scholarly journals Genetic Diversity and Demography of Skipjack Tuna (Katsuwonus pelamis) In Southern and Western Part of Indonesian Waters

2019 ◽  
Vol 24 (2) ◽  
pp. 61
Author(s):  
Irwan Jatmiko ◽  
Raymon Rahmanov Zedta ◽  
Maya Agustina ◽  
Bram Setyadji
Keyword(s):  
Genetic Diversity ◽  
Fisheries Management ◽  
Pcr Amplification ◽  
Skipjack Tuna ◽  
Migratory Species ◽  
Katsuwonus Pelamis ◽  
Tissue Samples ◽  
Management Area ◽  
Polymerase Chain ◽  
Group 2

Skipjack tuna (Katsuwonus pelamis) is highly migratory species that spread from trophic and sub trophic waters. This species can be found in Atlantic, Indian and Pacific oceans. The genetic information of highly migratory species like skipjack tuna is important to support the sustainability of the fisheries. The objectives of this study are to gain information genetic diversity and population structure of exploited species and to understand the population kinship in Indonesian waters. Tissue samples were collected from six locations, i.e.: Sibolga (North Sumatera), Padang (West Sumatera), Binuangeun (Banten), Pacitan (East Java), Lombok (West Nusa Tenggara) and Kupang (East Nusa Tenggara). Microsatellite analysis was done in this study consisting of extraction, purification, polymerase chain reaction (PCR) amplification and electrophoresis. Three loci used for the analysis i.e.: UTD 172, UTD 523 and UTD 535. The results showed that there are two groups from six locations i.e.: group 1: Sibolga and Padang; group 2: Binuangeun, Pacitan Lombok and Kupang. The variance among these two groups is 0.066 with variance 5.441%. This finding in line with Indonesian Fisheries Management Area of 572 (west of Sumatera waters) and 573 (south of Java waters). However, as highly migratory species across nations, the management strategy for skipjack tuna needs collaboration among countries through regional fisheries management authority like Indian Ocean Tuna Commission (IOTC).

scholarly journals Keragaman Genetik Ikan Cakalang (Katsuwonus pelamis) di Perairan Laut Maluku Utara

DEPIK ◽  
2018 ◽  
Vol 7 (2) ◽  
pp. 164-176
Author(s):  
Nebuchad Nezzar Akbar ◽  
Rusmawati Labenua
Keyword(s):  
Genetic Diversity ◽  
Fisheries Management ◽  
Negative Impact ◽  
Genetic Data ◽  
Molecular Characteristics ◽  
Skipjack Tuna ◽  
Migratory Species ◽  
Sustainable Fisheries ◽  
Katsuwonus Pelamis ◽  
The North

Skipjack tuna is a migratory species that migrate globally throughout the ocean. The North Maluku Sea has a big potency on skipjack, this resource has been exploited intensively and gave the negative impact on skipjack population. The genetic data is one of the important information to strategy a better sustainable fisheries management. Hence, the aims of the study were to analyze the genetic diversity data in North Maluku Sea. The samples were collected from three locations, namely Morotai Island, South Halamera and Bacan. A total of 10 samples were collected from every location. The samples were extracted with Chelex 10%, and processing for PCR and sequencing.  The results showed that molecular characteristics in skipjack are 546 (bp). The genetic diversity was ranged from 0.800-1.000 with the total of 28 haplotypes of 30 samples. The genetic diversity is included in high/undisturbed criteria and showed the population still in stable condition.Ikan Cakalang merupakan migratori  spesies yang bermigrasi secara global di seluruh samudera. Potensi ikan cakalang sangat tinggi di perairan laut Maluku Utara. Pemanfaatan sumberdaya ikan cakalang sudah dilakukan lama dan terus menurus sehingga berdampak pada penurunan, oleh karena itu perlu segera dilakukan pengelolaan secara berkelanjutan. Data genetik merupakan salah satu informasi penting untuk menyusun rencana pengelolaannya di perairan laut Maluku Utara. Oleh karena itu penelitian ini bertujuan untuk melakukan analisis keragamana genetik ikan cakalang di perairan Maluku Utara. Sampel ikan diperoleh dari tiga lokasi, yaitu Pulau Moratai, Halamera Selatan dan Bacan, masing-masing lokasi dengan 10 sampel. Hasil penelitian diperoleh karakteristik molekuler pada ikan cakalang 546 (bp). Keragaman genetik ditemukan berkisar 0.800-1.000 dengan jumlah 28 jaringan haplotipe dari 30 sampel. Nilai keragaman genetik masuk dalam kriteria tinggi/baik, sehingga bisa dikatakan bahwa populasi ikan masih dalam kondisi stabil. 

scholarly journals ESTIMASI PARAMETER POPULASI IKAN CAKALANG (Katsuwonus pelamis, Linnaeus, 1758) DI PERAIRAN SAMUDRA HINDIA

2018 ◽  
Vol 9 (3) ◽  
pp. 163
Author(s):  
Raymon Rahmanov Zedta ◽  
Prawira Atmaja Rintar PT ◽  
Dian Novianto Novianto
Keyword(s):  
Fisheries Management ◽  
Growth Parameters ◽  
Total Mortality ◽  
Skipjack Tuna ◽  
Population Parameters ◽  
Katsuwonus Pelamis ◽  
K Value ◽  
Fish Samples ◽  
Management Area ◽  
Tuna Fisheries

Penelitian ini bertujuan untuk mengkaji parameter populasi ikan cakalang (Katsuwonus pelamis) yang tertangkap pukat cincin di WPP-572 Samudra Hindia Barat Sumatera dan WPP-573 Selatan Jawa. Hasil penelitian ini diharapkan dapat digunakan sebagai masukan dalam penentuan pengelolaan perikanan sehingga stok ikan cakalang dapat dimanfaatkan secara berkelanjutan. Ikan contoh dikumpulkan melalui program enumerasi tahun 2016 (Januari-Desember). Sampling acak dilaksanakan di empat pelabuhan, yaitu PPS Lampulo (Aceh), PPN Sibolga (Sumatra Utara), PPP Tamperan (Pacitan) dan IPP Pondokdadap (Malang). Jumlah ikan contoh diperoleh sebanyak 14.894 ekor. Serial data fekuensi panjang bulanan diolah menggunakan program FiSAT II untuk menduga parameter pertumbuhan, mortalitas dan tingkat eksploitasi. Hasil penelitian menunjukkan ikan cakalang yang tertangkap di WPP-572 memiliki panjang asimtotik (L)73,5 cmFL, K sebesar 0.22/tahun dan to sebesar -0,59 tahun. Parameter populasi di WPP-573 berturut-turut L”=67,20 cmFL, K=0,27/tahun, dan to=-0,50 tahun. Nilai mortalitas alami (M) ikan cakalang di WPP 572 sebesar 0,49/tahun, mortalitas total (Z) 0,70/tahun, dan kematian akibat penangkapan (F) adalah 0,21/tahun. Ikan cakalang yang tertangkap di WPP 573 menunjukkan nilai (E) sebesar 0,59/tahun, nilai Z 1,02/tahun, dan nilai F sebesar 0,43/tahun. Dugaan tingkat eksploitasi ikan cakalang di WPP 572 dan 573 masing-masing 0,3/tahun dan 0,42/tahun atau belum berada pada tahap optimal.This study aimed to assess the population parameters of skipjack tuna (Katsuwonus pelamis) that caught by purse seine in the Indonesian FMA (Fisheries Management Area) 572 Indian Ocean West Sumatera and FMA 573 South Java. The outcomes of this research can hopefully be used as inputs for fisheries management, in order the stock of skipjack tuna can be utilized sustainably. Fish sample were collected through the program enumeration of Research Institute for Tuna Fisheries (RITF) during the year 2016 (Januari-Desember). Using random sampling method at four fishing ports, namely PPS Lampulo (Aceh), PPN Sibolga (North Sumatra), PPP Tamperan (Pacitan), and IPP Pondokdadap (Malang). The total number of fish samples was 14.894 fish. Monthly length frequency data processed using FiSAT II program to estimate the growth parameters, mortality, and exploitation. The analysis results showed that skipjack tuna caught in FMA 572 has asymtotic length value (L) at 73.5 cmFL, K value 0.22/year, and to at -0.59 year; while in FMA 573 population parameters values respectively 67.20 cmFL, 0.27/year, and -0.50 year. The value of natural mortality (M) skipjack in FMA 572 is 0.49/year, total mortality (Z) 0.70/year, and fishing mortality (F) 0.21/year. Skipjack tuna that caught in FMA 573 showed value of M 0.59/year, Z value 1.02/year, and F value 0.43/year. The estimated values of exploitation levels of skipjack in FMA 572 and 573 were 0.3/year and 0.42/year respectively.

scholarly journals Genetic Diversity in Ficus Sycomorus L. Species (Moraceae) Using Rapd and Irap Markers

2013 ◽  
Vol 59 (3) ◽  
pp. 120-130 ◽  
Author(s):  
Basel Saleh
Keyword(s):  
Genetic Diversity ◽  
Pcr Amplification ◽  
Coastal Regions ◽  
Chain Reaction ◽  
Marker Index ◽  
Irap Markers ◽  
Irap Marker ◽  
Repeatability And Reproducibility ◽  
Ficus Sycomorus ◽  
Polymerase Chain

Abstract This study was conducted in order to assess accuracy, repeatability and reproducibility of the RAPD and IRAP techniques for determining the genetic variability in 10 Ficus sycomorus L. genotypes grown in the coastal regions of Syria. Thirty-six RAPD primers applied gave 352 discernible loci, of which 252 (71.59%) were polymorphic. Polymerase chain reaction (PCR) amplification with 36 RAPD primers gave an average of 9.778 selected markers/primers, with a maximum of 21 (OPA18) and a minimum of five (OPG11, OPK12 and OPT18). The amplification with 22 IRAP primers (single or combination) generated 178 bands, of which 151 (84.83%) were polymorphic, with an average of 11.125 selected markers/ primer, with a maximum of 17 (IRAP-TDK11F) and a minimum of seven (BREP1F+BREP1R, IRAP-TDK1F+IRAP- TDK1R and IRAP-TDK2F+IRAP-TDK2R). In the present investigation, the IRAP marker was more efficient than the RAPD assay, where the latter exhibited a lower marker index (MI) average (1.629) compared with the IRAP technique (2.941). Otherwise, F. sycomor4 genotype showed the highest dissimilarity compared with other genotypes studied in this investigation. Based upon the estimated percent disagreement values (PDV), we can suggest that there are three subspecies present among the 10 samples tested.

scholarly journals Skipjack Tuna Fishing Ground Based on Oceanography Satellite Image Data in Fisheries Management Area (FMA) 713

2020 ◽  
pp. 51-60
Author(s):  
. Safruddin ◽  
Rachmat Hidayat ◽  
Mukti Zainuddin
Keyword(s):  
Chlorophyll A ◽  
Fisheries Management ◽  
Satellite Image ◽  
Image Data ◽  
Sea Surface ◽  
Fishing Ground ◽  
Skipjack Tuna ◽  
Management Area ◽  
Tuna Fishing ◽  
Satellite Image Data

This study aimed to map the oceanographic conditions and, based on that, to determine the favorable condition of skipjack tuna fishing ground in Fisheries Management Area (FMA) 713. This condition includes sea surface temperature (SST) and chlorophyll-a concentration. The primary data, such as fishing ground position and fish catch per trip, was collected by following the fishing operations that utilized pole and line (in Bone Gulf and Flores Sea) and purse seine fishing operations (in Makassar Strait). The secondary data, such as SST and chlorophyll-a, was derived from oceanographic satellite image data. The result showed that from January to August 2019, the skipjack tuna tends to occupy off shore areas where the sea surface temperature is between 29 - 29.5 oC and the chlorophyll-a density is between 0.10 to 0.15 mg.m3.

scholarly journals Assessment of genetic structure and diversity of Erodium (Geranaiceae) species

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 507-520
Author(s):  
Lejing Lin ◽  
Li Lin ◽  
Abdul Waheed
Keyword(s):  
Genetic Diversity ◽  
Isolation By Distance ◽  
Pcr Amplification ◽  
Molecular Data ◽  
Mantel Test ◽  
Test Results ◽  
Species Characteristics ◽  
Polymerase Chain ◽  
Morphological And Molecular Data ◽  
Genetic Structure And Diversity

Erodium Aiton (Geraniaceae) with 75 species is distributed in all continents except Antarctic. Its main diversification center is Mediterranean region with 62 species. The genetic diversity was assessed through Sequence-related amplified polymorphism. To uncover genetic diversity and species characteristics in Erodium species, were studied through a combination of morphological and molecular data. 70 individuals related to seven Erodium were collected in 7 provinces. A total of 96 (Number of total loci) (NTL) DNA bands were produced through polymerase chain reaction amplifications (PCR) amplification of seven Erodium species. These bands were produced with the combinations of six selective primers. The total number of amplified fragments ranged from 10 to 25. The genetic similarities between seven species are estimated from 0.70 to 0.85. Clustering results showed two major clusters. This study also detected a significant signature of isolation by distance (Mantel test results). Present results showed that sequence-related amplified polymorphism have the potential to identify and decipher genetic affinity in Erodium species. Current results have implications in biodiversity and conservation programs. Besides this, present results could pave the way for selecting suitable ecotypes for forage and pasture purposes in Iran.

scholarly journals Pemanfaatan Data Biologi Ikan Cakalang (Katsuwonus pelamis) dalam Rangka Pengelolaan Perikanan Bertanggung Jawab di Perairan Teluk Bone

2012 ◽  
Vol 14 (1) ◽  
pp. 107 ◽  
Author(s):  
Muhammad Jamal ◽  
Muhammad Fedi Alfiadi Sondita ◽  
John Haluan ◽  
Budy Wiryawan
Keyword(s):  
Growth Model ◽  
Fisheries Management ◽  
Growth Pattern ◽  
Fork Length ◽  
Biological Characteristics ◽  
Skipjack Tuna ◽  
Katsuwonus Pelamis ◽  
Management Zone ◽  
Weight Relationship ◽  
First Maturity

The Bone Bay is part of Fisheries Management Zone WPPI 713 that covers Makassar strait, Flores sea and Bali sea. To such vast marinearea, fisheries management can be started from smaller region, e.g. embayment waters. Biological characteristics of skipjack tuna (Katsuwonuspelamis) are some of important information for developing fisheries management in the area. This research was aimed to determine length-weight relationship, size composititon, growth pattern and biologically feasible size of the fish. Data were obtained from fish caught byfisherman from Januari to December 2007. The bay was divided into three study areas, i.e. Northern, Centre and Southern Zones. The bcoefficients for length-weight relationship in the three zones were equal 3 [not significant]. The average fork-length (FL) in each zoneshowed similar trends: increased from January to June, then stabilized until December. Using von Bertalanffy’s growth model, the maximumFL (L ∞ of 759.75 mm is estimated for fish at age of 84 months. The length at first maturity of the fish is 465 mm (FL).

scholarly journals Detection of a putative novel adenovirus by PCR amplification, sequencing and phylogenetic characterisation of two gene fragments from formalin-fixed paraffin-embedded tissues of a cat diagnosed with disseminated adenovirus disease

2017 ◽  
Vol 65 (4) ◽  
pp. 574-584 ◽  
Author(s):  
Béla Lakatos ◽  
Ákos Hornyák ◽  
Zoltán Demeter ◽  
Petra Forgách ◽  
Frances Kennedy ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Polymerase ◽  
Pcr Amplification ◽  
Adenovirus Infection ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Polymerase Chain ◽  
Formalin Fixed

Adenoviral nucleic acid was detected by polymerase chain reaction (PCR) in formalin-fixed paraffin-embedded tissue samples of a cat that had suffered from disseminated adenovirus infection. The identity of the amplified products from the hexon and DNA-dependent DNA polymerase genes was confirmed by DNA sequencing. The sequences were clearly distinguishable from corresponding hexon and polymerase sequences of other mastadenoviruses, including human adenoviruses. These results suggest the possible existence of a distinct feline adenovirus.

scholarly journals STATUS KEBERLANJUTAN KETERSEDIAAN SUMBERDAYA PERIKANAN CAKALANG (Katsuwonus pelamis) DI PERAIRAN ACEH BARAT

2015 ◽  
Vol 2 (2) ◽  
Author(s):  
Edwarsyah Edwarsyah ◽  
Mohamad Gazali
Keyword(s):  
Multidimensional Scaling ◽  
Fisheries Management ◽  
Local Community ◽  
Skipjack Tuna ◽  
Katsuwonus Pelamis ◽  
Sustainability Index ◽  
Fisheries Resources ◽  
Fishing Gears ◽  
The Status ◽  
Index Value

Fisheries production in West of Aceh was still insufficient to provide the local community consumption. However, fisheries resources in  West of Aceh water  are abundant. But utilization of fisheries particularly skipjack tuna (cakalang) hasn’t been yet optimalized. This aiming to analyse the status of sustainablity of skipjack (Katsuwonus pelamis) fisheries in West of Aceh water.  The study was conducted in November to Desember 2012 at the Fish Landing  Port of  Kuala Bubon  and Fish Landing Bases (PPI) Ujong Baroh. The result shown that sustainability index value by using multidimensional scaling is 88,42 with stress value is  0.12 and coefisien of determination (R2) is 0,95 or 95%. Such index value show that sustainablity of skipjack resources were good status. It means that multidimensional of skipjack stock were still paid attention in fisheries management sustainable. Beside that, it shown from water condition and fishing gears were still support viability of such comodity.

scholarly journals Genetic Diversity and Emerging Zoonotic Onchocerca Species in Human Populations in Taraba State, Nigeria

2021 ◽  
Author(s):  
Danlami Akafyi ◽  
Iliya Shehu Ndams ◽  
Ishaya Haruna Nock ◽  
Gloria Chechet ◽  
Renz Alfons ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Pcr Amplification ◽  
Human Populations ◽  
Rrna Gene ◽  
Species Identity ◽  
Multiple Sequence ◽  
Centrifuge Tube ◽  
Polymerase Chain ◽  
Endemic Areas ◽  
Skin Snips

Abstract Background: A better understanding of parasite population genetic processes in specific biogeography is needed to support onchocerciasis elimination goals. The genetic diversity of Onchocerca microfilariae was explored by amplifying a fragment of the 16S rRNA gene in the endemic area in Taraba State, Nigeria, Methods: Eight (8) communities were selected including six onchocerciasis endemic communities with records of ivermectin treatment having been annually distributed for 10 to 16 years, and two non-onchocerciasis endemic areas. The participants were 211 from endemic and 110 from non-endemic areas as control. Skin snips were taken from consenting participants by convenience sampling methods using a sterile sclera punch, from males and females residing within the communities for ten years and above or since birth, microfilaria and residual skin snips were preserved in RNALater® in a 1.5 ml micro-centrifuge tube. DNA was extracted from microfilariae recovered and from those in residual skin snip specimens. Polymerase Chain Reaction (PCR) amplification using specific primers for 16S genes was done to detect the identity of Onchocerca species. The amplified products were sequenced and analyzed for species identity. Results: Multiple sequence alignment and phylogenetic analysis results showed distinct diversity of two sample sequences (G49_O.v. Gashaka and Y02_O.v. Yorro) from other samples from the study area and other regions, indicating emergence of a new polymorphic strain of O. volvulus. Report of a preliminary case of emerging zoonosis of O. ochengi infection in human (skin snips) sample (O. ochengi G44) in this study. Conclusions: It is clear there is genetic diversity of Onchocerca species and emerging zoonosis in the study site. We suggest further investigation on the extent and potentials of emerging zoonotic onchocerciasis by O. ochengi, in the light of cattle, Simulium vectors, environmental and humans overlap in the study area.

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