Molecular detection and prevalence of feline hemotropic mycoplasmas in Istanbul, Turkey

2016 ◽  
Vol 61 (1) ◽  
Author(s):  
Handan Cetinkaya ◽  
Damla Haktanir ◽  
Seckin Arun ◽  
Cem Vurusaner
Keyword(s):  
Molecular Detection ◽  
Fragment Length Polymorphism ◽  
Common Species ◽  
Mycoplasma Species ◽  
Pcr Assay ◽  
Blood Samples ◽  
Total Prevalence ◽  
Polymerase Chain ◽  
First Time ◽  
Candidatus Mycoplasma Turicensis

AbstractThe aim of this study was to investigate Mycoplasma spp. species in blood samples of the domestic cats from the province of Istanbul, Turkey. Three hundred eighty four blood samples of client-owned cats were used for the identification of Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm) and Candidatus Mycoplasma turicensis (CMt) by Polymerase Chain Reaction (PCR) and Restriction Fragment Length Polymorphism (RFLP) assays. Out of 384 blood samples, 74 (19.3%) were positive for one of Mycoplasma species. The total prevalence of Mhf, CMhm and CMt infections was 9.9%, 17.7% and 0.8% respectively. The most common species was CMhm. Co-infections were mostly with Mhf/CMhm and the frequency was 8.1%. Two cats were infected with three species. The current study was the first molecular prevalence study of hemotropic mycoplasmas in Istanbul, reporting the presence of CMt for the first time in Turkey. Prevalence of feline mycoplasma was notably high in Istanbul and PCR assay could be preferred rather than the microscopic examination for the diagnosis.

scholarly journals Haemotropic Mycoplasma species in pet cats in Latvia: a study, phylogenetic analysis and clinical case report

2021 ◽  
Vol 7 (2) ◽  
pp. 205511692110280
Author(s):  
Inese Berzina ◽  
Valentina Capligina ◽  
Agne Namina ◽  
Alina Visocka ◽  
Renate Ranka
Keyword(s):  
Phylogenetic Analysis ◽  
Clinical Case ◽  
Mycoplasma Species ◽  
Baltic Region ◽  
Blood Samples ◽  
Northern European ◽  
Published Report ◽  
The Baltic ◽  
First Time ◽  
Candidatus Mycoplasma Turicensis

Objectives The aim of this study was to evaluate whether haemotropic Mycoplasma species are detected in pet cats in Latvia, to perform a phylogenetic analysis of the detected pathogens and to report a clinical case of feline infectious anaemia. Methods Peripheral blood samples (n = 125) from pet cats were submitted; 99 samples were adequate to test for the presence of Mycoplasma species DNA by nested PCR. A clinical case was added in the later stages of the study. Positive isolates were subjected to phylogenetic analysis. Results The prevalence of ‘ Candidatus Mycoplasma haemominutum’ was 15% (n = 15/99), that of Mycoplasma haemofelis was 5% (5/99) and that of ‘ Candidatus Mycoplasma turicensis’ was 2% (n = 2/99). Cases of coinfection included ‘ Candidatus M haemominutum’ + M haemofelis (4%; n = 4/99) and ‘ Candidatus M haemominutum’ + ‘ Candidatus M turicensis’ (1%; n = 1/99). This is the first published report of M haemofelis infection in the Baltic states. Two different ‘ Candidatus M turicensis’ isolates were discovered after phylogenetic analysis. Conclusions and relevance This report is the first of an autochthonous feline infectious anaemia case in the Baltic region. The prevalence of Mycoplasma species was similar to that in other northern European countries. Phylogenetic analysis revealed variability of the isolates; one of the ‘ Candidatus M turicensis’ genotypes was detected for the first time in Europe.

scholarly journals Molecular detection and occurrence of equine theileriosis in Arabian horses in Al-Najaf province/Iraq

2020 ◽  
Vol 57 (3) ◽  
pp. e166996
Author(s):  
Hayder Mohammad Al-Rammahi ◽  
Abdulameer Abed Hatem ◽  
Asaad Chasib Al-Atabi
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Extraction ◽  
Molecular Detection ◽  
Molecular Technique ◽  
Chain Reaction ◽  
Blood Samples ◽  
Equine Piroplasmosis ◽  
Polymerase Chain

This study was designed to detect equine piroplasmosis using the molecular technique in Al-Najaf province during the season that showed an increment in tick activities. Blood samples were collected from 110 horses with more than two signs of piroplasmosis. After DNA extraction, the product was examined by a polymerase chain reaction to amplify 18SrRNA. The results showed that the overall percentage of equine theileriosis was 38.18%. According to gender, the percentage of infection was 43.48% and 29.27% in females and males, respectively. Significant variations appeared between infected horses according to age, and the percentage of infection was 50% and 35.22% in less than 2 years and more than 2 years age, respectively. Moreover, the percentage of infection was 62.5% and 19.35% in animals with and without acariasis, respectively. Significant variations were also seen in equine theileriosis according to geographical areas, and the higher percentage was reported in Hera district (60.87%), while the lowest percentage was in the center of Al-Najaf (21.43%). This difference may be due to different distribution of vector of disease (tick), which may be the availability of the suitable weather that helped in the multiplication of the intermediate vectors. In conclusion, this study proved the variations in the occurrences of equine piroplasmosis according to gender, age, and geographical areas.

scholarly journals Molecular detection of bovine hemotrophic mycoplasmas in Mexico

2020 ◽  
Vol 13 (52) ◽  
pp. 67-82
Author(s):  
Rosa Estela Quiroz Castañeda ◽  
Kytzya Mejía Aragón ◽  
Hugo Aguilar Diaz ◽  
Jesús Francisco Preciado de la Torre
Keyword(s):  
Molecular Detection ◽  
Detection Method ◽  
Animal Health ◽  
Clinical Signs ◽  
Health Condition ◽  
Duplex Pcr ◽  
Blood Samples ◽  
End Point ◽  
Mycoplasma Wenyonii ◽  
First Time

The presence of hemoplasmas Candidatus Mycoplasma haemobos and Mycoplasma wenyonii that infect bovine cattle has been reported during the last years. Hemoplasmas may affect animal health either alone or in coinfections with other microorganisms, resulting in anemia and other clinical signs. In Mexico, only Ca. M. haemobos has been detected in cattle; in this work, we report for the first time in our country the presence of M. wenyonii in animals from different geographical sources amd we detected both hemoplasmas by duplex PCR. Also, by single end-point PCR, we found Ca. M. haemobos and M. wenyonii in 96% and 96.29% of the blood samples, respectively. Both hemoplasmas were detected in 50% of the samples analyzed, which suggest that the duplex PCR developed in this work might improve if some modifications are performed. This molecular detection method will provide valuable information to know the health condition of national cattle to prevent pathogen dispersion.

scholarly journals Molecular detection of Babesia bovis in cattle in Al-Qadisiyah province

2017 ◽  
Vol 40 (2) ◽  
pp. 155-158
Author(s):  
Noaman N. A,aiz
Keyword(s):  
Polymerase Chain Reaction ◽  
Infection Rate ◽  
Molecular Detection ◽  
Babesia Bovis ◽  
Chain Reaction ◽  
Adult Cattle ◽  
Blood Samples ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Age And Sex

     This study aim to determine Babesia bovis infection in cattle based on genetic methods. A total of 96 blood samples were collected from alive and slaughtered cattle from different areas in addition to the abattoir of Al-Qadisiyah province from December 2013 to August 2014. Real time polymerase chain reaction (RT.PCR) technique was used to detect the presence of the protozoan with the effect of animal's age and sex in the infection rate 47.91 % (46/96) of examined cattle were given positive result to B. bovis infection. The highest infections were shown among the adult cattle (≥1 year), while there was non-significant difference (P>0.05) in the infection rate according to the sex. So the most cattle in Al-Qadisiyah province appear to be bearing the infection predominantly as a carrier hosts.

scholarly journals IDENTIFICATION OF FUNCTIONAL SNPs OF MDR1 GENE AMONG NEPHROTIC SYNDROME CHILDREN IN SOUTH INDIA

2017 ◽  
Vol 10 (2) ◽  
pp. 418
Author(s):  
Arumugam Ganesan
Keyword(s):  
Polymerase Chain Reaction ◽  
Nephrotic Syndrome ◽  
Gene Polymorphisms ◽  
Fragment Length Polymorphism ◽  
Chain Reaction ◽  
Blood Samples ◽  
Steroid Sensitive ◽  
Polymerase Chain ◽  
Mdr 1 ◽  
Restriction Fragment Length

Objective: This study was conducted to determine the frequency of C3435T and G2677T/C single nucleotide polymorphisms of multi drug resistancegene -1 (MDR1) in nephrotic syndrome (NS) children in relation to healthy subjects. The role and association of these SNPs were also determined,whether response and/or resistance to steroid treatment in children with NS in south India.Methods: Genomic DNA was isolated from 371 blood samples collected from children with NS and controls. Among 173 cases, categorized intosteroid-resistant NS (SRNS) were 90 and steroid-sensitive NS (SSNS) were 83 and 198 blood samples were included as controls. All samples weresubjected to DNA extraction, and polymerase chain reaction followed by restriction fragment length polymorphism for identification of C3435T andG2677T/C genomic variations.Results: The frequencies of MDR-1 C3435T, CT, TT, and CC genotypes and SNP G2677T/C GG and G allele genotypes were observed in this study group.In SRNS, children showed significantly higher frequencies of MDR-1 C3435T, CT, TT, and TT+CC genotypes were observed than SRNS and controls.The allele frequencies of SRNS children showed CC - 4%, CT - 32% and TT - 12% and in SSNS children, CC - 10.98%, CT - 27.2% and TT - 13.9% wereobserved. Furthermore, increased frequencies of MDR-1 C3435T CT, TT, TT+CC genotypes, or T allele were observed in children aged <9 years old.There were no different genotype and allele frequency observed in G2677T/C genotypes NS children and controls.Conclusion: Based on these data, we are suggesting that MDR-1 C3435T gene polymorphisms are risk factors of increased susceptibility, earlieronset of NS as well as leads to steroid resistance. Whereas SNP G2677T/C gene polymorphisms do not have significant role observed in this studypopulation.Keywords: Nephrotic syndrome, Steroid-sensitive nephrotic syndrome, Steroid-resistant nephrotic syndrome, Multidrug resistance gene-1,Polymerase chain reaction followed by restriction fragment length polymorphism.

scholarly journals Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction

2014 ◽  
Vol 58 (4) ◽  
pp. 527-531
Author(s):  
Faham Khamesipour ◽  
Abbas Doosti ◽  
Mohsen Fard Emadi ◽  
Babafela Awosile
Keyword(s):  
Polymerase Chain Reaction ◽  
Age Groups ◽  
Conventional Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Blood Samples ◽  
Stray Dogs ◽  
Supplementary Method ◽  
Significant Difference ◽  
Polymerase Chain ◽  
First Time

Abstract The study was conducted to detect Brucella sp. and Leptospira sp. in blood samples of dogs in Isfahan and Shahrekord province in Iran. A total of 94 blood samples were collected from dogs of different breed, age, sex, and dogs’ type (stray or nonstray). The samples were examined using conventional polymerase chain reaction (PCR). Fourteen (14.89%) dogs were positive for Brucella sp. and 18 (19.15%). dogs for Leptospira sp. There were no significant differences between the prevalence of the pathogens, provinces, sex, and age groups (P > 0.05). However, there was a statistically significant difference in prevalence of Brucella sp. and Leptospira sp. between stray and non-stray dogs (P < 0.0001; χ2 = 30.3767). The study also demonstrated that PCR was successfully used for the first time in Iran for the detection of Brucella sp. and Leptospira sp. in blood samples of dogs. Therefore, we recommend the PCR as a supplementary method with other commonly recognised methods (e.g. serological methods) for the diagnosis of subclinical infections with the microorganisms. Strict measures for the control of stray dogs are also highly recommended.

scholarly journals Molecular Species Identification of Candida from Blood Samples of Intensive Care Unit Patients by Polymerase Chain Reaction – Restricted Fragment Length Polymorphism

2012 ◽  
Vol 4 (01) ◽  
pp. 001-004 ◽  
Author(s):  
Ramraj Vijayakumar ◽  
Sidhartha Giri ◽  
Anupma Jyoti Kindo
Keyword(s):  
Intensive Care Unit ◽  
Polymerase Chain Reaction ◽  
Intensive Care ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Chain Reaction ◽  
Candida Spp ◽  
Blood Samples ◽  
Pcr Rflp ◽  
Polymerase Chain

ABSTRACT Introduction: Candida spp is an emerging cause of blood stream infections worldwide. Delay in speciation of Candida isolates by conventional methods and resistance to antifungal drugs (especially fluconazole, amphotericin B, etc.) in various Candida species are some of the factors responsible for the increase in morbidity and mortality due to candidemia. So, the rapid detection and identification of Candida isolates from blood is very important for the proper management of patients having candidemia. Materials and Methods: In this study, we have used polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) as a method for the speciation of Candida isolates from blood samples of intensive care unit (ICU) patients. PCR was used to amplify the ITS-1 and ITS-2 regions of Candida spp using universal primers ITS-1 and ITS-4. The amplified product was digested using Msp I restriction enzyme by RFLP. Results and Discussion: The method PCR-RFLP helped in identifying five medically important Candida spp (C. tropicalis, C. albicans, C. parapsilosis, C. krusei and C. glabrata) from blood. This method is rapid, reliable, easy and cost-effective and can be used in routine laboratory diagnostics for the rapid identification of Candida isolates from blood. Conclusion: PCR-RFLP is an easy, rapid and highly valuable tool which can be used in routine diagnostic laboratories to speciate Candida isolates obtained from blood. This rapid method of speciation will help clinicians to decide on empirical therapy in candidemia cases before antifungal susceptibility results are available.

scholarly journals Profile of follicle-stimulating hormone and polymorphism of follicle-stimulating hormone receptor in Madrasin cattle with ovarian hypofunction

2020 ◽  
Vol 13 (5) ◽  
pp. 879-883
Author(s):  
Budi Utomo ◽  
Emmanuel Djoko Putranto ◽  
Amaq Fadholly
Keyword(s):  
Fragment Length Polymorphism ◽  
Follicle Stimulating Hormone ◽  
Restriction Enzymes ◽  
Chain Reaction ◽  
Blood Samples ◽  
Fshr Gene ◽  
Polymerase Chain ◽  
Genetic Makeup ◽  
Restriction Fragment Length ◽  
Stimulating Hormone

Background and Aim: The follicle-stimulating hormone (FSH) gene is an essential regulator of fertility in livestock. This study aims to provide information on the genetic makeup of Madrasin cattle experiencing hypofunction by the FSH profile and FSH receptors (FSHR) polymorphism. Materials and Methods: Blood samples were collected from the Bangkalan regency in Indonesia. DNA was isolated and purified following the extraction protocol of polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. Results: Our results showed that the FSH gene had a band length of 310 bp and produce two alleles (A and B) with restriction enzymes at 250 bp, 230 bp, and 145 bp. Furthermore, the FSHR gene had a band length of 303 bp and produced two homozygous genotypes: GG at bp 239 and CC at bp 188. Conclusion: Based on these differences, there was no change in allele frequency and genotype between Madura and Madrasin cattle due to crossbreeding with Limousin cattle. Thus, further detailed investigations of Madrasin cattle are required to elucidate the profile of the LH and LHR genes.

scholarly journals Detection of Mycoplasma agalactiae by Polymerase Chain Reaction in Jordanian Sheep and Goat Herds

2007 ◽  
Vol 76 (1) ◽  
pp. 71-77 ◽  
Author(s):  
D. Zendulková ◽  
A. Madanat ◽  
P. Lány ◽  
K. Rosenbergová ◽  
Z. Pospíšil
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Signs ◽  
Mycoplasma Species ◽  
Sample Collection ◽  
Chain Reaction ◽  
Mycoplasma Agalactiae ◽  
Sheep And Goats ◽  
Contagious Agalactia ◽  
Polymerase Chain ◽  
First Time

The aim of the study was to ascertain whether sheep and goats from selected Jordanian herds were infected with Mycoplasma agalactiae, the most common aetiological agent of contagious agalactia of sheep and goats. All examined animals showed clinical signs of disease at the time of sample collection. The group included 35 animals, 15 sheep and 20 goats. For microbiological examination, a total of 107 swabs were taken from conjunctival, nasal, vaginal or preputial mucosae and from the external auditory canal. Identification of the species isolated was carried out by a polymerase chain reaction. Of the 35 animals, 21 (4 sheep and 17 goats) tested positive for Mycoplasma agalactiae. These results confirmed our assumption that this mycoplasma species is present in Jordanian herds and, for the first time, provided evidence that contagious agalactia of sheep and goats occurs in Jordan.

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