Anti-thrombotic activity of fermented rice bran extract with several oriental plants in vitro and in vivo

During investigations; homology model of 3D-structure was built for sequence of polyhedrin protein of Helicoverpa armigera nucleopolyhedrovirus, containing 246 amino acids (Accession: ACI05106.1 GI: 205946055), and evaluated through multiple tools/ applications to judge extent of accuracy in light of existing crystal structure. Further, in vivo experiments were conducted and determined response of different adjuvants with HaNPV and their efficacy. The pooled mean mortality of larvae exposed to virus mixed with 5% green tea and 5% rice bran filtrates (8.3 larvae per 25 plants) was differ significantly from control (15.8 larvae per 25 plants), suggesting that UV protectants & diet enhancer (mannitol) has ability to protect stability of virulence of the virus, under field conditions. The minimum percent pod damage of 8.6% and maximum yield of 1604.8 Kg ha-1 at harvesting was recorded with formulation of indigenous BHA virus isolate @ 2.2 x 105 POBs mL-1 mixed with Roket @50 ppm; followed by formulation with mannitol (@ 1% + green tea 5% + 5% rice bran filtrates) with percent pod damage of 16.8 % and yield of 1045.8 Kg ha-1 of chickpea. Furthermore, in vitro toxicity of fresh virus suspension @ 250 mL ha-1 was recorded more toxic in terms of percent mortality and LT50 (5.65 days). However, three months stored HaNPV formulations [(A) mannitol @ 1%+ green tea@ 5% and (B) mannitol @ 1% + green tea 5% + 5% rice bran filtrates] were more effective in larval reduction with LT50 of 7.89 and 6.00 days, respectively. Virus mixed with 5% green tea and 5% rice bran filtrates gave stability to formulation up-to one year with LT50 of 7.64 days. Findings showed that HaNPV formulations with mannitol (B) have potential that can be used in integrated manner with other IPM practices, to reduce the use of toxic synthetic pesticides in chickpea.

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In-vitro Free Radicals Scavenging and Antioxidant Activity of Rice Bran Extract

Pharmacologia ◽

10.5567/pharmacologia.2015.377.385 ◽

2015 ◽

Vol 6 (8) ◽

pp. 377-385

Author(s):

Subhash Laxmanrao Bodhankar ◽

Amol Pramod Muthal ◽

Supada Rambhau Rojatkar

Keyword(s):

Antioxidant Activity ◽

Free Radicals ◽

Rice Bran ◽

Rice Bran Extract ◽

Free Radicals Scavenging

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Self-Nanoemulsifying Drug Delivery Systems (SNEDDS) Containing Rice Bran Oil for Enhanced Fenofibrate Oral Delivery: In Vitro Digestion, Ex Vivo Permeability, and In Vivo Bioavailability Studies

AAPS PharmSciTech ◽

10.1208/s12249-020-01765-2 ◽

2020 ◽

Vol 21 (6) ◽

Author(s):

Christina Karavasili ◽

Ioannis I. Andreadis ◽

Maria P. Tsantarliotou ◽

Ioannis A. Taitzoglou ◽

Paschalina Chatzopoulou ◽

...

Keyword(s):

Drug Delivery ◽

Rice Bran ◽

Oral Delivery ◽

Drug Delivery Systems ◽

Rice Bran Oil ◽

Ex Vivo ◽

In Vitro Digestion ◽

Delivery Systems

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Stabilized rice bran extract: Acute and 28-day repeated dose oral toxicity with in vitro mutagenicity and genotoxicity study

African Journal of Pharmacy and Pharmacology ◽

10.5897/ajpp2015.4369 ◽

2015 ◽

Vol 9 (44) ◽

pp. 1037-1050

Author(s):

El Askary Hisham ◽

B Zickri Maha ◽

M Helal Amr ◽

A Heikal Ola ◽

L Fiebich Bernd ◽

...

Keyword(s):

Rice Bran ◽

Repeated Dose ◽

Oral Toxicity ◽

Rice Bran Extract ◽

Dose Oral

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Immunostimulatory Activity of Black Rice Bran in Cyclophosphamide-Induced Immunosuppressed Rats

Natural Product Communications ◽

10.1177/1934578x20934919 ◽

2020 ◽

Vol 15 (7) ◽

pp. 1934578X2093491

Author(s):

Young Mi Park ◽

Hak Yong Lee ◽

Dong Yeop Shin ◽

Yang Hee Lee ◽

Ye Jin Yang ◽

...

Keyword(s):

Rice Bran ◽

White Blood Cells ◽

Serum Levels ◽

Biologically Active ◽

Black Rice ◽

Immunostimulatory Activity ◽

Thymic Tissue ◽

Black Rice Bran

Black rice bran extract (BRBE), containing various biologically active compounds, such as anthocyanin, has antioxidant activity and numerous pharmacological effects. Here, we aimed to confirm the immunostimulatory effects of BRBE in cyclophosphamide (CP)-induced immunosuppressed cells. Our results confirmed that BRBE exerted an immunostimulatory effect. In vitro, BRBE treatment enhanced cell proliferation, activity of natural killer cells and cytotoxic T lymphocytes, and production of CP-repressed cytokines, such as tumor necrosis factor-α, interferon-γ, interleukin (IL)-2, and IL-12, and immunoglobulins G and A in isolated splenocytes. Additionally, in vivo, BRBE treatment increased the number of immune cells, such as white blood cells, lymphocyte counts, mid-range absolute counts, and neutrophils in CP-induced immunosuppressed rats. Furthermore, BRBE increased the serum levels of abovementioned inflammatory cytokines and immunoglobulins in CP-induced immunosuppressed rats. In addition, BRBE protected against CP-mediated spleen and thymic tissue damage. Our findings suggest that BRBE could be potentially used as a component of functional food for immunity enhancement.

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Polysaccharides from Ganoderma Sinense - rice bran fermentation products and their anti-tumor activities on non-small-cell lung cancer

BMC Complementary Medicine and Therapies ◽

10.1186/s12906-021-03346-7 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Wei Han ◽

Hongjuan Chen ◽

Lin Zhou ◽

Haijie Zou ◽

Xiaohong Luo ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Rice Bran ◽

Cell Lung Cancer ◽

Small Cell ◽

Small Cell Lung ◽

Fermentation Products ◽

Ganoderma Sinense

Abstract Background Non-small-cell lung cancer (NSCLC) accounts more than 80% of the lung cancer cases. Polysaccharides in rice bran and its fermentation products have been proven to suppress many cancers. However, the report on inhibiting NSCLC is few. In this paper, the polysaccharides with suppression activity to H1299 NSCLC in the fermentation products of full-fat rice bran and defatted rice bran were studied in vitro and in vivo. Method Polysaccharides (GSRBPs) were extracted from Ganoderma sinense – full-fat rice bran (GS-FRB) and Ganoderma sinense – defatted rice bran (GS-DRB) fermentation products. The structure information of the GSRBPs was studied using HPLC analysis. The anti-tumor activities on H1299 NSCLC of GSRBPs in vitro study was performed using MTT method. The in vivo studies use BALB/c-nu nude mice as H1299 NSCLC bearing mice. Result All the polysaccharides contained two fractions, GSFPS-1 and GSFPS-2. The molecular weight and the ratio of GSFPS-1 and GSFPS-2 were different in GS-FRB and GS-DRB. At the earlier state of fermentation, all polysaccharides were composed of D-glu, D-man, D-xyl and L-ara with certain molar ratios. But at the latter stage, polysaccharides in GS-FRB were composed of D-glu, D-man, D-xyl, L-ara and D-fru, while these in GS-DRB only composed of D-glu and D-man. In the in vitro study, the IC50 of RBS and GSRBPs was as GS-DRB-11 (40.62 μg/mL), GS-FRB-9 (43.82 μg/mL), GS-DRB-7 (48.08 μg/mL), RBS (49.56 μg/mL), GS-DRB-9 (49.91 μg/mL), GS-DRB-13 (51.89 μg/mL), GS-FRB-11 (53.75 μg/mL), GS-FRB-7 (56.84 μg/mL), GS-DRB-13 (60.63 μg/mL) from small to large. In the in vivo study, the H1299 NSCLC inhibition rate (InRa) of RBS and GSRBPs were GS-DRB-11 (86.81%) > GS-DRB-9 (86.01%) > GS-FRB-9 (84.88%) > GS-DRB-7 (82.21%) > GS-DRB-13 (78.04%) > RBS (76.06%) > GS-FRB-13 (65.44%) > GS-FRB-11 (64.70%) > GS-FRB-7 (27.87%). The GSFPS-2 area percent was negatively correlated to the IC50 and was positively correlated to the InRa. This means the GSFPS-2 had much higher anti-tumor activity than GSFPS-1. Conclusion GSFPS-2 had higher anti-tumor activities, and the lipid in the rice bran has a decisive effect on the structures of polysaccharides produced by fermentation. Therefore, GSRBPs could be considered as potential novel agents to suppress H1299 non-small-cell lung cancer.

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Rice Bran By-Product: From Valorization Strategies to Nutritional Perspectives

Foods ◽

10.3390/foods10010085 ◽

2021 ◽

Vol 10 (1) ◽

pp. 85

Author(s):

Marco Spaggiari ◽

Chiara Dall’Asta ◽

Gianni Galaverna ◽

María Dolores del Castillo Bilbao

Keyword(s):

Rice Bran ◽

Food Industry ◽

Rice Grain ◽

Food Ingredient ◽

Grain Processing ◽

Health Related ◽

Physical Treatments ◽

Innovative Techniques

The aim of this study is to review the innovative techniques based on bioprocessing, thermal or physical treatments which have been proposed during the last few decades to convert rice bran into a valuable food ingredient. Rice bran (Oryza sativa) is the main by-product of rice grain processing. It is produced in large quantities worldwide and it contains a high amount of valuable nutrients and bioactive compounds with significant health-related properties. Despite that, its application in food industry is still scarce because of its sensitivity to oxidation processes, instability and poor technological suitability. Furthermore, the health-related effects of pretreated rice bran are also presented in this review, considering the up-to-date literature focused on both in vivo and in vitro studies. Moreover, in relation to this aspect, a brief description of rice bran arabinoxylans is provided. Finally, the application of rice bran in the food industry and the main technology aspects are concisely summarized.

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