scholarly journals Seroprevalence and associated risk factors of avian influenza virus subtype H9N2 in backyard poultry of Peshawar Pakistan

2021 ◽  
Vol 46 (3) ◽  
pp. 209-218
Author(s):  
S. Rehman ◽  
M. I. Khan ◽  
F. A. Rantam ◽  
M. H. Effendi ◽  
A. Shehzad ◽  
...  
Keyword(s):  
Risk Factors ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Respiratory Infections ◽  
Haemagglutination Inhibition ◽  
Influenza Viruses ◽  
Chi Square ◽  
Backyard Poultry ◽  
Associated Risk Factors

The current study was conducted to estimate the serological prevalence and risk factors associated with the transmission of avian influenza subtype H9N2 among backyard poultry from different villages of semi-arid climate district Peshawar Pakistan between January to May 2019. In total, 240 blood samples of healthy backyard poultry older than two months were collected from 30 different villages of district Peshawar. A predesigned questionnaire was used to collect the data related to risk factors. Se-rum samples were tested through the haemagglutination inhibition (HI) test further confirmed by the micro-neutralizing test (MN). Antibody body titer ≤ 8 considered negative for prevalence of H9N2. In order to classify risk factors, Chi-square and Logistic regression analyses were performed. Out of 240, 150 were found to be positive. The overall seroprevalence was 62.5%, mean antibody titer for avian influenza virus in all villages was 6.8 and (95%) confidence interval ranges from 35.33 to 51.70% re-spectively. The high prevalence of antibodies to avian influenza viruses in bird serum emphasizes that avian influenza plays an important role in the respiratory infections of backyard chickens in the area, and possibly throughout Pakistan. In order to avoid the introduction of H9N2, biosecurity initiatives, surveillance, monitoring systems, and to some extent, vaccinations are useful tools.

scholarly journals Characterization of a Low Pathogenic Avian Influenza Virus (H6N1) Isolated from Turkeys

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
I. Shkoda ◽  
E. Lapin ◽  
E. Rosenbluth ◽  
S. Perk ◽  
Z. Geva ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Haemagglutination Inhibition ◽  
Influenza Viruses ◽  
Specific Primers ◽  
Domestic Ducks ◽  
Turkey Poults ◽  
Pathogenic Avian Influenza Virus

An avian influenza virus (AIV), A/turkey/Israel/09 subtype H6N1, was isolated from turkey poults exhibiting typical pathology associated with AIV infection. The virus was characterized by RT-PCR using AIV subtype-specific primers and by the haemagglutination inhibition test using AIV subtype-specific antisera. The virus has an intravenous pathogenicity index of 0 and possessed a nucleotide sequence at the cleavage site of the hemagglutinin gene, PQIETR*GLF, associated with avian influenza viruses of low pathogenicity. Unlike the two previous H6N2 isolates originating from domestic ducks and mallard, the A/turkey/Israel/09 (H6N1) was isolated from turkeys. The gene sequences of the A/turkey/Israel/09 (H6N1) virus show divergence from the former Israeli H6 isolates.

scholarly journals Serosurvey of Avian Influenza Viruses (H5, H7, and H9) and Associated Risk Factors in Backyard Poultry Flocks of Lahore District, Pakistan

2021 ◽  
Vol 8 ◽  
Author(s):  
Mamoona Chaudhry ◽  
Hamad Bin Rashid ◽  
Michael Thrusfield ◽  
Mark C. Eisler ◽  
Susan C. Welburn
Keyword(s):  
Risk Factors ◽  
Avian Influenza ◽  
Influenza Viruses ◽  
Flock Size ◽  
Avian Influenza Viruses ◽  
Cross Sectional Survey ◽  
Backyard Poultry ◽  
Associated Risk Factors ◽  
Backyard Birds ◽  
Different Sources

Rural poultry constitutes 56% of the total poultry population in Pakistan; however, epidemiological information about avian influenza viruses (AIVs) in backyard poultry flocks is lacking. A cross-sectional survey of villages of Lahore district was conducted from July 2009 to August 2009 using two-stage cluster sampling and probability proportional to size (PPS) sampling to estimate seroprevalence and its associated risk factors. A random selection of 35 clusters from 308 villages of Lahore were considered, and from each cluster, six chickens aged >2 months were selected. A total of 210 serum samples were collected and examined by the hemagglutination inhibition (HI) test for specific antibodies against AIV subtypes H5, H7, and H9. Overall weighted seroprevalence for AIVs was 65.2% (95% CI: 55.6–74.8%), and for subtype H5, H7 & H9 was 6.9% (95% CI: 10.8–23.0%), 0% (95% CI: 0–1.7%), and 62.0% (95% CI: 52.2–71.8%) respectively. However, none of the samples were positive for H7. The average flock size was 17.3 birds, and the main purpose of keeping poultry was for eggs/meat (70.6%, 95% CI: 59.7–81.4). A majority of them were reared in a semi-caged system (83%, 95% CI: 74.5–91.3). Backyard birds were received from different sources, that is, purchased from the market or received as a gift from friends or any NGO, and were 5.7 times more likely to become avian influenza (AI) seropositive than those that were not exposed to these sources (CI 95%: 2.0–716.0). Backyard birds which were received from different sources, that is, purchased from the market or received from friends or any NGO, were 5.7 times more likely to become AI seropositive compared to those that were not (CI 95%: 2.5–18.7). To reduce the risk of AIV in Pakistan, continuous surveillance of backyard poultry would be needed.

scholarly journals Putative human and avian risk factors for avian influenza virus infections in backyard poultry in Egypt

2014 ◽  
Vol 168 (1) ◽  
pp. 208-213 ◽  
Author(s):  
Basma M. Sheta ◽  
Trevon L. Fuller ◽  
Brenda Larison ◽  
Kevin Y. Njabo ◽  
Ahmed Samy Ahmed ◽  
...  
Keyword(s):  
Risk Factors ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Virus Infections ◽  
Backyard Poultry

scholarly journals Swine ANP32A Supports Avian Influenza Virus Polymerase

2020 ◽  
Vol 94 (12) ◽  
Author(s):  
Thomas P. Peacock ◽  
Olivia C. Swann ◽  
Hamish A. Salvesen ◽  
Ecco Staller ◽  
P. Brian Leung ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Virus Replication ◽  
Mammalian Species ◽  
Gene Mutations ◽  
Influenza Viruses ◽  
Avian Influenza Viruses ◽  
2009 H1n1 ◽  
Mixing Vessels

ABSTRACT Avian influenza viruses occasionally infect and adapt to mammals, including humans. Swine are often described as “mixing vessels,” being susceptible to both avian- and human-origin viruses, which allows the emergence of novel reassortants, such as the precursor to the 2009 H1N1 pandemic. ANP32 proteins are host factors that act as influenza virus polymerase cofactors. In this study, we describe how swine ANP32A, uniquely among the mammalian ANP32 proteins tested, supports the activity of avian-origin influenza virus polymerases and avian influenza virus replication. We further show that after the swine-origin influenza virus emerged in humans and caused the 2009 pandemic, it evolved polymerase gene mutations that enabled it to more efficiently use human ANP32 proteins. We map the enhanced proviral activity of swine ANP32A to a pair of amino acids, 106 and 156, in the leucine-rich repeat and central domains and show these mutations enhance binding to influenza virus trimeric polymerase. These findings help elucidate the molecular basis for the mixing vessel trait of swine and further our understanding of the evolution and ecology of viruses in this host. IMPORTANCE Avian influenza viruses can jump from wild birds and poultry into mammalian species such as humans or swine, but they only continue to transmit if they accumulate mammalian adapting mutations. Pigs appear uniquely susceptible to both avian and human strains of influenza and are often described as virus “mixing vessels.” In this study, we describe how a host factor responsible for regulating virus replication, ANP32A, is different between swine and humans. Swine ANP32A allows a greater range of influenza viruses, specifically those from birds, to replicate. It does this by binding the virus polymerase more tightly than the human version of the protein. This work helps to explain the unique properties of swine as mixing vessels.

scholarly journals Knockout of IRF7 Highlights its Modulator Function of Host Response Against Avian Influenza Virus and the Involvement of MAPK and TOR Signaling Pathways in Chicken

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 385
Author(s):  
Tae Hyun Kim ◽  
Colin Kern ◽  
Huaijun Zhou
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Signaling Pathways ◽  
Cell Lines ◽  
Host Response ◽  
Antiviral Response ◽  
Influenza Viruses ◽  
Interferon Response ◽  
Type I

Interferon regulatory factor 7 (IRF7) is known as the master transcription factor of the type I interferon response in mammalian species along with IRF3. Yet birds only have IRF7, while they are missing IRF3, with a smaller repertoire of immune-related genes, which leads to a distinctive immune response in chickens compared to in mammals. In order to understand the functional role of IRF7 in the regulation of the antiviral response against avian influenza virus in chickens, we generated IRF7-/- chicken embryonic fibroblast (DF-1) cell lines and respective controls (IRF7wt) by utilizing the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9) system. IRF7 knockout resulted in increased viral titers of low pathogenic avian influenza viruses. Further RNA-sequencing performed on H6N2-infected IRF7-/- and IRF7wt cell lines revealed that the deletion of IRF7 resulted in the significant down-regulation of antiviral effectors and the differential expression of genes in the MAPK (mitogen-activated protein kinase) and mTOR (mechanistic target of rapamycin) signaling pathways. Dynamic gene expression profiling of the host response between the wildtype and IRF7 knockout revealed potential signaling pathways involving AP1 (activator protein 1), NF-κB (nuclear factor kappa B) and inflammatory cytokines that may complement chicken IRF7. Our findings in this study provide novel insights that have not been reported previously, and lay a solid foundation for enhancing our understanding of the host antiviral response against the avian influenza virus in chickens.

scholarly journals A review of H5Nx avian influenza viruses

2019 ◽  
Vol 7 ◽  
pp. 251513551882162 ◽  
Author(s):  
Ivette A. Nuñez ◽  
Ted M. Ross
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Highly Pathogenic Avian Influenza ◽  
Influenza Viruses ◽  
Avian Influenza Viruses ◽  
Highly Pathogenic ◽  
Bird Populations ◽  
Pathogenic Avian Influenza ◽  
Pathogenic Avian Influenza Virus

Highly pathogenic avian influenza viruses (HPAIVs), originating from the A/goose/Guangdong/1/1996 H5 subtype, naturally circulate in wild-bird populations, particularly waterfowl, and often spill over to infect domestic poultry. Occasionally, humans are infected with HPAVI H5N1 resulting in high mortality, but no sustained human-to-human transmission. In this review, the replication cycle, pathogenicity, evolution, spread, and transmission of HPAIVs of H5Nx subtypes, along with the host immune responses to Highly Pathogenic Avian Influenza Virus (HPAIV) infection and potential vaccination, are discussed. In addition, the potential mechanisms for Highly Pathogenic Avian Influenza Virus (HPAIV) H5 Reassorted Viruses H5N1, H5N2, H5N6, H5N8 (H5Nx) viruses to transmit, infect, and adapt to the human host are reviewed.

Risk factors for highly pathogenic H7N1 avian influenza virus infection in poultry during the 1999–2000 epidemic in Italy

2009 ◽  
Vol 181 (2) ◽  
pp. 171-177 ◽  
Author(s):  
Luca Busani ◽  
Maria Grazia Valsecchi ◽  
Emanuela Rossi ◽  
Marica Toson ◽  
Nicola Ferrè ◽  
...  
Keyword(s):  
Risk Factors ◽  
Influenza Virus ◽  
Virus Infection ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Influenza Virus Infection ◽  
Highly Pathogenic
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