In vitro Antimicrobial Activity of Plants used in Cambodian Traditional Medicine

2007 ◽  
Vol 35 (05) ◽  
pp. 867-873 ◽  
Author(s):  
Aun Chea ◽  
Marie-Caroline Jonville ◽  
Sok-Siya Bun ◽  
Michèle Laget ◽  
Riad Elias ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Traditional Medicine ◽  
Bactericidal Effect ◽  
Gram Positive Bacteria ◽  
Antibacterial And Antifungal Activities ◽  
Methanolic Extracts ◽  
Disk Diffusion Assay ◽  
Antibacterial And Antifungal ◽  
Diffusion Assay

The purpose of the present study was to screen 27 plant species used in the traditional medicine of Cambodia for in vitro antibacterial and antifungal activities. Thirty-three methanolic extracts were tested against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Mycobacterium smegmatis and Candida albicans. Screened by disk diffusion assay, the extracts showed antimicrobial activity especially on Gram-positive bacteria. None of the crude methanolic extracts showed activity against P. aeruginosa. Twenty-five selected extracts were evaluated using a micro-dilution test. Harrisonia perforata (roots) and Hymenodictyon excelsum (bark) exhibited a bactericidal effect against S. aureus at a concentration of 500 μg/ml. Azadirachta indica (bark), Harrisonia perforata (roots and stem) and Shorea obtusa (roots) exhibited a bactericidal effect against M. smegmatis at 250 μg/ml.

scholarly journals Screening of antibacterial and antifungal activities of selected Macedonian wild mushrooms

2013 ◽  
pp. 333-340 ◽  
Author(s):  
Daniela Nikolovska-Nedelkoska ◽  
Natalija Atanasova-Pancevska ◽  
Haris Amedi ◽  
Dafina Veleska ◽  
Emilija Ivanova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Antimicrobial Properties ◽  
Inhibitory Effects ◽  
Antibacterial And Antifungal Activities ◽  
Microdilution Method ◽  
Methanolic Extracts ◽  
Antibacterial And Antifungal

Regarding the development of novel safe antimicrobials of natural origin, macrofungi became attractive for the researchers in the last decade. In this study, antimicrobial potential of methanolic extracts of six wild macromycetes (Boletus lupinus, Flammulina velutypes, Phellinus igniarius, Sarcodon imbricatus, Tricholoma aurantium, Xerocomus ichnusanus) was evaluated. In vitro antimicrobial activity was investigated by the microdilution method and minimum inhibitory concentration (MIC) was determined. Testing was conducted against eleven microorganisms, including six strains of bacteria and five species of fungi. Extracts showed selective antimicrobial properties while the activities depended both on the species of microorganism and on the type and concentration of extract. The evaluated extracts demonstrated antimicrobial activity, exhibiting more potent inhibitory effects on the growth of bacteria than on fungi. The highest antibacterial and antifungal activity was observed in methanolic extract of polypore fungus P. igniarius.

scholarly journals Phytochemical investigation and in vitro antimicrobial activity of Richardia scabra

2016 ◽  
Vol 11 (2) ◽  
pp. 248 ◽  
Author(s):  
Kathirvel Poonkodi ◽  
Subban Ravi
Keyword(s):  
Antimicrobial Activity ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Weed Species ◽  
Antibacterial And Antifungal Activities ◽  
Agar Plug ◽  
Phytochemical Investigation ◽  
Antibacterial And Antifungal ◽  
Simple Sugar

<p class="Abstract">The present study was aimed to evaluate the phytochemical screening and antimicrobial activity of the petroleum ether and methanol extracts from the mature leaves of <em>Richardia scabra</em> from India. Disc diffusion method was used to determine the zone inhibition of the tested samples for antibacterial and agar plug method was used to determine the antifungal activity, while the microtube-dilution technique was used to determine the minimum inhibitory concentration. Both extracts showed significant antibacterial and antifungal activities when tested against 10 bacterial and four fungal strains. The minimum inhibitory concentrations of the methanol extract of<em> R. scabra</em> ranged between 12.5–100 μg/mL for bacterial strains. Alkaloids, steroids, flavonoids, fatty acids, terpenoids and simple sugar were detected as phytoconstituents of extracts. To the best of our knowledge, this is the first report against antimicrobial activity of common weed species <em>R. scabra</em> found in India.</p><p> </p>

scholarly journals Antibacterial and Antifungal Activities of Rumex Confertus Willd

2021 ◽  
Vol 9 (12) ◽  
pp. 855-856
Author(s):  
G. D. Shermatova
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gram Positive ◽  
Antifungal Activities ◽  
Gram Positive Bacteria ◽  
Root Part ◽  
Fungal Activity ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal

Abstract: The leaves and roots of Rumex confertus Willd were tested in vitro for antibacterial and fungal activity in the fractions of gasoline, chloroform, ethyl acetate and butanol. As a result, it was found that the leaves of the Rumex confertus Willd plant, chloroform and ethyl acetate fractions of the root part have antibacterial activity against fungi and gram-positive bacteria. Keywords: Rumex confertus Willd, fractions, extracts, antibacterial, antifungal

scholarly journals Antimicrobial Activity ofLippiaSpecies from the Brazilian Semiarid Region Traditionally Used as Antiseptic and Anti-Infective Agents

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Cristiana da Purificação Pinto ◽  
Velize Dias Rodrigues ◽  
Fernanda da Purificação Pinto ◽  
Renata da Purificação Pinto ◽  
Ana Paula Trovatti Uetanabaro ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Fungal Infections ◽  
Active Species ◽  
Semiarid Region ◽  
Antimicrobial Drugs ◽  
Promising Alternative ◽  
Popular Medicine ◽  
Antibacterial And Antifungal Activities ◽  
Methanolic Extracts ◽  
Antibacterial And Antifungal

Lippia origanoidesKunth,Lippia alnifoliaSchauer, andLippia thymoidesMartius and Schauer are shrubs used in the traditional Brazilian medicine as antiseptics, as well as in the treatment of infectious diseases. This study was designed to investigate the antibacterial and antifungal activities of the methanolic extracts of these species, as new potential sources of antimicrobial drugs. The antimicrobial activity of methanolic extracts was investigated against resistant yeasts and bacteria by agar disk diffusion. Then, the MIC determination of the most active species and its fractions in hexane, dichloromethane, ethyl acetate, and water was performed. By the agar diffusion assay, all species were active against at least two microorganisms, giving evidence to support their use in the popular medicine.L. origanoidesleaves exhibited the widest antimicrobial action, inhibiting the growth of two Gram-positive bacteria and two yeasts; this activity was also confirmed by the MIC evaluation. The fractionation ofL. origanoidescrude extracts improved the activity in spectrum and intensity. The results obtained in this study indicate thatL. origanoidesmay be a promising alternative in the treatment of bacterial and fungal infections and in the seeking of new antimicrobial drugs.

scholarly journals Synthesis and Antimicrobial Activity of Pyrimidine Salts with Chloranilic and Picric Acids

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
C. Mallikarjunaswamy ◽  
D. G. Bhadregowda ◽  
L. Mallesha
Keyword(s):  
Antimicrobial Activity ◽  
The Other ◽  
Spectral Studies ◽  
H Nmr ◽  
Antibacterial And Antifungal Activities ◽  
Elemental Analyses ◽  
Ft Ir ◽  
Uv Visible ◽  
Antibacterial And Antifungal

Pyrimidine salts such as 2-methyl-5-nitro-phenyl-(4-pyridin-3-yl-pyrimidin-2-yl)-amine (1) and 4-methyl-3-(4-pyridin-3-yl-pyrimidin-2-yl-amino)-phenyl-amine (2) with chloranilic and picric acids were synthesized, and theirin vitroantibacterial and antifungal activities were evaluated. The synthesized compounds were characterized by elemental analyses, UV-visible, FT-IR, and1H NMR spectral studies. Compound2aexhibited good inhibition towards antimicrobial activity compared to the other compounds.

scholarly journals In vitro leaves and twigs antimicrobial properties of Psidium guajava L. (Myrtaceae)

2015 ◽  
Vol 61 (3) ◽  
pp. 93-104
Author(s):  
Basel Saleh ◽  
Lila Al-Halab ◽  
Ayman Al-Mariri
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Inhibitory Concentration ◽  
Activity Index ◽  
Antimicrobial Properties ◽  
Psidium Guajava ◽  
Hot Water ◽  
Antibacterial And Antifungal ◽  
Diffusion Assay

Summary The current study aimed to investigate antimicrobial activity of methanol, ethanol, acetone, ethyl acetate and hot water (leaves and twigs fractions) Psidium guajava L. crude extracts against three bacteria and two fungi pathogens. Antimicrobial activity expressed by disc-diffusion assay (zone of inhibitions - ZIs), activity index (AI) and minimum inhibitory concentration (MICs) that were measured as reported in many investigations. Based upon the estimated ZIs, AI and MICs values, hot water twigs <1 cm diameter extract was the most potent against all tested microorganisms. The MICs value ranged between 4 and 7.2 mg/ml for bacteria, while, it was between 14.5 and 37.3 mg/ml for fungi. Moreover, ethyl acetate had the lowest antimicrobial activity compared to the other tested solvents. From the results obtained herein, it could be concluded that P. guajava serve as antibacterial and antifungal agent.

scholarly journals Antimicrobial potential of 3-hydroxy-2-methylene-3-phenylpropionic acid derivatives

2011 ◽  
Vol 61 (4) ◽  
pp. 447-455 ◽  
Author(s):  
Soni Singh ◽  
Sujata Bhat
Keyword(s):  
Antimicrobial Activity ◽  
Low Cost ◽  
Aromatic Aldehydes ◽  
Dilution Method ◽  
Phenylpropionic Acid ◽  
Antimicrobial Potential ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal ◽  
Vinyl Derivatives

Antimicrobial potential of 3-hydroxy-2-methylene-3-phenylpropionic acid derivatives Twenty Baylis-Hillman adducts were synthesized from different aromatic aldehydes and activated vinyl derivatives. The adducts, which are differently substituted 3-hydroxy-2-methylene-3-phenylpropionic acid derivatives, were screened for their antimicrobial activity in vitro by the serial dilution method. Many of these molecules displayed potent antibacterial and antifungal activities. The ease of synthesis from low-cost starting materials along with potent antimicrobial activity of these molecules provide the lead for further improvement of activity and reflect the possibility of therapeutic use.

scholarly journals Antimicrobial activity of aqueous methanolic extracts of Algerian cultivars of sorghum (Sorghum bicolor (L.) Moench)

2020 ◽  
Vol 7 (1) ◽  
pp. 71-85
Author(s):  
Kadri Farida ◽  
Boudjeniba Messaoud ◽  
Wathelet Bernard ◽  
Akkal Salah
Keyword(s):  
Antimicrobial Activity ◽  
Sorghum Bicolor ◽  
Diffusion Method ◽  
Minimal Inhibition Concentration ◽  
Antibacterial And Antifungal Activities ◽  
Microdilution Method ◽  
Methanolic Extracts ◽  
Mrsa Strains ◽  
Antibacterial And Antifungal ◽  
Sorghum Bicolor L

AbstractCertain phenolics have been recognized to possessing antibacterial and antifungal activities and high levels of flavonoids and tannins have been reported in several varieties of Sorghum bicolor (L.) Moench. Antimicrobial activity and phenolics contents were investigated in five Algerian sorghum seeds. AS20 sorghum extract showed the highest levels of: total phenolics (3214.46±263.64 mg/100 g), flavonoids (32.03±1.64 mg/100 g) and tannins (615.35±6.10 mg/g) contents; however, comparable flavonoids content was recorded in I27 extract. FZ40 and AS12 flavonoids contents were comparable. Screening for antimicrobial activity, carried out by the disc’s diffusion method revealed an antimicrobial potential of sorghum crude extracts against Gram-positive and Gram-negative bacteria and candida albicans yeast. Minimal inhibition concentration determined by microdilution method varied between 0.2 and 2 mg/ml. the lowest value was recorded with F11 and FZ40 extract against Streptococcus pneumoniae and F11 against Escherichia coli. Bacillus subtilis, Staphylococcus aureus ATTCC6538 and MRSA strains showed sensitivity to all extracts. The results show these sorghums as a potential source of natural anti-streptococcal, anti-staphylococcal and anti-candida substances.

Antimicrobial Activity of Essential Oil from Pinus yunnanensis Franch.var tenuifolia Growing in China

2012 ◽  
Vol 430-432 ◽  
pp. 438-442 ◽  
Author(s):  
Yu Hong Tian ◽  
Zi Li ◽  
Shi Hua Wu ◽  
Qiong Huang
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Gas Chromatography Mass Spectrometry ◽  
Disc Diffusion ◽  
Bacterial Strains ◽  
Antibacterial And Antifungal Activities ◽  
Pinus Yunnanensis ◽  
Agar Dilution ◽  
Antibacterial And Antifungal

The in vitro antimicrobial activity of the essential oil from needles of Pinus yunnanensis Franch.var tenuifolia was investigated. The essential oil was obtained by hydro-distillation and analyzed by gas chromatography-mass spectrometry (GC-MS) method. The major compound of the essential oil was α-pinene (22.542%), followed by β-caryophyllene (16.636%), 1,2,4a,5,8,8a-hexahydro-4,7-dimethyl-1-(1-methylethyl)-naphthalene (5.085%), β-cubebene (4.779%), elixene (4.442%), α-cadinol (3.528%),α-caryophyllene (3.459%) and limonene (3.428%). The antimicrobial activity of oil was evaluated by using disc diffusion and agar dilution methods, respectively. The results indicated that the essential oil held moderate antimicrobial activity against tested microorganisms. The essential oil did not differ remarkably in its antibacterial and antifungal activities. The minimum inhibitory concentrations (MICs) were 40 ml/l for bacterial strains, and 80 ml/l for fungal strains.

scholarly journals Synthesis andIn VitroBiological Evaluation of Aminonaphthoquinones and Benzo[b]phenazine-6,11-dione Derivatives as Potential Antibacterial and Antifungal Compounds

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Amaç Fatih Tuyun ◽  
Nilüfer Bayrak ◽  
Hatice Yıldırım ◽  
Nihal Onul ◽  
Emel Mataraci Kara ◽  
...  
Keyword(s):  
Spectral Properties ◽  
Antifungal Compounds ◽  
New Members ◽  
Gram Positive Bacteria ◽  
H Nmr ◽  
Antibacterial And Antifungal Activities ◽  
Aryl Amines ◽  
Antibacterial And Antifungal ◽  
C Nmr

A series of 2-arylamino-3-chloro-1,4-naphthoquinone derivatives (3a–h) by the reaction of 2,3-dichloro-1,4-naphthoquinone with aryl amines (2a–h) and benzo[b]phenazine-6,11-dione derivatives (4a–c) by the treatment of 2-arylamino-3-chloro-1,4-naphthoquinone derivatives (3a–h) with sodium azide were synthesized and tested for theirin vitroantibacterial and antifungal activities. The results suggest that compounds3dand3ghad potent antifungal activity againstCandida albicans(MIC = 78.12 μg/mL). All synthesized compounds (3a–h,4a–c) possessed activity againstE. faecaliswith MIC values of between 312.5 and 1250 μg/mL. Benzo[b]phenazine-6,11-dione derivatives (4a–c) were mostly active against Gram-positive bacteria. The structures of the new members of the series were established on the basis of their spectral properties (IR,1H NMR,13C NMR, and mass spectrometry).

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