Comparison of selenium-induced antioxidant responses and bioaccumulation in two strains of the halotolerant alga Dunaliella salina

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Aida Hamidkhani ◽  
Ezat Asgarani ◽  
Azra Saboora ◽  
Mohammad Amin Hejazi
Keyword(s):  
Industrial Wastewater ◽  
Dunaliella Salina ◽  
High Efficiency ◽  
High Salinity ◽  
Selenium Concentration ◽  
Cell Number ◽  
Hypersaline Environments ◽  
Antioxidant Responses ◽  
Primary Producer ◽  
High Selenium

Abstract Selenium water pollution is an increasing environmental problem that requires investigation of cellular responses of aquatic primary producer organisms, especially algae. Industrial wastewater with selenium contamination is often coupled with high salinity (60–70). In this study, the biochemical responses of two strains of the halotolerant alga (Dunaliella salina Hoze-soltan and Dunaliella salina CCAP 19/18) to different selenium concentrations were evaluated. Although at high selenium concentrations both strains showed lipid peroxidation and cell number reduction, Dunaliella salina Hoze-soltan was less affected. Higher selenium tolerance in this strain might be attributed to the better activity of resistance responses like proline, total reducing sugar, superoxide dismutase (SOD) and peroxidase (POX), even at the high selenium concentrations. Catalase (CAT) had no significant role for protection against selenium toxicity as its activity declined in both strains with rising selenium concentration. Both strains accumulated selenium intracellularly, but the accumulation was about three-fold higher in Dunaliella salina Hoze-soltan than in the other strain. It can be concluded that Dunaliella salina Hoze-soltan may be a better candidate for selenium bioremediation of a high salinity environment. The data obtained from this study could be useful for improvement of algal ability for high efficiency selenium bioremediation in hypersaline environments.

scholarly journals Tolerance of microalgae Dunaliella salina and Phaeodactylum tricornutum to various concentrations of selenium in the medium

2018 ◽  
Vol 195 ◽  
pp. 201-208
Author(s):  
N. A. Aizdaicher ◽  
Zh. V. Markina
Keyword(s):  
Growth Rate ◽  
Phaeodactylum Tricornutum ◽  
Dunaliella Salina ◽  
Control Sample ◽  
Selenium Concentration ◽  
Cell Number ◽  
Number Dynamics

Cell number dynamics and growth rate of microalgaeDunaliella salina(Chlorophyta) andPhaeodactylum tricornutum(Bacillariophyta) are considered in dependence on selenium concentration in the medium. The concentrations 0.01, 0.10, and 0,50 mg/l were tested. Onetime addition of selenium did not influence on the cell number and morphology ofD. salina. Under repeatable addition of selenium (every 2 days), reliable differences in the cells number were detected for the concentration 0.10 mg/l only, but the concentrations 0.01 and 0.50 mg/l did not make any effect on the cells different from the control. Under frequent repeatable addition of selenium (every day), the concentration 0.50 mg/l caused a visible decreasing of the cell number in compare with the control sample — after 6 days the cells started to decay, settled to the flask bottom and aggregated, the suspension color turned to yellowish. Reaction of diatomPhaeodactylum tricornurumwas more complicated. Its cell number increased signifcantly against the control under both one-time addition of selenium in the concentration of 0.10 and 0.50 mg/l or the repeatable addition in every 2 days in the concentration 0.01 and 0.10 mg/l. But more frequent (every day) addition of selenium in the concentration 0.50 mg/l caused inhibition of the cells growth and decreasing of their number against the control sample — after 4 days the cells adhesion to the flask bottom was detected, apparently because of their metabolism alteration.

scholarly journals Molecular and phylogenetic analysis reveals new diversity of Dunaliella salina from hypersaline environments

2021 ◽  
pp. 1-11
Author(s):  
Andrea Highfield ◽  
Angela Ward ◽  
Richard Pipe ◽  
Declan C. Schroeder
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Dunaliella Salina ◽  
Phylogenetic Analyses ◽  
Ssu Rdna ◽  
Lsu Rdna ◽  
Hypersaline Environments ◽  
Rdna Its ◽  
Β Carotene ◽  
Selection Of

Abstract Twelve hyper-β carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing of the SSU rDNA and phylogenetic analysis of these isolates were undertaken using four commonly employed markers for genotyping, LSU rDNA, ITS, rbcL and tufA and their application to the study of Dunaliella evaluated. Novel isolates have been identified and phylogenetic analyses have shown the need for clarification on the taxonomy of Dunaliella salina. We propose the division of D. salina into four sub-clades as defined by a robust phylogeny based on the concatenation of four genes. This study further demonstrates the considerable genetic diversity within D. salina and the potential of genetic analyses for aiding in the selection of prospective economically important strains.

Study on the Treatment of Nylon Chemical Industrial Wastewater by AF+ A/O Process

2011 ◽  
Vol 71-78 ◽  
pp. 3133-3136
Author(s):  
Jie Zhang ◽  
Qun Hui Wang ◽  
Peng Li ◽  
Hai Ming Huang ◽  
Wen Jun Wang ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Treatment Effect ◽  
Industrial Wastewater ◽  
High Efficiency ◽  
First Grade ◽  
Wastewater Discharge ◽  
N Removal ◽  
New Type ◽  
Nitrogen Treatment ◽  
A Company

A company in Pingdingshan of Henan province using a new type of AF (high-efficiency immobilized biological anaerobic biofilter ) and A/O process to treat nylon chemical industrial wastewater, In this study, we monitored the system for one month (Dec.1.2010-Jan.1.2011). The effect of the conditions of temperature, pH and COD, nitrate, ammonia and total nitrogen treatment effect along the system were investigated, the results showed that: average COD removal efficiency reached 97%, average NH4+-N removal efficiency was higher than 90% and the effect of the system is good. The effluent meets the first grade criteria of integrated wastewater discharge standard (GB8978-1996).

scholarly journals MORPHOLOGICAL AND MORPHOMETRICAL FEATURES IN DUNALIELLA SALINA (CHLAMYDOMONADALES, DUNALIELLACEAE) DURING THE TWO-PHASE CULTIVATION MODE

2019 ◽  
Vol 22 ◽  
pp. 157-165
Author(s):  
Andrei B. Borovkov ◽  
Irina N. Gudvilovich ◽  
Olga A. Memetshaeva ◽  
Anna L. Avsiyan ◽  
Alexander S. Lelekov ◽  
...  
Keyword(s):  
Cell Volume ◽  
Dunaliella Salina ◽  
Linear Growth ◽  
Cell Number ◽  
Two Phase ◽  
Cell Content ◽  
Crimean Peninsula ◽  
Cell Parameters ◽  
Maximum Productivity ◽  
Salina Cell

The paper presents studies of morphological and morphometrical characteristics of green halophilic carotenogenic microalga Dunaliella salina (Dunal) Teodoresco, 1905, from the south-west region of Crimean Peninsula. D. salina was cultivated in two-phase mode under conditions of natural illumination at the premises of A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS (IBSS), Sevastopol, Russia. The maximum D. salina cell density was 1.69·106 cell/ml in the “green” phase and 0.84·106 cell/ml in the “red” growth phase. The maximum productivity by cell number reached 0.15 ∙ 106 cell/(ml·day)in the “green” phase while it was lower by 73% in the “red” phase (0.04∙106 cell/(ml·day). Along with the maximum productivity, linear growth stage in the first phase was characterized by a maximum fraction of small (up to 500 μm3 in volume) cells (about 15-29%) and a decrease in cell volume by 40-45% as compared with initial value. The mean of D. salina cell volume in the “red” phase was 30% higher than in the “green” phase. At the same time, the large cell fraction in the “red” phase was consistently high (15-35%). The patterns of change in morphological and morphometrical cell parameters were in accordance to stage and conditions of growth. Thus, cell elongation was noted in the stage of linear growth, while under unfavorable conditions at growth-declining stage cells became more round-shaped, with orange and tile-red coloration and granulation of cell content. It was shown that morphological and morphometric cell parameters can serve as additional criteria for assessment of physiological condition in D. salina culture. The experiment demonstrated the prospects for two-stage D. salina cultivation in Crimea.

Biological Treatment of a High Salinity Chemical Industrial Wastewater

1993 ◽  
Vol 27 (7-8) ◽  
pp. 105-112 ◽  
Author(s):  
Shimshon Belkin ◽  
Asher Brenner ◽  
Aharon Abeliovich
Keyword(s):  
Activated Carbon ◽  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Salt Concentration ◽  
Industrial Wastewater ◽  
Biological Treatment ◽  
High Salinity ◽  
Laboratory Bench ◽  
Wastewater Stream ◽  
Scale Reactor

Various laboratory-scale process configurations were tested for the biological treatment of a combined wastewater stream of several chemical factories. The untreated wastewaters, rich in halogenated organics (1250±389 mg/l DOC), were also highly saline (32±11 g/liter TDS 550°C) and toxic (Microtox™ EC50 = 1.5±2.0%). Biphasic (anaerobic/aerobic) laboratory bench-scale reactor systems yielded reduction of dissolved organic carbon by 70 to 84%, in the absence and presence of powdered activated carbon, respectively. The anaerobic phase proved to be essential in all systems, both for dissolved organic carbon removal and for detoxification. Similar efficiencies were obtained in either activated sludge or aerated lagoon type reactors, but in the latter case, longer hydraulic retention times were required. DOC removal was found to decrease with increased salt concentration; however, a 50% efficiency was achieved even at 90 g/l TDS. Toxicity elimination as judged by the Microtox™ assay was highly variable in the absence of activated carbon but stable and efficient in its presence.

scholarly journals Genomic adaptations of the green alga Dunaliella salina to life under high salinity

2020 ◽  
Vol 50 ◽  
pp. 101990 ◽  
Author(s):  
Jürgen E.W. Polle ◽  
Sara Calhoun ◽  
Zaid McKie-Krisberg ◽  
Simon Prochnik ◽  
Peter Neofotis ◽  
...  
Keyword(s):  
Green Alga ◽  
Dunaliella Salina ◽  
High Salinity

scholarly journals Impact of environmental conditions on photosynthesis, growth and carbon allocation strategies of hypersaline species of Dunaliella

2013 ◽  
Vol 11 (1) ◽  
pp. 79-85
Keyword(s):  
Resource Utilization ◽  
Environmental Conditions ◽  
Metabolic Pathways ◽  
Inorganic Carbon ◽  
High Salinity ◽  
Carbon Allocation ◽  
Inorganic Ions ◽  
High Light ◽  
Hypersaline Environments ◽  
Allocation Strategies

Hypersaline environments pose a number of ecological and metabolic challenges to the organisms that live in them. Primary producers, such as halotolerant species of the green microalgal genus Dunaliella, are no exception. In this paper we focus on the problems posed to the acquisition and metabolism of inorganic nutrients and on the consequences of exposure to high light and UV radiation. We show that not only does growth in high salinity environments have repercussions on the flow of carbon into osmolytes such as glycerol, it also affects speciation of inorganic carbon and the uptake of inorganic ions by the cells. The strategies that Dunaliella adopt to optimize resource utilization and the interactions among metabolic pathways are also discussed.

scholarly journals Effects of the amplitude and frequency of salinity fluctuations on antioxidant responses in juvenile tongue sole, Cynoglossus semilaevis

2016 ◽  
Vol 14 (2) ◽  
pp. e0503 ◽  
Author(s):  
Sachin O. Khairnar ◽  
Xiangli Tian ◽  
Shuanglin Dong ◽  
Ziheng Fang ◽  
Bhavesh V. Solanki ◽  
...  
Keyword(s):  
High Salinity ◽  
Cynoglossus Semilaevis ◽  
The Body ◽  
Sod Activity ◽  
Antioxidant Responses ◽  
Tongue Sole ◽  
Moderate Salinity ◽  
Cortisol Levels ◽  
And Control ◽  
Body Damage

To understand the tolerance of tongue sole, Cynoglossus semilaevis, to varying salinities, the effects of the amplitude (2, 4, 6 and 8 g/L) and frequency (2, 4 and 8 days) of salinity fluctuations on the activities of antioxidant responses, including acidic phosphatase (ACP), alkaline phosphatase (AKP), catalase (CAT) and superoxide dismutase (SOD) from antioxidant system in liver, muscle, gills and kidney, were investigated in this study. The results showed that the antioxidant responses of tongue sole were highly tissue-specific during the varying salinity fluctuations. In all tissues, ACP and AKP activity was found to be highest at moderate salinity fluctuations compared to the control, low and high salinity treatments (p<0.05). SOD and CAT activities had significant effect due to salinity fluctuations in all tissues (p<0.05), except in hepatic and renal tissues. Variations in branchial SOD activity proved that salinity fluctuations had greater impact on tongue sole at moderate and high fluctuating salinities compared to the control and low fluctuating salinities, whereas the branchial CAT activities showed contrasting trend. Further, cortisol levels were significantly affected in lower and higher salinity fluctuations. However, plasma cortisol levels remained low in moderate salinity fluctuations and control (p<0.05). Taken together, the results indicated that salinity fluctuations could effectively stimulate and enhance the antioxidant enzyme activity in the liver, kidney, gills and muscle of the juvenile tongue sole, thus effectively eliminating the excessive reactive oxygen species and minimizing the body damage in tongue sole or could be for any other euryhaline teleosts.

scholarly journals Possible applications of ferrate(VI) in the treatment of industrial wastewater effluent in the laboratory

2015 ◽  
Vol 69 (1) ◽  
pp. 43-50
Author(s):  
Ljiljana Nikolic-Bujanovic ◽  
Milan Cekerevac ◽  
Milena Tomic ◽  
Mladen Zdravkovic
Keyword(s):  
Industrial Wastewater ◽  
High Efficiency ◽  
Oxygen Demand ◽  
Wastewater Effluent ◽  
High Concentration ◽  
Industrial Plants ◽  
Physical Chemical ◽  
Developed Surface ◽  
High Sorption ◽  
High Chemical Oxygen Demand

This paper shows the effects of ferrate(VI) application in the treatment of industrial wastewater effluent in laboratory conditions. Samples used are a mixture of wastewater effluent from various industrial plants whose composition was determined by analyzing samples before the ferrate(VI) treatment. Determining physical-chemical characteristics of the samples showed very high chemical oxygen demand (COD) and the concentrations of individual pollutants are higher than the maximum allowed. In the tested samples, the COD was from 18 (sample 1) to 580 times (sample 2) greater than allowed (allowed 45 - 150 mg/l), while the concentrations of certain metals were several times higher than allowed (Cu allowed 0,1 mg/l, Zn allowed 0,2 mg/l). Also, a high concentration of total phosphorus content (allowed 2 mg/l) and fluoride was found (allowed 20 mg/l), 2-3 times higher than permissible. The applied Na2FeO4 was synthesized by electrochemical method and applied in situ. Physical-chemical testing of samples, after treatment with different amounts (2, 5, 8, and 10 ml) of Na2FeO4, concentration of 8 g/l showed that ferrate(VI) can be used as a multifunctional agent in the purification of industrial wastewater, where in the amount of contaminating matter is reduced below the maximum permitted level. It was demonstrated the high efficiency of ferrate(VI) as a strong oxidant in the removal of total P and suspended materials. Also pointed out was the high sorption power of the generated ferric(III)hydroxide, which with its developed surface absorbs 95,5% of the F- and removes it from the solution in the form of sludge. Showed a high efficiency of ferrate(VI) in the total removal of P (70 to 99,5%), and F- (89 to 95,5%), but depending on the presence of the total COD value or the presence of the total organic substances. At high values of the COD major part of ferrate(VI) is consumed in the oxidation of organic material and the formation of Fe(OH)3, which accelerate the process of decomposition of ferrate(VI), which contributes to lower the efficiency of the removal of P and F-.

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