Comparison of methionyl human growth hormone and pituitary growth hormone on somatic growth of hypophysectomized rats

1984 ◽  
Vol 107 (2) ◽  
pp. 192-198 ◽  
Author(s):  
A. Skottner ◽  
A. Forsman ◽  
E. Löfberg ◽  
K.-G. Thorngren
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Bone Growth ◽  
Biological Effects ◽  
Human Growth ◽  
Male Rats ◽  
Cartilage Growth ◽  
Human Pituitary ◽  
Longitudinal Bone Growth ◽  
Pituitary Growth Hormone

Abstract. In hypophysectomized male rats the biological effects of two batches of methionyl human growth hormone, Somatonorm®, (met-hGH), have been compared to those of human pituitary growth hormone, Crescormon® (hGH). The rats were treated with doses ranging from 10 mIU per day to 145 mIU per day for 10 days. The parameters studied were total weight gain, longitudinal bone growth, measured by the tetracycline method and indirect cartilage growth, measured by uptake of radioactive sulphate. The results obtained demonstrated that Somatonorm® stimulated weight increase in a linear and dose-dependent way, similar to that seen with the native hormone. Longitudinal bone growth, measured by the tetracycline method and the growth of different cartilages, measured as uptake of radioactive sulphate, were also similar between the two hormones.

BIOASSAY OF GROWTH HORMONE AND PROLACTIN PREPARATIONS BY DETERMINATION OF LONGITUDINAL BONE GROWTH WITH TETRACYCLINE

1974 ◽  
Vol 76 (1) ◽  
pp. 35-52 ◽  
Author(s):  
K.-G. Thorngren ◽  
L. I. Hansson
Keyword(s):  
Growth Hormone ◽  
Present Method ◽  
Bone Growth ◽  
Biological Effects ◽  
Human Growth ◽  
Lower Growth ◽  
Longitudinal Bone Growth ◽  
Hypophysectomized Rats ◽  
Growth Promoting

ABSTRACT The growth stimulating effect of different growth hormone and prolactin preparations on the longitudinal bone growth in thyroxine-treated hypophysectomized rats was determined by the tetracycline method. The effect of the hormone preparations was compared with that of the 1st International Standard for growth hormone. The potency calculation showed that the tested human growth hormone preparations have a higher potency than the bovine, ovine and porcine growth hormone preparations. Also potency differences were found between hormones from the same species but prepared by different methods. The prolactin preparations have a considerably lower growth promoting activity than the growth hormone preparations. The bioassay method used in the present investigation has a favourable mean precision (λ = 0.172) and sensitivity compared with the earlier bioassay methods. The present method increases the possibility of determining the biological effects of various growth promoting substances.

RADIOAUTOGRAPHIC STUDIES ON THE LOCALIZATION OF 125I-LABELLED HUMAN LUTEINIZING AND GROWTH HORMONE IN IMMATURE MALE RATS

1969 ◽  
Vol 43 (1) ◽  
pp. 105-111 ◽  
Author(s):  
D. M. DE KRETSER ◽  
K. J. CATT ◽  
H. G. BURGER ◽  
G. C. SMITH
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Luteinizing Hormone ◽  
Human Growth Hormone ◽  
Light Microscope ◽  
Human Growth ◽  
Proximal Convoluted Tubule ◽  
Male Rats ◽  
Immature Male ◽  
Parenchymal Cells

SUMMARY Twenty-day-old male rats were injected intraperitoneally with either human luteinizing hormone (HLH) or human growth hormone (HGH) labelled with 125I. The localization of these hormones 1–2 hr. after injection was examined under the light microscope after radioautography. Major sites of localization of labelled LH were the interstitial cells of the testis and the proximal convoluted tubule of the kidney. Some hormone was also present in adipose tissue, hepatic parenchymal cells, the mesothelial lining of the peritoneum and underlying macrophages. HGH was localized principally in the proximal convoluted tubule of the kidney with some hormone present in liver, adipose tissue, and the suprarenal cortex.

BONE GROWTH AND METABOLIC STUDIES OF PREMATURE INFANTS TREATED WITH HUMAN GROWTH HORMONE

PEDIATRICS ◽  
1965 ◽  
Vol 36 (6) ◽  
pp. 836-842
Author(s):  
G. Chiumello ◽  
A. Vaccari ◽  
F. Sereni
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Premature Infants ◽  
Bone Growth ◽  
Metabolic Response ◽  
Nitrogen Retention ◽  
Human Growth ◽  
Urinary Hydroxyproline ◽  
Hormone Administration ◽  
Hydroxyproline Excretion

The influence of exogenous human growth hormone on growth and metabolism of premature infants was observed. It was not possible to register any increase of linear bone growth after hormone administration; concomitantly urinary hydroxyproline excretion did not change significantly. Nitrogen balance studies indicated a sharp increase of nitrogen retention, due to a reduced urinary excretion, in all infants. Calcium and phosphorus balances rose in three out of four premature infants treated with growth hormone, but the characteristic STH calciuric action of STH was not observed. Furthermore, STH failed to induce any significant increase in NEFA serum concentration of premature infants. It may therefore be concluded that the metabolic response of premature infants to STH differs consistently from that normally observed in more mature subjects.

Human growth hormone in cultures of human pituitary tumors

1971 ◽  
Vol 34 (6) ◽  
pp. 741-748 ◽  
Author(s):  
Ulrich Batzdorf ◽  
Vivian Gold ◽  
Nancy Matthews ◽  
Josiah Brown
Keyword(s):  
Growth Hormone ◽  
Tissue Culture ◽  
Human Growth Hormone ◽  
Culture Medium ◽  
Pituitary Tumors ◽  
Human Growth ◽  
Tissue Culture Medium ◽  
Human Pituitary ◽  
Content Type ◽  
Juvenile Diabetic

✓ Tissue culture medium of 14 human pituitary adenomas removed from acromegalic and hypopituitary patients was analyzed for human growth hormone (HGH). High HGH levels were detected in the culture medium of all adenomas removed from acromegalic patients. HGH was undetectable in the tissue culture medium of six of eight adenomas removed from hypopituitary patients. Elevated HGH levels were also found in the culture medium of pituitary tissue obtained from a juvenile diabetic patient.

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