Interleukin 6 im Liquor – diagnostische Wertigkeit

2015 ◽  
Vol 39 (6) ◽  
Author(s):  
Sylvia Gruber ◽  
Philipp Werner ◽  
Reinhard Germann ◽  
Peter Fraunberger
Keyword(s):  
B Cell ◽  
Interleukin 6 ◽  
Diagnostische Wertigkeit ◽  
Entzündliche Erkrankungen ◽  
Chronisch Entzündliche ◽  
Stimulatory Factor

Zusammenfassung:Interleukin 6 (IL-6) wurde bereits 1985 als Differenzierungsfaktor für B-Zellen (B-cell stimulatory Factor 2) identifiziert, unter dessen Einfluss B-Zellen in Antikörper-bildende Zellen maturierten. In zahlreichen Studien wurde seither der pleiotrope Charakter von IL-6 belegt, das neben seinen Funktionen in der Immunabwehr, auch eine bedeutende Rolle in der Regulation von Hämatopoese, Inflammation und Onkogenese übernimmt. In Bezug auf das ZNS wurde die Beteiligung von IL-6 an der Neurogenese sowie an der Reaktion von Neuronen und Glia-Zellen auf Verletzungen beschrieben. Infektionen des ZNS, cerebrale Ischämien, schwere ZNS-Traumata oder chronisch entzündliche Erkrankungen wie Neuro-Lupus oder Neuro-Sarkoidose gehen mit erhöhten IL-6 Konzentrationen im Liquor einher. Aufgrund der Präsenz des Neuropoetins IL-6 im Rahmen dieser Erkrankungen wird ein möglicher Einsatz des Parameters IL-6 im Liquor zur Diagnostik und, in manchen Fällen, zur Verlaufsbeurteilung und Therapieüberwachung überprüft. In diesem Review geben wir einen Überblick über die derzeitige Studienlage und die diagnostische Wertigkeit von IL-6 im Liquor.

scholarly journals Interleukin-1 accelerates autocrine growth of myeloma cells through interleukin-6 in human myeloma

Blood ◽  
1989 ◽  
Vol 73 (8) ◽  
pp. 2145-2148 ◽  
Author(s):  
M Kawano ◽  
H Tanaka ◽  
H Ishikawa ◽  
M Nobuyoshi ◽  
K Iwato ◽  
...  
Keyword(s):  
B Cell ◽  
Mrna Expression ◽  
Interleukin 6 ◽  
Messenger Rna ◽  
Interleukin 1 ◽  
Autocrine Growth ◽  
Myeloma Cells ◽  
Stimulatory Factor ◽  
Culture Supernatants

Abstract Recombinant interleukin 1 alpha (rIL-1 alpha) augmented proliferation of freshly isolated myeloma cells as well as B-cell stimulatory factor 2 (BSF-2)/interleukin-6 (IL-6). Recombinant IL-1 alpha-induced proliferation was partially inhibited by anti-IL-6 antibody. In the culture supernatants of rIL-1 alpha-stimulated myeloma cells, IL-6 activities, which were measured by using an IL-6-dependent murine hybridoma clone, MH60.BSF2, were increased, when compared with those in the culture supernatants of nonstimulated myeloma cells. Furthermore, IL-6 messenger RNA (mRNA) expression was also augmented in IL-1 alpha- stimulated myeloma cells. Therefore rIL-1 alpha stimulates myeloma cells to produce IL-6, which consequently augments proliferation of myeloma cells. Thus, IL-1 can accelerate autocrine growth of myeloma cells through IL-6.

scholarly journals Regulation of interleukin-6 expression in cultured human blood monocytes and monocyte-derived macrophages

Blood ◽  
1988 ◽  
Vol 72 (4) ◽  
pp. 1134-1140 ◽  
Author(s):  
J Bauer ◽  
U Ganter ◽  
T Geiger ◽  
U Jacobshagen ◽  
T Hirano ◽  
...  
Keyword(s):  
Molecular Weight ◽  
B Cell ◽  
Human Blood ◽  
Interleukin 6 ◽  
Interferon Beta ◽  
Interleukin 2 ◽  
Mononuclear Phagocytes ◽  
Blood Monocytes ◽  
Beta 2 ◽  
Stimulatory Factor

Abstract A culture system that allows human blood monocytes to differentiate into macrophages in vitro was used to study B-cell stimulatory factor- 2/interleukin-6 (interferon-beta 2/26 kd protein) expression in mononuclear phagocytes. Using B-cell stimulatory factor-2 (BSF-2) cDNA and a polyclonal, monospecific antibody directed against human BSF-2, we find that strong interleukin-6 (IL-6) expression is initiated in cultured monocytes on stimulation with endotoxin. Maximally induced monocytic BSF-2/IL-6 synthesis (1% to 2% of total proteins secreted by monocytes) is more than ten times stronger than in terminally differentiated macrophages (approximately 0.1% of total secretory proteins). BSF-2/IL-6 mRNA was detectable as early as one hour after stimulation with endotoxin, reaching maximum levels three hours after stimulus. Interleukin-1 (IL-1) was able to stimulate IL-6 synthesis in monocytes, but not in macrophages. Tumor necrosis factor, interferon- gamma and interleukin-2 (IL-2) had no effect on IL-6 synthesis in monocytes or macrophages. We found five molecular weight forms of BSF- 2/IL-6 to be secreted by monocytes of 21.5 kd, 23.5 kd, 24 kd, 26 kd, and 28 kd apparent molecular weight. The 26 kd and 28 kd forms were found to represent N-glycosylated molecules, which were not detectable on treatment of the cells with the N-glycosylation inhibitor tunicamycin. The 21.5 kd, 23.5 kd, and 24 kd BSF-2/IL-6 forms were unaffected by tunicamycin treatment. We conclude from our data that cells of the mononuclear phagocyte lineage are one of the main sites of BSF-2/IL-6 (interferon-beta 2/26 kd protein/HSF) synthesis.

Constitutive production of interleukin 6/B cell stimulatory factor-2 from inflammatory synovium

1989 ◽  
Vol 52 (2) ◽  
pp. 238-247 ◽  
Author(s):  
Nobuyuki Miyasaka ◽  
Kazuto Sato ◽  
Junko Hashimoto ◽  
Hitoshi Kohsaka ◽  
Kazuhiko Yamamoto ◽  
...  
Keyword(s):  
B Cell ◽  
Interleukin 6 ◽  
Constitutive Production ◽  
Stimulatory Factor

scholarly journals Constitutive expression of the interleukin-6 gene in chronic lymphocytic leukemia

Blood ◽  
1989 ◽  
Vol 73 (5) ◽  
pp. 1279-1284
Author(s):  
A Biondi ◽  
V Rossi ◽  
R Bassan ◽  
T Barbui ◽  
S Bettoni ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chronic Lymphocytic Leukemia ◽  
B Cell ◽  
Interleukin 6 ◽  
Lymphoblastic Leukemia ◽  
Cdna Probe ◽  
Constitutive Expression ◽  
Lymphocytic Leukemia ◽  
Biologically Active ◽  
Stimulatory Factor

Interleukin-6 (IL-6) is a pleiotropic lymphokine active as a growth factor on B-cell hybridomas and plasmacytomas and found to be identical with B-cell stimulatory factor 2, interferon beta 2, 26-Kd protein, and hepatocytes stimulating factor. IL-6 gene expression was investigated in fresh human chronic lymphocytic leukemia (B-CLL) and in acute lymphoblastic leukemia (ALL) by Northern blot analysis using a specific cDNA probe. 1.3-kb IL-6 transcript was found in six out of 11 B-CLL patients, while no hybridization was observed in ten cases of ALL of both T- and B-cell origin. The constitutive expression of IL-6 transcripts was associated with production of a biologically active protein as determined by using the IL-6-dependent 7TD1 cell line. It remains to be elucidated whether IL-6 gene expression is indeed important in the regulation of B-CLL growth or in its clinical manifestation.

Molecular Cloning of the cDNAs for Interleukin-6/B Cell Stimulatory Factor 2 and Its Receptora

2008 ◽  
Vol 557 (1) ◽  
pp. 167-180 ◽  
Author(s):  
T. HIRANO ◽  
T. TAGA ◽  
K. YAMASAKI ◽  
T. MATSUDA ◽  
K. YASUKAWA ◽  
...  
Keyword(s):  
B Cell ◽  
Molecular Cloning ◽  
Interleukin 6 ◽  
Stimulatory Factor

Production of B cell stimulatory factor-2/interleukin-6 activity by human endothelial cells

1988 ◽  
Vol 153 (3) ◽  
pp. 1045-1050 ◽  
Author(s):  
Kenichi Norioka ◽  
Masako Hara ◽  
Masayoshi Harigai ◽  
Atsushi Kitani ◽  
Tatsuo Hirose ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
B Cell ◽  
Interleukin 6 ◽  
Human Endothelial Cells ◽  
Stimulatory Factor
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