Heavy Metal Tolerance Trend in Extended-Spectrum β-Lactamase Encoding Strains Recovered from Food Samples

This study evaluates bacteriological profiles in ready-to-eat (RTE) foods and assesses antibiotic resistance, extended-spectrum β-lactamase (ESBL) production by gram-negative bacteria, and heavy metal tolerance. In total, 436 retail food samples were collected and cultured. The isolates were screened for ESBL production and molecular detection of ESBL-encoding genes. Furthermore, all isolates were evaluated for heavy metal tolerance. From 352 culture-positive samples, 406 g-negative bacteria were identified. Raw food samples were more often contaminated than refined food (84.71% vs. 76.32%). The predominant isolates were Klebsiella pneumoniae (n = 76), Enterobacter cloacae (n = 58), and Escherichia coli (n = 56). Overall, the percentage of ESBL producers was higher in raw food samples, although higher occurrences of ESBL-producing E. coli (p = 0.01) and Pseudomonas aeruginosa (p = 0.02) were observed in processed food samples. However, the prevalence of ESBL-producing Citrobacter freundii in raw food samples was high (p = 0.03). Among the isolates, 55% were blaCTX-M, 26% were blaSHV, and 19% were blaTEM. Notably, heavy metal resistance was highly prevalent in ESBL producers. These findings demonstrate that retail food samples are exposed to contaminants including antibiotics and heavy metals, endangering consumers.

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Full-Length Nucleotide Sequences ofmcr-1-Harboring Plasmids Isolated from Extended-Spectrum-β-Lactamase-Producing Escherichia coli Isolates of Different Origins

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00935-16 ◽

2016 ◽

Vol 60 (9) ◽

pp. 5589-5591 ◽

Cited By ~ 44

Author(s):

Katrin Zurfluh ◽

Jochen Klumpp ◽

Magdalena Nüesch-Inderbinen ◽

Roger Stephan

Keyword(s):

Escherichia Coli ◽

Heavy Metal ◽

Heavy Metal Resistance ◽

Multidrug Resistant ◽

Metal Resistance ◽

Content Type ◽

Esbl Gene ◽

Extended Spectrum ◽

Different Origins ◽

First Time

ABSTRACTHere, we present the full sequences of threemcr-1-carrying plasmids isolated from extended-spectrum-β-lactamase (ESBL)-producingEscherichia coli. The plasmids belong to three different replicon types and are 34,640 bp, 209,401 bp, and 247,885 bp in size. We describe for the first time a composite transposon containingmcr-1localized on a multidrug-resistant (MDR) IncHI2 plasmid harboring additional determinants of resistance to six different classes of antibiotics, including the ESBL geneblaCTX-M-1, and heavy metal resistance.

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Extended-spectrum β-lactamase characterisation and heavy metal resistance of Enterobacteriaceae strains isolated from hospital environmental surfaces

Journal of Hospital Infection ◽

10.1016/j.jhin.2010.01.001 ◽

2010 ◽

Vol 75 (1) ◽

pp. 78-79 ◽

Cited By ~ 11

Author(s):

A. Touati ◽

K. Zenati ◽

L. Brasme ◽

S. Benallaoua ◽

C. de Champs

Keyword(s):

Heavy Metal ◽

Heavy Metal Resistance ◽

Metal Resistance ◽

Extended Spectrum ◽

Environmental Surfaces

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Evaluation of bacterial strains isolated from Late Quaternary alluvial sediments spanning ~ 28 m in depth for heavy metal tolerance and Cr(VI) removal ability

International Microbiology ◽

10.1007/s10123-021-00174-0 ◽

2021 ◽

Author(s):

Abhi P. Shah ◽

G. Archana

Keyword(s):

Heavy Metal ◽

Metal Tolerance ◽

Late Quaternary ◽

Heavy Metal Tolerance ◽

Bacterial Strains ◽

Alluvial Sediments

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Effect of Lead and Copper on Photosynthetic Apparatus in Citrus (Citrus aurantium L.) Plants. The Role of Antioxidants in Oxidative Damage as a Response to Heavy Metal Stress

Plants ◽

10.3390/plants10010155 ◽

2021 ◽

Vol 10 (1) ◽

pp. 155

Author(s):

Anastasia Giannakoula ◽

Ioannis Therios ◽

Christos Chatzissavvidis

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Heavy Metal ◽

Metal Tolerance ◽

Heavy Metal Tolerance ◽

Membrane Integrity ◽

Photosynthetic Apparatus ◽

Heavy Metal Stress ◽

Photosynthetic Parameters ◽

Citrus Aurantium

Photosynthetic changes and antioxidant activity to oxidative stress were evaluated in sour orange (Citrus aurantium L.) leaves subjected to lead (Pb), copper (Cu) and also Pb + Cu toxicity treatments, in order to elucidate the mechanisms involved in heavy metal tolerance. The simultaneous effect of Pb− and Cu on growth, concentration of malondialdehyde (MDA), hydrogen peroxide (H2O2), chlorophylls, flavonoids, carotenoids, phenolics, chlorophyll fluorescence and photosynthetic parameters were examined in leaves of Citrus aurantium L. plants. Exogenous application of Pb and Cu resulted in an increase in leaf H2O2 and lipid peroxidation (MDA). Toxicity symptoms of both Pb and Cu treated plants were stunted growth and decreased pigments concentration. Furthermore, photosynthetic activity of treated plants exhibited a significant decline. The inhibition of growth in Pb and Cu-treated plants was accompanied by oxidative stress, as indicated by the enhanced lipid peroxidation and the high H2O2 concentration. Furthermore, antioxidants in citrus plants after exposure to high Pb and Cu concentrations were significantly increased compared to control and low Pb and Cu treatments. In conclusion, this study indicates that Pb and Cu promote lipid peroxidation, disrupt membrane integrity, reduces growth and photosynthesis and inhibit mineral nutrition. Considering the potential for adverse human health effects associated with high concentrations of Pb and Cu contained in edible parts of citrus plants the study signals that it is important to conduct further research into the accessibility and uptake of the tested heavy metals in the soil and whether they pose risks to humans.

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Genome-Driven Discovery of Enzymes with Industrial Implications from the Genus Aneurinibacillus

Microorganisms ◽

10.3390/microorganisms9030499 ◽

2021 ◽

Vol 9 (3) ◽

pp. 499

Author(s):

Majid Rasool Kamli ◽

Nada A. Y. Alzahrani ◽

Nahid H. Hajrah ◽

Jamal S. M. Sabir ◽

Adeel Malik

Keyword(s):

Heavy Metal ◽

Enzyme Activities ◽

Heavy Metal Resistance ◽

Industrial Applications ◽

Metal Resistance ◽

The Family ◽

Antiviral Activities ◽

Genome Level ◽

Genome Analyses ◽

Unique Species

Bacteria belonging to the genus Aneurinibacillus within the family Paenibacillaceae are Gram-positive, endospore-forming, and rod-shaped bacteria inhabiting diverse environments. Currently, there are eight validly described species of Aneurinibacillus; however, several unclassified species have also been reported. Aneurinibacillus spp. have shown the potential for producing secondary metabolites (SMs) and demonstrated diverse types of enzyme activities. These features make them promising candidates with industrial implications. At present, genomes of 9 unique species from the genus Aneurinibacillus are available, which can be utilized to decipher invaluable information on their biosynthetic potential as well as enzyme activities. In this work, we performed the comparative genome analyses of nine Aneurinibacillus species representing the first such comprehensive study of this genus at the genome level. We focused on discovering the biosynthetic, biodegradation, and heavy metal resistance potential of this under-investigated genus. The results indicate that the genomes of Aneurinibacillus contain SM-producing regions with diverse bioactivities, including antimicrobial and antiviral activities. Several carbohydrate-active enzymes (CAZymes) and genes involved in heavy metal resistance were also identified. Additionally, a broad range of enzyme classes were also identified in the Aneurinibacillus pan-genomes, making this group of bacteria potential candidates for future investigations with industrial applications.

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Molecular mechanistic model of plant heavy metal tolerance

BioMetals ◽

10.1007/s10534-012-9541-y ◽

2012 ◽

Vol 25 (3) ◽

pp. 489-505 ◽

Cited By ~ 61

Author(s):

Ganesh Thapa ◽

Ayan Sadhukhan ◽

Sanjib Kumar Panda ◽

Lingaraj Sahoo

Keyword(s):

Heavy Metal ◽

Metal Tolerance ◽

Mechanistic Model ◽

Heavy Metal Tolerance

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Retromobilization of heavy metal resistance genes in unpolluted and heavy metal polluted soil

FEMS Microbiology Ecology ◽

10.1111/j.1574-6941.1995.tb00176.x ◽

1995 ◽

Vol 18 (3) ◽

pp. 191-203 ◽

Cited By ~ 22

Author(s):

Eva M. Top ◽

Helene Rore ◽

Jean-Marc Collard ◽

Veerle Gellens ◽

Galina Slobodkina ◽

...

Keyword(s):

Heavy Metal ◽

Resistance Genes ◽

Heavy Metal Resistance ◽

Metal Resistance ◽

Polluted Soil

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Identification and characterization of eight metallothionein genes involved in heavy metal tolerance from the ectomycorrhizal fungus Laccaria bicolor

Environmental Science and Pollution Research ◽

10.1007/s11356-021-16776-0 ◽

2021 ◽

Author(s):

Binhao Liu ◽

Pengcheng Dong ◽

Xinzhe Zhang ◽

Zhihang Feng ◽

Zhugui Wen ◽

...

Keyword(s):

Heavy Metal ◽

Metal Tolerance ◽

Heavy Metal Tolerance ◽

Ectomycorrhizal Fungus ◽

Laccaria Bicolor ◽

Identification And Characterization

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Screening of heavy metal and antibiotic resistance among Proteus vulgaris isolates from hospital wastewater of Northern India

Anti-Infective Agents ◽

10.2174/2211352519666210308101621 ◽

2021 ◽

Vol 19 ◽

Author(s):

Manzar Alam ◽

Mohd Imran ◽

Syed Sayeed Ahmad

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Multiple Drug Resistance ◽

Heavy Metal Resistance ◽

Metal Resistance ◽

Antibiotics Resistance ◽

Proteus Vulgaris ◽

Resistance To Antibiotics ◽

Transfer Frequency ◽

Resistance Patterns

Background: Microbial resistance to antibiotics and heavy metals is a rising problem in the world today. All the Proteus vulgaris isolates showed their MIC in between 50-1600 µg/ml. Of 70% and 46% of the isolates showed their MIC at 800-1200 µg/ml against Zn2+ and Cu2+ while 80% of the isolates showed their MIC at 100-200 µg/ml against Ni2+, respectively. All Proteus vulgaris isolates also exhibited multiple resistance patterns (2-7 heavy metals) in different combination of metals. The Multi metal resistance Index (MHMR) ranges were found (0.04-0.5). Methods: A high level of antibiotics resistance was observed against Methicillin (100%) and least to Oflaxicin (6%), Gentamycine and Neomycin (10%). All Proteus vulgaris isolates also showed multiple drug resistance patterns (2-12 antibiotics) in different combination of antibiotics. The MAR index ranges were found (0.02-0.7). Of 98%, 84% and 80% of the total isolates showed urease, gelatinase and amylase activity. Results: The Proteus vulgaris isolates contained plasmid of size ranging from 42.5 to 57.0kb and molecular weight of plasmids ranged from 27.2 to 37.0 MD. Incidences of resistance transfer, 7 pairs of isolates were assessed for the transfer of the antibiotic/ heavy metal resistance markers. The higher (4.4x10-1 and 3.4x10-1) transfer frequency was observed among antibiotic and heavy metal while lower transfer frequency were (5.0x10-2 and 1.0x10-2) showed against antibiotic and heavy metal in both the medium from the entire site tested, respectively. Conclusion: Indicating the high threat of environmental pollution and appearance of heavy metal resistance which may support the enlargement of resistance to antibiotics among the pathogens.

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