Changes of Antioxidant Enzyme and Phenylalanine Ammonia-Lyase Activities during Chimonanthus praecox Seed Maturation

2008 ◽  
Vol 63 (7-8) ◽  
pp. 569-573 ◽  
Author(s):  
Xiaojia He ◽  
Shun Gao
Keyword(s):  
Superoxide Dismutase ◽  
Protein Content ◽  
Antioxidant Enzyme ◽  
Gel Electrophoresis ◽  
Growth And Development ◽  
Phenylalanine Ammonia Lyase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Maturation ◽  
Pal Activity ◽  
Chimonanthus Praecox

Changes in peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and phenylalanine ammonia-lyase (PAL) activities were studied during Chimonanthus praecox seed maturation. According to our findings the protein content increased steadily from 8 to 12 weeks after flowering, and thereafter decreased significantly. Similarly, SOD and POD activities increased gradually up to 12 weeks after flowering and then declined. PAL activity declined gradually during seed maturation. CAT activity, however, showed no changes during seed maturation. By means of polyacrylamide gel electrophoresis (PAGE), SOD and POD isoenzymes were observed during seed maturation. The staining intensities of SOD and POD isoenzymes correlated well with SOD and POD activities as obtained by an assay in solution. These findings suggest that POD, SOD and PAL may be involved in the growth and development during Chimonanthus praecox seed maturation.

Expressing activator protein Ap36 inBacillus thuringiensisand the function of the recombined strain on disease resistance

2008 ◽  
Vol 5 (2) ◽  
pp. 121-126
Author(s):  
Peng Dong-Hai ◽  
Zhou Chen-Fei ◽  
Qiu De-Wen ◽  
Zhou Kang ◽  
Ruan Li-Fang ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Recombinant Strain ◽  
Phenylalanine Ammonia Lyase ◽  
Protein Gene ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Elicitor ◽  
Sds Page ◽  
Rot Disease ◽  
Derivative Strain

AbstractThe geneap36encoding a protein elicitor fromAlternariasp. was fused downstream of theslh(S-layer homology) motif ofBacillus thuringiensisS-layer protein genectc. The recombinant gene was then transferred intoB. thuringiensisplasmid-free derivative strain BMB171. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that the SLH–Ap36 fusion protein was expressed inB. thuringiensisBMB171. After tomato (Lycopersicum esculentum) leaves were treated for 90 min with the recombinant strain cultured at 28°C for 24 h, the activity of peroxidase and the amount of proline of tomato leaves were increased to 57.14% and 131.59%, respectively, compared to the control, and after the tomato leaves were treated with the cultured recombinant strain for 4 days, the activity of phenylalanine ammonia lyase was also higher than that in the control. Furthermore, tubers of treated potato (Solanum tuberosum) plants showed higher resistance to rot disease caused byErwinia corotovoraSCG1 compared to the control treatments.

Effects of PGPR (Pseudomonas sp.) and Ag-nanoparticles on Enzymatic Activity and Physiology of Cucumber

2020 ◽  
Vol 11 (2) ◽  
pp. 124-136 ◽  
Author(s):  
Sehrish Nawaz ◽  
Asghari Bano
Keyword(s):  
Superoxide Dismutase ◽  
Pseudomonas Putida ◽  
Ag Nanoparticles ◽  
Phenylalanine Ammonia Lyase ◽  
Combined Treatment ◽  
Pseudomonas Stutzeri ◽  
Plant Growth Promoting Rhizobacteria ◽  
Vegetative Phase ◽  
Pal Activity ◽  
Plant Growth Promoting

Background: The present investigation aimed to evaluate the role of Plant Growth- Promoting Rhizobacteria (PGPR) and Ag-nanoparticles on two varieties (American variety, Poinsett 76 and Desi variety, Sialkot selection) of cucumber plants. Methods: Cucumber seeds prior to sowing, were inoculated with two strains of PGPR, Pseudomonas putida (KX574857) and Pseudomonas stutzeri (KX574858) at the rate of 106 cells/ml. Agnanoparticles (5ppm) were sprayed on the plant at early vegetative phase 27 d after sowing. Results: The proline, sugar, protein, phenolics, flavonoids, chlorophyll and carotenoids contents of leaves of plants and the activities of Phenylalanine Ammonia-Lyase (PAL), Superoxide Dismutase (SOD) and Catalase (CAT) were determined from leaves of plants at early vegetative phase. After 3 months of seeds sowing, Ag-nanoparticles enhanced the length of root but decreased the length of shoot and fresh weight of root and shoot as compared to control whereas, the leaf protein, proline, phenolics, flavonoids, chlorophyll b, total chlorophyll, sugar and Phenylalanine Ammonia-Lyase (PAL) activity of plants were increased significantly over control. Ag-nanoparticles also suppressed the effect of PGPR for root, shoot length but augmented the protein and phenolics contents of leaves of both the varieties. Conclusion: The combined treatment of Ag-nanoparticles and PGPR enhanced flavonoids content of leaves and the activities of PAL, SOD and CAT in leaves of plants over control. Agnanoparticles effectively increased the Phenylalanine Ammonia-Lyase (PAL), Catalase (CAT) and superoxide dismutase (SOD) activities in leaves of both the varieties. Pseudomonas putida may be used either alone or in combination with Ag-nanoparticles to enhance the antioxidant and defense enzyme activities. Hence, the plant can tolerate the diseases and stresses in a much better way with higher protein and phenolics content.

Superoxide dismutase activity in nematodes

1987 ◽  
Vol 61 (3) ◽  
pp. 229-232 ◽  
Author(s):  
M. Sanchez-Moreno ◽  
P. Leon ◽  
M. A. Garcia-Ruiz ◽  
M. Monteoliva
Keyword(s):  
Superoxide Dismutase ◽  
Catalase Activity ◽  
Gel Electrophoresis ◽  
Species Differences ◽  
Pattern Analysis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Toxocara Canis ◽  
Adult Males ◽  
Males And Females ◽  
Toxascaris Leonina

ABSTRACTThe activities of the enzymes superoxide dismutase (E.C.:1.15.1.1) and catalase (E.C: 1.11.1.6) were studied in purified extracts of four nematodes: Ascaris suum, Toxascaris leonina, Toxocara canis and T. cati adult males and females. No catalase activity was found in any of the extracts. The results reveal that the SOD activities of the four parasites presented species differences and also sexual differences within each species. Polyacrylamide gel electrophoresis pattern analysis confirmed that the mobilities, widths and band intensities varied according to the species and sex of the parasite from which the enzyme was obtained.

Variability of the Seed Globulins of Winged Bean, Psophocarpus tetragonolobus (L.) DC

1978 ◽  
Vol 5 (3) ◽  
pp. 371 ◽  
Author(s):  
RJ Blagrove ◽  
JM Gillespie
Keyword(s):  
Protein Content ◽  
Papua New Guinea ◽  
Gel Electrophoresis ◽  
Protein Pattern ◽  
Polyacrylamide Gel Electrophoresis ◽  
Psophocarpus Tetragonolobus ◽  
Electrophoretic Patterns ◽  
Pure Lines ◽  
Seed Globulins ◽  
Single Subunit

Extracts from the seeds of 80 pure lines of P. tetragonolobus found in Papua New Guinea have been examined by polyacrylamide gel electrophoresis, There was little difference between the electrophoretic patterns except for variation in the proportion of one polypeptide. In its unreduced form, this polypeptide is the single subunit of psophocarpin A. A number of these genotypes have been studied more closely to obtain information regarding their protein content and the influence of extraction conditions on the protein pattern observed.

scholarly journals Effects of copper on growth, antioxidant enzymes and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedling

2008 ◽  
Vol 54 (No. 3) ◽  
pp. 117-122 ◽  
Author(s):  
S. Gao ◽  
R. Yan ◽  
M. Cao ◽  
W. Yang ◽  
S. Wang ◽  
...  
Keyword(s):  
Protein Content ◽  
Jatropha Curcas ◽  
Phenylalanine Ammonia Lyase ◽  
Gradual Increase ◽  
Shoot Elongation ◽  
Copper Stress ◽  
Sod Activity ◽  
Pal Activity ◽  
Seedling Biomass ◽  
Leaves And Roots

The effects of different concentrations of copper (0–800 &mu;mol) on growth, protein contents, peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), and phenylalanine ammonia-lyase (PAL) in <I>Jatropha curcas</I> L. seedlings were assessed by means of pot experiments. Results suggested that increased copper concentrations lead to decreased shoot elongation and seedling biomass. Protein content in the leaves and roots reached their highest levels at the copper concentrations of 400 &mu;mol, while the highest protein content in the stem was observed at 800 &mu;mol copper. POD activity in leaves and stems was unaffected at low copper concentrations, but showed a considerable variation at high copper concentrations. In roots, the highest POD activity was observed at 200 &mu;mol copper. Under copper stress, SOD activity in leaves increased concomitantly with increasing copper up to 400 &mu;mol, and SOD activity in stems and roots showed a slight increase. Catalase activity significantly elevated in leaves and roots but showed no significant changes in stems of the seedlings exposed to copper. A gradual increase of PAL activity in leaves and roots at the copper concentration of 400 and 200 &mu;mol was observed, while PAL activity remained unchanged in stems.

Protein and lipid methylation by methionine and S-adenosylmethionine in Myxococcus xanthus

1983 ◽  
Vol 29 (9) ◽  
pp. 1224-1228 ◽  
Author(s):  
Sharon M. Panasenko
Keyword(s):  
Protein Synthesis ◽  
Vegetative Growth ◽  
Gel Electrophoresis ◽  
Growth And Development ◽  
Polyacrylamide Gel Electrophoresis ◽  
Myxococcus Xanthus ◽  
Polyacrylamide Gel ◽  
Methyl Donors

Methylation of lipids and proteins has been examined in Myxococcus xanthus using radioactive methionine and S-adenosylmethionine as methyl donors. S-adenosylmethionine is shown to be taken up by these cells and utilized directly. This permits detection of methylation in the presence of protein synthesis. Patterns of methylation obtained using methionine and S-adenosylmethionine during vegetative growth are compared by polyacrylamide gel electrophoresis, and inhibitors of protein synthesis and S-adenosylmethionine synthesis are examined for their effects on methylation. The ability to investigate methylation using exogenous S-adenosylmethionine will be advantageous in studying the role of methylation under conditions of growth and development where ongoing protein synthesis is required.

An inducible iron-containing superoxide dismutase in Rhizobium japonicum

1981 ◽  
Vol 27 (11) ◽  
pp. 1202-1208 ◽  
Author(s):  
Mark D. Stowers ◽  
Gerald H. Elkan
Keyword(s):  
Superoxide Dismutase ◽  
Electrophoretic Mobility ◽  
Gel Electrophoresis ◽  
Metal Content ◽  
Methyl Viologen ◽  
Polyacrylamide Gel Electrophoresis ◽  
Rhizobium Japonicum ◽  
Growth Media ◽  
Sod Activity ◽  
Rhizobium Spp

Crude extracts of Rhizobium japonicum contained a single superoxide dismutase (SOD) as revealed by polyacrylamide gel electrophoresis. Rhizobium japonicum SOD was determined to be iron-containing by criteria of sensitivity to 5 mM H2O2 and 1 mM NaN3. Growth media strongly influenced SOD activity. The addition of methyl viologen to cultures of R. japonicum 61A76NS, growing in complex medium, caused induction of the enzyme. Yeast extract was essential for induction of SOD. Puromycin blocked the induction of the enzyme. Rhizobium spp. were surveyed for SOD activity, electrophoretic mobility on polyacrylamide gels, and metal content.

Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen

2018 ◽  
Vol 43 (4) ◽  
pp. 435-446
Author(s):  
Aydan Acar Şahin ◽  
Belma Aslım ◽  
Sema Tan ◽  
Şenol Alan ◽  
Nur Münevver Pınar
Keyword(s):  
Protein Content ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Pectate Lyase ◽  
Polyacrylamide Gel ◽  
Pollen Allergen ◽  
Allergenic Protein ◽  
Diagnosis And Therapy ◽  
Cupressus Arizonica

Abstract Objective Cupressaceae pollen has commonly been reported to be an important aeroallergen and causal factor of spring, autumn and winter pollinosis in many countries. The aim of this study was to compare of the structure and allergenic protein content of Cupressus arizonica Greene., Cupressus sempervirens L. and Juniperus oxycedrus L. pollen in detail and contribute to Cupressaceae pollen allergen diagnosis and therapy studies in Turkey. Methods The pollen structure were examined by LM and SEM. Pollen protein content was investigated by Bradford protein assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot analysis and two-dimensional polyacrylamide gel electrophoresis (2DE PAGE), respectively. Pectate lyase (PL) enzyme activities were compared. Immunoblotting was carried out by using extracts of the three taxa pollen collected from Turkey. Results All three taxa was found very similar in terms of pollen morphology however, intine thickness was prominently different. Cupressus arizonica pollen extracts showed the lowest PL activity. Five sera specific IgE of all allergic subjects showed reaction with only C. arizonica pollen extracts. Conclusions As a conclusion, the pollen structure, protein function or protein structure and isoforms of allergens could affects allergenic properties of the pollen. This study also may help to improve the Cupressaceae pollen allergen diagnosis and therapy.

scholarly journals ANATOMICAL AND BIOCHEMICAL ASPECTS OF THEVETIA PERUVIANA L. A COMMONLY PLANTED ROADSIDE TROPICAL SHRUB OF BHUBANESWAR, ODISHA

2017 ◽  
Vol 9 (5) ◽  
pp. 51
Author(s):  
Jyoti Ranjan Rout ◽  
Rout George Kerry ◽  
Lilyprava Dash ◽  
Soumya Ranjan Nayak ◽  
Santi Lata Sahoo
Keyword(s):  
Air Pollution ◽  
Antioxidant Enzyme ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Carotenoid Content ◽  
Leaf Extracts ◽  
Thevetia Peruviana ◽  
The Impact ◽  
Roadside Plants

Objective: Air pollution is one of the most severe environmental problems of developing cities which adversely affects both plant and human life. However, roadside plants in the urban locations help in reducing the pollution level from the air through foliar surface and act as pollution sink. In this work, an attempt was made to understand the impact of air pollution on urban roadside plants which act as bio indicator.Methods: A study was conducted to check the impact of urbanization on air pollution by analyzing anatomical and biochemical aspects of Thevetia peruviana L. For anatomical analysis, the transverse sections of leaves were observed under a microscope; whereas for biochemical analysis, the estimation of chlorophyll, carotenoid and protein were determined by spectrophotometric methods. On the other hand, antioxidant enzyme assays, as well as specific activity staining, were performed to study the antioxidant potential. To investigate the antioxidant activity of the leaf extracts, an assay for catalase (CAT), guaiacol peroxidase (GPX) and superoxide dismutase (SOD) were performed. Isoforms of CAT, GPX and SOD were separated using native polyacrylamide gel electrophoresis (PAGE) and their activities were analyzed.Results: Analysis of data revealed that the anatomical disturbances significantly occurred in exposed plants of various sites. Among biochemical parameters, photosynthetic pigments such as chlorophyll a, chlorophyll b, total chlorophyll and carotenoid content were found to be decreased. A maximum (7.65±0.51, 3.78±0.56,11.43±0.91, 5.63±0.55 mg/g, respectively) decrement was noticed in leaves of the Pokhariput plant in comparison to Khorda NH5 (19.44±1.08, 10.12±1.03, 29.56±2.32, 10.22±1.21 mg/g, respectively) which is taken as control one. To determine the effect of air pollution on proteins, leaf extracts were analyzed by the Lowry method and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The substantial decrement of total protein and alteration in polypeptides were noticed in polluted sites. Meanwhile, the enhancement of antioxidant enzyme activities such as CAT, GPX and SOD were also detected.Conclusion: The increment of antioxidant enzymes and alteration of proteins, suggesting the activation of defensive mechanisms in selected plants under air pollution stress and also the plants made physiological and biochemical adjustments to overcome the oxidative damage.

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