scholarly journals Comparison of albumin, total protein, globulin and enzyme activity Creatine Phospho Kinase (CPK) in the Gingival Crevicular Fluid of patients with chronic periodontitis before and after treatment

2018 ◽  
Vol 6 (5) ◽  
Author(s):  
Ali Banihashem Rad ◽  
Shade Saghafi ◽  
Eshagh Hashemi ◽  
Ershad Aghasizadeh ◽  
Fathollah Ebrahimi ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Total Protein ◽  
Alveolar Bone ◽  
Gingival Crevicular Fluid ◽  
Phase 1 ◽  
Immunological Response ◽  
Treatment Phase ◽  
Significant Difference

Introduction: Periodontal disease is an inflammatory disease of the supporting tissues of the teeth caused by certain microorganisms and leads to progressive destruction of the periodontal ligament, alveolar bone with pocket formation or the recession, or both. Periodontal disease is usually based on clinical parameters diagnosed and recorded. Other advanced diagnostic techniques of periodontal disease is evaluation of host response, which includes the study of specific or non-specific mediators such as albumin, globulin and creatine phosphokinase enzyme by biochemical methods or immunological response that as part of the individual periodontal infections are known. Methods: The study population consisted of 30 patients. After observing isolation by cotton rolls, 50 to 100 ml GCF via 30 paper point and wetting with a height of 5 mm of the area with more involvement in terms of standard of patients with moderate periodontitis referred to Periodontics ward of Dental School in Mashhad pre-treatment phase 1 (scaling and root planning) and 4 weeks after the treatment is provided. Then changes in albumin, total protein, globulin and CPK enzyme activity in the GCF using biochemical kits measured. Result: In our study, mean CPK in patients with chronic periodontitis before treatment was further after treatment but not significantly different, but the average amount of albumin, total protein and globulin chronic periodontitis patients before treatment lesser than after treatment but there was no significant difference either. Conclusion: In this study, we showed that albumin, total protein and globulin was significantly increased after treatment of periodontitis patients.

scholarly journals Salivary Levels of IL-6 and IL-17 Could Be an Indicator of Disease Severity in Patients with Calculus Associated Chronic Periodontitis

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Husniah Batool ◽  
Ahmed Nadeem ◽  
Muhammad Kashif ◽  
Faheem Shahzad ◽  
Romeeza Tahir ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Alveolar Bone ◽  
Gingival Crevicular Fluid ◽  
Severe Disease ◽  
Cross Sectional Study ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Cross Sectional ◽  
Significant Difference ◽  
Crevicular Fluid

Background/Purpose. Chronic periodontitis is an inflammatory disease of gums that causes loss of supporting structures of teeth, that is, gingiva, periodontal ligament, cementum, and alveolar bone. Levels of various cytokines in the serum, gingival tissues, and gingival crevicular fluid in patients with chronic periodontitis have been studied, but limited data are available on the level of cytokines in saliva. Therefore, a study was designed to determine levels of salivary IL-6 and IL-17 in patients with calculus associated chronic periodontitis. Materials and Methods. It was a comparative, cross-sectional study that is comprised of 41 healthy controls and 41 calculus associated chronic periodontitis patients (CP patients). According to the degree of attachment loss, CP patients were subcategorized as mild (CAL 1-2 mm), moderate (CAL 3-4 mm), and severe (CAL > 5 mm) forms of periodontitis. Salivary levels of IL-6 and IL-17 were determined using enzyme-linked immunosorbent assay (ELISA) technique. Data was analyzed using SPSS 20.0. Results. Between healthy controls and CP patients (moderate and severe disease), a statistically significant difference was observed in the concentrations of IL-6 and IL-17. In CP patients, the highest mean ± SD of salivary IL-6 and IL-17 was observed in severe CP, followed by moderate and mild CP. Regarding level of IL-6, a statistically significant difference was observed between mild and severe disease and between moderate and severe subcategories of CP patients. Similarly, statistically significant difference was observed in the level of IL-17 between mild and moderate, mild and severe disease, and moderate and severe disease. Conclusion. The levels of salivary IL-6 and IL-17 were increased significantly in calculus associated CP patients as compared to healthy controls and these levels increased with the progression of CP. Clinical Significance. Salivary levels of IL-6 and IL-17 may help in the subcategorization of CP.

scholarly journals A COMPARATIVE STUDY TO ASSES THE EFFICACY OF NANOBIO FUSION GEL AS AN ADJUNCT TO SCALING AND POLISHING IN CHRONIC PERIODONTITIS. - A CLINICAL STUDY

2020 ◽  
pp. 1-3
Author(s):  
Patil A. Veena ◽  
Ansari T. Sobia ◽  
Agarwal Priyanka ◽  
Ayesha Ayesha ◽  
Sultana Shahnaaz
Keyword(s):  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Antimicrobial Agents ◽  
Test Group ◽  
Controlled Clinical Trial ◽  
Pocket Depth ◽  
Antioxidant Power ◽  
Probing Pocket Depth ◽  
Significant Difference ◽  
And Control

Introduction: Various chemical agents such as nonsteroidal, anti-inflammatory drugs and antimicrobial agents has gained popularity in treatment of periodontal disease but simultaneously lead to condition such as drug resistance and drug allergy. Hence , the topical application of herbal agents such as propolis, aloevera, green tea extracts, Neem reduces the potency and effectiveness to prevent progression of periodontal disease. NanoBioFusion(NBF)gel contains the natural antioxidant power of propolis,vit C,vit E which allows the ultrafine antioxidant to surpase the moist intraoral environment to enter the cells and rejuvenate,revitalize,support,protect and optimize gum and soft oral tissue.Hence the present study is aimed to evaluate the clinical effect of locally delivered NBF gel as an adjunctive therapy to scaling and polishing in the treatment of Periodontitis. Materials and Methods: Chronic Periodontitis patients with 40 sites and probing pocket depth (PD) between 5 and 7 mm were selected in a randomized controlled clinical trial. SRP was performed in both control and test group followed by NBF gel application in 40 sites. The plaque index, gingival index and probing Pocket depth,were recorded at baseline, 6 weeks, and 3 months.The statistical analysis with paired t‑test was used to compare the test and control sites. Results: From baseline to a period of 3 months, a statistically significant difference was seen between both groups for Pocket probing depth and from baseline to 6 weeks the mean GI and PI score have a statistically significant result was obtained(P=0.01& 0.00). Conclusions: Locally delivered NBF gel exhibited a significant improvement compared with SRP alone in chronic periodontitis.

scholarly journals Folate-receptor 1 level in periodontal disease: a pilot study

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Duygu Alkan ◽  
Berrak Guven ◽  
Cigdem Coskun Turer ◽  
Umut Balli ◽  
Murat Can
Keyword(s):  
Periodontal Disease ◽  
Gingival Crevicular Fluid ◽  
Folate Receptor ◽  
Serum Folate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Serum Samples ◽  
Pocket Depth ◽  
Probing Pocket Depth ◽  
Significant Difference

Abstract Background The purpose of this study was to investigate gingival crevicular fluid (GCF) and serum folate-receptor 1 (FOLR1) levels in subjects with different periodontal status. Methods The study consists of three groups: Healthy group (n = 15), gingivitis group (n = 15) and chronic periodontitis group (n = 15). Clinical periodontal parameters including probing pocket depth (PPD), clinical attachment level (CAL), gingival index (GI) and bleeding on probing (BOP) were assessed. GCF and serum samples were collected from each patient and were analyzed FOLR1 levels by enzyme-linked immunosorbent assay. Results The values of FOLR1 in GCF were higher in gingivitis and periodontitis groups than among patient in control group (p < 0.016). Serum FOLR1 levels showed no significant difference between the groups. A significant correlation was observed between FOLR1 levels of GCF and BOP (p < 0.05). Conclusions Our preliminary data suggest that FOLR1 is not useful in monitoring the periodontal disease. Further studies are necessary to clarify the role, regulation and function of folate and it’s receptors in the pathogenesis of periodontal disease.

scholarly journals Possible interaction between visfatin, periodontal infection, and other systemic diseases: A brief review of literature

2017 ◽  
Vol 11 (03) ◽  
pp. 407-410 ◽  
Author(s):  
Mojtaba Bayani ◽  
Mohammad Pourali ◽  
Mohammad Keivan
Keyword(s):  
Periodontal Disease ◽  
Bacterial Infections ◽  
Alveolar Bone ◽  
Gingival Crevicular Fluid ◽  
Systemic Diseases ◽  
Hand Search ◽  
Periodontal Infection ◽  
Etiologic Agents ◽  
Visfatin Level ◽  
Crevicular Fluid

ABSTRACTGingivitis and periodontitis are common bacterial infections caused by a variety of microorganisms. Despite the microorganisms' roles as etiologic agents, inflammation-induced substances also have crucial parts in the loss of connective tissue and the supporting alveolar bone. Visfatin is a pleiotropic mediator, which acts as growth factor, cytokine, and pre-B-cell colony-enhancing factor. A positive correlation was detected between the serum/plasma levels of visfatin and inflammatory disorders such as diabetes mellitus and cardiovascular disease. In addition, the visfatin level was higher in saliva and the gingival crevicular fluid (GCF) of subjects with periodontal disease. This review defined current, predictable patterns of possible interaction of visfatin with periodontal infection and other systemic diseases, using PubMed and Medline databases searching for articles written in English. Peer-reviewed articles were targeted using the following keywords: “visfatin,” “periodontal disease,” “inflammatory mediator,” and “biomarker.” Available full-text articles were read, and related articles were also scrutinized, while a hand search was also performed. Search was confined to human studies, and articles written in English and published between 1985 and 2016 were selected. It was concluded that periodontal infection and other systemic diseases could be related to the levels of visfatin in GCF, saliva, and serum as a biomarker of these diseases.

scholarly journals Assessment of supracrestal tissue attachment variation in patients with chronic periodontitis before and after treatment: A clinical-radiographic study

2020 ◽  
Vol 77 (11) ◽  
pp. 1175-1183
Author(s):  
Shahabe Abullais ◽  
Gore Anoop ◽  
Nitin Dani ◽  
Saad Al-qahatani ◽  
Ashfaq Yaqoob ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Clinical Findings ◽  
Scaling And Root Planing ◽  
Radiographic Findings ◽  
Root Planing ◽  
Significant Difference ◽  
Before And After ◽  
Tissue Attachment ◽  
Open Flap Debridement

Background/Aim. Healthy periodontium comprises the dento-gingival junction. Periodontal disease starts to appear when the integrity of the junctional epithelium is disturbed. Assessment of the supracrestal tissue attachment (SCTA) is essential because there is a frequent need for restoration or prosthesis after periodontal surgical and non-surgical therapy. The aim of the present study was to evaluate the SCTA variations in a patients with chronic periodontitis before and after treatment. Methods. Thirty systemically healthy patients with periodontitis were enrolled in the study. Fifteen patients were subjected to scaling and root planing and 15 to open flap debridement. Radiographic and clinical findings of the SCTA were assessed before and after treatment at 3-month and 6-month intervals. Results. Comparison between clinical and radiographic findings of the SCTA showed a significant difference in patients with periodontitis (p < 0.05). This difference was not significant after treatment of patients with shallow pockets with scaling and root planing (p > 0.05), but showed a significant difference in patients with moderate pockets treated by open flap debridement (p < 0.05). Conclusion. Progression in periodontal disease causes a reduction in the SCTA dimension, which regains its original dimensions after periodontal therapy. It takes around 3 months for the shallow pockets to regain the supracrestal tissue attachment to the original dimension when treated by scaling and root planing, whereas moderate pockets regain it after 6 months when treated with open flap debridement.

scholarly journals Effect of Obesity and/or Ligature-induced Periodontitis on Aortic Wall Thickness in Wistar Rats.

2020 ◽  
Vol 33 (1) ◽  
pp. 50-55
Author(s):  
Andressa Moreira ◽  
Alessandra Nicolini ◽  
Eduardo Gaio ◽  
Fernanda Visioli ◽  
Cassiano Rösing ◽  
...  
Keyword(s):  
Body Weight ◽  
Periodontal Disease ◽  
Wall Thickness ◽  
Wistar Rats ◽  
Aortic Wall ◽  
Alveolar Bone ◽  
Cafeteria Diet ◽  
Control Group ◽  
Significant Difference ◽  
Aortic Wall Thickness

The purpose of this study was to evaluate aortic wall thickness after periodontal disease and/or obesity induction in a Wistar rat model.Sixty male Wistar rats were randomly divided into four groups: control (CT), periodontal disease (PD), obesity (OB), and obesity plus periodontal disease (OB+PD). Groups OB and OB+PD received cafeteria diet for 17 weeks. After they had acquired obesity (week 12), periodontal disease was induced by placing a silk ligature on the maxillary right second molar of groups PD and OB+PD. During the experimental period, body weight and Lee index were assessed. Mean alveolar bone loss (ABL) was evaluated, and aortas were prepared for histometric analysis of the aortic wall by ImageJ software. Body weight and Lee index increased in rats exposed to cafeteria diet. Mean ABL was higher in Groups PD and OB+PD than in control and OB (p<0.05). ABL was 18% higher in Group OB+PD than in Group PD, with statistically significant difference (p<0.001). Aortas were thicker in Groups OB and OB+PD than in control and PD groups, respectively (2.31mm ± 0.28 and 2.33 ± 0.29 vs. 2.18 ± 0.26 and 2.14 ± 0.27). Group OB differed significantly from the control group (p=0.036), and OB+PD and OB differed significantly from PD (p=0.004 and p= 0.001, respectively). Obesity alters aortic wall thickness in Wistar rats. However, the presence of periodontal disease did not affect the aortic wall thickness under the conditions of the present study.

Efficacy of subgingivally delivered atorvastatin and simvastatin as an adjunct to scaling and root planing

2015 ◽  
Vol 30 (4) ◽  
Author(s):  
Sai M. Surve ◽  
Anirudh B. Acharya ◽  
Srinath L. Thakur
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Local Delivery ◽  
Control Group ◽  
Clinical Parameters ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Significant Difference ◽  
Group 2 ◽  
Crevicular Fluid

AbstractThe current understanding of the pathogenesis of periodontal disease has resulted in adjunctive use of various pharmacologic agents in periodontal therapy. The objective of this investigation was to assess the efficacy of atorvastatin and simvastatin (because of their pleiotropic properties) as an adjunct to dental scaling and root planing (SRP) by local delivery, i.e. placing them subgingivally, in the treatment of chronic periodontitis.Local delivery systems for atorvastatin and simvastatin were prepared in sodium alginate suspension to be administered with calcium chloride solution. Patients diagnosed with chronic periodontitis were grouped as group 1, receiving SRP only (control), group 2, receiving SRP with subgingival delivery of 1.2% simvastatin, and group 3, receiving SRP with subgingival delivery of 1.2% atorvastatin. Clinical parameters and interleukin (IL) 1α levels in the gingival crevicular fluid (GCF) were assessed.All three groups showed significant reductions in clinical parameters and IL-1α levels in the GCF (p<0.05). However, the test groups did not show any statistically significant difference when compared with control.Subgingivally delivered atorvastatin and simvastatin as an adjunct to SRP is efficacious but did not demonstrate any added benefit as compared with SRP alone.

scholarly journals Evaluation of IL6, IL10 and VDR alleles distribution in an Italian large sample of subjects affected by chronic periodontal disease

2019 ◽  
Vol 33 ◽  
pp. 205873841984084
Author(s):  
Luca Scapoli ◽  
Francesco Carinci ◽  
Damiano Mucchi ◽  
Alessandro Nota ◽  
Silvia Caruso ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Chronic Periodontitis ◽  
Point Of View ◽  
Population Heterogeneity ◽  
Inclusion Criteria ◽  
Allele Distribution ◽  
Chronic Periodontal Disease ◽  
Italian Regions ◽  
Significant Difference ◽  
Clinical Point

In recent decades, the role played by the immune response to bacteria in the pathogenesis of chronic periodontal disease (PD) has long been studied. Although from the clinical point of view, adequate oral hygiene is essential to ensure a satisfactory response of the host to infections, modulation of the reaction of immune system could be influenced by genetic factors. The aim of the present study was to investigate the distribution of alleles of polymorphisms relevant for chronic periodontitis in a sample of adult subjects affected by chronic PD. The present study was conducted with sample collected in Italian private practice offices from January 2013 to December 2017. The sample included 744 adult patients diagnosed with chronic periodontitis. The inclusion criteria were as follows: age > 18 years, diagnosis of chronic PD. The diagnosis of chronic periodontitis was based on the criteria established by the American Academy of Periodontology. No significant difference in allele distribution among patients from different Italian regions was found. Results, supporting absence of population heterogeneity for the investigated polymorphisms in Italy, suggest similar effect in periodontitis etiology.

Sign in / Sign up

Export Citation Format

Share Document