Adsorption of Malachite Green (MG) on activated carbon obtained from Teak wood biomass has been investigated by batch adsorption method. The percentage removal of dye has been optimized by studying the initial concentration of the dye, adsorbent dosage, adsorption time and pH. The experimental data were found to be well fitted to Langmuir isotherm. The present study indicates that activated carbon obtained from Teak wood biomass is an effective adsorbent of MG dye. The prepared activated carbon was evaluated for antimicrobial activity against (Staphylococcus aureus, Escherichia coli and Aspergillus flavus, Aspergillus niger), using disc diffusion method and compared with standard antibacterial (Nitrofurantoin) and antifungal (Amphotericin B). Bacteria showing more activity than fungal strains.