scholarly journals β-galactosidase is involved in rice seed germination

Author(s):  
Qi Zhang ◽  
Yuanxuan Peng ◽  
Xiumei Li ◽  
Bingxian Chen ◽  
Jun Liu

The purpose of this study was to explore the relationship between β-galactosidase and rice seed germination. Exogenous β-galactosidase (1 U) facilitated germination while abscisic acid (ABA) delayed germination. Histo­chemical staining and spectrophotometry revealed that the activity of endogenous β-galactosidase increased gradually during germination. 1 µM ABA and GA4+7 could, respectively, inhibit and promote the activity of β-galactosidase in the rice embryo. This indicated that the regulation of rice seed germination by these two phytohormones may be through mediation of β-galactosidase activity. During seed germination, the transcriptional expressions of OsBAGL1, OsBAGL4, OsBAGL8 and OsBAGL11 were higher than those of other homologous genes, suggesting that these four genes may be involved in the germination process. GA4+7 significantly increased the expression of the above four genes. By contrast, the transcriptional expression of OsBAGL8 and OsBAGL11 were significantly reduced by ABA, but not that of OsBAGL1 and OsBAGL4. Our study has important theoretical and practical significance for illustrating the relationship between β-galactosidase and seed germination.

2019 ◽  
Vol 20 (2) ◽  
pp. 450 ◽  
Author(s):  
Rebecca Damaris ◽  
Zhongyuan Lin ◽  
Pingfang Yang ◽  
Dongli He

Alpha-amylase, the major form of amylase with secondary carbohydrate binding sites, is a crucial enzyme throughout the growth period and life cycle of angiosperm. In rice, alpha-amylase isozymes are critical for the formation of the storage starch granule during seed maturation and motivate the stored starch to nourish the developing seedling during seed germination which will directly affect the plant growth and field yield. Alpha-amylase has not yet been studied intensely to understand its classification, structure, expression trait, and expression regulation in rice and other crops. Among the 10-rice alpha-amylases, most were exclusively expressed in the developing seed embryo and induced in the seed germination process. During rice seed germination, the expression of alpha-amylase genes is known to be regulated negatively by sugar in embryos, however positively by gibberellin (GA) in endosperm through competitively binding to the specific promoter domain; besides, it is also controlled by a series of other abiotic or biotic factors, such as salinity. In this review, we overviewed the research progress of alpha-amylase with focus on seed germination and reflected on how in-depth work might elucidate its regulation and facilitate crop breeding as an efficient biomarker.


2018 ◽  
Vol 1031 ◽  
pp. 119-127 ◽  
Author(s):  
Hua-Ming Xiao ◽  
Wen-Jing Cai ◽  
Tian-Tian Ye ◽  
Jun Ding ◽  
Yu-Qi Feng

EMJ Radiology ◽  
2020 ◽  
Author(s):  
Filippo Pesapane

Radiomics is a science that investigates a large number of features from medical images using data-characterisation algorithms, with the aim to analyse disease characteristics that are indistinguishable to the naked eye. Radiogenomics attempts to establish and examine the relationship between tumour genomic characteristics and their radiologic appearance. Although there is certainly a lot to learn from these relationships, one could ask the question: what is the practical significance of radiogenomic discoveries? This increasing interest in such applications inevitably raises numerous legal and ethical questions. In an environment such as the technology field, which changes quickly and unpredictably, regulations need to be timely in order to be relevant.  In this paper, issues that must be solved to make the future applications of this innovative technology safe and useful are analysed.


BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Qi Wu ◽  
Yiming Luo ◽  
Xiaoyong Wu ◽  
Xue Bai ◽  
Xueling Ye ◽  
...  

Abstract Background Night-break (NB) has been proven to repress flowering of short-day plants (SDPs). Long-noncoding RNAs (lncRNAs) play key roles in plant flowering. However, investigation of the relationship between lncRNAs and NB responses is still limited, especially in Chenopodium quinoa, an important short-day coarse cereal. Results In this study, we performed strand-specific RNA-seq of leaf samples collected from quinoa seedlings treated by SD and NB. A total of 4914 high-confidence lncRNAs were identified, out of which 91 lncRNAs showed specific responses to SD and NB. Based on the expression profiles, we identified 17 positive- and 7 negative-flowering lncRNAs. Co-expression network analysis indicated that 1653 mRNAs were the common targets of both types of flowering lncRNAs. By mapping these targets to the known flowering pathways in model plants, we found some pivotal flowering homologs, including 2 florigen encoding genes (FT (FLOWERING LOCUS T) and TSF (TWIN SISTER of FT) homologs), 3 circadian clock related genes (EARLY FLOWERING 3 (ELF3), LATE ELONGATED HYPOCOTYL (LHY) and ELONGATED HYPOCOTYL 5 (HY5) homologs), 2 photoreceptor genes (PHYTOCHROME A (PHYA) and CRYPTOCHROME1 (CRY1) homologs), 1 B-BOX type CONSTANS (CO) homolog and 1 RELATED TO ABI3/VP1 (RAV1) homolog, were specifically affected by NB and competed by the positive and negative-flowering lncRNAs. We speculated that these potential flowering lncRNAs may mediate quinoa NB responses by modifying the expression of the floral homologous genes. Conclusions Together, the findings in this study will deepen our understanding of the roles of lncRNAs in NB responses, and provide valuable information for functional characterization in future.


Apeiron ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Jan Maximilian Robitzsch

Abstract This paper examines the classification of desires that the Epicureans offer in their writings. It surveys the extant textual evidence for the classification and discusses the relationship between natural and necessary, natural and unnecessary, and unnatural and unnecessary desires. It argues that while the practical significance of the Epicurean classification is clear, which desires fall into which class is not. The paper suggests the reason for this may be that the Epicureans acknowledge some variability in their concept of human nature, arguing for a functional reading of the Epicurean classification of desires.


2012 ◽  
Vol 524-527 ◽  
pp. 2474-2481
Author(s):  
Zhi Gang Huang ◽  
Jiao Ling Xie ◽  
Wen Ping Wu

Carbon emissions permits has its own particularity,and with the development of carbon finance,carbon emissions permits possess the commodity attributes and financial attributes.So its price isn’t determined only by the relationship of commodity supply and demand,but also affected by a variety of factors.But because the transaction data is not available,so the pricing of the carbon emissions permits can not really consider from the angle of the influencing factors of price.Therefore, this paper is on the basis of previous studies using mathematical tools and introducing the option pricing mechanism to study th pricing of China's carbon emissions permits basing on carbon emissions,which is designed for providing reference on the pricing of China's carbon emissions,being of both theoretical and practical significance.


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