scholarly journals Molecular aspects of bovine cystic ovarian disease pathogenesis

Reproduction ◽  
2015 ◽  
Vol 149 (6) ◽  
pp. R251-R264 ◽  
Author(s):  
Hugo H Ortega ◽  
Belkis E Marelli ◽  
Florencia Rey ◽  
Ayelen N Amweg ◽  
Pablo U Díaz ◽  
...  
Keyword(s):  
Post Partum ◽  
Dairy Farm ◽  
Economic Loss ◽  
Receptor Expression ◽  
Cellular Mechanisms ◽  
Signaling Systems ◽  
Ovarian Disease ◽  
Cystic Ovarian Disease ◽  
Molecular Aspects ◽  
Coregulatory Proteins

Cystic ovarian disease (COD) is one of the main causes of reproductive failure in cattle and causes severe economic loss to the dairy farm industry because it increases both days open in the post partum period and replacement rates due to infertility. This disease is the consequence of the failure of a mature follicle to ovulate at the time of ovulation in the estrous cycle. This review examines the evidence for the role of altered steroid and gonadotropin signaling systems and the proliferation/apoptosis balance in the ovary with cystic structures. This evidence suggests that changes in the expression of ovarian molecular components associated with these cellular mechanisms could play a fundamental role in the pathogenesis of COD. The evidence also shows that gonadotropin receptor expression in bovine cystic follicles is altered, which suggests that changes in the signaling system of gonadotropins could play a fundamental role in the pathogenesis of conditions characterized by altered ovulation, such as COD. Ovaries from animals with COD exhibit a disrupted steroid receptor pattern with modifications in the expression of coregulatory proteins. These changes in the pathways of endocrine action would trigger the changes in proliferation and apoptosis underlying the aberrant persistence of follicular cysts.Free Spanish abstract: A Spanish translation of this abstract is freely available at http://www.reproduction-online.org/content/149/6/R251/suppl/DC1.

scholarly journals Prevalence of Brucellosis in Dairy Cattle in Organized and Smallholder Farms in Some Selected Areas of Bangladesh

2014 ◽  
Vol 12 (2) ◽  
pp. 167-171
Author(s):  
MAS Sarker ◽  
MS Rahman ◽  
MT Islam ◽  
AKMA Rahman ◽  
MB Rahman ◽  
...  
Keyword(s):  
Age Groups ◽  
Dairy Farm ◽  
Economic Loss ◽  
Screening Tests ◽  
Ring Test ◽  
Serum Samples ◽  
Smallholder Farms ◽  
Three Samples ◽  
Holstein Friesian ◽  
Confirmatory Tests

Brucellosis causes a great economic loss to the livestock industries through abortion, infertility,birth of weak and dead offspring, increased calving interval and reduction of milk yield and it is endemic in Bangladesh. In this study we collected milk and blood samples simultaneously from533 cows of Central Cattle Breeding and Dairy Farm, Savar, Dhaka and different Upazilas of Gaibandha and Mymensingh District. Five hundred thirty three samples were examined for antibodies to Brucella using the Milk Ring Test (MRT) and Rose Bengal Test (RBT). Overall 2.62 % of milk samples were positive according to MRT, while2.06 % of the serum samples were positive to the RBT. Only 6 (1.13 %) of the samples were positive for both test (MRT and RBT). Out of 312 samples only 10 (3.20 %) were positive to MRT while 8(2.06%) were positive to RBT in Holstein Friesian cross (p>0.05) on the other hand out of 221 samples only 4 (1.80%) were positive to MRT while 3(1.35%) were positive to RBT in Sahiwal cross. The prevalence of brucellosis was significantly higher in the age group of > 5 years than other age groups (p?0.01) on both test (MRT 2.75%and RBT 2.25%).Based on parity, significantly higher prevalence (MRT 2.93% and RBT 2.44%) of MRT and RBT were obtainedin parity 3-5in comparison to other parity group (p?0.01). It is, however, obvious that although the MRT is 1st-line screening tests for brucellosis in cows in some countries, their lack of specificity is of concern. Therefore, the requirement for other confirmatory tests that are more specific should be used for the diagnosis of the disease, especially in Bangladesh.DOI: http://dx.doi.org/10.3329/bjvm.v12i2.21280 Bangl. J. Vet. Med. (2014). 12 (2): 167-171 

scholarly journals Milk yield and composition in dairy cows with post-partum disorders

2021 ◽  
Vol 73 (3) ◽  
pp. 639-646
Author(s):  
C. Bondan ◽  
J.A. Folchini ◽  
L. Guimarães ◽  
M. Noro ◽  
R. Zanella ◽  
...  
Keyword(s):  
Milk Yield ◽  
Milk Fat ◽  
Body Condition Score ◽  
Post Partum ◽  
Dairy Farm ◽  
Rio Grande Do Sul ◽  
Clinical Disorders ◽  
Condition Score ◽  
The Impact ◽  
Score Evaluation

ABSTRACT This study aimed to determine the impact of different post-partum disorders on milk yield and composition. One hundred and fifteen Holstein cows from a commercial dairy farm located in the state of Rio Grande do Sul in southern Brazil were monitored up to 62 days post-partum. During this period, body condition score evaluation and animal clinical examination were conducted. Percentages of fat, protein, and lactose, as well as somatic cells score, were determined in milk samples. The AST activity and concentrations of NEFA, calcium, and BHBA, were analyzed in blood samples. The occurrence of clinical disorders was identified in 30 (26%) cows. Subclinical disorders were identified in 64 (56%) cows. Only 21 (18%) cows did not suffer any kind of disorder within the studied period. In this study, no significant differences were found in milk production, protein, and somatic cell count in clinical, subclinical, and healthy cows. Milk fat and the fat: protein quotient (F:P) were higher in cows with clinical disorders and the 6 to 21 days in milk, and lactose were lower in cows with clinical disorders and the 22 to 42 days in milk (P<0.05).

Oxytocin receptor concentrations, inositol phosphate turnover and prostaglandin release by endometrium from ewes induced to ovulate during the early post-partum period

1993 ◽  
Vol 136 (1) ◽  
pp. 17-NP ◽  
Author(s):  
J. M. Wallace ◽  
M. G. Thompson ◽  
R. P. Aitken ◽  
M. A. Cheyne
Keyword(s):  
Receptor Binding ◽  
Inositol Phosphate ◽  
Post Partum ◽  
Oxytocin Receptor ◽  
Receptor Expression ◽  
Luteal Function ◽  
Induction Of Ovulation ◽  
Release Device ◽  
Prostaglandin F

ABSTRACT Induction of ovulation early post partum in sheep is associated with a high incidence (30–40%) of premature luteolysis. The present study was designed to characterize oxytocin receptor levels, oxytocin-stimulated inositol phosphate (IP) turnover (second messenger) and oxytocin-stimulated prostaglandin F2α (PGF2α) release in the endometrium of post-partum ewes induced to ovulate 21 days after parturition and expected to exhibit a range of corpus luteal functions subsequently. Ovulation was induced on day 21 post partum using a controlled internal drug release device and pregnant mare serum gonadotrophin, and uterine tissues were collected on days 5, 10 or 15 of the cycle (n = 4/day). A further 12 ewes whose interval from previous parturition exceeded 150 days were similarly treated and acted as controls. Measurement of daily peripheral progesterone concentrations revealed that while all control ewes exhibited normal luteal function, abnormal luteal function was evident in two, two and one post-partum ewes studied on days 5, 10 and 15 of the cycle respectively. Oxytocin receptor binding was detected (by receptor-binding assay and in-vitro autoradiography) in the endometrium and myometrium of post-partum ewes at all three stages of the oestrous cycle but only at day 15 in control ewes. To determine IP turnover, 100 mg caruncular endometrium was incubated in duplicate for 2·5 h with 10 μCi [3H]inositol and treated with 0 or 2 μmol oxytocin/l for 30 min, then [3H]inositol mono-, bis- and trisphosphates were quantified. Oxytocin stimulated total IPs in all day-5 and day-15 post-partum ewes, in three of four day-10 ewes and in all day-15 control ewes. Basal endometrial PGF2α release measured in triplicate (100 mg/well) during a 2 h incubation was higher in post-partum versus control ewes on days 5 and 10 but not on day 15 of the cycle. Similarly, oxytocin stimulated PGF2α release to varying levels at all stages of the cycle in post-partum ewes but only on day 15 in control ewes. Irrespective of the treatment group endometrial oxytocin receptor number was significantly (P < 0·001) correlated with oxytocin-stimulated IP turnover and PGF2α release. Thus the induction of ovulation and the subsequent luteal phase in post-partum ewes is against a back ground of high oxytocin receptor expression and enhanced PGF2α release which in some ewes may contribute to abnormal luteal function. Journal of Endocrinology (1993) 136, 17–25

scholarly journals Expression of the Frizzled receptors and their co-receptors in calcified human aortic valves

2018 ◽  
Vol 96 (2) ◽  
pp. 208-214 ◽  
Author(s):  
Ateeque Siddique ◽  
Bin Yu ◽  
Kashif Khan ◽  
Ryan Buyting ◽  
Hamood Al-Kindi ◽  
...  
Keyword(s):  
Aortic Valve ◽  
Wnt Signaling ◽  
Quantitative Polymerase Chain Reaction ◽  
Disease Process ◽  
Receptor Expression ◽  
Cellular Mechanisms ◽  
Aortic Valves ◽  
Major Player ◽  
Frizzled Receptors ◽  
Human Aortic Valve

The cellular mechanisms that induce calcific aortic stenosis are yet to be unraveled. Wnt signaling is increasingly being considered as a major player in the disease process. However, the presence of Wnt Frizzled (Fzd) receptors and co-receptors LRP5 and 6 in normal and diseased human aortic valves remains to be elucidated. Immunohistochemistry and quantitative polymerase chain reaction were used to determine Fzd receptor expression in normal and calcified human aortic valve tissue, as well as human aortic valve interstitial cells (HAVICs) isolated from calcified and normal human aortic valves. There was significantly higher mRNA expression of 4 out of the 10 Fzd receptors in calcified aortic valve tissues and 8 out of the 10 in HAVICs, and both LRP5/6 co-receptors in calcified aortic valves (P < 0.05). These results were confirmed by immunohistochemistry, which revealed abundant increase in immunoreactivity for Fzd3, 7, and 8, mainly in areas of lipid core and calcified nodules of diseased aortic valves. The findings of abundant expression of Fzd and LRP5/6 receptors in diseased aortic valves suggests a potential role for both canonical and noncanonical Wnt signaling in the pathogenesis of human aortic valve calcification. Future investigations aimed at targeting these molecules may provide potential therapies for aortic valve stenosis.

scholarly journals Follicular structures of cows with cystic ovarian disease present altered expression of cytokines

Zygote ◽  
2019 ◽  
Vol 27 (05) ◽  
pp. 285-298 ◽  
Author(s):  
Antonela F. Stassi ◽  
Natalia C. Gareis ◽  
Belkis E. Marelli ◽  
Valentina Matiller ◽  
Cristian J.M. Leiva ◽  
...  
Keyword(s):  
Growth Factors ◽  
Inflammatory Cytokine ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Concentrations ◽  
Altered Expression ◽  
Ovarian Disease ◽  
Cellular Processes ◽  
Ovarian Cells ◽  
Cystic Ovarian Disease ◽  
Disease Present

SummaryOvulation is considered an inflammatory, cytokine-mediated event. Cytokines, which are recognized as growth factors with immunoregulatory properties, are involved in many cellular processes at the ovarian level. In this sense, cytokines affect fertility and are involved in the development of different ovarian disorders such as bovine cystic ovarian disease (COD). Because it has been previously demonstrated that ovarian cells represent both sources and targets of cytokines, the aim of this study was to examine the expression of several cytokines, including IL-1β, IL-1RA, IL-1RI, IL-1RII, IL-4 and IL-8, in ovarian follicular structures from cows with spontaneous COD. The protein expression of these cytokines was evaluated by immunohistochemistry. Additionally, IL-1β, IL-4 and IL-8 concentrations in follicular fluid (FF) and serum were determined by enzyme-linked immunosorbent assay (ELISA). In granulosa and theca cells, IL-1RI, IL-1RII, IL-1RA and IL-4 expression levels were higher in cystic follicles than in the control dominant follicles. The serum and FF concentrations of IL-1β and IL-4 showed no differences between groups, whereas IL-8 concentration was detected only in FF of cysts from cows with COD. The FF and serum concentrations of IL-1β and IL-8 showed no significant differences, whereas IL-4 concentration was higher in FF than in serum in both the control and COD groups. These results evidenced an altered expression of cytokines in ovaries of cows with COD that could contribute to the pathogenesis of this disease.

Fertility parameters of dairy cows with cystic ovarian disease after treatment with gonadotrophin-releasing hormone

2001 ◽  
Vol 149 (13) ◽  
pp. 383-386 ◽  
Author(s):  
G. A. Hooijer ◽  
M. A. A. J. van Oijen ◽  
K. Frankena ◽  
M. M. H. Valks
Keyword(s):  
Dairy Cows ◽  
Releasing Hormone ◽  
Ovarian Disease ◽  
Gonadotrophin Releasing Hormone ◽  
Cystic Ovarian Disease

Impaired insulin signaling pathway in ovarian follicles of cows with cystic ovarian disease

2015 ◽  
Vol 156 ◽  
pp. 64-74 ◽  
Author(s):  
G.J. Hein ◽  
C.G. Panzani ◽  
F.M. Rodríguez ◽  
N.R. Salvetti ◽  
P.U. Díaz ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Insulin Signaling ◽  
Ovarian Follicles ◽  
Insulin Signaling Pathway ◽  
Ovarian Disease ◽  
Impaired Insulin ◽  
Cystic Ovarian Disease

Alterations in key metabolic sensors involved in bovine cystic ovarian disease

2018 ◽  
Vol 120 ◽  
pp. 138-146 ◽  
Author(s):  
N.C. Gareis ◽  
E. Angeli ◽  
E. Huber ◽  
N.R. Salvetti ◽  
F.M. Rodríguez ◽  
...  
Keyword(s):  
Ovarian Disease ◽  
Cystic Ovarian Disease

Potential role of arteether on N-methyl-D-aspartate (NMDA) receptor expression in experimental cerebral malaria mice and extension of their survival

Parasitology ◽  
2019 ◽  
Vol 146 (12) ◽  
pp. 1571-1577 ◽  
Author(s):  
Sunil Kumar Singh ◽  
Hemlata Dwivedi ◽  
Sarika Gunjan ◽  
Bhavana Singh Chauhan ◽  
Swaroop Kumar Pandey ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Cerebral Malaria ◽  
Learning And Memory ◽  
Neurological Complication ◽  
Receptor Expression ◽  
Extreme Condition ◽  
Mammalian Brain ◽  
Cellular Mechanisms ◽  
Experimental Cerebral Malaria ◽  
Excitatory Neurotransmitter

AbstractCerebral malaria (CM) is the severe neurological complication causing acute non-traumatic encephalopathy in tropical countries. The mechanisms underlying the fatal cerebral complications are still not fully understood. Glutamate, a major excitatory neurotransmitter in the central nervous system of the mammalian brain, plays a key role in the development of neuronal cells, motor function, synaptic plasticity, learning and memory processes under normal physiological conditions. The subtypes of ionotropic glutamate receptor are N-methyl-D-aspartate receptors (NMDARs) which are involved in cellular mechanisms of learning and memory, synaptic plasticity and also mediate excitotoxic neuronal injury. In the present study, we found that glutamate level in synaptosomes, as well as expression of NMDAR, was elevated during the extreme condition of CM in C57BL6 mice. Arteether at 50 mg kg−1× 1, 25 mg kg−1× 2, days decreased the NMDAR expression and increased the overall survival of the experimental CM mice.

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