scholarly journals clc-2c is regulated by salinity, prolactin and extracellular osmolality in tilapia gill

2017 ◽  
Vol 59 (4) ◽  
pp. 391-402 ◽  
Author(s):  
Jason P Breves ◽  
Paige L K Keith ◽  
Bethany L Hunt ◽  
K Keano Pavlosky ◽  
Mayu Inokuchi ◽  
...  
Keyword(s):  
Danio Rerio ◽  
External Environment ◽  
Oreochromis Mossambicus ◽  
Local Effect ◽  
Intrinsic Factors ◽  
Mozambique Tilapia ◽  
Regulatory Link ◽  
Gill Filaments ◽  
Ovine Prolactin ◽  
First Time

Teleosts inhabiting fresh water (FW) depend upon ion-absorptive ionocytes to counteract diffusive ion losses to the external environment. A Clc Cl−channel family member, Clc-2c, was identified as a conduit for basolateral Cl−transport by Na+/Cl−cotransporter 2 (Ncc2)-expressing ionocytes in stenohaline zebrafish (Danio rerio). It is unresolved whether Clc-2c/clc-2cis expressed in euryhaline species and how extrinsic and/or intrinsic factors modulate branchialclc-2cmRNA. Here, we investigated whether environmental salinity, prolactin (Prl) and osmotic conditions modulateclc-2cexpression in euryhaline Mozambique tilapia (Oreochromis mossambicus). Branchialclc-2candncc2mRNAs were enhanced in tilapia transferred from seawater (SW) to FW, whereas both mRNAs were attenuated upon transfer from FW to SW. Next, we injected hypophysectomized tilapia with ovine prolactin (oPrl) and observed a marked increase inclc-2cfrom saline-injected controls. To determine whether Prl regulatesclc-2cin a gill-autonomous fashion, we incubated gill filaments in the presence of homologous tilapia Prls (tPrl177and tPrl188). By 24 h, tPrl188stimulatedclc-2cexpression ~5-fold from controls. Finally, filaments incubated in media ranging from 280 to 450 mosmol/kg for 3 and 6 h revealed that extracellular osmolality exerts a local effect onclc-2cexpression;clc-2cwas diminished by hyperosmotic conditions (450 mosmol/kg) compared with isosmotic controls (330 mosmol/kg). Our collective results suggest that hormonal and osmotic control of branchialclc-2ccontributes to the FW adaptability of Mozambique tilapia. Moreover, we identify for the first time a regulatory link between Prl and a Clc Cl−channel in a vertebrate.

scholarly journals Prolactin 177, prolactin 188, and extracellular osmolality independently regulate the gene expression of ion transport effectors in gill of Mozambique tilapia

2015 ◽  
Vol 309 (10) ◽  
pp. R1251-R1263 ◽  
Author(s):  
Mayu Inokuchi ◽  
Jason P. Breves ◽  
Shunsuke Moriyama ◽  
Soichi Watanabe ◽  
Toyoji Kaneko ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Incubation Time ◽  
Oreochromis Mossambicus ◽  
Mrna Levels ◽  
Ion Transporters ◽  
Local Effects ◽  
Mozambique Tilapia ◽  
Gill Filaments ◽  
Euryhaline Teleost

This study characterized the local effects of extracellular osmolality and prolactin (PRL) on branchial ionoregulatory function of a euryhaline teleost, Mozambique tilapia ( Oreochromis mossambicus). First, gill filaments were dissected from freshwater (FW)-acclimated tilapia and incubated in four different osmolalities, 280, 330, 380, and 450 mosmol/kg H2O. The mRNA expression of Na+/K+-ATPase α1a (NKA α1a) and Na+/Cl− cotransporter (NCC) showed higher expression with decreasing media osmolalities, while Na+/K+/2Cl− cotransporter 1a (NKCC1a) and PRL receptor 2 (PRLR2) mRNA levels were upregulated by increases in media osmolality. We then incubated gill filaments in media containing ovine PRL (oPRL) and native tilapia PRLs (tPRL177 and tPRL188). oPRL and the two native tPRLs showed concentration-dependent effects on NCC, NKAα1a, and PRLR1 expression; Na+/H+ exchanger 3 (NHE3) expression was increased by 24 h of incubation with tPRLs. Immunohistochemical observation showed that oPRL and both tPRLs maintained a high density of NCC- and NKA-immunoreactive ionocytes in cultured filaments. Furthermore, we found that tPRL177 and tPRL188 differentially induce expression of these ion transporters, according to incubation time. Together, these results provide evidence that ionocytes of Mozambique tilapia may function as osmoreceptors, as well as directly respond to PRL to modulate branchial ionoregulatory functions.

scholarly journals Effects of 17ß-estradiol Hormone on Growth and Sex Differentiation in the Mozambique Tilapia (Oreochromis mossambicus, Peters, 1852)

2019 ◽  
Vol 34 (1) ◽  
pp. 14-21
Author(s):  
Karl Christofer Kingueleoua Koyakomanda ◽  
Muamer Kürşat Fırat ◽  
Cüneyt Süzer ◽  
Serhat Engin ◽  
Müge Hekimoğlu ◽  
...  
Keyword(s):  
Sex Differentiation ◽  
Oreochromis Mossambicus ◽  
Mozambique Tilapia

Recovery Effect of a Rutin-Enriched Fraction Prepared From Forsythia koreana Flowers on Alloxan-Induced Pancreatic Islets in Zebrafish Larvae (Danio rerio)

2021 ◽  
Vol 16 (10) ◽  
pp. 1934578X2110437
Author(s):  
Yeong-Geun Lee ◽  
Youn Hee Nam ◽  
Jung Eun Gwag ◽  
Jung-Hwan Ko ◽  
Kyeong-Hwa Seo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pancreatic Islets ◽  
Danio Rerio ◽  
Spectroscopic Data ◽  
Recovery Effect ◽  
Meoh Extract ◽  
Simple Method ◽  
Zebrafish Larvae ◽  
Forsythia Koreana ◽  
First Time

A simple method to prepare a high-content rutin fraction from Forsythia koreana flowers (HRFK) is described. Rutin, isolated for the first time from the flowers, was identified from spectroscopic data including nuclear magnetic resonance, mass spectrometry, and infrared. As a result of HPLC quantitative analysis, the content of rutin was found to be 80.0 ± 0.02% in HRFK. Our previous study reported that F koreana MeOH extract (FK) significantly recovered alloxan-induced pancreatic islets in zebrafish. However, HRFK as well as rutin exhibited an enhanced anti-diabetic effect compared to FK in our latest experiments. In conclusion, HRFK, as well as rutin and FK, have potential as anti-diabetic agents.

ESTIMATION OF NITROGLYCERINE WATER SOLUTION TOXICITY BY HISTOLOGICAL AND CYTOLOGICAL INDICES USING DANIO RERIO AS A CASE STUDY

Fisheries ◽  
2020 ◽  
Vol 2020 (1) ◽  
pp. 66-70
Author(s):  
Yuri Simakov ◽  
Dmitry Nikiforov-Nikishin ◽  
Larisa Bychkova ◽  
Nadegda Lyubovskaya
Keyword(s):  
Danio Rerio ◽  
Laboratory Experiments ◽  
Maximum Permissible Concentration ◽  
Water Solution ◽  
Water Bodies ◽  
Hematological Indices ◽  
First Time ◽  
Pharmaceutical Enterprises

The results of laboratory experiments on nitroglycerine toxicity detected by histological and cytological indices are presented, using Danio rerio as a case study. For the first time, there are shown the changes in liver and kidneys, both at tissular and cellular levels, induced by administered concentrations of nitroglycerine. The results obtained appear to be important for water bodies’ preservation and elaboration of MPC standards. The intake of nitroglycerine into water bodies is due, mainly, to discharge from pharmaceutical enterprises, demolition works, and outflow from explosives storehouses. Fish turned out to be sensitive to nitroglycerine as indicated by histological and hematological indices. Maximum permissible concentration, MPC, for Danio rerio is determined to be equal to 0.5 mg/l.

scholarly journals Anaerobic digestion is the dominant pathway for pit latrine decomposition and is limited by intrinsic factors

2019 ◽  
Vol 79 (12) ◽  
pp. 2242-2250 ◽  
Author(s):  
Miriam H. A. van Eekert ◽  
Walter T. Gibson ◽  
Belen Torondel ◽  
Faraji Abilahi ◽  
Bernard Liseki ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Oxygen Demand ◽  
In Vitro Tests ◽  
Full Potential ◽  
Water Addition ◽  
Intrinsic Factors ◽  
Pit Latrine ◽  
First Time

Abstract In vitro methods were used to assess the full potential for decomposition (measured as biogas formation) from pit latrine samples taken from the top layer of 15 Tanzanian latrines. We found considerable variability in the decomposition rate and extent. This was compared with decomposition in the same latrines, measured by comparing top layer composition with fresh stools and deeper (older) layers, to assess whether this potential was realised in situ. Results showed a close match between the extent of organic material breakdown in situ and in vitro, indicating that anaerobic digestion is the dominant pathway in latrines. The average potential decrease in chemical oxygen demand (COD) (determined as methane production in vitro within 60 days) and actual measured decrease in situ are 68.9% ± 11.3 and 69.7% ± 19.4, respectively. However in the in vitro tests, where samples were diluted in water, full decomposition was achieved in 2 months, whereas in situ it can take years; this suggests that water addition may offer a simple route to improving latrine performance. The results also allowed us to estimate, for the first time to our knowledge using experimental data, the contribution that latrines make to greenhouse gas emissions globally. This amounts to ∼2% of annual US emissions.

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