scholarly journals Analysis of the essential oil composition of three cultivated Nepeta species from Iran

2020 ◽  
Vol 75 (7-8) ◽  
pp. 247-254 ◽  
Author(s):  
Fatemeh Zahra Amirmohammadi ◽  
Majid Azizi ◽  
Seyyed Hossein Nemati ◽  
Marcello Iriti ◽  
Sara Vitalini

AbstractEssential oils (EOs) of three Iranian cultivated Nepeta species were investigated. The oils were obtained by hydrodistillation of air-dried plant materials at full flowering stage and analyzed by gas chromatography (GC) and gas chromatography coupled to mass spectroscopy (GC/MS). In total, 89 compounds were detected. In over 2 years, a number of constituents were identified in the EO of Nepeta binaloudensis first and second years (26 and 37, respectively), Nepeta cataria (25 and 32, respectively), and Nepeta assurgens (45 and 50, respectively). In the oils of N. binaloudensis, 4a-α,7-α,7a-α-nepetalactone (NL) 59.7% and 1,8-cineole (19.6%) during the first and second years, respectively, were the main constituents. The main components of N. cataria were 4a-α,7-α,7a-β-NL (72.8%) and 4a-α,7-β,7a-α-NL (73.9%) during the first and second years, respectively, and 4a–α,7-α,7a-α-NL (55.5%) and 1,8-cineole (24.1%) during the first and second years, respectively, were the main constituents of N. assurgens. The results showed that NLs isomers and 1,8-cineole were the main components of the oils of three cultivated Nepeta species.

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Avni Hajdari ◽  
Behxhet Mustafa ◽  
Dashnor Nebija ◽  
Hyrmete Selimi ◽  
Zeqir Veselaj ◽  
...  

The principal aim of this study was to analyze the chemical composition and qualitative and quantitative variability of essential oils obtained from seven naturally grown populations of thePinus peuceGrisebach, Pinaceae in Kosovo. Plant materials were collected from three populations in the Sharri National Park and from four other populations in the Bjeshkët e Nemuna National Park, in Kosovo. Essential oils were obtained by steam distillation and analyzed by GC-FID (Gas Chromatography-Flame Ionization Detection) and GC-MS (Gas Chromatography-Mass Spectrometry). The results showed that the yield of essential oils (v/wdry weight) varied depending on the origin of population and the plant organs and ranged from 0.7 to 3.3%. In total, 51 compounds were identified. The main compounds wereα-pinene (needles: 21.6–34.9%; twigs: 11.0–24%),β-phellandrene (needles: 4.1–27.7; twigs: 29.0–49.8%), andβ-pinene (needles: 10.0–16.1; twigs: 6.9–20.7%). HCA (Hierarchical Cluster Analysis) and PCA (Principal Component Analyses) were used to assess geographical variations in essential oil composition. Statistical analysis showed that the analyzed populations are grouped in three main clusters which seem to reflect microclimatic conditions on the chemical composition of the essential oils.


2017 ◽  
Vol 42 (6) ◽  
Author(s):  
Masomeh Alimohammadi ◽  
Mehrab Yadegari ◽  
Hamze Ali Shirmardi

AbstractObjective:Determine the best elevation and phenological stages effects on essential oil content and composition inMaterials and methods:Three phenological stages (vegetative, full flowering and seeding) and three elevation ranges (2500–2700, 2700–2900 and over 2900 m), shoots of plants collected from Kallar mountain as natural habitats from Chaharmahal and Bakhtiari Province. Composition of essential oil detected by GC/MS.Results:The maximum amount of secondary metabolites of β-ocimene, methyl chavicol, germacrene-D, 1,8 cineole and phthalate had measured in vegetative stage and various of elevation ranges. The most essential oil content (0.145%) was obtained in 2700–2900 m and full flowering stage. The most components in full flowering were β-eudesmol, menthol and γ-eudesmol. The most of germacrene-D, levomenol, β-thujene, β-caryophyllene, β-sesquiphellandrene, α-eudesmol and delta-cadinene was achieved in seeding stage.Conclusion:Elevation and phenological stages had significant effect on essential oil of


2017 ◽  
Vol 45 (1) ◽  
pp. 185-189 ◽  
Author(s):  
Ozlem TONCER ◽  
Sengul KARAMAN ◽  
Emel DIRAZ ◽  
Tahsin SOGUT ◽  
Suleyman KIZIL

Thymus is represented by 39 species (60 taxa) in Turkey and the rate of its endemism is 45% in Turkey. The herb is widely used for several purposes. Its essential oil compositions and biologically active substances are affected by the phenological stages. Therefore, the aim of this study is to evaluate the variations in the essential oil composition of Thymus × citriodorus (Pers.) Schreb. growing in Southeastern Anatolia Region of Turkey at different phenological stages (pre-flowering, full flowering, post flowering). The oils were obtained by hydrodistillation of air-dried samples. The yields of oils (w/w %) at different phenological stages was respectively 2.0% at pre-flowering state, 1.9% at flowering stage, and 1.3% at post-flowering stage. T. × citriodorus was determined mainly by using GC/MS. A total of 21 compounds, representing 93.9% in the pre-flowering stage, 98.8% in the flowering stage and 98.91% in post-flowering of the total oil, were identified. Monoterpene hydrocarbons (terpinolene) and oxygenated monoterpenes (α-terpineol) were observed to have the highest percentage in T. × citriodorus oil. The major compounds of the oil were terpinolene, α- terpineol, linalool, bornyl acetate and borneol. The highest percentage of terpinolene was determined to be 71% in flowering stages.


2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Jesús Palá-Paúl ◽  
Jaime Usano-Alemany ◽  
Joseph J. Brophy ◽  
María J. Pérez-Alonso ◽  
Ana-Cristina Soria

The essential oils from the different parts [inflorescences (E.a.I), stems + leaves (E.a.SL) and roots (E.a.R)] of E. aquifolium Cav. gathered in Cádiz (Spain), have been extracted by steam distillation and analyzed by gas chromatography and gas chromatography coupled to mass spectrometry. Quantitative and qualitative differences have been found between the analyzed plant parts. A total of 107 compounds have been identified. The main constituents were germacrene D (30.3%) and sesquicineole (26.7%) for E.a.I fraction, germacrene D (46.0%) and myrcene (13.8%) in the E.a.SL, while E.a.R showed phyllocladene isomer (63.6%) as a unique major compound. The percentage composition of the other constituents was lower than 5.5% in all the analyzed fractions. In agreement with other Eryngium species, no specific compound could be used as a marker for the chemotaxonomy of E. aquifolium. However, similarities in volatile composition were found between E. aquifolium and other species growing under similar environmental conditions. As far as we know, this is the first report on the essential oil of this species.


2016 ◽  
Vol 11 (10) ◽  
pp. 1934578X1601101
Author(s):  
Kaan Polatoğlua ◽  
Betül Demirci ◽  
İhsan Çalιş ◽  
Kemal Hüsnü Can Başer

The essential oil of aerial parts of Helichrysum conglobatum (Viv.) Steudel. (Asteraceae) from Cyprus was analyzed by GC and GC-MS. The essential oil yield was 0.01, v/w. Forty five compounds were identified in the oil comprising 96.1% of the total. The essential oil was mainly composed of sesquiterpene type compounds and oxygenated sesquiterpene derivatives. The main components of the oil were β-caryophyllene (14.6%), γ-curcumene (14.1%), hexadecanoic acid (13.5%), tetradecanoic acid (7.5%), rosifoliol (5.4%) and δ-cadinene (5.3%). This is the first report on the essential oil composition of H. conglobatum from Cyprus.


Biomolecules ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 879
Author(s):  
Ahmed E. Altyar ◽  
Mohamed L. Ashour ◽  
Fadia S. Youssef

The metabolic variation in the essential oil composition of Premna odorata leaves obtained from different seasons was quantitatively and qualitatively determined employing GC/MS (Gas Chromatography coupled with Mass Spectrometry) and GC/FID (Gas chromatography equipped with flame ionization detector) techniques. It displayed the existence of 97 constituents accounting for 94.19%, 92.27%, 91.95% and 92.63% for POS (spring), POM (summer), POA (autumn) and POW (winter) whole essential oils. β-Caryophyllene constituting the main metabolite in the oil in the different seasons. To better visualize the differences between them, GC data were exposed to chemometric analysis. A PCA (principal component analysis) score plot revealed the closeness of POS and POW. Molecular modelling on collagenase, elastase and hyaluronidase enzymes active centres shows that different compounds existing in the essential oil of Premna odorata leaves shows binding to the active sites with variable degrees that suggested its anti-ageing potential. Palmitic acid displayed the highest fitting for both the collagenase and elastase active centres in both pH-based and rule-based ionization methods with ∆G equals −78.27 and −44.77 kcal/mol, respectively; meanwhile, heptacosane showed the highest fitting score in the hyaluronidase centre with ∆G = −43.78 kcal/mol. In vitro assays consolidates the obtained modelling studies in which essential oil shows considerable anti-elastase and anti-hyaluronidase potential as evidenced by their IC50 values being 49.3 and 37.7 μg/mL, respectively; meanwhile, the essential oil of Premna odorata leaves displayed mild anti-collagenase potential. Thus, it can be concluded that Premna odorata could serve as a promising anti-ageing naturally occurring drug that could be effectively incorporated by pharmaceutical industries in cosmetics combating ageing and skin wrinkling.


2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Isiaka A. Ogunwande ◽  
Razaq Jimoh ◽  
Adedoyin A. Ajetunmobi ◽  
Nudewhenu O. Avoseh ◽  
Guido Flamini

Essential oils obtained by hydrodistillation of leaves of two Nigerian species were analyzed for their constituents by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The leaf oil of Ficus benjamina L. (Moraceae), collected during the day, contained high contents of α-pinene (13.9%), abietadiene (9.7%), cis-α-bisabolene (8.2%) and germacrene-D-4-ol (8.4%), while the night sample was dominated by germacrene-D-4-ol (31.5%), 1,10-di- epi-cubenol (8.8%) and hexahydrofarnesylacetone (8.3%). This could be a possible indication of differences in emissions of volatiles by F. benjamina during the day and night. The main compounds of Irvingia barteri Hook. f. (Irvingiaceae) were β-caryophyllene (17.0%), (E)-α-ionone (10.0%), geranial (7.6%), (E)-β-ionone (6.6%) and β-gurjunene (5.1%).


2007 ◽  
Vol 2 (1) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Hossein Nazemiyeh ◽  
Seied M. Razavi ◽  
Abbas Delazar ◽  
Rogaieh Hajiboland ◽  
Valiollah Mozaffarian ◽  
...  

The composition of the essential oils of the umbels and fruit of Prangos uloptera, an indigenous Iranian medicinal plant, was determined by GC-MS analyses. A total of 10 and 18 compounds were identified, respectively, from the essential oils of the umbels and the fruit. The identified compounds represented, respectively, 93.2% and 83.0% of the total essential oils. While the main components of the umbels oil were α-pinene (31.78%), β-bourbonene (15.9%), α-curcumene (10.65%), spathulenol (9%) and m-cymene (5.51%), those of the fruit oil were α-pinene (14.98%), β-bourbonene (7.81%), α-humulene (7.74%), germacrene B (7.23%) and n-tetracosane (6.65%).


2016 ◽  
Vol 11 (3) ◽  
pp. 1934578X1601100
Author(s):  
Rajendra C. Padalia ◽  
Ram S. Verma ◽  
Amit Chauhan ◽  
Prakash Goswami ◽  
Chandan S. Chanotiya

The leaf essential oil composition of Taxodium distichum L., collected from the foothills of Uttarakhand, India was analyzed using gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS) equipped with DB-5 (5% diphenyl-95% dimethyl polysiloxane) and β-cyclodextrin (6-tertiarybutyldimethylsiliyl-2,3-diethyl-β-cyclodextrin) capillary columns. Seventeen constituents, representing 90.3 to 99.4% of composition were identified in the essential oils from different seasons, viz. spring, summer, rainy, autumn and winter. The essential oil composition was mainly dominated by monoterpene hydrocarbons, represented mainly by α-pinene (81.9–94.3%). Other constituents of the oil were myrcene (0.5–4.7%), β-pinene (2.2–2.9%), limonene (0.5–1.5%), camphene (≤0.03–1.5%), and α-terpineol (upto 1.6%). Chiral analysis of T. dstichum essential oil on an ethyl substituted β-cyclodextrin capillary column revealed the presence of α-pinene in racemic form, with an enantiomeric ratio of 49.3% for (1 R)-(+)- and 50.7% for (1 S)-(-)-α-pinene.


2013 ◽  
Vol 8 (3) ◽  
pp. 1934578X1300800
Author(s):  
Rajesh K. Joshi

The essential oil composition from the aerial parts of Baccharoides lilacina (Dalzell & A. Gibson) M. R. Almeida was analyzed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). A total of 41 compounds have been identified, representing 97.4% of the total oil. The main constituents were identified as β-caryophyllene (27.7%), epi-α-cadinol (25.1%), caryophyllene oxide (9.9%), α-muurolol (7.6%), α-cadinene (6.1%) and α-cadinol 4.5%). The oil was found to be rich in oxygenated sesquiterpenes (47.1%) and sesquiterpene hydrocarbons (46.2%).


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