induced oxidative stress in male rats was assessed. Three groups of male albino rats were randomly divided (n=7) and were handled for twenty-eight days as follows: rats in group I served as control; animals in group || were provided with drinking water containing 0.5% H2O2 and those in group III received orally 8 mg/100 gm B.W. of aqueous extract of parsley seeds plus 0.5% H2O2 in drinking water. After four weeks experimental period, a significant increase in lipid peroxidation products (MDA), and decrease in glutathione (GSH) concentrations were observed in plasma, kidney, liver and heart tissues of H2O2 treated animals as compared with the control group. These biomarkers (GSH and MDA) are interrelated and indicate the occurrence of oxidative stress. Plasma total cholesterol (TC) concentration was significantly increased in H2O2 treated rats. By administration of aqueous extract of parsley along with H2O2, plasma and tissue GSH levels were significantly increased while the elevation in MDA level was diminished in plasma and different tissues examined. A decrease in plasma cholesterol concentration was recorded in H2O2 and parsley treated group as compared with the control one and H2O2 treated groups. These results indicate that aqueous extract of parsley have hypocholesterolemic and antioxidant effect.

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Silymarin mitigates diclofenac-induced liver toxicity through inhibition of inflammation and oxidative stress in male rats

Journal of Herbmed Pharmacology ◽

10.15171/jhp.2019.34 ◽

2019 ◽

Vol 8 (3) ◽

pp. 231-237 ◽

Cited By ~ 2

Author(s):

Pantea Ramezannezhad ◽

Ali Nouri ◽

Esfandiar Heidarian

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Total Bilirubin ◽

Liver Toxicity ◽

Treated Group ◽

Male Rats ◽

Control Group ◽

Tnf Α ◽

Ameliorative Effect ◽

And Oxidative Stress

Introduction: Diclofenac (DIC) is one of the compounds derived from acetic acid which isknown for its anti-inflammatory and analgesic attributes. Silymarin is a flavonoid compoundwhich is derivate from Silybum marianum seeds. This research was done to assess the protectiverole of silymarin against liver toxicity induced by DIC in male rats.Methods: Randomly, 40 male Wistar rats were assigned into five groups as follows: Group 1:control group, Group 2: DIC-only treated (50 mg/kg, i.p), Group 3: silymarin-only treated (200mg/kg, p.o); Groups 4 and 5: DIC (50 mg/kg, i.p) plus silymarin (100 mg/kg and 200 mg/kg, p.o,respectively) treated. Various biochemical, molecular, and histological parameters were evaluatedin serum and tissue.Results: In the DIC-only treated group, the levels of liver glutathione peroxidase (GPx), superoxidedismutase (SOD), intracellular glutathione (GSH) and catalase (CAT) significantly diminished andthe levels of total bilirubin, alkaline phosphatase (ALP), nitrite, alanine aminotransferase (ALT),malondialdehyde (MDA), serum tumor necrosis factor-α (TNF-α), aspartate aminotransferase(AST), and TNF-α gene expression were remarkably elevated relative to control animals. In otherhands, treatment with silymarin caused a noticeable elevation in GPx, SOD, GSH, CAT and aremarkable reduction in levels of total bilirubin, ALP, nitrite content, ALT, MDA, serum TNF-α,AST and TNF-α gene expression relative to DIC-only treated group. Histopathological injurieswere also improved by silymarin administration.Conclusion: The results confirm that silymarin has an ameliorative effect on liver toxicity inducedby DIC and oxidative stress in male rats.

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Diuretic effects of aqueous extract of Olive Leaves (Olea europea) in adult male rats.

The Iraqi Journal of Veterinary Medicine ◽

10.30539/ijvm.v27i1.1096 ◽

2003 ◽

Vol 27 (1) ◽

pp. 50-60

Author(s):

B. N. Abdullah ◽

K. K. Khudiar ◽

B. S. Toma

Keyword(s):

Aqueous Extract ◽

Adult Male ◽

Filtration Rate ◽

Treated Group ◽

Male Rats ◽

Control Group ◽

Olive Leaves ◽

Olea Europea ◽

Blood And Urine Samples ◽

Kidney Functions

This study was carried out to investigate the effects of aqueous extract of olive leaves (Olea europea) on kidney functions. Eighteen adult male rats three equal groups placed individually in were randomly divided in to metabolic cages and were handled as follows: group (C) served as control group; rats in group (T1) were intubated (90 mg/kg B.W.) of aqueous extract of group T2 were intubated (0.8 mg/kg B.W.) of olive leaves and rats in ammuretic. Blood and urine samples were collected after 24 hours of significant increase in urinary output intubation. The results showed a ions and potassium urinary sodium increase in an by accompanied concentration in the olive leaves treated group only. Creatinine clearance (as an for glomerular filtration rate) increased significantly in both treated index groups, it is concluded that olive leaves extract may cause its diuretic effect by. increasing the glomerulor filtration rate.

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