Analgesic, anti-inflammatory, and anti-oxidant activities of Phlogacanthus thyrsiflorus leaves

2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Biplab Kumar Das ◽  
Md. Mamun Al-Amin ◽  
Nusrat Nabila Chowdhury ◽  
Md. Fakhar Uddin Majumder ◽  
Mohammad Nasir Uddin ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Dose Range ◽  
Radical Scavenging ◽  
Hot Plate Test ◽  
Phytochemical Study ◽  
Percolation Method ◽  
Anti Oxidant ◽  
Anti Inflammatory

AbstractOur present study was carried out to explore the potential role of the methanol extract from the leaves ofMethanol extracts of PT leaves were prepared using 500 g powder in 1.8 L methanol by percolation method, followed by evaporation in a rotary evaporator under controlled temperature and pressure. The crude methanol extract was dried by freeze drier and preserved at 4 °C.Oral administration of PT significantly (p<0.05) increased the reaction time at 55.73% (250 mg/kg) and 72.81% (500 mg/kg) inhibition (p<0.05) in the hot plate test at 3 h. PT significantly (p<0.05) inhibited 42.17% (250 mg/kg) and 56.63% (500 mg/kg) acetic acid-induced writhing. PT leaves (250 and 500 mg/kg) also significantly (p<0.05) inhibited paw edema 6 h after carrageenan injection. Furthermore, this plant showed significant (p<0.05) free radical-scavenging activity at a dose range of 25–800 μg/mL.Based on the findings, we can conclude that PT leaf possesses analgesic, anti-inflammatory, and anti-oxidant activities. Preliminary phytochemical study of PT leaves revealed the presence of flavonoids, tannins and triterpens in methanol extract which could be correlated with its observed biological activities.

Evaluation of Anti-oxidant and Anti-pyretic activity of fruits of Garcinia pedunculata

2021 ◽  
pp. 2635-2638
Author(s):  
Mrinmoy Basak ◽  
Moksood Ahmed Laskar ◽  
Priyanka Goswami
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Synthetic Drugs ◽  
Long Time ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Most of the synthetic drugs used at present as analgesic and anti-inflammatory agents cause many side effects and toxic effects. Many medicines of plant origin with analgesic and anti-inflammatory activity have been used since long time without adverse effects. The purpose of the study was to explore the anti-pyretic and anti-oxidant activity of fruits of Garcinia pedunculata. The fruit of G. pedunculata was collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract shows the presence of carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 4 hours, 8 hours and 12 hours after giving the extract dose of 100mg/kg and 200mg/kg body weight, and it was found to produce a significant reduce in fever. DPPH radical scavenging activity (In-vitro anti-oxidant activity of G. pedunculata) of methanolic extract shows 38.31 as IC50 (µg/ml) whereas Ascorbic acid as standard in the same concentration shows 34.81 IC50 (µg/ml). The obtained result justified the traditional use of G. pedunculata as anti-pyretic and antioxidant purpose.

scholarly journals Evaluation of Anti-Oxidant and Anti-Pyretic Activity of Fruit of Garcenia Padunculata

2020 ◽  
Vol 8 (3) ◽  
pp. 77-80
Author(s):  
Basak Mrinmoy ◽  
Laskar Ahmed Moksood ◽  
Goswami Priyanka
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Synthetic Drugs ◽  
Long Time ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Most of the synthetic drugs used at present as analgesic and anti-inflammatory agents cause many side effects and toxic effects. Many medicines of plant origin with analgesic and anti-inflammatory activity have been used since long time without adverse effects. The purpose of the study was to explore the anti-pyretic and anti-oxidant activity of fruits of Garcinia pedunculata. The fruit of G. pedunculatawas collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract shows the presence of carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 4 hours, 8 hours and 12 hours after giving the extract dose of 100mg/kg and200mg/kg body weight, and it was found to produce a significant reduce in fever. DPPH radical scavenging activity (In-vitro anti-oxidant activity of G. pedunculata) of methanolic extract shows 38.31 as IC50 (µg/ml) whereas Ascorbic acid as standard in the same concentration shows34.81 IC50 (µg/ml). The obtained result justified the traditional use of G. pedunculata as anti-pyretic and antioxidant purpose.    

scholarly journals FORMULATION, CHARACTERIZATION AND EVALUATION OF ANTI-INFLAMMATORY AND ANTI-ANGIOGENIC ACTIVITIES OF MEMECYLAENE NANOEMULSION

2018 ◽  
Vol 8 (5-s) ◽  
pp. 126-131
Author(s):  
ND Rekha ◽  
Dattatri K. Nagesha ◽  
PH Rajasree ◽  
N Shruthi
Keyword(s):  
Zeta Potential ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vivo Model ◽  
Particle Size Range ◽  
Nitric Oxide Radical ◽  
Anti Oxidant ◽  
Anti Inflammatory

The present study was undertaken to formulate and evaluate the anti-inflammatory, anti-oxidant and anti-angiogenic activities of nanoemulsion of Memecylaene.  Memecylaene was isolated from the leaves of Memecylon malabaricum by using various chromatographic methods. An oil-in-water (O/W) nanoemulsion of Memecylaene was formulated by sonication method using sunflower oil (oil phase), Tween 80 (Surfactant) and Ethanol (co-surfactant). The prepared nanoemulsion was characterized for its droplet size, poly dispersity index and zeta potential. Stability studies were performed and the nanoemulsions were subjected to different biological activities. The formulated nanoemulsion had a particle size range of 52.02 nm to 59.47 nm and zeta potential of -1.27 mV. The enhanced activity of Memecylaene, encapsulated in O/W emulsions is evidenced by the inhibition of phospholipase (PLA2) enzyme and H+, K+ -ATPase and thus showing anti-inflammatory and anti-secretagogues effects. The in vitro anti-oxidant activity was evaluated by DPPH radical and Nitric oxide radical scavenging activity. Further, the inhibition of the growth of neo vessels formation in the in-vivo model system of chick chorioallantoic membrane (CAM) assay, which is angiogenesis dependent, was also observed. The above findings would help in understanding the putative potential of Memecylaene-loaded nanoemulsion as a therapeutic agent. Keywords: Anti-angiogenesis, Anti-oxidant, Gastric (H+ K+), Memecylaene, Nanoemulsion, Phospholipase A2 (PLA2).

α-Glucosidase Inhibition, Antioxidant and Docking Studies of Hydroxycoumarins and their Mono and Bis O-alkylated/acetylated Analogs

2018 ◽  
Vol 15 (2) ◽  
pp. 127-135 ◽  
Author(s):  
Parvesh Singh ◽  
Nomandla Ngcoya ◽  
Ramgopal Mopuri ◽  
Nagaraju Kerru ◽  
Neha Manhas ◽  
...  
Keyword(s):  
In Silico ◽  
Biological Activities ◽  
Wide Spectrum ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Catalytic Site ◽  
Docking Simulations ◽  
In Silico Docking ◽  
Anti Oxidant

Background: Diabetes Mellitus (DM) is a complex metabolic disease illustrated by abnormally high levels of plasma glucose or hyperglycaemia. Accordingly, several α-glucosidase inhibitors have been developed for the treatment of diabetes and other degenerative disorders. While, a coumarin ring has the privilege to represent numerous natural and synthetic compounds with a wide spectrum of biological activities e.g. anti-cancer, anti-HIV, anti-viral, anti-malarial, anti-microbial, anti-convulsant, anti-hypertensive properties. Besides this, coumarins have also shown potential to inhibit α-glucosidase leading to a generation of new promising antidiabetic agents. However, the testing of O-substituted coumarins for α-glucosidase inhibition has evaded the attention of medicinal chemists. Methods: For O-alkylation/acetylation reactions, the hydroxyl coumarins (A-B) initially activated by K2CO3 in dry DMF were reacted with variedly substituted haloalkanes at room temperature under nitrogen. The synthesized compounds were tested for their α-glucosidase (from Saccharomyces cerevisiae) inhibitory activity and anti-oxidant activity using DPPH radical scavenging activity. In silico docking simulations were conducted using CDocker module in DS (Accelrys) to explore the binding modes of the representative compounds in the catalytic site of α-glucosidase. Results: All the coumarin analogues (A1, B1, A2-A10, B2-B8) including their precursors (A-B) were evaluated for their in vitro α-glucosidase inhibition using acarbose as a standard inhibitor. All the mono O-alkylated coumarins (except A1) showed significant (p <0.05) α-glucosidase inhibition relative to the hydroxyl coumarin (A) with IC50 values ranging between 11.084±0.117 to 145.24± 29.22 µg/mL. Compound 7-(benzyloxy)-4, 5-dimethyl-2H-chromen-2-one (A9) bearing a benzyl group (Ph-CH2-) at position 7 showed a remarkable (p <0.05) increase in the activity (IC50 = 11.084±0.117 µg/mL), almost four-fold more than acarbose (IC50 = 40.578±5.999 µg/mL). The introduction of –NO2 group dramatically improved the anti-oxidant activity of coumarin, while the O-alkylation/acetylation decreased the activity. Conclusion: The present study describes the synthesis of functionalized coumarins and their evaluation for α-glucosidase inhibition and antioxidant activity under in vitro conditions. Based on IC50 data, the mono O-alkylated coumarins were observed to be stronger inhibitors of α-glucosidase with respect to their bis O-alkylated analogues. Coumarin (A9) bearing O-benzyloxy group displayed the strongest α-glucosidase inhibition, even higher than the standard inhibitor acarbose. The coumarin (A10) bearing –NO2 group showed the highest anti-oxidant activity amongst the synthesized compounds, almost comparable to the ascorbic acid. Finally, in silico docking simulations revealed the role of hydrogen bonding and hydrophobic forces in locking the compounds in catalytic site of α-glucosidase.

scholarly journals A New Macrolactone, Racemolide Along With Seven Known Compounds With Biological Activities From Mangrove Plant,Lumnitzera racemosa

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1986125
Author(s):  
Ahmed Gomaa Gomaa Darwish ◽  
Mamdouh Nabil Samy ◽  
Sachiko Sugimoto ◽  
Hideaki Otsuka ◽  
Katsuyoshi Matsunami
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Dpph Radical ◽  
Mangrove Plant ◽  
Anti Oxidant ◽  
Dpph Radical Scavenging ◽  
Lumnitzera Racemosa

One new macrolide, racemolide (1), was isolated from an MeOH extract of the leaves of Lumnitzera racemosa with 7 known compounds (2-8) by various chromatographic techniques via silica gel, octadecylsilyl column chromatography and high-performance liquid chromatography. The structure of the new compound was determined through a combination of spectroscopic and chemical analyses. The isolated compounds were evaluated for their antileishmanial, hepatoprotective, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities. Compound 1 showed potent antileishmanial activity at inhibition % value of 67.6 ± 1.24% compared with the clinical drug miltefosine (93.3 ± 4.39%). Compounds 1 and 5 possessed moderate hepatoprotective activity against acetaminophen (APAP)-induced hepatotoxicity using human HepG2 cells at protection % value of 21.70 ± 1.97% and 27.5 ± 1.41%, respectively, compared with the standard glycyrrhizin (69.50 ± 3.53%). Compounds 4, 6, 7, and 8 had high DPPH radical scavenging activity (91.10 ± 4.25, 91.53 ± 0.05, 91.91 ± 0.09, and 86.48 ± 2.87%, respectively), while compounds 3 and 5 showed moderate (45.38 ± 3.62% and 62.20 ± 1.47%, respectively), comparable with the standard trolox (89.21 ± 5.88%). These results suggest that the new compound, racemolide (1), and known compounds (3-8) are valuable as promising antileishmanial, hepatoprotective, and anti-oxidant agents.

scholarly journals Phytochemical profiling, and anti-oxidant, anti-bacterial, and anti-inflammatory properties of Viburnum coriaceum Blume

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Vijayakumar Vijaytha ◽  
R. V. Anupama ◽  
M. Haridas
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Zone Of Inhibition ◽  
Agar Gel ◽  
Anti Oxidant ◽  
Medicinal Properties ◽  
Phytochemical Profile ◽  
Anti Inflammatory ◽  
Drug Leads ◽  
Diffusion Assay

Abstract Background Viburnums comprise a taxonomic group of plants distributed all over the world and were reported to have many biological activities. Viburnum coriaceum Blume is one of the least explored members of the group. The present study was aimed to explore the phytochemical profile of the plant, Viburnum coriaceum Blume with special emphasis to its anti-oxidant, anti-bacterial, and anti-inflammatory activities. Results Extracts of all parts of plants were found to possess a spectrum of chemicals in considerable amount, including triterpenoids and glycosides. Anti-oxidant property was found in extracts made of any part of the plant. Methanol extract of root expressed a prominent zone of inhibition in agar gel well-diffusion assay involving many microorganisms. Inhibition of enzymes, LOX, and trypsin showed by the hexane extract of root suggested a prominent anti-inflammatory potential of the plant under investigation. Conclusion Phytochemical profiling and the other assays using the plant extracts provide us with a plant having many valuable medicinal properties. Viburnum coriaceum Blume could be noted as a promising material for drug leads.

scholarly journals Phytochemical composition and biological investigation of Trichilia emetica Vahl. seed extracts

2020 ◽  
Vol 9 (2) ◽  
pp. 1111-1116
Keyword(s):  
Antifungal Activity ◽  
Ethyl Acetate ◽  
Fungal Pathogens ◽  
Methanol Extract ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Phytochemical Analysis ◽  
Biological Investigation ◽  
Seed Extracts

The current study investigated the phytochemical analysis, chemical composition, antioxidant, cytotoxic and antifungal activity of Trichilia emetica seed extracts. Preliminary phytochemical screening and quantification were realized by GC-MS. Consecutive extractions (methanol, ethanol, ethyl acetate, hexane, chloroform and distilled water) were carried out and phytochemical tests performed to represent the variety of phytochemicals. Seed extracts variably displayed alkaloids, cardiac glycosides, phenols, sterols, terpenoids and flavonoids but there was no indication of saponins and tannins in all extracts. The biological investigation of the methanolic extract showed significant biological activities. The results obtained revealed that methanol extract has presented significant IC50 =5.94 µg/mL radical scavenging activity. Minimum inhibitory concentration (MIC) of hexane and chloroform fractions displayed antifungal activity (40.95-100 and 76.27-100.11, respectively) against all three fungal pathogens, whilst the methanol extract inhibited C. krusei and C. parapsilosis and the ethyl acetate inhibited C. parapsilosis only. The results obtained revealed the medicinal importance of the plant and methanol extract of T. emetica would aid researchers to exploit as potential antifungal and antioxidant agent.

scholarly journals A Comparative Study on Antioxidation and Antibacterial Activities Triphala Herb Extracts from Chae Son, Lampang, Thailand

2020 ◽  
pp. 1-14
Author(s):  
Wilart Pompimon ◽  
Wipanoot Baison ◽  
Angkhana Chuajedton ◽  
Phansuang Udomputtimekakul ◽  
Punchavee Sombutsiri ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Antioxidant Power ◽  
Methanolic Extracts

Introduction: Triphala, a herbal medicine which is a product from Chae Son, Lampang, Thailand, is an unequal-proportional mixture of fruits of three herbs, Phyllanthus emblica, Terminalia chebula, and Terminalia bellirica (2:1:1). Materials and Methods: The contemporary study concentrated on extracts detection and comparative analysis between various annual year (A.D.2017/2018) activities in hexane, ethyl acetate, and methanolic extracts of Triphala. The determination of antioxidant was using by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP). The broth dilution and agar well diffusion assay were determined antibacterial. Results: Results exposed the valuable biological activity extracts from ethyl acetate and methanol solvents of T. chebula and T. bellirica, which strength is accountable for biological activities. Extracts displayed acceptable radical-scavenging activity analogous with ascorbic acid. The methanol extract of T. bellirica (0.0013 mg/mL), ethyl acetate extract of P. emblica (0.0009 mg/mL), in A.D. 2017 and the ethyl acetate extract of P. emblica (0.0090 mg/mL), the methanol extract of Triphala (0.0176 mg/mL) in 2018, were exhibited higher antioxidant activity. Also, extracts indicated hopeful antibacterial potential against tested strain comparable to chloramphenicol. Conclusion: The results in A.D. 2017 showed that inhibition zone of P. emblica in ethyl acetate extract; S. aureas (8.67 mm), E. aerogenes (8.67 mm), E. coli (EPEC) (7.67 mm), P. mirabilis (7.73 mm) , S. typhimurium (8.33 mm), S. flexneri (8.33 mm) and V. cholera (7.33 mm) together with P. emblica methanol extract; S. aureas (6.67 mm), E. aerogenes (7.76 mm.)  and V. cholera (4.0 mm). Therefore, it could be concluded that Triphala may be a talented candidate in pharmaceutical and future medicine.

Terpenoids and phytosteroids isolated from Commelina benghalensis Linn. with antioxidant activity

2019 ◽  
Vol 31 (1) ◽  
Author(s):  
Amina Khatun ◽  
Mahmudur Rahman ◽  
Mohammad Sharifur Rahman ◽  
Md. Khalid Hossain ◽  
Mohammad Abdur Rashid
Keyword(s):  
Spectroscopic Analysis ◽  
Methanol Extract ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Property ◽  
Medicinal Uses ◽  
Commelina Benghalensis ◽  
Aerial Parts ◽  
Dpph Radical Scavenging

AbstractBackgroundCommelina benghalensis Linn. (Family: Commelinaceae) is a common weed available in Bangladesh with several uses in traditional medicine. However, the chemical profile of this medicinal plant is scarce in relation to its medicinal uses. The aerial parts of this plant have been investigated for the isolation of secondary metabolites and evaluation of the biological activities.MethodsMajor phytochemical groups were analyzed using chromogenic reagents, whereas n-hexane soluble fractionates of the methanol extract were subjected to 1H nuclear magnetic resonance (NMR) spectroscopic analysis. The antioxidant property of the obtained compounds was evaluated using 1,1-diphenyl-2-picryl-hydrazyl (DPPH).ResultsDammara-12-en-3-one (CB-1), stigmasterol (CB-2) and 3 (2,3,4,5,6-pentahydroxy)-cinnamoyl dammara-12-ene (CB-3) were isolated from the n-hexane fractionate of methanol extract of C. benghalensis. In the study of DPPH radical scavenging activity, IC50 values were predicted to be 790.18, 4186.94 and 2001.16 μg/mL for CB-1, CB-2 and CB-3, respectively, whereas standard ascorbic acid showed IC50 at 1.26 μg/mL.ConclusionsTwo new dammarane-type triterpene (CB-1 and CB-3) and one phytosterol (CB-2) were identified in C. benghalensis with mild antioxidant property.

scholarly journals Phenolic profile and biological activity of table grapes (Vitis vinifera L.)

2019 ◽  
Vol 15 ◽  
pp. 04005
Author(s):  
C. Di Lorenzo ◽  
F. Colombo ◽  
E. Sangiovanni ◽  
S. Biella ◽  
L. Regazzoni ◽  
...  
Keyword(s):  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inflammatory Activity ◽  
Array Detector ◽  
Vitis Vinifera L ◽  
Positive Effects ◽  
Table Grapes ◽  
Anti Inflammatory ◽  
Grape Varieties

Table grapes are largely produced and consumed in the Mediterranean area. Furthermore, in the last years, the commercial interest in table grapes and other non-alcoholic grape products has notably increased worldwide. In addition to the nutritional aspects, polyphenol compounds in fresh grapes could exert positive effects on human health. The aim of this study was the characterization of the phenolic pattern of sixteen grape varieties and the evaluation of the associated antioxidant capacity and anti-inflammatory activity at gastric level. The methods used were: 1) Folin-Cocalteau's assay for the quantification of total polyphenol content; 2) High-Performance Liquid Chromatography (HPLC) coupled with Diode-Array Detector (DAD) to perform the quantitative analysis of grapes phenolic fraction 3) DPPH (1,1,-diphenil-2-picrylhydrazyl) spectrophotometric assay for the assessment of radical scavenging activity; 4) assessment of IL-8 release from human gastric epithelial cells to evaluate the anti-inflammatory activity of grape extracts. Some grapes, including seedless varieties, showed that the phenolic pattern was highly correlated with the biological activities, and, in particular, with peel and seeds portion. These results suggest that selected grape varieties could represent, also for consumers who do not drink wine, a source of healthy compounds potentially able to counteract oxidative stress and gastric inflammation.

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