scholarly journals Evaluation of Anti-Oxidant and Anti-Pyretic Activity of Fruit of Garcenia Padunculata

2020 ◽  
Vol 8 (3) ◽  
pp. 77-80
Author(s):  
Basak Mrinmoy ◽  
Laskar Ahmed Moksood ◽  
Goswami Priyanka
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Synthetic Drugs ◽  
Long Time ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Most of the synthetic drugs used at present as analgesic and anti-inflammatory agents cause many side effects and toxic effects. Many medicines of plant origin with analgesic and anti-inflammatory activity have been used since long time without adverse effects. The purpose of the study was to explore the anti-pyretic and anti-oxidant activity of fruits of Garcinia pedunculata. The fruit of G. pedunculatawas collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract shows the presence of carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 4 hours, 8 hours and 12 hours after giving the extract dose of 100mg/kg and200mg/kg body weight, and it was found to produce a significant reduce in fever. DPPH radical scavenging activity (In-vitro anti-oxidant activity of G. pedunculata) of methanolic extract shows 38.31 as IC50 (µg/ml) whereas Ascorbic acid as standard in the same concentration shows34.81 IC50 (µg/ml). The obtained result justified the traditional use of G. pedunculata as anti-pyretic and antioxidant purpose.    

Evaluation of Anti-oxidant and Anti-pyretic activity of fruits of Garcinia pedunculata

2021 ◽  
pp. 2635-2638
Author(s):  
Mrinmoy Basak ◽  
Moksood Ahmed Laskar ◽  
Priyanka Goswami
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Synthetic Drugs ◽  
Long Time ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Most of the synthetic drugs used at present as analgesic and anti-inflammatory agents cause many side effects and toxic effects. Many medicines of plant origin with analgesic and anti-inflammatory activity have been used since long time without adverse effects. The purpose of the study was to explore the anti-pyretic and anti-oxidant activity of fruits of Garcinia pedunculata. The fruit of G. pedunculata was collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract shows the presence of carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 4 hours, 8 hours and 12 hours after giving the extract dose of 100mg/kg and 200mg/kg body weight, and it was found to produce a significant reduce in fever. DPPH radical scavenging activity (In-vitro anti-oxidant activity of G. pedunculata) of methanolic extract shows 38.31 as IC50 (µg/ml) whereas Ascorbic acid as standard in the same concentration shows 34.81 IC50 (µg/ml). The obtained result justified the traditional use of G. pedunculata as anti-pyretic and antioxidant purpose.

scholarly journals Evaluation of Anti-oxidant and Anti-pyretic Activity of Leaf of Dendrobium chrysanthum

2021 ◽  
pp. 389-394
Author(s):  
Mrinmoy Basak ◽  
Pal Gogoi ◽  
Sajidul Hoque Ansari ◽  
Biplab Kumar Dey ◽  
Saikat Sen ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging ◽  
Traditional Use ◽  
Rural People ◽  
The North ◽  
North Eastern ◽  
Anti Oxidant ◽  
Dendrobium Chrysanthum ◽  
Dpph Radical Scavenging

This investigation is based on a very remarkable and interesting orchid of the genus Dendrobium widely available in Meghalaya (India) and it is the world second largest orchid genus. The purpose of the study was to evaluate the anti-oxidant and anti-pyretic activity of leaf of Dendrobium chrysanthum. As the plants believed traditionally to have some anti-inflammatory activity used by the rural people of Meghalaya, use of medicinal plants and plants extract  for the health procurement has always remain a common choice for the North- eastern people of India since ancient time. The leaf of Dendrobium c. was collected from Shillong, Meghalaya and extraction is done by using methanol as a solvent. The methanol extract found to have the constituents such as carbohydrate, glycoside, alkaloids, phenol and flavonoids. Acute anti-pyretic activity for the extract was investigated in pyrexia rat. Temperature level was determined after 1hours, 2 hours, 4 hours and 6 hours after giving the extract dose of 100mg/kg and 200mg/kg body weight, and it was found to show potent anti-pyretic activity by reducing the temperature in rat. In-vitro anti-oxidant activity was studied by DPPH radical scavenging method of methanolic extract which shows 36.20 as IC50 (µg/ml) whereas the standard Ascorbic acid in the same concentration shows 32.81 IC50 (µg/ml). The obtained result justified the traditional use of Dendrobium c. as anti-pyretic and antioxidant purpose.

scholarly journals In Vitro Toxicity, Antioxidant, Anti-Inflammatory, and Antidiabetic Potential of Sphaerostephanos unitus (L.) Holttum

Antibiotics ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 333 ◽  
Author(s):  
Marimuthu Alias Antonisamy Johnson ◽  
C. Xavier Madona ◽  
Ray S. Almeida ◽  
Natália Martins ◽  
Henrique D. M. Coutinho
Keyword(s):  
Petroleum Ether ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Toxicity ◽  
Dependent Manner ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Pteridophytes have been widely used in several systems of medicine. Several reports have increasingly assessed their bioactive effects, but for Sphaerostephanos unitus (L.) Holttum, only its antibacterial potential has been assessed. In this sense, the present study was carried out to reveal the phytochemical profile and to determine the toxicity, antioxidant, antidiabetic, and anti-inflammatory potential of S. unitus. Brine shrimp lethality, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, phosphomolybdenum assay, superoxide radical scavenging activity, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assay (ABTS), and in vitro α-amylase inhibitory and membrane stabilization assays were applied. S. unitus extract toxicity showed variable mortality percentages, with LC50 values ranging from 4 to 30 mg/mL. DPPH radical scavenging effects of S. unitus extracts were as follows: methanol > acetone > petroleum ether > chloroform. S. unitus acetone extract displayed the strongest phosphomolybdenum reduction (10 ± 2 mg Ascorbic Acid Equivalent/g). The studied extracts also revealed efficient, superoxide scavenging effects in a dose-dependent manner. In S. unitus, the highest ABTS radical scavenging rate was observed in the chloroform extract (3000 ± 40 µmol/g). The S. unitus anti-inflammatory effect was as follows: petroleum ether > chloroform > methanol > acetone. In S. unitus extract, the highest percentage of α-amylase activity (80%) was observed for the petroleum ether extract (25 µg/mL). Faced with these findings, further studies should be performed to isolate and identify the S. unitus compounds responsible for their antioxidant, antidiabetic and anti-inflammatory effects.

scholarly journals FORMULATION, CHARACTERIZATION AND EVALUATION OF ANTI-INFLAMMATORY AND ANTI-ANGIOGENIC ACTIVITIES OF MEMECYLAENE NANOEMULSION

2018 ◽  
Vol 8 (5-s) ◽  
pp. 126-131
Author(s):  
ND Rekha ◽  
Dattatri K. Nagesha ◽  
PH Rajasree ◽  
N Shruthi
Keyword(s):  
Zeta Potential ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vivo Model ◽  
Particle Size Range ◽  
Nitric Oxide Radical ◽  
Anti Oxidant ◽  
Anti Inflammatory

The present study was undertaken to formulate and evaluate the anti-inflammatory, anti-oxidant and anti-angiogenic activities of nanoemulsion of Memecylaene.  Memecylaene was isolated from the leaves of Memecylon malabaricum by using various chromatographic methods. An oil-in-water (O/W) nanoemulsion of Memecylaene was formulated by sonication method using sunflower oil (oil phase), Tween 80 (Surfactant) and Ethanol (co-surfactant). The prepared nanoemulsion was characterized for its droplet size, poly dispersity index and zeta potential. Stability studies were performed and the nanoemulsions were subjected to different biological activities. The formulated nanoemulsion had a particle size range of 52.02 nm to 59.47 nm and zeta potential of -1.27 mV. The enhanced activity of Memecylaene, encapsulated in O/W emulsions is evidenced by the inhibition of phospholipase (PLA2) enzyme and H+, K+ -ATPase and thus showing anti-inflammatory and anti-secretagogues effects. The in vitro anti-oxidant activity was evaluated by DPPH radical and Nitric oxide radical scavenging activity. Further, the inhibition of the growth of neo vessels formation in the in-vivo model system of chick chorioallantoic membrane (CAM) assay, which is angiogenesis dependent, was also observed. The above findings would help in understanding the putative potential of Memecylaene-loaded nanoemulsion as a therapeutic agent. Keywords: Anti-angiogenesis, Anti-oxidant, Gastric (H+ K+), Memecylaene, Nanoemulsion, Phospholipase A2 (PLA2).

In vitro Anti-oxidant and Anti-inflammatory Activity of the Aqueous and Ethanol Leaf Extracts of Clerodendrum phlomidis Linn. F.

2021 ◽  
pp. 5217-5221
Author(s):  
Krishnaveni R ◽  
Rajan S.
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Scavenging Activity ◽  
Rbc Membrane ◽  
Clerodendrum Phlomidis ◽  
Anti Oxidant ◽  
Anti Inflammatory

Clerodendrum phlomid is was a common shrub of arid plains, low hills, and tropical deserts. It belongs to the family Verbenaceae. Many species of this genus have been documented in traditional system of medicine in India. The roots of the plant have been extensively studied in Ayurveda but in folk and traditional medicine, it is the leaf that finds more application in alleviating many diseases. Oxidation byproducts of metabolism was responsible for many pathophysiological conditions. Anti-oxidants are molecules that quench the free radicals. The present study aims at evaluating the in-vitro anti-oxidant and anti-inflammatory activity of the aqueous and ethanol leaf extract of Clerodendrum phlomidis. The anti-oxidant activity was evaluated by three in vitro models, namely the DPPH radical scavenging activity, hydroxyl radical scavenging activity and NO radical scavenging activity. The anti-inflammatory activity was evaluated by the RBC membrane Inhibition method. The aqueous extract was found to have better anti- oxidant activity than the ethanol extract in DPPH and NO radical scavenging activity. The RBC membrane inhibition method clearly states that the aqueous leaf extract of Clerodendrum phlomidis was found to have a comparatively an effective anti-inflammatory activity than ethanol extract.

Analgesic, anti-inflammatory, and anti-oxidant activities of Phlogacanthus thyrsiflorus leaves

2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Biplab Kumar Das ◽  
Md. Mamun Al-Amin ◽  
Nusrat Nabila Chowdhury ◽  
Md. Fakhar Uddin Majumder ◽  
Mohammad Nasir Uddin ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Dose Range ◽  
Radical Scavenging ◽  
Hot Plate Test ◽  
Phytochemical Study ◽  
Percolation Method ◽  
Anti Oxidant ◽  
Anti Inflammatory

AbstractOur present study was carried out to explore the potential role of the methanol extract from the leaves ofMethanol extracts of PT leaves were prepared using 500 g powder in 1.8 L methanol by percolation method, followed by evaporation in a rotary evaporator under controlled temperature and pressure. The crude methanol extract was dried by freeze drier and preserved at 4 °C.Oral administration of PT significantly (p<0.05) increased the reaction time at 55.73% (250 mg/kg) and 72.81% (500 mg/kg) inhibition (p<0.05) in the hot plate test at 3 h. PT significantly (p<0.05) inhibited 42.17% (250 mg/kg) and 56.63% (500 mg/kg) acetic acid-induced writhing. PT leaves (250 and 500 mg/kg) also significantly (p<0.05) inhibited paw edema 6 h after carrageenan injection. Furthermore, this plant showed significant (p<0.05) free radical-scavenging activity at a dose range of 25–800 μg/mL.Based on the findings, we can conclude that PT leaf possesses analgesic, anti-inflammatory, and anti-oxidant activities. Preliminary phytochemical study of PT leaves revealed the presence of flavonoids, tannins and triterpens in methanol extract which could be correlated with its observed biological activities.

scholarly journals Effects of Namya Kanom Jeen powder extracts on anti-oxidative and anti-inflammatory properties

2018 ◽  
Vol 8 (8) ◽  
pp. 379 ◽  
Author(s):  
Preeya Dat-arun ◽  
Rattana Leelawattana ◽  
Pavinee Chinachoti
Keyword(s):  
Functional Properties ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Water Extract ◽  
No Production ◽  
Scavenging Activity ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Anti Inflammation

Background: Spices and herbs are known to have anti-oxidant and anti-inflammatory properties. We studied their properties of Namya Kanom Jeen (NKJ), a spicy soup (Southern Thailand recipe) with water and ethanol (50% and 95%) extracts.Methods: This study aimed to assess functional properties of NKJ powder extract using in vitro model. These functional properties were anti-oxidant, α-amylase inhibition, and anti-inflammatory properties. Anti-oxidant activities were determined using free radical scavenging activity (DPPH) and Ferric Reducing Anti-oxidant Power (FRAP). Anti-inflammation effect was studied by measuring nitric oxide (NO) production inhibition on RAW264.7 macrophage cells after being exposed to lipopolysaccharide (LPS). Results: Water extract of NKJ powder demonstrated the highest activity in anti-inflammatory and anti-oxidant property by DPPH radical scavenging activity when compared with ethanol extract. Conclusion: NKJ powder extracts may ameliorate the oxidation and inflammation conditions which would be the combined effect of several bioactive compounds from many of the ingredients within these extracts.Keywords: Anti-oxidant; Anti-inflammation; Phenolic compound; Namya Kanom Jeen powder

scholarly journals Antioxidant, anti-inflammatory, analgesic and anti-proliferating activities of Grewia heterotricha Mast.

Biomedicine ◽  
2020 ◽  
Vol 40 (3) ◽  
pp. 272-277
Author(s):  
B. Usha ◽  
Karanth Jyothsna ◽  
Joshi Chandrashekhar G.
Keyword(s):  
Aqueous Extract ◽  
Methanolic Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dpph Radical Scavenging Activity ◽  
Leaf Extracts ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Introduction and Aim: Plants are considered to be novel source of active compounds having pharmacological properties and help in the development of therapeutic agents. Hence, this study was undertaken to evaluate the antioxidant, anti-inflammatory, analgesic and anti-proliferating activity of aqueous and methanolic leaf extracts of Grewia heterotricha Mast. Materials and Methods: The aqueous and methanolic leaf extracts of the plant were assessed for their in-vitro antioxidant activity by DPPH radical scavenging activity, in-vivo anti-inflammatory activity by carrageenan induced rat paw edema method, in-vivo analgesic activity by acetic acid-induced writhing test and in-vitro anti- proliferating activity by MTT assay. Results: The methanolic extract had shown very significant DPPH radical scavenging activity with IC50 value 98.95?g/ml than aqueous extract and showed a significant reduction in the paw volume of rats at the concentration of 100 mg/kg body weight indicating potent anti-inflammatory activity compared with the reference standard Diclofenac sodium. Both the extracts showed significant analgesic effect (p<0.001) in acetic acid-induced pain models in a dose dependent manner. The methanolic extract showed higher analgesic activity compared to aqueous extract by inhibiting the pain indicated by a decrease in the number of writhes. In addition, both the extracts showed a decrease in MCF-7 cell viability at the concentration of 550µg/ml. Compared to the aqueous extract, MEGH has shown more cytotoxic effect on the cancer cell lines. Conclusion: The results suggest that both aqueous and methanolic extracts of Grewia heterotricha Mast. leaves possess potent antioxidant, analgesic, anti-inflammatory and anti-proliferating properties, which supports the use of the plant in traditional medicine. Further investigation is required to illuminate on its active compounds.   Keywords: Analgesic; anti-inflammatory; DPPH; cytotoxic.

Preliminary Phytochemical Screening, In Vitro Antioxidant Activity, Topical and Oral Formulation of Extract of Woodfordia fructicosa and Gardenia gummifera

2017 ◽  
Vol 8 ◽  
pp. 16-26
Author(s):  
Hemant K. Nagar ◽  
Mahendra S. Ranawat
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Oral Formulation ◽  
Dpph Method ◽  
Anti Oxidant ◽  
Sedimentation Volume ◽  
Dpph Radical Scavenging

Woodfordia fructicosa and Gardenia gummifera are traditionally claimed to be useful in treatment of number of diseases. The main aim of this study was to evaluate preliminary phytochemical tests and antioxidant activity of ethanolic extract of Woodfordia fructicosa flowers and Gardenia gummifera leaves and finally extracts were formulated in Gels and Suspensions. In vitro antioxidant activity was evaluated by DPPH method. The different concentration (100, 200, 300, 400 and 500 μg/ml) of standard and test samples were prepared. The anti-oxidant activity is exhibited in percentage inhibition. The DPPH radical scavenging activity of both the extracts increases with increasing concentration. The prepared gels were evaluated physical appearance, homogeneity, Grittiness, Spreadability and Viscosity. The prepared suspensions were evaluated organoleptic properties, pH and Sedimentation Volume.

scholarly journals In Vitro Anti-Inflammatory, Anti-Oxidant, and Cytotoxic Activities of Four Curcuma Species and the Isolation of Compounds from Curcuma aromatica Rhizome

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 799 ◽  
Author(s):  
Aknarin Pintatum ◽  
Wisanu Maneerat ◽  
Emilie Logie ◽  
Emmy Tuenter ◽  
Maria E. Sakavitsi ◽  
...  
Keyword(s):  
Crude Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cytotoxic Activities ◽  
Ic50 Value ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Diphenyl Tetrazolium Bromide ◽  
Necrosis Factor

The genus Curcuma is part of the Zingiberaceae family, and many Curcuma species have been used as traditional medicine and cosmetics in Thailand. To find new cosmeceutical ingredients, the in vitro anti-inflammatory, anti-oxidant, and cytotoxic activities of four Curcuma species as well as the isolation of compounds from the most active crude extract (C. aromatica) were investigated. The crude extract of C. aromatica showed 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity with an IC50 value of 102.3 μg/mL. The cytotoxicity effect of C. aeruginosa, C. comosa, C. aromatica, and C. longa extracts assessed with the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 200 μg/mL were 12.1 ± 2.9, 14.4 ± 4.1, 28.6 ± 4.1, and 46.9 ± 8.6, respectively. C. aeruginosa and C. comosa presented apoptosis cells (57.7 ± 3.1% and 32.6 ± 2.2%, respectively) using the CytoTox-ONE™ assay. Different crude extracts or phytochemicals purified from C. aromatica were evaluated for their anti-inflammatory properties. The crude extract of C. aromatica showed the highest potential to inhibit NF-κB activity, followed by C. aeruginosa, C. comosa, and C. longa, respectively. Among the various purified phytochemicals curcumin, germacrone, curdione, zederone, and curcumenol significantly inhibited NF-κB activation in tumor necrosis factor (TNF) stimulated HaCaT keratinocytes. Of all compounds, curcumin was the most potent anti-inflammatory.

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