In vitro leaves and twigs antimicrobial properties of Psidium guajava L. (Myrtaceae)

Summary The current study aimed to investigate antimicrobial activity of methanol, ethanol, acetone, ethyl acetate and hot water (leaves and twigs fractions) Psidium guajava L. crude extracts against three bacteria and two fungi pathogens. Antimicrobial activity expressed by disc-diffusion assay (zone of inhibitions - ZIs), activity index (AI) and minimum inhibitory concentration (MICs) that were measured as reported in many investigations. Based upon the estimated ZIs, AI and MICs values, hot water twigs <1 cm diameter extract was the most potent against all tested microorganisms. The MICs value ranged between 4 and 7.2 mg/ml for bacteria, while, it was between 14.5 and 37.3 mg/ml for fungi. Moreover, ethyl acetate had the lowest antimicrobial activity compared to the other tested solvents. From the results obtained herein, it could be concluded that P. guajava serve as antibacterial and antifungal agent.

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Screening of antibacterial and antifungal activities of selected Macedonian wild mushrooms

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1324333n ◽

2013 ◽

pp. 333-340 ◽

Cited By ~ 8

Author(s):

Daniela Nikolovska-Nedelkoska ◽

Natalija Atanasova-Pancevska ◽

Haris Amedi ◽

Dafina Veleska ◽

Emilija Ivanova ◽

...

Keyword(s):

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Methanolic Extract ◽

Antimicrobial Properties ◽

Inhibitory Effects ◽

Antibacterial And Antifungal Activities ◽

Microdilution Method ◽

Methanolic Extracts ◽

Antibacterial And Antifungal

Regarding the development of novel safe antimicrobials of natural origin, macrofungi became attractive for the researchers in the last decade. In this study, antimicrobial potential of methanolic extracts of six wild macromycetes (Boletus lupinus, Flammulina velutypes, Phellinus igniarius, Sarcodon imbricatus, Tricholoma aurantium, Xerocomus ichnusanus) was evaluated. In vitro antimicrobial activity was investigated by the microdilution method and minimum inhibitory concentration (MIC) was determined. Testing was conducted against eleven microorganisms, including six strains of bacteria and five species of fungi. Extracts showed selective antimicrobial properties while the activities depended both on the species of microorganism and on the type and concentration of extract. The evaluated extracts demonstrated antimicrobial activity, exhibiting more potent inhibitory effects on the growth of bacteria than on fungi. The highest antibacterial and antifungal activity was observed in methanolic extract of polypore fungus P. igniarius.

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Antimicrobial Activity of Few Medicinal Plants against Clinically Isolated Human Cariogenic Pathogens—An In Vitro Study

ISRN Dentistry ◽

10.5402/2011/541421 ◽

2011 ◽

Vol 2011 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

H. Shyla Jebashree ◽

S. Jayasurya Kingsley ◽

Emmanuel S. Sathish ◽

D. Devapriya

Keyword(s):

Ethyl Acetate ◽

Inhibitory Concentration ◽

Ethyl Acetate Extract ◽

In Vitro Study ◽

Ethanol Extract ◽

Psidium Guajava ◽

Terminalia Chebula ◽

Zone Of Inhibition ◽

Mimusops Elengi

Hexane, ethyl acetate, ethanol and methanol extracts of Psidium guajava, Terminalia chebula, Mimusops elengi and Achyranthes aspera were tested against the dental caries causing bacteria Streptococcus mutans and fungus Candida albicans isolated from caries infected patients. All the four extracts of P. guajava showed activity against both S. mutans and C. albicans. Maximum zone of inhibition was observed in ethyl acetate of P. guajava. The four extracts of T. chebula and M. elengi showed antibacterial activity against S. mutans. M. elengi extracts and ethanol extract of T. chebula did not show any antifungal activity against C. albicans. Except for the hexane extract of A. aspera, the other three extracts showed activity against the tested microbes. The ethyl acetate P. guajava leaf extract showed the minimum inhibitory concentration (MIC) against S. mutans to be <0.076 mg/mL in both MHB and BHI. The P. guajava ethyl acetate extract was subjected to GC-MS.

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In vitro Antimicrobial Activity of Plants used in Cambodian Traditional Medicine

The American Journal of Chinese Medicine ◽

10.1142/s0192415x07005338 ◽

2007 ◽

Vol 35 (05) ◽

pp. 867-873 ◽

Cited By ~ 9

Author(s):

Aun Chea ◽

Marie-Caroline Jonville ◽

Sok-Siya Bun ◽

Michèle Laget ◽

Riad Elias ◽

...

Keyword(s):

Antimicrobial Activity ◽

Traditional Medicine ◽

Bactericidal Effect ◽

Gram Positive Bacteria ◽

Antibacterial And Antifungal Activities ◽

Methanolic Extracts ◽

Disk Diffusion Assay ◽

Antibacterial And Antifungal ◽

Diffusion Assay

The purpose of the present study was to screen 27 plant species used in the traditional medicine of Cambodia for in vitro antibacterial and antifungal activities. Thirty-three methanolic extracts were tested against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Mycobacterium smegmatis and Candida albicans. Screened by disk diffusion assay, the extracts showed antimicrobial activity especially on Gram-positive bacteria. None of the crude methanolic extracts showed activity against P. aeruginosa. Twenty-five selected extracts were evaluated using a micro-dilution test. Harrisonia perforata (roots) and Hymenodictyon excelsum (bark) exhibited a bactericidal effect against S. aureus at a concentration of 500 μg/ml. Azadirachta indica (bark), Harrisonia perforata (roots and stem) and Shorea obtusa (roots) exhibited a bactericidal effect against M. smegmatis at 250 μg/ml.

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Antibacterial and antifungal activity of zinc(II) complexes with some 2-methylbenzimidazole derivatives

Acta periodica technologica ◽

10.2298/apt0536231p ◽

2005 ◽

pp. 231-238 ◽

Cited By ~ 1

Author(s):

Sanja Podunavac-Kuzmanovic ◽

Dragoljub Cvetkovic ◽

Gordana Cetkovic

Keyword(s):

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Complex Structure ◽

Gram Negative Bacteria ◽

Antibacterial And Antifungal Activity ◽

Yeast Growth ◽

In Vitro Antimicrobial Activity ◽

Antibacterial And Antifungal ◽

Candida Pseudotropicalis

Zinc(II) chloride reacts with 2-methylbenzimidazole derivatives to give complexes of the formula ZnL2Cl2-nH2O, where L=2-methylbenzimidazole l-benzyl-2-methylbenzimida-zole and l-(4-methylbenzyl)-2-methylbenzimidazole n=0, 0.5 or 1. All the ligands and their zinc(II) complexes were evaluated for their in vitro antimicrobial activity against Pseudomonas aeruginosa Bacillus sp., Staphylococcus aureus, Sarcina lutea and Candida pseudotropicalis. It was found that the majority of the investigated compounds displayed in vitro antimicrobial activity against very persistent microorganisms, except for the starting ligand, 2-methylbenzimidazole and its zinc(II) complex which were active only against gram-negative bacteria. None of the compounds was significantly effective against Candida pseudotropicalis, except for l-(4-methylbenzyl)-2-methylbenzimidazoleandits complex, which very slightly or slightly inhibited the yeast growth. The minimum inhibitory concentration (MIC) was determined for all the ligands and their complexes. The effect of ligand and complex structure on the antimicrobial activity was discussed.

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Synthesis and Antimicrobial Properties of Naphthylamine Derivatives Having a Thiazolidinone Moiety

Medicina ◽

10.3390/medicina47060047 ◽

2011 ◽

Vol 47 (6) ◽

pp. 47

Author(s):

Vilma Petrikaitė ◽

Eduardas Tarasevičius ◽

Alvydas Pavilonis

Keyword(s):

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Antifungal Activity ◽

Inhibitory Concentration ◽

Antimicrobial Properties ◽

Microbiological Analysis ◽

Antibacterial And Antifungal Activity ◽

Antibacterial And Antifungal ◽

New Compounds

Objective. The aim of this study was to evaluate the influence of pharmacophores having naphthylamine and nitro groups on the antimicrobial (antibacterial and antifungal) activity of thiazolidinone derivatives. Materials and Methods. The initial 5-substituted-2-methylmercaptothiazolidin-4-ones were subjected to S-demethylation to yield 2-amino-substituted thiazolidinones. 4-Nitro-1-naphthylamine, nitrofuran aldehydes, and nitrobenzene aldehydes were used as pharmacophoric compounds having amino or aldehyde groups. Antimicrobial (antibacterial and antifungal) activity of the new compounds was tested in vitro against bacterial cultures – Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Klebsiella pneumoniae – and fungal cultures – Candida albicans, Candida glabrata, Candida krusei, Candida kefyr, Candida tropicalis, and Candida parapsilosis. Results. Microbiological analysis showed that all new thiazolidinone derivatives with nitronaphthylamine substituent possessed antibacterial and antifungal properties. New compounds 2a-b showed similar antibacterial activity in vitro against S. aureus and B. subtilis as aminopenicillins. The lowest antibacterial activity of all newly synthesized compounds was against capsule-forming bacteria K. pneumoniae and against gram-negative bacteria E. coli (minimum inhibitory concentration range, 500–1000 μg/mL). Conclusions. The minimum inhibitory concentration of naphthylamine derivatives varied in the range of 0.4–1000 μg/mL, and activity of some newly synthesized compounds was similar to the activity of aminopenicillins and fluconazole, an antifungal preparation. Based on the results, it is possible to separate the perspective group of potential antimicrobial compounds.

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An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽

10.3390/ma13194383 ◽

2020 ◽

Vol 13 (19) ◽

pp. 4383

Author(s):

Barbara Lapinska ◽

Aleksandra Szram ◽

Beata Zarzycka ◽

Janina Grzegorczyk ◽

Louis Hardan ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Antifungal Activity ◽

Antimicrobial Agents ◽

Resin Composite ◽

Antimicrobial Properties ◽

In Vitro Study ◽

Diffusion Method ◽

Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

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Chemical constituents, in vitro antioxidant and antimicrobial properties of ethyl acetate extract obtained from Cytisus triflorus l’Her

Natural Product Research ◽

10.1080/14786419.2018.1519816 ◽

2018 ◽

Vol 34 (11) ◽

pp. 1586-1590 ◽

Cited By ~ 2

Author(s):

Wassila Benabderrahmane ◽

Amel Amrani ◽

Ouahiba Benaissa ◽

Marta Lores ◽

J. Pablo Lamas ◽

...

Keyword(s):

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Chemical Constituents ◽

Antimicrobial Properties

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Chemical analysis and evaluation of antioxidant and antimicrobial activities of fruit fractions ofMauritia flexuosaL. f. (Arecaceae)

PeerJ ◽

10.7717/peerj.5991 ◽

2018 ◽

Vol 6 ◽

pp. e5991 ◽

Cited By ~ 7

Author(s):

Carla de Fatima Alves Nonato ◽

Débora Odília Duarte Leite ◽

Rafael Caldas Pereira ◽

Aline Augusti Boligon ◽

Jaime Ribeiro-Filho ◽

...

Keyword(s):

Ethyl Acetate ◽

Antimicrobial Properties ◽

Antimicrobial Activities ◽

Antifungal Drugs ◽

Resistant Bacteria ◽

Array Detector ◽

Chloroform Fraction ◽

Standard Samples ◽

Antioxidant And Antimicrobial Activities

BackgroundFruit consumption is currently considered beyond the nutritional aspects because of the important roles in disease prevention and benefits to health. The objective of this study was to characterize the chemical profile and evaluate the antioxidant and antimicrobial properties of different fractions obtained from fruit pulps ofMauritia flexuosa(MFFs).MethodsInitially, chloroform, ethyl acetate and ethanol fractions were obtained from the pulps. Quantifications of total phenols and flavonoids were performed using the methods of Folin-Ciocalteu and complexation with aluminum chloride, respectively. Standard samples were used to identify and quantify phenolic acids and flavonoids using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). The antioxidant capacity of the fractions was verified by sequestration of the free radical 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) and iron chelating activity. The antimicrobial activity was determined using the microdilution method and the modulating activity was determined using sub-inhibitory concentrations of the fractions in association with antibiotics.ResultsThe chemical analyzes revealed the presence of catechin, caffeic acid, rutin, orientin, quercetin, apigenin, luteolin and kaempferol, where all are present in the ethyl acetate fraction. The fractions exhibited moderate antioxidant and antimicrobial activities against Gram-positive andCandidastrains in addition to modulating the activity of conventional antibiotics. The most expressive result was obtained from the association of the chloroform fraction with cefotaxime, which produced a synergistic effect, reducing the minimum inhibitory concentration (MIC) of the antibiotic from 1,024 to 256 μg/mL.DiscussionThe fractions presented a constitution rich in phenolic compounds, especially flavonoids. The data obtained demonstrated that the fractions presented moderate antioxidant activity by acting both as primary and secondary antioxidants. The fractions presented antimicrobial and antibiotic potentiating activities, being the first record of modulating effect of fractions of this species against the studied microbial strains, but failed in modulating the activity of antifungal drugs, indicating that this plant has the potential to be used in the development of therapeutic alternatives against resistant bacteria. The constitution phenolic the fractions may be responsible for their pharmacological properties in vitro.

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In vitro evaluation of the antimicrobial activity of five root canal sealers

Brazilian Dental Journal ◽

10.1590/s0103-64402004000100006 ◽

2004 ◽

Vol 15 (1) ◽

pp. 30-35 ◽

Cited By ~ 23

Author(s):

Brenda Paula Figueiredo de Almeida Gomes ◽

José Assis Pedroso ◽

Rogério Castilho Jacinto ◽

Morgana Eli Vianna ◽

Caio Cezar Randi Ferraz ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Properties ◽

Contact Method ◽

Streptococcus Sanguis ◽

Actinomyces Naeslundii ◽

Agar Diffusion Test ◽

Significant Difference ◽

Ah Plus ◽

Endodontic Sealers

The aim of the present study was to analyze the antimicrobial properties of five endodontic sealers: Endo Fill, Endomethasone, Endomethasone N, Sealer 26 and AH-Plus, against the following microorganisms: Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Streptococcus sanguis and Actinomyces naeslundii. The sealers were tested immediately, 24 h, 48 h and 7 days after manipulation.The direct contact method through the observation of the microbial growth in liquid medium and the agar diffusion test were used to evaluate the antimicrobial properties of the sealers. The results, in both methodologies used, showed that immediately after manipulation, Endo-Fill and Endomethasone demonstrated the highest antimicrobial activity, with no statistically significant difference between them. Sealer 26 demonstrated the lowest antimicrobial activity. At all other times after manipulation, there were no statistically significant differences among all the sealers tested. In conclusion, none of the sealers totally inhibited the growth of the microorganisms. Furthermore, the antimicrobial activity of each sealer decreased with time and was dependent upon the microbial susceptibility to them.

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Phytochemical investigation and in vitro antimicrobial activity of Richardia scabra

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v11i2.24666 ◽

2016 ◽

Vol 11 (2) ◽

pp. 248 ◽

Cited By ~ 1

Author(s):

Kathirvel Poonkodi ◽

Subban Ravi

Keyword(s):

Antimicrobial Activity ◽

Diffusion Method ◽

Bacterial Strains ◽

Weed Species ◽

Antibacterial And Antifungal Activities ◽

Agar Plug ◽

Phytochemical Investigation ◽

Antibacterial And Antifungal ◽

Simple Sugar

The present study was aimed to evaluate the phytochemical screening and antimicrobial activity of the petroleum ether and methanol extracts from the mature leaves of Richardia scabra from India. Disc diffusion method was used to determine the zone inhibition of the tested samples for antibacterial and agar plug method was used to determine the antifungal activity, while the microtube-dilution technique was used to determine the minimum inhibitory concentration. Both extracts showed significant antibacterial and antifungal activities when tested against 10 bacterial and four fungal strains. The minimum inhibitory concentrations of the methanol extract of R. scabra ranged between 12.5–100 μg/mL for bacterial strains. Alkaloids, steroids, flavonoids, fatty acids, terpenoids and simple sugar were detected as phytoconstituents of extracts. To the best of our knowledge, this is the first report against antimicrobial activity of common weed species R. scabra found in India.

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