scholarly journals Development of a specific method to evaluate 8-hydroxy,2-deoxyguanosine in sperm nuclei: relationship with semen quality in a cohort of 94 subjects

Reproduction ◽  
2013 ◽  
Vol 145 (3) ◽  
pp. 227-235 ◽  
Author(s):  
M Cambi ◽  
L Tamburrino ◽  
S Marchiani ◽  
B Olivito ◽  
C Azzari ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Oxidative Damage ◽  
Oxidative Dna Damage ◽  
Semen Quality ◽  
Sperm Count ◽  
Specific Method ◽  
Sperm Dna Fragmentation ◽  
Immunofluorescence Method ◽  
Sperm Nuclei ◽  
The Relationship

Oxidative stress (OS) is involved in many disoders including male infertility. Human spermatozoa are very sensitive targets of reactive oxygen species (ROS) and most sperm functions are impaired in the case of OS. In addition unbalanced production of ROS is considered one of the most important causes of sperm DNA fragmentation, a semen trait of infertile men. The relationship between oxidative damage and semen quality is partially controversial, probably due to the different methods and/or targets used to reveal the OS. In this study, by fluorescence microscopy and flow cytometry, we compared two methods to reveal 8-hydroxy,2-deoxyguanosine (8-OHdG), the hallmark of oxidative DNA damage: an immunofluorescence method and the commercial OxyDNA kit. We found that although both methods localized the labelling in sperm nuclei they yielded different measures, and only with the immunofluorescence method was the labelling specific for sperm 8-OHdG. The immunofluorescence method, coupled to flow cytometry, was thus selected to analyse the 8-OHdG content in semen samples from 94 subfertile patients and to investigate the relationship with semen quality. We found that the percentages of spermatozoa with 8-OHdG (mean±s.d., 11.4±6.9%) were related to sperm count (Pearson's correlation coefficient (r)=−0.27, P=0.04 (ANOVA and student's t-test)), motility (progressive: r=−0.22, P=0.04; non-progressive: r=0.25, P=0.01), and normal morphology (r=−0.27, P=0.01). In conclusion, we demonstrate that immunofluorescence/flow cytometry is a reliable and specific method to detect 8-OHdG at single-cell level and show that oxidative damage only partially overlaps poor semen quality, suggesting that it could provide additional information on male fertility with respect to routine semen analysis.

scholarly journals Semen quality and diversity of morphological sperm abnormalities in bulls: breed and strain effects

2019 ◽  
Vol 22 (8) ◽  
pp. 931-938
Author(s):  
M. A. Kleshchev ◽  
V. L. Petukhov ◽  
L. V. Osadchuk
Keyword(s):  
Sperm Motility ◽  
Environmental Conditions ◽  
Western Siberia ◽  
Semen Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Sperm Head ◽  
Sperm Dna Fragmentation ◽  
Sperm Production ◽  
Sperm Abnormalities

At present great attention is paid to studying genetic regulation of farm animal adaptations to environmental conditions. This problem is very important due to a wide expansion of highly productive cattle breeds created in Europe and North America. However, until the present no investigation of changing semen quality in bulls of imported breeds during their adaptations to environmental conditions of Western Siberia has been conducted. The aim of this study was to investigate semen quality peculiarities and the diversity of morphological sperm abnormalities in bulls of imported and local breeds kept in the environmental conditions of the southern part of Western Siberia. We determined sperm concentration, sperm count, and rate of sperm with progressive motility and percentage of morphologically normal spermatozoa. The rate of sperm abnormalities according to Blome’s classifcation was determined too. It was found that the mean values of sperm concentration, sperm motility and percentage of morphologically normal spermatozoa in the bulls investigated were similar to those in bulls kept in European countries. Inter­breed differences in these parameters were not found. However, bulls of the Red Danish, Angler, and Simmental breeds had a higher percentage of misshapen sperm head and pyriform sperm head than bulls of the Black­White breed. An inter­strain difference in sperm motility in bulls of the Black­White breed was observed. It was found that bulls of Reflection Sovereign 198998 strain had lover sperm motility than bulls of Wis Burke Ideal 1013415 strain. No inter­strain differences in sperm production, percentage of morphologically normal spermatozoa and rate of main sperm abnormalities were found. Thus, it has been found that the environmental conditions of the southern part of Western Siberia do not seriously affect the sperm production, sperm motility or percentage of morphologically normal spermatozoa in bulls. However, the increased rate of misshapen and pyriform sperm heads in the bulls of the foreign breeds points to a need to study sperm DNA fragmentation.

Relationship between semen quality and seminal plasma cholesterol, triacylglycerols and proteins in the domestic cat

2019 ◽  
Vol 22 (10) ◽  
pp. 882-889 ◽  
Author(s):  
María F García ◽  
Romina Nuñez Favre ◽  
María C Stornelli ◽  
Ramiro Rearte ◽  
María C García Mitacek ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Sperm Morphology ◽  
Semen Quality ◽  
Sperm Count ◽  
Sodium Dodecyl ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Domestic Cat ◽  
Sds Page ◽  
The Relationship

Objectives The current study aimed to evaluate the relationship between specific seminal plasma components – cholesterol (CHOL), triacylglycerols (TAG) and total protein (PROT) concentrations – and semen quality in cats. A further aim was to determine the relationship between specific seminal protein bands and semen quality. Methods Thirteen toms, 2–5 years of age, were included. Semen collection was performed by electroejaculation every 4 weeks. Fifty-eight ejaculates were assessed for motility, velocity, volume, sperm concentration, total sperm count, viability, acrosome integrity, plasma membrane integrity and sperm morphology. Samples were divided into two groups: good semen quality (GSQ) and poor semen quality (PSQ). After evaluation, seminal plasma was separated from the sperm by centrifugation and stored at −20°C. CHOL, TAG and PROT concentrations were then assessed and seminal plasma protein profile was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Results Seminal plasma CHOL and TAG concentrations, motility, velocity, sperm concentration, total sperm count and sperm morphology were significantly higher in GSQ cats compared with PSQ cats ( P <0.01). Moreover, seminal plasma SDS-PAGE analysis showed an identifiable extra band exclusively in the GSQ group. Conclusions and relevance Data obtained in this study showed that seminal plasma CHOL and TAG concentrations and specific protein bands could be used to improve semen evaluation in toms. In this sense, the 14 kDa protein band could be a valuable marker for semen quality in the cat and should be further investigated. However, more studies are necessary to determine its relationship with fertility.

scholarly journals Coenzyme Q10, oxidative stress, and male infertility: A review

2021 ◽  
Vol 48 (2) ◽  
pp. 97-104
Author(s):  
Ahmed T. Alahmar ◽  
Aldo E. Calogero ◽  
Rajender Singh ◽  
Rossella Cannarella ◽  
Pallav Sengupta ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Male Infertility ◽  
Coenzyme Q10 ◽  
Semen Quality ◽  
Sperm Count ◽  
Specific Treatment ◽  
Physiological Role ◽  
Semen Parameters ◽  
Sperm Dna Fragmentation ◽  
Male Reproductive Function

Male infertility has a complex etiopathology, which mostly remains elusive. Although research has claimed that oxidative stress (OS) is the most likely underlying mechanism of idiopathic male infertility, the specific treatment of OS-mediated male infertility requires further investigation. Coenzyme Q10 (CoQ10), a vitamin-like substance, has been found in measurable levels in human semen. It exhibits essential metabolic and antioxidant functions, as well as playing a vital role in mitochondrial bioenergetics. Thus, CoQ10 may be a key player in the maintenance of biological redox balance. CoQ10 concentrations in seminal plasma directly correlate with semen parameters, especially sperm count and sperm motility. Seminal CoQ10 concentrations have been shown to be altered in various male infertility states, such as varicocele, asthenozoospermia, and medical or surgical regimens used to treat male infertility. These observations imply that CoQ10 plays an important physiological role in the maintenance and amelioration of semen quality. The present article thereby aimed to review the possible mechanisms through which CoQ10 plays a role in the regulation of male reproductive function, and to concisely discuss its efficacy as an ameliorative agent in restoring semen parameters in male infertility, as well as its impact on OS markers, sperm DNA fragmentation, pregnancy, and assisted reproductive technology outcomes.

scholarly journals ​Association of Bio-acoustic Features of Vocal Signals with Age and Semen Quality in Sahiwal Bulls

2021 ◽  
Author(s):  
Indu Devi ◽  
Kuldeep Dudi ◽  
Ranjana Sinha ◽  
R. Vikram
Keyword(s):  
Semen Quality ◽  
Sperm Count ◽  
Acoustic Features ◽  
Seminal Parameters ◽  
Sperm Abnormality ◽  
The Mean ◽  
Live Sperm ◽  
The Relationship ◽  
The Voice

Background: Bio-acoustic features of animal’s voice can provide meaningful information about their biological and physical characteristics. The present study was conducted to get indicators of age from voice analysis and explore the relationship between voice features and seminal parameters in Sahiwal bulls. Methods: Voice samples were collected from healthy bulls (n=20), maintained at ICAR-NDRI, Karnal. Bulls were classified into two groups i.e. young bulls and adult bulls. The voice signals were analyzed by Adobe Premium software and acoustic features were extracted by using PRAAT software. Result: The mean of acoustic features viz. call duration (sec), mean intensity (dB), total energy (P2S), amplitude (P), pitch (Hz), unvoiced frame (%), jitter (%), bandwidth (Hz) mean N/H ratio (%) have been found significantly different while mean H/N ratio (dB), shimmer (%) and pulses were not found statistically (P greater than 0.05) different between adult and young bulls. The seminal parameters viz mass activity (0-5 scale), individual progressive motility (%), live sperm count (%) and total sperm abnormality (%) were found significantly different between adult and young bulls. No significant association between voice features and semen quality of bulls was observed. Hence, voice signals of male might provide some clues about their age but for semen quality, there is further need to explore the interesting relationship between voice features and fertility of breeding bulls.

scholarly journals Effect of nut consumption on semen quality and functionality in healthy men consuming a Western-style diet: a randomized controlled trial

2018 ◽  
Vol 108 (5) ◽  
pp. 953-962 ◽  
Author(s):  
Albert Salas-Huetos ◽  
Rocío Moraleda ◽  
Simona Giardina ◽  
Ester Anton ◽  
Joan Blanco ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Fatty Acids ◽  
Semen Quality ◽  
Sperm Count ◽  
Semen Parameters ◽  
Control Group ◽  
Sperm Dna Fragmentation ◽  
Healthy Men ◽  
Randomized Controlled ◽  
Sperm Dna

ABSTRACT Background Human semen quality has declined in industrialized countries. Pollution, smoking, and the consumption of a Western-style diet are all hypothesized as potential causes. Objective We evaluated the effect of chronic consumption of nuts on changes in conventional semen parameters and the potential mechanisms implicated. Design The FERTINUTS study was a 14-wk randomized, controlled, parallel trial. A total of 119 healthy men, aged 18–35 y, were allocated to 1 of 2 intervention groups: one group was fed the usual Western-style diet enriched with 60 g of a mixture of nuts/d (nut group), and the other was fed the usual Western-style diet avoiding nuts (control group). Semen and blood samples were collected at baseline and at the end of the intervention. Dietary information was recorded throughout the trial. Changes in conventional semen parameters (pH, volume, sperm count and concentration, motility, and morphology) were determined as primary outcomes. The effect of nut consumption on sperm DNA fragmentation (SDF), reactive oxygen species (ROS) production, chromosome anomalies (X, Y, and 18), total DNA methylation, and microRNA expression were measured in sperm samples as potential causes of the changes in the seminogram. Results Compared with the control group, improvements in total sperm count (P = 0.002) and vitality (P = 0.003), total motility (P = 0.006), progressive motility (P = 0.036), and morphology of sperm (P = 0.008) were observed in the nut group. Participants in the nut group showed an increase in the consumption of total fat, monounsaturated fatty acids, polyunsaturated fatty acids, magnesium, vitamin E, α-linolenic acid, total omega-3 (n–3) and ω-3:ω-6 ratio intake during the intervention. Participants in the nut group showed a significant reduction in SDF (P < 0.001) and in the expression of hsa-miR-34b-3p (P = 0.036). No significant changes in ROS, sperm chromosome anomalies, or DNA methylation were observed between groups. Conclusions The inclusion of nuts in a Western-style diet significantly improves the total sperm count and the vitality, motility, and morphology of the sperm. These findings could be partly explained by a reduction in the sperm DNA fragmentation. This trial was registered at ISRCTN as ISRCTN12857940.

scholarly journals Effects of Heavy Metal Exposure on Shipyard Welders: A Cautionary Note for 8-Hydroxy-2’-Deoxyguanosine

2019 ◽  
Vol 16 (23) ◽  
pp. 4813 ◽  
Author(s):  
Su ◽  
Pan ◽  
Hsu ◽  
Lai
Keyword(s):  
Oxidative Stress ◽  
Heavy Metal ◽  
Dna Damage ◽  
Oxidative Damage ◽  
Oxidative Dna Damage ◽  
Hazard Identification ◽  
Metal Exposure ◽  
Welding Fumes ◽  
Heavy Metal Exposure ◽  
The Relationship

Oxidative stress plays a crucial role in the development of diseases induced by welding fumes. To our knowledge, little information is available on the relationship between multiple heavy metal exposure and oxidative stress in welders. We assessed the relationship between multiple heavy metal exposure and oxidative damage by analyzing 174 nonsmoking male welders in a shipyard. Urinary metals were used as the internal dose of exposure to metals in welding fumes, and urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG) was used as an oxidative DNA damage marker. The relationship between workers’ metal levels and 8-OHdG was estimated using a multiple linear regression model. The geometric mean levels of urinary chromium (Cr), nickel (Ni), cadmium (Cd), and lead (Pb) were considerably higher in welders than in controls. Urinary Cr and Ni were determined as effective predictors of urinary 8-OHdG levels after adjusting for covariates. Oxidative DNA damage was associated with both Cr and Ni of welding fume exposure in shipyard welders (Ln Cr: β = 0.33, 95%C.I. = 0.16–0.49; Ln Ni: β = 0.27, 95%C.I. = 0.12–0.43). In this study, we investigated the significantly positive relationship between urinary metals (especially Cr and Ni) and 8-OHdG in nonsmoking shipyard welders. Moreover, the use of particulate respirators did not reduce metal exposure and oxidative damage. Therefore, we infer that hazard identification for welders should be conducted.

scholarly journals MiR-125b-2 Knockout in Testis Is Associated with Targeting to the PAP Gene, Mitochondrial Copy Number, and Impaired Sperm Quality

2019 ◽  
Vol 20 (1) ◽  
pp. 148 ◽  
Author(s):  
Longlong Li ◽  
Yanling Zhu ◽  
Ting Chen ◽  
Jiajie Sun ◽  
Junyi Luo ◽  
...  
Keyword(s):  
Copy Number ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Count ◽  
Positive Influence ◽  
Luciferase Assay ◽  
Mtdna Copy Number ◽  
Mitochondrial Copy Number ◽  
Mitochondria Dna ◽  
The Relationship

It has been reported that the miR-125 family plays an important role in regulating embryo development. However, the function of miR-125b-2 in spermatogenesis remains unknown. In this study, we used a model of miR-125b knockout (KO) mice to study the relationship between miR-125b-2 and spermatogenesis. Among the KO mice, the progeny test showed that the litter size decreased significantly (p = 0.0002) and the rate of non-parous females increased significantly from 10% to 38%. At the same time, the testosterone concentration increased significantly (p = 0.007), with a remarkable decrease for estradiol (p = 0.02). Moreover, the sperm count decreased obviously (p = 0.011) and the percentage of abnormal sperm increased significantly (p = 0.0002). The testicular transcriptome sequencing revealed that there were 173 up-regulated genes, including Papolb (PAP), and 151 down-regulated genes in KO mice compared with wild type (WT). The Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) analysis showed that many of these genes were involved in sperm mitochondrial metabolism and other cellular biological processes. Meanwhile, the sperm mitochondria DNA (mtDNA) copy number increased significantly in the KO mice, but there were no changes observed in the mtDNA integrity and mutations of mt-Cytb, as well as the mt-ATP6 between the WT mice and KO mice. In the top 10 up-regulated genes, PAP, as a testis specific expressing gene, affect the process of spermatogenesis. Western blotting and the Luciferase assay validated that PAP was the target of miR-125b-5p. Intriguingly, we also found that both miR-125b and PAP were only highly expressed in the germ cells (GC) instead of in the Leydig cells (LC) and Sertoli cells (SC). Additionally, miR-125b-5p down regulated the secretion of testosterone in the TM3 cell by targeting PAP (p = 0.021). Our study firstly demonstrated that miR-125b-2 regulated testosterone secretion by directly targeting PAP, and increased the sperm mtDNA copy number to affect semen quality. The study indicated that miR-125b-2 had a positive influence on the reproductive performance of animals by regulating the expression of the PAP gene, and could be a potential drugs and diagnostic target for male infertility.

scholarly journals Urinary Bisphenol A, F and S Levels and Semen Quality in Young Adult Danish Men

2021 ◽  
Vol 18 (4) ◽  
pp. 1742
Author(s):  
Thea Emily Benson ◽  
Anne Gaml-Sørensen ◽  
Andreas Ernst ◽  
Nis Brix ◽  
Karin Sørig Hougaard ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Male Fertility ◽  
Parental Education ◽  
Negative Binomial ◽  
Semen Quality ◽  
Sperm Count ◽  
Smoking Status ◽  
Sperm Concentration ◽  
First Trimester ◽  
Negative Binomial Regression Model

Bisphenol A (BPA) is considered an endocrine disruptor and has been associated with deleterious effects on spermatogenesis and male fertility. Bisphenol F (BPF) and S (BPS) are structurally similar to BPA, but knowledge of their effects on male fertility remains limited. In this cross–sectional study, we investigated the associations between exposure to BPA, BPF, and BPS and semen quality in 556 men 18–20 years of age from the Fetal Programming of Semen Quality (FEPOS) cohort. A urine sample was collected from each participant for determination of BPA, BPF, and BPS concentrations while a semen sample was collected to determine ejaculate volume, sperm concentration, total sperm count, sperm motility, and sperm morphology. Associations between urinary bisphenol levels (continuous and quartile–divided) and semen characteristics were estimated using a negative binomial regression model adjusting for urine creatinine concentration, alcohol intake, smoking status, body mass index (BMI), fever, sexual abstinence time, maternal pre–pregnancy BMI, and first trimester smoking, and highest parental education during first trimester. We found no associations between urinary bisphenol of semen quality in a sample of young men from the general Danish population.

DNA fragmentation and morphometric studies in sperm of stallions supplemented with maca (Lepidium meyenii)

Zygote ◽  
2021 ◽  
pp. 1-6
Author(s):  
Emanuele D’Anza ◽  
Sara Albarella ◽  
Giacomo Galdiero ◽  
Simona Tafuri ◽  
Chiara Del Prete ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Breeding Season ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Food Supplementation ◽  
Sperm Dna Fragmentation ◽  
Economic Management ◽  
Lepidium Meyenii ◽  
The Andes ◽  
Length Width

Summary The reproductive performances of livestock play an essential role in the economic management of the farm. The improvement of semen quantity and quality through the use of food supplements that lack substances which are forbidden in animal feeding, or that may have detrimental effects, is an important goal. Maca (Lepidium meyenii) is a plant that has been used for centuries in the Andes for nutrition and fertility enhancement in humans and animals. The aim of this study was to evaluate the effects of food supplementation of stallions with maca during the breeding season on spermatozoa parameters such as DNA fragmentation and shape, which are two predictive indexes of spermatozoa functionality. For this purpose, ejaculate volume, semen gel-free volume, sperm concentration and motility, total sperm count, sperm DNA fragmentation and sperm head parameters (length, width, perimeter, area, shape factor, roughness) were measured in four stallions. Maca food supplementation in stallions during breeding reduced the percentage of spermatozoa with fragmented DNA, increased significantly sperm concentration and exerted an elongation of the spermatozoa head, a condition that is believed to improve spermatozoa functionality, suggesting that food supplementation of maca could be useful in horse breeding during the breeding season.

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