First report of Schulzia travassosi (Nematoda, Trichostrongylina, Molineoidea) for amphibians of the Chaco region in Argentina and proposal of Oswaldocruzia melanostictusi nov. comb.

2015 ◽  
Vol 60 (4) ◽  
Author(s):  
Cynthya E. González ◽  
Monika I. Hamann
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
First Record ◽  
Nematode Parasite ◽  
First Report ◽  
Chaco Region ◽  
Males And Females ◽  
Almost All ◽  
Chaco Province ◽  
Scanning Electron

AbstractThis is the first record of the nematode parasite Schulzia travassosi (Trichostrongylina, Molineidae) in two Argentinean amphibians, Leptodactylus bufonius and Rhinella major, collected in two localities of the Chaco region, Las Lomitas (Formosa Province) and Taco Pozo (Chaco Province). The species was observed using light and scanning electron microscopy (SEM) and compared with previous studies of Paraguayan specimens. Regarding metric characters, the measurements found in Argentinean specimens generally did not differ from those of Paraguayan specimens; some differences were observed in the total length of males and females (some longer and some shorter than Paraguayan specimens), length of the oesophagus (in almost all specimens shorter than in Paraguayan ones) and position of the vulva (nearer to posterior end in Argentinean specimens). The branches and sphincter of ovejector are slightly longer than in Paraguayan female specimens. In males, ray 4 is almost the same length as rays 2, 3 and 5, 6. Cephalic end, cephalic vesicle, synlophe and vulvar depression were viewed under SEM. Schulzia melanostictusi Chopra, Shing and Kumar, 1986 is reassigned as Oswaldocruzia melanostictusi (Chopra, Shing and Kumar, 1986) nov. comb.

scholarly journals Synthesis and Evaluation of AlgNa-g-poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone

2021 ◽  
Vol 22 (11) ◽  
pp. 5730
Author(s):  
Jomarien García-Couce ◽  
Marioly Vernhes ◽  
Nancy Bada ◽  
Lissette Agüero ◽  
Oscar Valdés ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Controlled Release ◽  
Biomedical Applications ◽  
Release Kinetics ◽  
Cell Types ◽  
Tissue Engineering Scaffolds ◽  
Almost All ◽  
Scanning Electron

Hydrogels obtained from combining different polymers are an interesting strategy for developing controlled release system platforms and tissue engineering scaffolds. In this study, the applicability of sodium alginate-g-(QCL-co-HEMA) hydrogels for these biomedical applications was evaluated. Hydrogels were synthesized by free-radical polymerization using a different concentration of the components. The hydrogels were characterized by Fourier transform-infrared spectroscopy, scanning electron microscopy, and a swelling degree. Betamethasone release as well as the in vitro cytocompatibility with chondrocytes and fibroblast cells were also evaluated. Scanning electron microscopy confirmed the porous surface morphology of the hydrogels in all cases. The swelling percent was determined at a different pH and was observed to be pH-sensitive. The controlled release behavior of betamethasone from the matrices was investigated in PBS media (pH = 7.4) and the drug was released in a controlled manner for up to 8 h. Human chondrocytes and fibroblasts were cultured on the hydrogels. The MTS assay showed that almost all hydrogels are cytocompatibles and an increase of proliferation in both cell types after one week of incubation was observed by the Live/Dead® assay. These results demonstrate that these hydrogels are attractive materials for pharmaceutical and biomedical applications due to their characteristics, their release kinetics, and biocompatibility.

scholarly journals FIRST REPORT ON SURFACE ASPECTS OF Mansonella ozzardi (SPIRURIDA: ONCHOCERCIDAE) MICROFILARIAE BY SCANNING ELECTRON MICROSCOPY: PRELIMINARY RESULTS

2018 ◽  
Vol 47 (3) ◽  
pp. 195
Author(s):  
Yara Leite Adami ◽  
Aleksandra Menezes de Oliveira ◽  
Reinalda Marize Lanfredi in memorian ◽  
Marilza Maia-Herzog
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
First Report ◽  
Preliminary Results ◽  
Scanning Electron

Antennal Sensilla of Chrysis shanghalensis (Hymenoptera: Chrysididae), a Larval Ectoparasite of Monema flavescens (Lepidoptera: Limacodidae)

2021 ◽  
Vol 56 (1) ◽  
pp. 1-11
Author(s):  
Shu Z. Yang ◽  
Mei H. Yang ◽  
Yun Xu ◽  
Jin T. Zhang
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Natural Enemy ◽  
Tree Species ◽  
External Morphology ◽  
Antennal Sensilla ◽  
Sensilla Basiconica ◽  
Sensilla Trichodea ◽  
Males And Females ◽  
Scanning Electron

Abstract Chrysis shanghalensis Smith (Hymenoptera: Chrysididae) is an ectoparasitoid and important natural enemy of Monema flavescens Walker (Lepidoptera: Limacodidae), a serious defoliator of a number of tree species. The external morphology of the antennal sensilla of this parasitoid was examined by scanning electron microscopy, and types and distribution of sensilla were recorded. The antennae of C. shanghalensis were geniculate in shape and composed of a scape with radicula, a pedicel, and a flagellum divided into 11 flagellomeres in males and females. Cuticular pore and 14 types of sensilla were identified on the antennae of both sexes. These included aporous Böhm's bristles, sensilla trichodea 1, 2, and 3 (nonporous), sensilla trichodea 4 (multiporous), two types of sensilla chaetica (nonporous), four morphological types of sensilla basiconica (multiporous), two morphological types of sensilla coeloconica (multiporous), and one type of sensillum campaniforme (nonporous).

Scanning electron microscopy of lattice organs in cyprids of the Rhizocephala Akentrogonida (Crustacea: Cirripedia)

1994 ◽  
Vol 72 (6) ◽  
pp. 1018-1026 ◽  
Author(s):  
Peter Gram Jensen ◽  
Jens T. Høeg ◽  
Susan Bower ◽  
Alexey V. Rybakov
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Sister Group ◽  
Poor Quality ◽  
Sister Group Relationship ◽  
Sensory Structures ◽  
Lattice Organs ◽  
Almost All ◽  
Scanning Electron

The morphology and disposition of lattice organs, putative sensory structures in the carapace of cyprids, are described with scanning electron microscopy from six rhizocephalan species representing four of the five families in the suborder Akentrogonida: Clistosaccus paguri and Sylon hippolytes (Clistosaccidae), Arcturosaccus kussakini (Duplorbidae), Mycetomorpha vancouverensis (Mycetomorphidae), and Diplothylacus sinensis and Thylacoplethus reinhardi (Thompsoniidae). The disposition of pores and setae on the cyprid carapace is also surveyed. In T. reinhardi, poor quality of the specimens allowed only confirmation that lattice organs are present. Cyprids of all other species except M. vancouverensis have two anterior and three posterior pairs of lattice organs on the carapace, just as in almost all other Cirripedia. Cyprids of C. paguri and S. hippolytes are nearly identical in both general shape, carapace setation, and morphology of the lattice organs. In both species the lattice organs are oval–circular pore fields, except the second pair which is very elongate and match-shaped, a morphology unique to the Clistosaccidae. The similarities in cyprid morphology are interpreted as apomorphies supporting the monophyly of the recently rediagnosed Clistosaccidae. Both thompsoniid species have oval–circular lattice organs, indicating a possible sister-group relationship between the Clistosaccidae and the Thompsoniidae. None of the lattice organs of A. kussakini are oval or match-shaped. In M. vancouverensis there is only a single anterior pair of lattice organs, a probable autapomorphy. But the morphology of the M. vancouverensis cyprid is otherwise very reminiscent of those in the Rhizocephala suborder Kentrogonida, such as in being ornamented with numerous (> 100) carapace setae in no fixed pattern compared with the few (< 80) setae in a fixed disposition seen in the other akentrogonids. In cyprids of all Akentrogonida examined, the lattice organs lack a large terminal pore. By out-group comparison with all other cirripedes and with the Ascothoracida, this is regarded as an apomorphy that supports the monophyly of the Akentrogonida.

scholarly journals Study of selection and purification of Brazilian bentonite clay by elutriation: a XRF, SEM and Rietveld analysis

Cerâmica ◽  
2016 ◽  
Vol 62 (361) ◽  
pp. 1-8 ◽  
Author(s):  
J. L. Alves ◽  
A. E. Zanini ◽  
M. E. de Souza ◽  
M. L. F. Nascimento
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Rietveld Method ◽  
Bentonite Clay ◽  
Rietveld Analysis ◽  
Industrial Applications ◽  
X Ray Diffraction ◽  
X Ray ◽  
Almost All ◽  
Scanning Electron

Abstract Clays obtained from nature have a lot of impurities. Therefore, for best using of these materials, it is necessary its selection and purification. Thus, the aim of this work is to separate and to purify the smectite fractions using water as a solvent at a low flux mixed with a bentonite clay extracted from a mine in Vitória da Conquista - Bahia / Brazil. For this a separation method of fractions of expandable clays based on the Stokes' Law was applied - this process is called elutriation, in order to ensure and to expand possible industrial applications of this material. The samples were characterized by analysis of X-ray diffraction, X-ray fluorescence and scanning electron microscopy. The Rietveld method enabled the quantification of main phase minerals: montmorillonite, kaolinite, nontronite and quartz, reaching 85% in mass of montmorillonite phase at the end of the process. Results showed that the method used was efficient to remove almost all quartz, carbonates and organic matter from the sample. It was also observed a monomodal grain size distribution of elutriated materials with thinner grains, around (18.1 ± 1.8) μm at the end of the process. It has been concluded that the method developed and applied showed promising characters to be applied to elutriate kilograms of clays and could be used in industrial scale.

scholarly journals Sexual dichroism and pigment localization in the wing scales of Pieris rapae butterflies

2006 ◽  
Vol 274 (1606) ◽  
pp. 97-102 ◽  
Author(s):  
M.A Giraldo ◽  
D.G Stavenga
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pieris Rapae ◽  
Reflectance Spectra ◽  
High Concentration ◽  
Pieris Rapae Crucivora ◽  
Males And Females ◽  
Wing Coloration ◽  
Scanning Electron ◽  
Wing Scales

The beads in the wing scales of pierid butterflies play a crucially important role in wing coloration as shown by spectrophotometry and scanning electron microscopy (SEM). The beads contain pterin pigments, which in Pieris rapae absorb predominantly in the ultraviolet (UV). SEM demonstrates that in the European subspecies Pieris rapae rapae , both males and females have dorsal wing scales with a high concentration of beads. In the Japanese subspecies Pieris rapae crucivora , however, only the males have dorsal wing scales studded with beads, and the dorsal scales of females lack beads. Microspectrophotometry of single scales without beads yields reflectance spectra that increase slightly and monotonically with wavelength. With beads, the reflectance is strongly reduced in the UV and enhanced at the longer wavelengths. By stacking several layers of beaded scales, pierid butterflies achieve strong colour contrasts, which are not realized in the dorsal wings of female P. r. crucivora . Consequently, P. r. crucivora exhibits a strong sexual dichroism that is absent in P. r. rapae .

scholarly journals Detailed morphological description of Habronema clarki Foster & Chitwood, 1937, a nematode parasite of capybaras Hydrochoerus hydrochaeris (Linnaeus, 1766) in Brazil

2014 ◽  
Vol 23 (2) ◽  
pp. 251-254
Author(s):  
Estevam Guilherme Lux Hoppe ◽  
José Hairton Tebaldi ◽  
Daniel Fontana Ferreira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nematode Parasite ◽  
Morphological Data ◽  
Valid Species ◽  
Morphological Description ◽  
Hydrochoerus Hydrochaeris ◽  
Scanning Electron

The genus Habronema has four valid species, of which only two are properly known. The present study aimed to describe in detail the morphology of Habronema clarki through optical and scanning electron microscopy analyses. Our results showed that the labial morphology of this parasite is closer to H. muscae than to H. microstoma. Even so, the characteristic pseudolabia and the slightly convex border of the dorsal and ventral lips are sufficient to distinguish these nematodes. Additional morphological data are presented, thus contributing to the knowledge on this little known nematode. In addition, this study provides new locality records for this species.

scholarly journals First report ofEucoleus boehmi(syn.Capillaria boehmi) in dogs in north-western Italy, with scanning electron microscopy of the eggs

Parasite ◽  
2012 ◽  
Vol 19 (4) ◽  
pp. 433-435 ◽  
Author(s):  
M. Magi ◽  
L. Guardone ◽  
M.C. Prati ◽  
B. Torracca ◽  
F. Macchioni
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
First Report ◽  
North Western ◽  
Scanning Electron

scholarly journals Anisakid larva parasitizing Plagioscion squamosissimus in Marajó Bay and Tapajós River, state of Pará, Brazil

2016 ◽  
Vol 25 (4) ◽  
pp. 492-496 ◽  
Author(s):  
Gabrielle Fontenelle ◽  
Marcelo Knoff ◽  
Nilza Nunes Felizardo ◽  
Eduardo José Lopes Torres ◽  
Edilson Rodrigues Matos ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Optical Microscopy ◽  
Infection Intensity ◽  
First Record ◽  
South American ◽  
Taxonomic Identification ◽  
Infection Site ◽  
Anisakid Nematodes ◽  
Scanning Electron

Abstract In November 2014 and May 2015, a total of 44 specimens of the South American silver croaker Plagioscion squamosissimus were collected: 30 in Marajó Bay and 14 in the Tapajós River, state of Pará, Brazil. The aim was to investigate the presence of anisakid nematodes and determine their parasitism indices and sites of infection, because of their importance regarding health inspection. Sixty-nine Anisakis sp. larvae were found; among them, 16 larvae in seven fish collected in Marajó Bay and 53 larvae in four fish in the Tapajós River. The parasitism indices of the nematodes collected from the fish in Marajó Bay comprised prevalence (P) of 23%, mean infection intensity (MI) of 2.28, mean abundance (MA) of 0.53, range of infection (RI) of 1-4, and infection site (IS) in the mesentery. The fish from the Tapajós River showed P = 28%, MI = 13.2, MA = 3.8, RI = 1-22, and IS = mesentery and intestine. To assist in taxonomic identification, images of the specimens obtained through optical microscopy with Nomarski's interferential contrast system and scanning electron microscopy were used. This is the first record of Anisakis sp. parasitizing P. squamosissimus.

scholarly journals Contracaecumovale (Nematoda: Anisakidae) from Rollandia rolland Quoy & Gaimard 1824 (Aves, Podicipedidae) in Argentina

2012 ◽  
Vol 21 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Noelia Adelina Galeano ◽  
Ruben Daniel Tanzola
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Morphological Study ◽  
Buenos Aires ◽  
First Record ◽  
Buenos Aires Province ◽  
Marked Specificity ◽  
Scanning Electron

Necropsy on 15 specimens of white-tufted grebe, Rollandiarolland, caught in the Mar Chiquita and Chascomús lagoons (Buenos Aires province), revealed the presence of Contracaecumovale (Linstow, 1907). This nematode shows a marked specificity for podicipediform birds. The specimens were identified from morphological study on features such as cephalic and esophageal structures and caudal papillae, using both optical and scanning electron microscopy. This is the first record of C. ovale parasitizing R. rolland in Argentina.

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