Structure Analysis and Diversity of Bacterial Community and their Resistance Determinants in a Nickel-Contaminated Soil in Southwest Slovakia

2016 ◽  
Vol 24 (1) ◽  
pp. 17-24
Author(s):  
Matej Remenár ◽  
Edita Karelová ◽  
Jana Harichová ◽  
Anna Kamlárová ◽  
Kristína Krčová ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Bacterial Community ◽  
Contaminated Soil ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Bacterial Phyla ◽  
Resistance Determinants ◽  
Final Output ◽  
Heavy Metal Resistant ◽  
Bioremediation Processes

AbstractIn this study we aimed to analyse the structure and diversity of overall bacterial community and its resistance determinants from nickel-contaminated soil in Slovakia by both, cultivation-dependent and independent approaches. The phylogeny was reconstructed using partial sequences of 16S rRNA (16S rDNA) and heavy-metal resistance genes from separated isolates and bacterial clones. A total of 518 bacterial sequences obtained from both, isolates and clones, represented 266 species belonging to 8 bacterial phyla: Acidobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Firmicutes, Gemmatimonadetes, Proteobacteria (α-, β- and γ-classes), Verrucomicrobia, and one yet unclassified group. In addition, among isolates and clones, 49 different nccA-like genes were found in the final output. Majority of them were assigned to a system of transmembrane metal pumps. Our results demonstrate the fact that the nickel-contaminated soil is able to present very specific heavy-metal resistant bacterial community which can be used in different bioremediation processes.

Structure analysis of bacterial community and their heavy-metal resistance determinants in the heavy-metal-contaminated soil sample

Biologia ◽  
2012 ◽  
Vol 67 (6) ◽  
Author(s):  
Jana Harichová ◽  
Edita Karelová ◽  
Domenico Pangallo ◽  
Peter Ferianc
Keyword(s):  
Heavy Metal ◽  
Soil Sample ◽  
16S Rdna ◽  
Resistance Genes ◽  
Contaminated Soil ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Resistance Determinants ◽  
Cultivable Bacteria ◽  
Rdna Sequences

AbstractIn this study we performed the phylogenetic analysis of non-cultivable bacteria from anthropogenically disturbed soil using partial sequences of the 16S rRNA (16S rDNA) and the heavy-metal resistance genes. This soil sample contained high concentrations of nickel (2,109 mg/kg), cobalt (355 mg/kg) and zinc (177 mg/kg), smaller concentrations of iron (35.75 mg/kg) and copper (32.2 mg/kg), and also a trace amount of cadmium (<0.25 mg/kg). The 16S rDNA sequences from a total of 74 bacterial clones were distributed into four broad taxonomic groups, Acidobacteria, Actinobacteria, Bacteroidetes and Gemmatimonadetes, and some of them were unidentified. Comparing our clone sequences with those from the GenBank database, only 9 clones displayed high similarity to known bacteria belongig to actinomycetes; others were identified as uncultured ones. Among clones evidently Actinobacteria predominated. Sixteen clones from soil sample carried only the nccA-like heavy-metal-resistance genes and all sequences showed too low similarity to known proteins encoded by these genes. However, our results suggested that the heavy-metal-contaminated soil is able to present very important reservoir of the new and until now unknown partly bacteria, partly heavy-metal-resistance determinants and their products. Bacteria and nccA-like genes identified in this study could represent the objects of interest as bioremediation agents because they can be potentially used in different transformation and immobilization processes.

scholarly journals Antibiotic and Heavy Metal Resistant Bacteria Isolated from Aegean Sea Water and Sediment in Güllük Bay, Turkey : Quantifying the resistance of identified bacteria species with potential for environmental remediation applications

2020 ◽  
Vol 64 (4) ◽  
pp. 507-525 ◽  
Author(s):  
Gülşen Altuğ ◽  
Mine Çardak ◽  
Pelin Saliha Çiftçi Türetken ◽  
Samet Kalkan ◽  
Sevan Gürün
Keyword(s):  
Heavy Metal ◽  
Aegean Sea ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Heavy Metal Resistant ◽  
Seawater Samples

Heavy metal and antibiotic-resistant bacteria have potential for environmental bioremediation applications. Resistant bacteria were investigated in sediment and seawater samples taken from the Aegean Sea, Turkey, between 2011 and 2013. Bioindicator bacteria in seawater samples were tested using the membrane filtration technique. The spread plate technique and VITEK® 2 Compact 30 micro identification system were used for heterotrophic aerobic bacteria in the samples. The minimum inhibition concentration method was used for heavy metal-resistant bacteria. Antibiotic-resistant bacteria were tested using the disk diffusion method. All bacteria isolated from sediment samples showed 100% resistance to rifampicin, sulfonamide, tetracycline and ampicillin. 98% of isolates were resistant against nitrofurantoin and oxytetracycline. Higher antibiotic and heavy metal resistance was recorded in bacteria isolated from sediment than seawater samples. The highest levels of bacterial metal resistance were recorded against copper (58.3%), zinc (33.8%), lead (32.1%), chromium (31%) and iron (25.2%). The results show that antibiotic and heavy metal resistance in bacteria from sediment and seawater can be observed as responses to environmental influences including pollution in marine areas.

scholarly journals Antibiotic Profiling of Heavy Metal Resistant Bacterial Isolates from the Effluent of a Garment Industry in Lalitpur, Nepal

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 203-206 ◽  
Author(s):  
M. Sharma ◽  
H.P. Thapaliya
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Heavy Metal Resistance ◽  
Garment Industry ◽  
Metal Resistance ◽  
Antibiotics Resistance ◽  
Bacterial Isolates ◽  
Heavy Metal Resistant ◽  
Multiple Antibiotics

Heavy metal resistant bacterial isolates from the effluent in a garment industry site were examined to assess their resistance towards multiple antibiotics. Heavy metal resistance property has been found to enhance the antibiotic resistance ability of microorganisms. Isolation of the heavy metal resistant organisms was done in media containing salts of heavy metals. Organisms were identified belonging to the genera Bacillus, Corynebacterium, Lactobacillus, Aeromonas and Enterococcus. Bacterial isolates were tested for their sensitivity to seven common antibiotics (penicillin, tetracycline, erythromycin, chloramphenicol, gentamicin, vancomycin and cotrimoxazole) using Kirby-Bauer technique. Isolates were found to be resistant to multiple antibiotics but all the isolates were sensitive to gentamicin. The data of our study indicates that metal pollution of the environment is the cause of heavy metal resistance isolates and hence antibiotic resistance.Key words: Heavy metal, effluent, antibiotics, resistance, Bacteria, pollution.DOI: 10.3126/on.v7i1.2572Our Nature (2009) 7:203-206  

scholarly journals Transcriptome Response to Heavy Metals in Sinorhizobium meliloti CCNWSX0020 Reveals New Metal Resistance Determinants That Also Promote Bioremediation by Medicago lupulina in Metal-Contaminated Soil

2017 ◽  
Vol 83 (20) ◽  
Author(s):  
Mingmei Lu ◽  
Shuo Jiao ◽  
Enting Gao ◽  
Xiuyong Song ◽  
Zhefei Li ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Sinorhizobium Meliloti ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Content Type ◽  
Resistance Determinants ◽  
Medicago Lupulina ◽  
Legume Symbiosis ◽  
Cu And Zn

ABSTRACT The symbiosis of the highly metal-resistant Sinorhizobium meliloti CCNWSX0020 and Medicago lupulina has been considered an efficient tool for bioremediation of heavy metal-polluted soils. However, the metal resistance mechanisms of S. meliloti CCNWSX00200 have not been elucidated in detail. Here we employed a comparative transcriptome approach to analyze the defense mechanisms of S. meliloti CCNWSX00200 against Cu or Zn exposure. Six highly upregulated transcripts involved in Cu and Zn resistance were identified through deletion mutagenesis, including genes encoding a multicopper oxidase (CueO), an outer membrane protein (Omp), sulfite oxidoreductases (YedYZ), and three hypothetical proteins (a CusA-like protein, a FixH-like protein, and an unknown protein), and the corresponding mutant strains showed various degrees of sensitivity to multiple metals. The Cu-sensitive mutant (ΔcueO) and three mutants that were both Cu and Zn sensitive (ΔyedYZ, ΔcusA-like, and ΔfixH-like) were selected for further study of the effects of these metal resistance determinants on bioremediation. The results showed that inoculation with the ΔcueO mutant severely inhibited infection establishment and nodulation of M. lupulina under Cu stress, while inoculation with the ΔyedYZ and ΔfixH-like mutants decreased just the early infection frequency and nodulation under Cu and Zn stresses. In contrast, inoculation with the ΔcusA-like mutant almost led to loss of the symbiotic capacity of M. lupulina to even grow in uncontaminated soil. Moreover, the antioxidant enzyme activity and metal accumulation in roots of M. lupulina inoculated with all mutants were lower than those with the wild-type strain. These results suggest that heavy metal resistance determinants may promote bioremediation by directly or indirectly influencing formation of the rhizobium-legume symbiosis. IMPORTANCE Rhizobium-legume symbiosis has been promoted as an appropriate tool for bioremediation of heavy metal-contaminated soils. Considering the plant-growth-promoting traits and survival advantage of metal-resistant rhizobia in contaminated environments, more heavy metal-resistant rhizobia and genetically manipulated strains were investigated. In view of the genetic diversity of metal resistance determinants in rhizobia, their effects on phytoremediation by the rhizobium-legume symbiosis must be different and depend on their specific assigned functions. Our work provides a better understanding of the mechanism of heavy metal resistance determinants involved in the rhizobium-legume symbiosis, and in further studies, genetically modified rhizobia harboring effective heavy metal resistance determinants may be engineered for the practical application of rhizobium-legume symbiosis for bioremediation in metal-contaminated soils.

scholarly journals Composition and Niche-Specific Characteristics of Microbial Consortia Colonizing Marsberg Copper Mine in the Rhenish Massif

2021 ◽  
Author(s):  
Sania Arif ◽  
Heiko Nacke ◽  
Elias Schliekmann ◽  
Andreas Reimer ◽  
Gernot Arp ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Heavy Metal Resistance ◽  
Resistance Mechanisms ◽  
Metal Resistance ◽  
Microbial Consortia ◽  
Rhenish Massif ◽  
Copper Mine ◽  
Alum Shale ◽  
Heavy Metal Resistant ◽  
Detoxification Pathways

Abstract. The Kilianstollen Marsberg (Rhenish Massif, Germany) has been extensively mined for copper ores, dating from Early Medieval Period till 1945. The exposed organic-rich alum shale rocks influenced by the diverse mine drainages at an ambient temperature of 10 °C could naturally enrich biogeochemically distinct heavy metal resistant microbiota. This metagenomic study evaluates the microbially colonized subterranean rocks of the abandoned copper mine Kilianstollen to characterize the colonization patterns and biogeochemical pathways of individual microbial groups. Under the selective pressure of the heavy metal contaminated environment at illuminated sites, Chloroflexi (Ktedonobacteria) and Cyanobacteria (Oxyphotobacteria) build up whitish-greenish biofilms. In contrast, Proteobacteria, Firmicutes and Actinobacteria dominate rocks around the uncontaminated spring water streams. The metagenomic analysis revealed that the heavy metal resistant microbiome was evidently involved in redox cycling of transition metals (Cu, Zn, Co, Ni, Mn, Fe, Cd, Hg). No deposition of metals or minerals, though, was observed by transmission electron microscopy in Ktedonobacteria biofilms which may be indicative for the presence of different detoxification pathways. The underlying heavy metal resistance mechanisms, as revealed by analysis of metagenome-assembled genomes, were mainly attributed to transition metal efflux pumps, redox enzymes, volatilization of Hg0, methylated intermediates of As(III) and reactive oxygen species detoxification pathways.

Heavy metal resistance in Arthrobacter ramosus strain G2 isolated from mercuric salt-contaminated soil

2010 ◽  
Vol 177 (1-3) ◽  
pp. 481-486 ◽  
Author(s):  
Amit Bafana ◽  
Kannan Krishnamurthi ◽  
Mahendra Patil ◽  
Tapan Chakrabarti
Keyword(s):  
Heavy Metal ◽  
Contaminated Soil ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Mercuric Salt

The isolation of heavy-metal resistant culturable bacteria and resistance determinants from a heavy-metal-contaminated site

Biologia ◽  
2011 ◽  
Vol 66 (1) ◽  
Author(s):  
Edita Karelová ◽  
Jana Harichová ◽  
Tatjana Stojnev ◽  
Domenico Pangallo ◽  
Peter Ferianc
Keyword(s):  
Heavy Metal ◽  
Phylogenetic Analysis ◽  
16S Rrna ◽  
16S Rdna ◽  
Minimal Medium ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Rich Medium ◽  
Resistance Determinants ◽  
Taxonomic Groups

AbstractIn this study we performed a phylogenetic analysis of a culturable bacterial community isolated from heavymetal-contaminated soil from southwest Slovakia using 16S rRNA (16S rDNA) and heavy-metal resistance genes. The soil sample contained high concentrations of nickel (2,109 mg/kg), cobalt (355 mg/kg) and zinc (177 mg/kg), smaller concentrations of iron (35.75 mg/kg) and copper (32.2 mg/kg), and a trace amount of cadmium (<0.25 mg/kg). A total of 100 isolates were grown on rich (Nutrient agar No. 2) or minimal (soil-extract agar medium) medium. The isolates were identified by phylogenetic analysis using partial sequences of their 16S rRNA (16S rDNA) genes. Representatives of two broad taxonomic groups, Firmicutes and Proteobacteria, were found on rich medium, whereas four taxonomic groups, Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria, were represented on minimal medium. Forty-two isolates grown on rich medium were assigned to 20 bacterial species, while 58 bacteria grown on minimal medium belonged to 49 species. Twenty-three isolates carried czcA- and/or nccA-like heavy-metal-resistance determinants. The heavy-metalresistance genes of nine isolates were identified by phylogenetic analysis of their protein sequences.

scholarly journals The Heavy-Metal Resistance Determinant of Newly Isolated Bacterium from a Nickel-Contaminated Soil in Southwest Slovakia

2018 ◽  
Vol 67 (2) ◽  
pp. 191-201 ◽  
Author(s):  
MATEJ REMENÁR ◽  
ANNA KAMLÁROVÁ ◽  
JANA HARICHOVÁ ◽  
MARCEL ZÁMOCKÝ ◽  
PETER FERIANC
Keyword(s):  
Heavy Metal ◽  
Contaminated Soil ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Resistance Determinant

scholarly journals Heavy Metal Tolerance Trend in Extended-Spectrum β-Lactamase Encoding Strains Recovered from Food Samples

2021 ◽  
Vol 18 (9) ◽  
pp. 4718
Author(s):  
Kashaf Junaid ◽  
Hasan Ejaz ◽  
Iram Asim ◽  
Sonia Younas ◽  
Humaira Yasmeen ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Food Samples ◽  
Esbl Production ◽  
Extended Spectrum ◽  
Retail Food ◽  
Esbl Producers

This study evaluates bacteriological profiles in ready-to-eat (RTE) foods and assesses antibiotic resistance, extended-spectrum β-lactamase (ESBL) production by gram-negative bacteria, and heavy metal tolerance. In total, 436 retail food samples were collected and cultured. The isolates were screened for ESBL production and molecular detection of ESBL-encoding genes. Furthermore, all isolates were evaluated for heavy metal tolerance. From 352 culture-positive samples, 406 g-negative bacteria were identified. Raw food samples were more often contaminated than refined food (84.71% vs. 76.32%). The predominant isolates were Klebsiella pneumoniae (n = 76), Enterobacter cloacae (n = 58), and Escherichia coli (n = 56). Overall, the percentage of ESBL producers was higher in raw food samples, although higher occurrences of ESBL-producing E. coli (p = 0.01) and Pseudomonas aeruginosa (p = 0.02) were observed in processed food samples. However, the prevalence of ESBL-producing Citrobacter freundii in raw food samples was high (p = 0.03). Among the isolates, 55% were blaCTX-M, 26% were blaSHV, and 19% were blaTEM. Notably, heavy metal resistance was highly prevalent in ESBL producers. These findings demonstrate that retail food samples are exposed to contaminants including antibiotics and heavy metals, endangering consumers.

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