scholarly journals Septal panniculitis as manifestation of COVID-19: own data

2021 ◽  
Vol 15 (4) ◽  
pp. 31-37
Author(s):  
O. N. Egorova ◽  
R. G. Goloeva ◽  
D. I. Abdulganieva
Keyword(s):  
Clinical Laboratory ◽  
Study Cohort ◽  
Adequate Treatment ◽  
Skin Changes ◽  
Persistent Symptoms ◽  
Medial Surfaces ◽  
Polymerase Chain ◽  
Severity Of The Disease ◽  
Subcutaneous Adipose ◽  
First Time

Objective: to study the clinical and laboratory features of erythema nodosum (EN) in a cohort of patients with COVID-19 referred to a rheumatological center.Patients and methods. During 2020–2021 years 21 patients (18 women and 3 men, mean age 43.2±11.4 years) with EN and polyarthralgia/arthritis were examined. Depending on the time of EN and articular syndrome associated with COVID-19 development, patients were divided into three groups: 1) up to 4 weeks – acute COVID (symptoms potentially associated with infection); 2) from 4 to 12 weeks – ongoing symptomatic COVID and 3) more than 12 weeks – post-COVID syndrome (persistent symptoms not associated with an alternative diagnosis). All patients underwent a comprehensive clinical, laboratory and instrumental examination, including ultrasound of the joints and chest computed tomography (CT), as well as pathomorphological examination of skin and subcutaneous adipose tissue from the site of the node (in 9 cases). Results and discussion. Based on the anamnesis data, COVID-19 in the study cohort had mild (in 13 patients) and moderate (in 8) severity. Two patients (21 years old and 23 years old) with mild severity of the disease noted red painful (45 mm on the visual analogue scale of pain) nodes on the legs and polyarthralgia for the first time on the 2nd – 3rd day from respiratory symptoms onset. In 9 (52.3%) patients, mainly with a mild course, similar skin changes were detected 24.5±7.6 days after active COVID-19 relieve, i.e. during the period of ongoing symptomatic COVID. In 8 (38%) patients, including 6 with moderate severity of the disease, the appearance of nodes was noted after 85.6±12.3 days, which corresponded to the post-COVID syndrome.At the time of examination, complaints of skin rashes and joint pain were reported in 100 and 71.4% of patients, respectively. 67% of patients had shortness of breath, weakness, cough, sweating and myalgia. Subfebrile fever had 5 (24%) patients, mainly with ongoing symptomatic COVID (3 patients). In the overwhelming majority of cases (86%), EN was located on the anterior and lateral surfaces of shins, less often on the posterior and medial surfaces. It is noteworthy that the affection of more than 50% of the surface of the lower and upper extremities was associated with the number of nodes (p<0.02), the level of CRP (p<0.03) and the presence of post-COVID syndrome (p<0.2). Fifteen (71.4%) patients had arthralgias, mainly of ankle (80%) and knee (53.3%) joints.Laboratory abnormalities included: median ESR was 34 [12; 49] mm/h, CRP level – 9 [2; 32] mg/l. The results of the polymerase chain reaction for SARS-CoV-2 were negative in all patients. In 100% of cases IgG antibodies to SARS-CoV-2 were detected and in 52.3% – IgM antibodies. On chest CT 5% lung affection was detected in 43% of patients, 5–25% lesion in 57.1% of patients, 8 (38%) of whom were with post-COVID syndrome. Pathomorphological examination of the nodes showed signs of septal panniculitis.Conclusion. When EN, associated with SARS-CoV-2 appears it is important to suspect a post-infectious manifestation in time, based on the clinical picture of the disease and to determine the scope of further examination and adequate treatment.

scholarly journals Management of Covid-19 Disease in Pediatric Oncology Patients

2021 ◽  
Author(s):  
Hatice Mine Cakmak
Keyword(s):  
Intensive Care Unit ◽  
Polymerase Chain Reaction ◽  
Clinical Laboratory ◽  
Pediatric Population ◽  
Single Agent ◽  
Radiological Features ◽  
Pediatric Cancer Patients ◽  
Polymerase Chain ◽  
Critical Patients ◽  
Severity Of The Disease

Pediatric cancer patients are immunocompromised, and the risks are higher in this population. Confirmed cases are defined as PCR (polymerase chain reaction) positive patients. The severity of infection is divided into four groups: asymptomatic/mild, moderate, severe, and critical, based on the clinical, laboratory, and radiological features. In the pediatric population, the COVID-19 disease has a mild course. Chemotherapy courses can be interrupted according to the symptoms and severity of the disease. Azithromycin, antivirals are used as a single agent or in combination. In critical patients, convalescent plasma, mesenchymal stem cells, tocilizumab, and granulocyte transfusions are administered. In recent studies, having hematological malignancy, stem cell transplantation, a mixed infection, and abnormal computerized tomography findings increase the severity of the disease and the need for an intensive care unit. Therefore, the patients and their families should be aware of a higher risk of severe forms than immunocompetent children.

scholarly journals Making a Difference: Adaptation of the Clinical Laboratory in Response to the Rapidly Evolving COVID-19 Pandemic

2021 ◽  
Vol 8 ◽  
pp. 237428952110239
Author(s):  
Nikhil S. Sahajpal ◽  
Ashis K. Mondal ◽  
Sudha Ananth ◽  
Allan Njau ◽  
Sadanand Fulzele ◽  
...  
Keyword(s):  
Clinical Laboratory ◽  
Clinical Manifestations ◽  
Nasopharyngeal Swab ◽  
Next Generation Sequencing Technology ◽  
Making A Difference ◽  
Scale Population ◽  
Wide Scale ◽  
Pooling Strategy ◽  
Polymerase Chain ◽  
Phylogenetic Evolution

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2, led to unprecedented demands assigned to clinical diagnostic laboratories worldwide, forcing them to make significant changes to their regular workflow as they adapted to new diagnostic tests and sample volumes. Herein, we summarize the modifications/adaptation the laboratory had to exercise to cope with rapidly evolving situations in the current pandemic. In the first phase of the pandemic, the laboratory validated 2 reverse transcription polymerase chain reaction–based assays to test ∼1000 samples/day and rapidly modified procedures and validated various preanalytical and analytical steps to overcome the supply chain constraints that would have otherwise derailed testing efforts. Further, the pooling strategy was validated for wide-scale population screening using nasopharyngeal swab samples and saliva samples. The translational research arm of the laboratory pursued several initiatives to understand the variable clinical manifestations that this virus presented in the population. The phylogenetic evolution of the virus was investigated using next-generation sequencing technology. The laboratory has initiated the formation of a consortium that includes groups investigating genomes at the level of large structural variants, using genome optical mapping via this collaborative global effort. This article summarizes our journey as the laboratory has sought to adapt and continue to positively contribute to the unprecedented demands and challenges of this rapidly evolving pandemic.

scholarly journals 440. Clinical Characteristics of Early Noncritical Hospitalized Patients with Coronavirus Disease 2019 (COVID-19): A Single-Center Retrospective Study in New York City

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S287-S288
Author(s):  
Michelle Lee ◽  
Mona Fayad ◽  
Tarub Mabud ◽  
Paulino Tallon de Lara ◽  
Adiac Espinosa Hernandez ◽  
...  
Keyword(s):  
New York ◽  
New York City ◽  
York City ◽  
Clinical Laboratory ◽  
Clinical Symptoms ◽  
New York State ◽  
General Medicine ◽  
Retrospective Chart Review ◽  
Clinical Severity ◽  
Study Cohort

Abstract Background COVID-19 first originated in Wuhan, China, in December 2019. As of April 9, 2020, New York State had become the single largest global epicenter of COVID-19. Methods This is a retrospective chart review of the first 33 patients with RT-PCR-confirmed COVID-19 admitted from the emergency department to a general medicine unit in a single academic hospital in New York City between March 11th to March 27th, 2020. Patient’s demographic, clinical, laboratory, radiographic investigations, treatments and clinical outcomes were retrospectively extracted from the electronic medical record and followed until April 10th, 2020. Patients were divided into severe and nonsevere sub-cohorts. Statistics were descriptive in nature. Results The study cohort (median age 68 yr, 67% male) presented with subjective fevers (82%), cough (88%), and dyspnea (76%). The median incubation period was 3 days. Most cases met SIRS criteria upon admission (76%). Patients had elevated inflammatory markers. Patients were treated with antimicrobials, corticosteroids, hydroxychloroquine, and varying levels of supplemental oxygen. Mortality was 15% and 18% of the cohort required intensive care services. Conclusion Patient age, presenting clinical symptoms, comorbidity profile, laboratory biomarkers, and radiographic features are consistent with findings published from China. Severe patients had peaks in inflammatory biomarkers later in the hospitalization, which may be useful to trend. Further studies are necessary to create guidelines to better risk-stratify COVID-19 patients based on clinical severity. Disclosures All Authors: No reported disclosures

FABP1 gene variant associated with risk of metabolic syndrome

2021 ◽  
Vol 24 ◽  
Author(s):  
Reza Zare-Feyzabadi ◽  
Majid Mozaffari ◽  
Majid Ghayour-Mobarhan ◽  
Mohsen Valizadeh
Keyword(s):  
Metabolic Syndrome ◽  
International Diabetes Federation ◽  
Amplification Refractory Mutation System ◽  
Study Cohort ◽  
Increased Risk ◽  
Mutation System ◽  
Polymerase Chain ◽  
The Relationship ◽  
Diabetes And Obesity

Background: Metabolic Syndrome (MetS) is defined by a clustering of metabolic abnormalities associated with an increased risk of cardiovascular disease and type 2 diabetes mellitus. There has been an increasing interest in the associations of genetic variants involved in diabetes and obesity in the FABP1 pathway. The relationship between the rs2241883 polymorphism of FABP1 and risk of MetS remains unclear. Objective: We aimed to examine the association between this genetic polymorphism and the presence of MetS and its constituent factors. Methods: A total of 942 participants were recruited as part of the Mashhad Stroke and Heart Atherosclerosis Disorders (MASHAD study) Cohort. Patients with MetS were identified using the International Diabetes Federation (IDF) criteria (n=406) and those without MetS (n=536) were also recruited. DNA was extracted from peripheral blood samples and used for genotyping of the FABP1 rs2241883T/C polymorphism using Tetra-Amplification Refractory Mutation System Polymerase Chain Reaction (Tetra-ARMS PCR). Genetic analysis was confirmed by gel electrophoresis and DNA sequencing. Results: Using both univariate and multivariate analyses after adjusting for age, sex and physical activity, carriers of C allele (CT/CC genotypes) in FABP1 variant were related to an increased risk of MetS, compared to non-carriers (OR: 1.38, 95%CI: 1.04,1.82, p=0.026). Conclusion: The present study shows that C allele in the FABP1 variant can be associated with an increased risk of MetS. The evaluation of these factors in a larger population may help further confirm these findings.

scholarly journals Rickettsia typhi IN RODENTS AND R. felis IN FLEAS IN YUCATÁN AS A POSSIBLE CAUSAL AGENT OF UNDEFINED FEBRILE CASES

2015 ◽  
Vol 57 (2) ◽  
pp. 129-132 ◽  
Author(s):  
Gaspar PENICHE-LARA ◽  
Karla DZUL-ROSADO ◽  
Carlos PÉREZ-OSORIO ◽  
Jorge ZAVALA-CASTRO
Keyword(s):  
Rattus Rattus ◽  
Conventional Polymerase Chain Reaction ◽  
Causal Agent ◽  
Wild Rodents ◽  
Murine Typhus ◽  
Rickettsia Typhi ◽  
Rickettsia Felis ◽  
Vector Borne ◽  
Polymerase Chain ◽  
First Time

Rickettsia typhi is the causal agent of murine typhus; a worldwide zoonotic and vector-borne infectious disease, commonly associated with the presence of domestic and wild rodents. Human cases of murine typhus in the state of Yucatán are frequent. However, there is no evidence of the presence of Rickettsia typhi in mammals or vectors in Yucatán. The presence of Rickettsia in rodents and their ectoparasites was evaluated in a small municipality of Yucatán using the conventional polymerase chain reaction technique and sequencing. The study only identified the presence of Rickettsia typhi in blood samples obtained from Rattus rattus and it reported, for the first time, the presence of R. felis in the flea Polygenis odiosus collected from Ototylomys phyllotis rodent. Additionally, Rickettsia felis was detected in the ectoparasite Ctenocephalides felis fleas parasitizing the wild rodent Peromyscus yucatanicus. This study’s results contributed to a better knowledge of Rickettsia epidemiology in Yucatán.

scholarly journals Detection of Toxoplasma gondii in a free-ranging giant anteater

2017 ◽  
Vol 47 (8) ◽  
Author(s):  
Thais Oliveira Morgado ◽  
Francielle Cristina Kagueyama ◽  
Janaina Marcela Assunção Rosa ◽  
Melissa Debesa Belizário ◽  
Richard de Campos Pacheco ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Agglutination Test ◽  
Zoonotic Infection ◽  
Chronic Infections ◽  
Chain Reaction ◽  
Positive Serology ◽  
Modified Agglutination Test ◽  
Polymerase Chain ◽  
Free Ranging ◽  
First Time

ABSTRACT: Toxoplasmosis is caused by Toxoplasma gondii, an obligatory intracellular protozoan, which establishes acute and chronic infections in birds and mammals, including humans. This note reports, for the first time, the detection and sequencing of DNA from T. gondii in the peripheral blood of a young free range giant anteater (Myrmecophaga tridactyla). For the diagnosis, the following methods were used: polymerase chain reaction (PCR) and positive serology (1:800) by means of the modified agglutination test (MAT). Since this species may be consumed by humans and predated by wild felids, its importance is emphasized as a probable source of zoonotic infection, in addition to its possible participation in the infection enzootic cycle. Although, parasitemia has been confirmed in this specimen, it presented no clinical sign of infection.

Agrobacterium-mediated insertional mutagenesis of the ochratoxigenic fungus Aspergillus westerdijkiae

2007 ◽  
Vol 53 (1) ◽  
pp. 148-151 ◽  
Author(s):  
Marcia M Mata ◽  
Marta H Taniwaki ◽  
Beatriz T Iamanaka ◽  
Daniele Sartori ◽  
André L.M Oliveira ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Insertional Mutagenesis ◽  
Aspergillus Ochraceus ◽  
Agrobacterium Mediated Transformation ◽  
Morphological Variations ◽  
Carcinogenic Potential ◽  
Coffee Beans ◽  
Aspergillus Westerdijkiae ◽  
Polymerase Chain ◽  
First Time

Aspergillus westerdijkiae is a potent ochratoxin A (OTA) producer that has been found in coffee beans. OTA is known to have nephrotoxic effects and carcinogenic potential in animal species. Here we report for the first time the Agrobacterium-mediated transformation for Aspergillus westerdijkiae and the generation of ochratoxin-defective mutants. Conidia were transformed to hygromycin B resistance using strain AGL-1 of Agrobacterium tumefaciens. The obtained transformation frequency was up to 47 transformants per 106 target conidia. Among 600 transformants, approximately 5% showed morphological variations. Eight transformants with consistently reduced OTA production were obtained. Two of these transformants did not produce OTA (detection limit: 0.1 µg/kg); the other six mutants produced lower amounts of OTA (1%–32%) compared with the wild-type strain. By using thermal asymmetric interlaced polymerase chain reaction, we successfully identified a putative flavin adenine dinucleotide monooxygenase gene.Key words: Aspergillus ochraceus, Aspergillus westerdijkiae, Agrobacterium-mediated transformation, Agrobacterium-mediated insertional mutagenesis, ochratoxin A.

Detection of a few DNA copies by real-time electrochemical polymerase chain reaction

The Analyst ◽  
2017 ◽  
Vol 142 (18) ◽  
pp. 3432-3440 ◽  
Author(s):  
M. Moreau ◽  
S. Delile ◽  
A. Sharma ◽  
C. Fave ◽  
A. Perrier ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Current Work ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
First Time ◽  
Accurate Quantification

In the current work, accurate quantification over 10 to 108 DNA copies has been successfully achieved for the first time by real-time electrochemical PCR.

scholarly journals Phylogenetic analysis of Black Bengal and Jamunapari goats in Bangladesh based on partial sequence of cytochrome b gene

2019 ◽  
Vol 17 (1) ◽  
pp. 23-35
Author(s):  
SMZH Chowdhury ◽  
MS Mahmud ◽  
MR Islam ◽  
KHMNH Nazir
Keyword(s):  
Cytochrome B ◽  
Gene Sequence ◽  
Yangtze River Delta ◽  
Climatic Conditions ◽  
Capra Hircus ◽  
Genetic Lineage ◽  
Phylogenetic Status ◽  
Polymerase Chain ◽  
First Time ◽  
Black Bengal Goat

Goats, among the livestock species, are considered the most prolific ruminant especially under callous climatic conditions. The aim of the present study was to depict the current phylogenetic status and genetic diversities of Black Bengal (BBG) and Jamunapari goat of Bangladesh and the world. Cytochrome b (cytb) gene (1140 bp) of mitochondrial DNA of Black Bengal goats (Capra hircus) was amplified by Polymerase Chain Reaction (PCR) for the first time in Bangladesh. The sequence from BBG had no nucleotide (nt) difference and 100% homology with the BBG (C. hircus) of India and also the goats (C. hircus) from China (Yangtze River Delta White Goat), Thailand (Wild Cervidae), Japan (Bezoar goat) and South Africa (Domestic goat). The sequence had 1-5 nt differences and 99% homology with the goats (C. hircus) from China, Thailand and Japan (other goats), and also with the goats (C. hircus) from Malaysia, South Korea, France, Italy, Pakistan, Slovenia, Switzerland and USA. Phylogenetic tree constructed with Black Bengal Goat (BBG-K-2) and Jamunapari goats (SG-1) of Bangladesh with cytochrome b nucleotide sequences were closely related to China-HM7. China-YP xj46, Pakistan-Lineage C1, Pakistan-Lineage C2, Slovenia- ChSo1, Switzerland-ChTo2992 and shared 98.8% to 99% and 98.3% to 98.6% similarity, respectively and 1-1.2% and 1.4 to 1.7% genetic distance, respectively. Based on Ctb gene Sequence collected from Bangladeshi Black Bengal Goats (BBG-K-2) and Jamunapari goats (SG- 1) that were closely related and shared with the same genetic lineage of China HM18 and India-BBG-DQ073048, respectively, suggesting a common origin. SAARC J. Agri., 17(1): 23-35 (2019)

scholarly journals Significance of Histopathology in Leprosy Patients with 1–5 Skin Lesions with Relevance to Therapy

2011 ◽  
Vol 3 (01) ◽  
pp. 021-024 ◽  
Author(s):  
S Veena ◽  
Prakash Kumar ◽  
Shashikala P. ◽  
Gurubasavaraj H. ◽  
H R Chandrasekhar ◽  
...  
Keyword(s):  
Drug Therapy ◽  
Skin Lesion ◽  
Skin Lesions ◽  
Clinicopathological Correlation ◽  
Adequate Treatment ◽  
Duration Of Therapy ◽  
Tuberculoid Leprosy ◽  
Leprosy Patients ◽  
Skin Biopsies ◽  
Severity Of The Disease

ABSTRACT Background: Patients with 1-5 skin lesions are clinically categorized as paucibacillary for treatment purposes. For betterment and adequate treatment of patients, this grouping needs further study. Aim: To study a group of leprosy patients with 1-5 skin lesions, compare clinical details with histopathological findings and bacteriological status of the skin to evaluate the relevance of this grouping. Materials and Methods: Two-year study involving 31 patients of leprosy with 1-5 skin lesions was included in this study. A number of skin lesions were recorded. Skin biopsies were taken in all patients. The biopsies were evaluated for the type of pathology and acid fast bacilli (AFB) status. Results: Of 31 patients, 19 (61.2%) had single skin lesion, 7 (22.5%) had two lesions, 4 (12.9%) had three lesions, and only one (3.22%) had four lesions, there were no patients with five lesions. Of the 31 patients, 30 (96.7%) were clinically diagnosed as borderline tuberculoid and one patient (3.22%) has tuberculoid leprosy. Skin smears were negative for AFB in all patients. The histological diagnoses were: TT 1 (3.22%), BT 24 (77.41%), and IL 6 (19.2%). AFB were found in 2 (6.45%) out of 31 skin biopsies. Clinicopathological correlation was 76.6% in the BT group. Conclusion: Tissue biopsy findings in 1-5 skin lesions which were not considered relevant for treatment purposes until now should be given a status in the categorization and assessment of severity of the disease. The significance of finding of AFB and histopathology of multibacillary (MB) type of leprosy in tissue biopsies, in patients grouped as PB should be resolved so that patients could be given the drug therapy and duration of therapy they warrant.

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