Artificial rubber mineralization by co-cultured bacterial strains

Objective: This study involves the use Natural Rubber degrading Bacterial co-cultures isolated from rubber plantation soil for the degradation of Artificial Rubber.Methods:Plate assay method and liquid assay method by using Mineral Salt Medium was followed for screening of bacteria for its capacity to mineralize Artificial rubber. Degradation was confirmed by Spectrophotometric and Fourier Transform Infra-Red (FTIR) studies. The co-cultures were able to utilize the artificial rubber which was confirmed by Spectrophotometric and FTIR studies.Results: Bacterial cocultures have the capacity to mineralize artificial rubber. Mineralization was observed for 30 days and it was maximum (6.48 x 10-5) on the 20th Day. This was confirmed using SEM and FTIR.Further, these were applied on bioplastics and plastics to study their capacity to degrade them. It was able to degrade them as well which was confirmed using FTIR.Conclusion: From current investigation, it can be concluded that the bacterial cocultures Bacillus cohnii and Brevundimonas naejangsanensis have the capacity to mineralize artificial rubber, plastics and bioplastics. Hence such isolated cocultures can be used in removal of artificial rubber, plastics and bioplastic wastes from the environment.

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Enrichment and Analysis of Stable 1,4-dioxane-Degrading Microbial Consortia Consisting of Novel Dioxane-Degraders

Microorganisms ◽

10.3390/microorganisms8010050 ◽

2019 ◽

Vol 8 (1) ◽

pp. 50 ◽

Cited By ~ 3

Author(s):

Tanmoy Roy Tusher ◽

Takuya Shimizu ◽

Chihiro Inoue ◽

Mei-Fang Chien

Keyword(s):

Ribosomal Rna ◽

Industrial Wastewater ◽

Microbial Consortium ◽

Mineral Salt Medium ◽

Intergenic Spacer ◽

Mineral Salt ◽

Microbial Consortia ◽

Salt Medium ◽

Bacterial Strains ◽

Water Contaminant

Biodegradation of 1,4-dioxane, a water contaminant of emerging concern, has drawn substantial attention over the last two decades. A number of dioxane-degraders have been identified, though many of them are unable to metabolically utilize 1,4-dioxane. Moreover, it is considered more preferable to use microbial consortia rather than the pure strains, especially in conventional bioreactors for industrial wastewater treatment. In the present study, a stable 1,4-dioxane-degrading microbial consortium was enriched, namely 112, from industrial wastewater by nitrate mineral salt medium (NMSM). The consortium 112 is capable of utilizing 1,4-dioxane as a sole carbon and energy source, and can completely degrade 1,4-dioxane up to 100 mg/L. From the consortium 112, two 1,4-dioxane-degrading bacterial strains were isolated and identified, in which the Variovorax sp. TS13 was found to be a novel 1,4-dioxane-degrader that can utilize 100 mg/L of 1,4-dioxane. The efficacy of the consortium 112 was increased significantly when we cultured the consortium with mineral salt medium (MSM). The new consortium, N112, could utilize 1,4-dioxane at a rate of 1.67 mg/L·h. The results of the ribosomal RNA intergenic spacer analysis (RISA) depicted that changes in the microbial community structure of consortium 112 was the reason behind the improved degradation efficiency of consortium N112, which was exhibited as a stable and effective microbial consortium with a high potential for bioremediation of the dioxane-impacted sites and contaminated industrial wastewater.

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Demineralization of Rajmahal Gondwana Coals by Bacteria: Revelations from X-Ray Diffraction (XRD) and Fourier Transform Infra Red (FTIR) Studies

Energy Exploration & Exploitation ◽

10.1260/0144-5987.33.5.755 ◽

2015 ◽

Vol 33 (5) ◽

pp. 755-767 ◽

Cited By ~ 22

Author(s):

Asha Lata Singh ◽

Prakash K Singh ◽

Aniruddha Kumar ◽

Mahendra P Singh

Keyword(s):

Fourier Transform ◽

X Ray Diffraction ◽

X Ray ◽

Ftir Studies ◽

Infra Red

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Mineralisation of natural rubber (poly cis 1-4 isoprene) by co-cultured bacterial strains isolated from rubber plantation area

International Journal of Biological Research ◽

10.14419/ijbr.v4i1.5552 ◽

2016 ◽

Vol 4 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

Manasa Muralidharan ◽

Veena Gayathri Krishnaswamy

Keyword(s):

Natural Rubber ◽

Enrichment Culture ◽

Nitrogen Sources ◽

Culture Technique ◽

Contaminated Site ◽

Assay Method ◽

Rubber Plantation ◽

Bacterial Strains ◽

16S R Rna ◽

Physico Chemical

Objective: To isolate Natural Rubber degrading Bacterial co-cultures from rubber plantation soil and to characterize and identify the organisms by 16s r RNA sequencing.Methods: Cocultures of the bacteria were isolated from the contaminated site by enrichment culture technique. Plate assay method and liquid assay method by using Mineral Salt Medium was followed for screening of bacteria for its capacity to mineralize Natural rubber. Degradation was confirmed by Spectrophotmetric and Fourier Transform Infra-Red (FTIR) studies.Natural rubber degraded by the cocultures were studied at different concentrations and the physico-chemical analysis were optimized (pH, temperature, carbon and nitrogen sources)Results: Isolated organism was identified as Bacillus cohnii and Brevundimonas naejangsanensis. The co-cultures were able to utilize the Natural rubber which was confirmed by Spectrophotometric and FTIR studies. From the current study it was evaluated that Natural rubber was mineralized up to 50 % where optimum concentration was 10 %.Conclusion: From current investigation, it can be concluded that our isolated bacterial cocultures Bacillus cohnii and Brevundimonas naejangsanensis have the capacity to mineralize Natural rubber and hence such isolated cocultures can be used in removal of from waste Natural rubber products in the environment.

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PEMBUATAN DAN KARAKTERISASI SERAT HOLLOW DARI SELULOSA BAKTERIAL DENGAN NANOPARTIKEL ZnO UNTUK PENGOLAHAN AIR LIMBAH TEKSTIL

Arena Tekstil ◽

10.31266/at.v29i1.849 ◽

2014 ◽

Vol 29 (1) ◽

Author(s):

Rizka Yulina ◽

Srie Gustiani ◽

Wulan Septiani

Keyword(s):

Fourier Transform ◽

Acetobacter Xylinum ◽

Wet Spinning ◽

Ph Optimum ◽

Infra Red ◽

Remazol Black

Selulosa bakterial mempunyai sifat mekanik yang baik untuk digunakan sebagai membran pada proses pengolahan air limbah tekstil. Pada penelitian ini dilakukan pembuatan dan karakterisasi serat hollow dari bahan baku selulosa bakterial nata de coco dengan penambahan nanopartikel ZnO sebagai fotokatalis terimmobilisasi. Selulosa bakterial disintesis menggunakan bakteri Acetobacter xylinum di dalam medium air kelapa dan gula. Selulosa bakterial dilarutkan bersama dengan nanopartikel ZnO menggunakan pelarut cuprietilen diamina (Cuen) dengan variasi selulosa bakterial 2,25% dan 2,50%. Serat berbentuk hollow dihasilkan dari proses wet spinning menggunakan koagulan NaOH. Serat hollow yang telah melalui koagulan kemudian direndam dalam larutan asam, gliserol, dan alkohol, dengan variasi waktu perendaman asam selama 1 dan 2 hari. Uji kekuatan tarik menunjukkan hasil yang terbaik yakni sebesar 815,72 gf pada konsentrasi selulosa bakterial 2,50% dan perendaman asam selama 2 hari. Dari hasil uji gugus fungsi menggunakan spektroskopi Fourier Transform Infra Red (FTIR), terdapat beberapa gugus fungsi yang menunjukkan keberadaan selulosa dan nanopartikel ZnO. Proses dekolorisasi fotokatalitik terhadap air limbah tekstil artifisial yang mengandung zat warna reaktif Remazol Black 5 (RB5) menunjukkan bahwa pH optimum proses penyisihan warna yakni pada pH 9 dan dihasilkan persen penyisihan warna yang tertinggi yaitu 90,32%. Pada kondisi yang sama, proses dekolorisasi RB5 menggunakan serat hollow tanpa nanopatikel ZnO hanya menghasilkan persen penyisihan warna sebesar 32,10%. Berdasarkan laju penyisihan zat warna, aktivitas degradasi fotokatalitik terbesar (k’ = 0,2615) diperoleh pada konsentrasi ZnO 10% dan konsentrasi zat warna RB5 10 ppm.

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PENGGUNAAN TiO2 PARTIKEL NANO HASIL SINTESIS BERBASIS AIR MENGGUNAKAN METODA SOL-GEL PADA BAHAN KAPAS SEBAGAI APLIKASI UNTUK TEKSTIL ANTI UV

Arena Tekstil ◽

10.31266/at.v28i1.1434 ◽

2013 ◽

Vol 28 (1) ◽

Author(s):

Maya Komalasari ◽

Bambang Sunendar

Keyword(s):

Fourier Transform ◽

Electron Microscope ◽

Scanning Electron Microscope ◽

Sol Gel ◽

Crosslinking Agent ◽

X Ray Diffraction ◽

Nano Tio2 ◽

X Ray ◽

Infra Red ◽

Scanning Electron

Partikel nano TiO2 berbasis air dengan pH basa telah berhasil disintesis dengan menggunakan metode sol-gel dan diimobilisasi pada kain kapas dengan menggunakan kitosan sebagai zat pengikat silang. Sintesis dilakukan dengan prekursor TiCl4 pada konsentrasi 0,3 M, 0,5 M dan 1 M, dan menggunakan templat kanji dengan proses kalsinasi pada suhu 500˚C selama 2 jam. Partikel nano TiO2 diaplikasikan ke kain kapas dengan metoda pad-dry-cure dan menggunakan kitosan sebagai crosslinking agent. Berdasarkan hasil Scanning Electron Microscope (SEM),diketahui bahwa morfologi partikel TiO2 berbentuk spherical dengan ukuran nano (kurang dari 100 nm). Karakterisasi X-Ray Diffraction (XRD) menunjukkan adanya tiga tipe struktur kristal utama, yaitu (100), (101) dan (102) dengan fasa kristal yang terbentuk adalah anatase dan rutile. Pada karakterisasi menggunakan SEM terhadap serbuk dari TiO2 yang telah diaplikasikan ke permukaan kain kapas, terlihat adanya imobilisasi partikel nano TiO2 melalui ikatan hidrogen silang dengan kitosan pada kain kapas. Hasil analisa tersebut kemudian dikonfirmasi dengan FTIR (Fourier Transform Infra Red) yang hasilnya memperlihatkan puncak serapan pada bilangan gelombang 3495 cm-1, 2546 cm-1, dan 511 cm-1, yang masing-masing diasumsikan sebagai adanya vibrasi gugus fungsi O-H, N-H dan Ti-O-Ti. Hasil SEM menunjukkan pula bahwa kristal nano yang terbentuk diantaranya adalah fasa rutile , yang berdasarkan literatur terbukti dapatberfungsi sebagai anti UV.

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