scholarly journals Comparative Metabolomics Analysis of Juvenile Takifugu obscurus serum and Liver Samples after Exposure to Tributyltin Chloride

Author(s):  
Dong-Po Xu ◽  
Di-An Fang ◽  
Shu-Lun Jiang ◽  
Chang-Sheng Zhao
2018 ◽  
Author(s):  
Xu Dong-po ◽  
Fang Di-an ◽  
Zhao Chang-sheng ◽  
Jiang Shu-lun ◽  
Hu Hao-yuan

AbstractHSP90β1 (known as GP96) is a vital endoplasmic reticulum depended chaperonin among the HSPs family. It plays important roles in regulating the growth, development, differentiation, and apoptosis of cells. Furthermore, it always processes and presents antigen of the tumor and keeps balance for the intracellular environment. In the present study, we explored the effect of tributyltin chloride (TBT-Cl) exposure on HSP90β1 expression in river pufferfish, Takifugu obscurus. The full length of To-HSP90β1 was gained with 2775 bp in length, an ORF obtained with 2412 bp encoding an 803 aa polypeptide. The phylogenetic tree was constructed and showed the close relationship to other fish species. The HSP90β1 mRNA transcript was expressed in all tissues investigated. After the acute and chronic exposure of TBT-Cl, the mRNA level of To-HSP90β1 significantly up-regulated in tissues of liver and gill. Moreover, the histochemistry study indicated the injury degree of TBT-Cl on liver and gill. Immunohistochemistry (IHC) staining results implied the cytoplasm reorganization after TBT-Cl stress and the function of immunoregulation for To-HSP90β1. All the results indicated that HSP90β1 may involve in the resistance to the invasion of TBT-Cl for keeping autoimmune homeostasis.


2020 ◽  
Vol 40 (12) ◽  
Author(s):  
Rong Gong ◽  
Daoqiang Huang ◽  
Yibo Chen ◽  
Hong Li ◽  
Zhidong Wang ◽  
...  

AbstractGood eating quality is a highly desirable trait of rice which determines its commercial value and market share. However, the molecular basis of this trait remains largely unknown. Here, three high-quality conventional rice cultivars, including two superior eating quality cultivars Meixiangzhan-2 (MXZ) and Xiangyaxiangzhan (XYXZ), and one ordinary eating quality cultivar Huanghuazhan (HHZ), were analyzed by comparative metabolomics to identify the inherent mechanism for the formation of superior eating quality. The results showed 58.8% of common differential substances between MXZ vs HHZ and XYXZ vs HHZ were enriched in MXZ and XYXZ, whereas 39.2% of them were prominently decreased compared with HHZ, mainly including amino acids, carbohydrates, lipids, phenolamides, and flavonoids, which may be the primary factors leading to the differences of taste and flavor among these three cultivars. We also found that lysine derivatives and fatty acids may have a close relationship with taste. These results above provide important insights into the taste-forming mechanism of rice and will be beneficial for superior eating quality rice breeding.


Gene ◽  
2019 ◽  
Vol 688 ◽  
pp. 84-92 ◽  
Author(s):  
Di-An Fang ◽  
Chang-Sheng Zhao ◽  
Shu-Lun Jiang ◽  
Yan-Feng Zhou ◽  
Dong-Po Xu

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