Molecular phylogeny and ultrastructure of Myxobolus cf. cuneus, a parasite of patinga hybrid and Henneguya pseudoplatystoma, a parasite of pintado hybrid

2015 ◽  
Vol 60 (3) ◽  
Author(s):  
Tiago Milanin ◽  
Antônio Augusto Mendes Maia ◽  
Márcia Ramos Monteiro Silva ◽  
Mateus Maldonado Carriero ◽  
Edson Aparecido Adriano
Keyword(s):  
Phylogenetic Analysis ◽  
Maximum Likelihood ◽  
Molecular Phylogeny ◽  
Developmental Stages ◽  
Fish Farms ◽  
Piaractus Mesopotamicus ◽  
Asynchronous Development ◽  
Rdna Sequencing ◽  
Hybrid Fish ◽  
Host Parasite

AbstractThrough morphological, histopathological and ultrastructural analysis of Myxobolus cuneus Adriano, Arana et Cordeiro, 2006 and Henneguya pseudoplatystoma Naldoni, Arana, Maia, Ceccarelli, Tavares, Borges, Pozo et Adriano, 2009 were identified infecting pacu respectively (Piaractus mesopotamicus) and hybrid pintado (Pseudoplatystoma corruscans x Pseudoplatystoma reticulatum) taken from Brazilian fish farms. The present study describes 18S rDNA sequencing of Myxobolus cf. cuneus infecting the spleen of farmed patinga, a hybrid fish resulting from the crossing of P. mesopotamicus x Piaractus brachypomus, and H. pseudoplatystoma found in farmed hybrid pintado from the state of Sao Paulo, Brazil. The study also provides new details of the host-parasite interface of M. cf. cuneus, which reveal that the plasmodial wall is composed of a single membrane connected to the plasmodium ectoplasm by numerous pinocytic canals. The plasmodia also displayed asynchronous development but had disporic pansporoblasts at different developmental stages; immature and mature spores were found at different depth levels of the plasmodium. Maximum likelihood phylogenetic analysis showed that M. cf. cuneus appeared as a sister species of Henneguya pellucida Adriano, Arana et Cordeiro, 2005 in a sub-clade composed mainly of myxosporean parasites of characiforms, and that H. pseudoplatystoma clustered in a sub-clade composed of Henneguya/Myxobolus spp. parasites of siluriform fish.

Molecular phylogeny of the butterfly tribe Baorini Doherty, 1886 (Hesperiidae, Hesperiinae) in China

Zootaxa ◽  
2019 ◽  
Vol 4565 (1) ◽  
pp. 108 ◽  
Author(s):  
WEIBIN JIANG ◽  
HAIYAN HE ◽  
YUANYUAN LI ◽  
YING WANG ◽  
CHEN GE ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Maximum Likelihood ◽  
Species Diversity ◽  
Molecular Phylogeny ◽  
Phylogenetic Relationships ◽  
Mitochondrial Genes ◽  
Nuclear Genes ◽  
Large Group ◽  
The Status

The butterfly tribe Baorini Doherty, 1886 is a large group of skippers. In this study, a total of 8 genera and 41 species of putative members of this tribe, which represent most of the generic diversity and nearly all the species diversity of the group in China, were sequenced for two mitochondrial genes and three nuclear genes (2084 bp). Phylogenetic relationships and subdivision of this tribe were investigated and the status of the genera are discussed. Partitioned maximum likelihood analyses were performed based on the combined dataset. Our results suggest that the data are split into two well-supported clades in the phylogeny tree. This analysis also represents the most complete phylogenetic analysis of the tribe Baorini in China to date, and includes several genera and species that have been previously excluded from published phylogenies of this group. 

Three new species of Entoloma subgenus Nolanea from India based on morphology and molecular phylogeny

Phytotaxa ◽  
2016 ◽  
Vol 286 (4) ◽  
pp. 232 ◽  
Author(s):  
K. N.ANIL RAJ ◽  
PATINJAREVEETTIL MANIMOHAN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Maximum Likelihood ◽  
Molecular Phylogeny ◽  
Related Species ◽  
Internal Transcribed Spacer ◽  
Internal Transcribed Spacer Region ◽  
Three New Species ◽  
Kerala State

Three new species of Entoloma belonging to the subgenus Nolanea, E. brunneoumbonatum, E. luteodiscum and E. brunneoapplanatum, are described from Kerala State, India based on morphology and molecular phylogeny. Comprehensive descriptions, photographs, and comparisons with phenetically similar and phylogenetically related species are discussed. The nuclear ribosomal internal transcribed spacer region (ITS) of the three species was sequenced and analyzed. The ITS-based phylogenetic analysis, performed using Maximum Likelihood (ML) method, supported both the novelty of these species and their infrageneric placement within the subgenus Nolanea. A key to the ten species of Entoloma subgenus Nolanea known from India is also provided.

scholarly journals Molecular Phylogeny of Jacanas and its Implications for Morphologic and Biogeographic Evolution

The Auk ◽  
2000 ◽  
Vol 117 (1) ◽  
pp. 22-32 ◽  
Author(s):  
Linda A. Whittingham ◽  
Frederick H. Sheldon ◽  
Stephen T. Emlen
Keyword(s):  
Phylogenetic Analysis ◽  
Maximum Likelihood ◽  
Molecular Phylogeny ◽  
Cytochrome B ◽  
New World ◽  
Mitochondrial Cytochrome ◽  
Biogeographic Pattern ◽  
Mitochondrial Cytochrome B

Abstract We compared sequences of mitochondrial cytochrome-b and ND5 genes in a phylogenetic analysis of seven species of jacanas, representing all six genera and including the Greater Painted-snipe (Rostratula benghalensis) as an outgroup. When analyzed separately by parsimony and maximum-likelihood bootstrapping, the two genes produced consistent trees, although the ND5 tree was better resolved than the cytochrome-b tree. When combined, the data from the two genes produced a fully resolved tree that was identical to the ND5 tree. This tree had the following form: ((((Irediparra, Microparra), Metopidius), Actophilornis), ((Jacana jacana, J. spinosa), Hydrophasianus)), Rostratula. The phylogeny consists of two major clades that were known to traditional and phylogenetic taxonomists. It also contains sister taxa that are geographically disjunct: the New World Jacana and Asian Hydrophasianus, and the African Microparra and Australian Irediparra. We postulate that this biogeographic pattern results from the extinction of intervening African and Asian taxa, respectively.

scholarly journals A new species of the genus Euxaldar Fennah, 1978 (Hemiptera, Fulgoromorpha, Issidae) from China and revision on the molecular phylogeny of the family

ZooKeys ◽  
2021 ◽  
Vol 1021 ◽  
pp. 19-35
Author(s):  
Liang-Jing Yang ◽  
Zhi-Min Chang ◽  
Lin Yang ◽  
Xiang-Sheng Chen
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Bayesian Inference ◽  
Maximum Likelihood ◽  
Molecular Phylogeny ◽  
Molecular Phylogenetic Analysis ◽  
The Family ◽  
Molecular Phylogenetic ◽  
First Time ◽  
A New Species

A new species Euxaldar daweishanensis Yang, Chang & Chen, sp. nov. is described and illustrated from southwestern China. The female genitalia of the genus Euxaldar is described and presented for the first time. A checklist and key to the known species of the genus are provided. A revised molecular phylogenetic analysis of the family Issidae based on combined partial sequences of 18S, 28S, COI, and Cytb is provided using both Maximum likelihood and Bayesian inference analyses.

scholarly journals A new genus Vittaliana belonging to the tribe Opsiini (Hemiptera: Cicadellidae) from India and its molecular phylogeny

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9515
Author(s):  
Sunil ◽  
Naresh M. Meshram ◽  
Tahseen Raza Hashmi ◽  
Pathour R. Shashank
Keyword(s):  
Phylogenetic Analysis ◽  
Maximum Likelihood ◽  
Molecular Phylogeny ◽  
Anterior Margin ◽  
New Genus ◽  
The Face ◽  
Branch Support ◽  
Hishimonus Phycitis ◽  
Combined Data

The new leafhopper genus Vittalianareticulata gen. nov., sp. nov., is described from India, and placed in the tribe Opsiini based on ocelli close to eyes, without carina on anterior margin of the face and bifurcate aedeagus with two gonopores. Phylogenetic analysis with maximum likelihood (ML) using IQtree v1.4.1 of combined data (Histone H3 and 28S rDNA) reveals that the new genus Vittaliana belongs to a clade consisting of Opsius versicolor (Distant, 1908), Opsiini gen. sp., Libengaia sp., Hishimonus phycitis (Distant, 1908) and Yinfomibus menglaensis Du, Liang & Dai (2019) with good branch support, and that the tribe Opsiini is paraphyletic. This resolves the placement of a new genus in the tribe Opsiini under Deltocephalinae.

scholarly journals Extensive Phylogenetic Analysis of Piscine Orthoreovirus Genomic Sequences Shows the Robustness of Subgenotype Classification

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 41
Author(s):  
Marcos Godoy ◽  
Daniel A. Medina ◽  
Rudy Suarez ◽  
Sandro Valenzuela ◽  
Jaime Romero ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genetic Variation ◽  
Sequence Analysis ◽  
Molecular Phylogeny ◽  
Phylogenetic Trees ◽  
Genomic Segment ◽  
Double Stranded Rna ◽  
The Family ◽  
Original Classification

Piscine orthoreovirus (PRV) belongs to the family Reoviridae and has been described mainly in association with salmonid infections. The genome of PRV consists of about 23,600 bp, with 10 segments of double-stranded RNA, classified as small (S1 to S4), medium (M1, M2 and M3) and large (L1, L2 and L3); these range approximately from 1000 bp (segment S4) to 4000 bp (segment L1). How the genetic variation among PRV strains affects the virulence for salmonids is still poorly understood. The aim of this study was to describe the molecular phylogeny of PRV based on an extensive sequence analysis of the S1 and M2 segments of PRV available in the GenBank database to date (May 2020). The analysis was extended to include new PRV sequences for S1 and M2 segments. In addition, subgenotype classifications were assigned to previously published unclassified sequences. It was concluded that the phylogenetic trees are consistent with the original classification using the PRV genomic segment S1, which differentiates PRV into two major genotypes, I and II, and each of these into two subgenotypes, designated as Ia and Ib, and IIa and IIb, respectively. Moreover, some clusters of country- and host-specific PRV subgenotypes were observed in the subset of sequences used. This work strengthens the subgenotype classification of PRV based on the S1 segment and can be used to enhance research on the virulence of PRV.

Developmental stages, incubation temperature, and in vivo traceability of primordial germ cell in an important aquaculture species Piaractus mesopotamicus

Aquaculture ◽  
2021 ◽  
Vol 535 ◽  
pp. 736381
Author(s):  
Geovanna Carla Zacheo Coelho ◽  
Dilberto Ribeiro Arashiro ◽  
Tamiris Disselli ◽  
Matheus Pereira-Santos ◽  
Tatiana María Mira-López ◽  
...  
Keyword(s):  
Germ Cell ◽  
Developmental Stages ◽  
Incubation Temperature ◽  
Primordial Germ Cell ◽  
Piaractus Mesopotamicus

Ultrastructural and molecular characterization of Bacillidium vesiculoformis n. sp. (Microspora: Mrazekiidae) in the freshwater oligochaete Nais simplex (Oligochaeta: Naididae)

Parasitology ◽  
2004 ◽  
Vol 130 (1) ◽  
pp. 31-40 ◽  
Author(s):  
D. J. MORRIS ◽  
R. S. TERRY ◽  
K. B. FERGUSON ◽  
J. E. SMITH ◽  
A. ADAMS
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Infected Cell ◽  
Host Cell ◽  
Developmental Stages ◽  
Parasite Development ◽  
Adhesive Quality ◽  
Transmission Studies ◽  
A New Species

The development of a new species, Bacillidium vesiculoformis n. sp. (Microspora, Mrazekiidae), is described from the freshwater oligochaete Nais simplex (Oligochaeta, Naididae). Initial stages of parasite development consist of a monokaryotic merogony within a haemocyte of the intestinal blood sinus. The resulting hypertrophied haemocyte is attached to the chloragocytes of the sinus by fine cytoplasmic extensions with the sinus around the cell becoming greatly enlarged. The meronts within the haemocyte form diplokaryotic sporonts that undergo sporogenesis directly within the cytoplasm of the host cell. The infected cell becomes packed with spores and developmental stages, causing it dramatically to increase in size, eventually rupturing the oligochaete and cell. Sporogony appears to be disporoblastic. Released spores were observed to have an adhesive quality. Transmission studies conducted with mature spores failed to transmit the parasite horizontally although vertical transmission was observed. Phylogenetic analysis of the parasite demonstrated that B. vesiculoformis clustered with microsporidian parasites of bryozoa and two other microsporidians, Janacekia debaiseuxi and an unidentified Bacillidium sp.

scholarly journals Parasitological assessment in the hybrid surubim (Pseudoplatystoma reticulatum x P. corruscans), with uncommon occurrence of Monogenea parasites

2016 ◽  
Vol 25 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Gabriela Tomas Jerônimo ◽  
Santiago Benites de Pádua ◽  
Arlene Sobrinho Ventura ◽  
Eduardo Luis Tavares Gonçalves ◽  
Márcia Mayumi Ishikawa ◽  
...  
Keyword(s):  
Management Practices ◽  
Cold Season ◽  
Parasite Fauna ◽  
Ichthyophthirius Multifiliis ◽  
Fish Farms ◽  
Mato Grosso ◽  
Central Brazil ◽  
Ecological Concern ◽  
Host Parasite ◽  
Mato Grosso Do Sul

Abstract This study evaluated the parasite fauna of farmed hybrid surubim (Pseudoplatystoma reticulatum x P. corruscans) and the host-parasite-environment relationship in two fish farms located in Mato Grosso do Sul, Central Brazil, South America. A total of 120 hybrids from two different farms, 60 in each season (30 in the hot and 30 in cold season) were examined during a year. Water quality was weekly measured to evaluate the interaction among environmental conditions and parasitism. Histopathology was used to observe the effects of the parasites and environment on the fish gills. The ciliate protozoan Ichthyophthirius multifiliis and the monogeneans (Ameloblastella sp., Amphocleithrium paraguayensis, Vancleaveus ciccinus, V. fungulus and V. janacauensis) were the most prevalent parasites detected in both seasons in both farms, with prevalence above 80%. It was stated that parasites did not cause important damage in the health status of the hybrid surubim. These results might be related to general good management practices and environmental quality implemented by the fish farmers. The presence of uncommon monogenean parasites to this hybrid compared to their parents causing an environmental and ecological concern is here discussed.

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