scholarly journals Endometrial and decidual stromal precursors show a different decidualization capacity

Reproduction ◽  
2020 ◽  
Vol 160 (1) ◽  
pp. 83-91 ◽  
Author(s):  
Maria Jose Ruiz Magaña ◽  
Jose Maria Puerta ◽  
Rocio Martínez-Aguilar ◽  
Tatiana Llorca ◽  
Osmany Blanco ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stromal Cells ◽  
Normal Development ◽  
Endometrial Stromal Cells ◽  
Pr Protein ◽  
Functional Differences ◽  
Subunit Expression ◽  
Stromal Cell Line ◽  
Induction Of Apoptosis ◽  
Pregnancy Hormones

Endometrial stromal cells (EnSCs) and decidual stromal cells (DSCs) originate from fibroblastic precursors located around the vessels of the human nonpregnant endometrium and the pregnant endometrium (decidua), respectively. Under the effect of ovarian or pregnancy hormones, these precursors differentiate (decidualize), changing their morphology and secreting factors that appear to be essential for the normal development of pregnancy. However, the different physiological context – that is, non-pregnancy vs pregnancy – of those precursors (preEnSCs, preDSCs) might affect their phenotype and functions. In the present study, we established preEnSC and preDSC lines and compared the antigen phenotype and responses to decidualization factors in these two types of stromal cell line. Analyses with flow cytometry showed that preEnSCs and preDSCs exhibited a similar antigen phenotype compatible with that of bone marrow mesenchymal stem/stromal cells. The response to decidualization in cultures with progesterone and cAMP was evaluated by analyzing changes in cell morphology by microscopy, prolactin and IL-15 secretion by enzyme immunoassay and the induction of apoptosis by flow cytometry. In all four analyses, preDSCs showed a significantly higher response than preEnSCs. The expression of progesterone receptor (PR), protein kinase A (PKA) and FOXO1 was studied with Western blotting. Both types of cells showed similar levels of PR and PKA, but the increase in PKA RI subunit expression in response to decidualization was again significantly greater in preDSCs. We conclude that preEnSCs and preDSCs are equivalent cells but differ in their ability to decidualize. Functional differences between them probably derive from factors in their different milieus.

scholarly journals Differentially-Expressed miRNAs in Ectopic Stromal Cells Contribute to Endometriosis Development: The Plausible Role of miR-139-5p and miR-375

2018 ◽  
Vol 19 (12) ◽  
pp. 3789 ◽  
Author(s):  
Kadri Rekker ◽  
Tõnis Tasa ◽  
Merli Saare ◽  
Külli Samuel ◽  
Ülle Kadastik ◽  
...  
Keyword(s):  
Mirna Expression ◽  
Stromal Cells ◽  
Molecular Mechanisms ◽  
High Throughput Sequencing ◽  
Disease Onset ◽  
Cell Types ◽  
Cell Type ◽  
Endometrial Stromal Cells ◽  
Stromal Cell Line ◽  
Cell Type Specific

microRNA (miRNA) expression level alterations between endometrial tissue and endometriotic lesions indicate their involvement in endometriosis pathogenesis. However, as both endometrium and endometriotic lesions consist of different cell types in various proportions, it is not clear which cells contribute to variability in miRNA levels and the overall knowledge about cell-type specific miRNA expression in ectopic cells is scarce. Therefore, we utilized fluorescence-activated cell sorting to isolate endometrial stromal cells from paired endometrial and endometrioma biopsies and combined it with high-throughput sequencing to determine miRNA alterations in endometriotic stroma. The analysis revealed 149 abnormally expressed miRNAs in endometriotic lesions, including extensive upregulation of miR-139-5p and downregulation of miR-375 compared to eutopic cells. miRNA transfection experiments in the endometrial stromal cell line ST-T1b showed that the overexpression of miR-139-5p resulted in the downregulation of homeobox A9 (HOXA9) and HOXA10 expression, whereas the endothelin 1 (EDN1) gene was regulated by miR-375. The results of this study provide further insights into the complex molecular mechanisms involved in endometriosis pathogenesis and demonstrate the necessity for cell-type-specific analysis of ectopic tissues to understand the interactions between different cell populations in disease onset and progression.

scholarly journals Development and Characterization of a Simian Virus 40 Immortalized Bovine Endometrial Stromal Cell Line

Endocrinology ◽  
2008 ◽  
Vol 150 (1) ◽  
pp. 485-491 ◽  
Author(s):  
Narayanan Krishnaswamy ◽  
Pierre Chapdelaine ◽  
Jacques P. Tremblay ◽  
Michel A. Fortier
Keyword(s):  
Epithelial Cells ◽  
Cell Line ◽  
Stromal Cells ◽  
Stromal Cell ◽  
Mapk Pathway ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Signal Transducer ◽  
Endometrial Stromal Cells ◽  
Stromal Cell Line

In ruminants, interferon-τ (IFNτ) is the maternal recognition signal inhibiting prostaglandin (PG) F2α production by endometrial epithelial cells and stimulating interferon-stimulated genes in the stroma. Stromal cells mediate the action of progesterone on epithelial cells during pregnancy. Our working hypothesis is that IFNτ acts as a molecular switch that turns on PGE2 production in endometrial stromal cells while suppressing PGF2α production from epithelial cells. In this report we document immortalization and functional characterization of a bovine stromal cell line from the caruncular region of the endometrium [caruncular stromal cell (CSC)]. Primary stromal cells were immortalized by nucleofection with simian virus 40 large T antigen and integrase. The resulting cell line, CSC, expresses stromal cell-specific vimentin, estrogen, and progesterone receptors, and is amenable for transient transfection. Basal and stimulated production of PGE2 is higher than PGF2α and associated with cyclooxygenase (COX) 2 expression. Phorbol myristate acetate (PMA) and IFNτ up-regulate COX2 and PG production in a dose-dependent manner. When added together, low concentrations of IFNτ inhibit PMA-induced COX2 expression; whereas this inhibition is lost at high concentrations. Expression of signal transducer and activator of transcription 1 is induced by IFNτ at all concentrations studied but is not modulated by PMA. Because expression of signal transducer and activator of transcription 1 does not exhibit the biphasic response to IFNτ, we investigated the p38 MAPK pathway using the selective inhibitor SB203580. Inhibition of the p38 MAPK pathway abolishes IFNτ action on PG production. In summary, CSC appears as a good stromal cell model for investigating the molecular mechanisms related to IFNτ action and PG production in the bovine. Interferon-t stimulates prostaglandin E2 production, and expression of cyclooxygenase 2 and signal transducer and activator of transcription 1 through two distinct signaling pathways in immortalized bovine endometrial stromal cells.

The Effect of Copper on Endometrial Receptivity and Induction of Apoptosis on Decidualized Human Endometrial Stromal Cells

2017 ◽  
Vol 25 (7) ◽  
pp. 985-999 ◽  
Author(s):  
Jose P. Carrascosa ◽  
David Cotán ◽  
Inmaculada Jurado ◽  
Manuel Oropesa-Ávila ◽  
Pascual Sánchez-Martín ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Endometrial Receptivity ◽  
Endometrial Stromal Cells ◽  
Induction Of Apoptosis ◽  
Effect Of Copper

Progesterone-independent induction of decidualization of endometrial stromal cells of patients with and without endometriosis

2015 ◽  
Vol 75 (07) ◽  
Author(s):  
J Thomczik ◽  
I Beyer ◽  
DM Baston-Büst ◽  
SJ Böddeker ◽  
G Wennemuth ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Endometrial Stromal Cells

Regulation of TIMP-1, -2 and -3 in human endometrial stromal cells during decidualization and under the influence of hCG

2006 ◽  
Vol 114 (S 1) ◽  
Author(s):  
S Krenzer ◽  
H Fluhr ◽  
M Deperschmidt ◽  
M Zwirner ◽  
D Wallwiener ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Endometrial Stromal Cells

ÜBER DIE BILDUNG VON ENDOMETRIALEN KÖRNCHENZELLEN BEI KASTRATINNEN UNTER HORMONEINFLUSS

1960 ◽  
Vol XXXIII (II) ◽  
pp. 261-276 ◽  
Author(s):  
G. Hellweg ◽  
J. Ferin ◽  
K. G. Ober
Keyword(s):  
Hormone Therapy ◽  
Stromal Cells ◽  
Sex Hormone ◽  
Substitution Therapy ◽  
Secretory Phase ◽  
Hormone Substitution ◽  
Endometrial Stromal Cells ◽  
Endometrial Stroma ◽  
K Cells

ABSTRACT 65 endometrial biopsies from castrated women who had received either natural or artificial sex hormone therapy were studied microscopically. Attention was paid to various histologic criteria, especially to the number of endometrial granulocytes (»K« cells, KZ). The following was obtained: The »K« cells are completely absent when no hormone substitution therapy is given. They were also lacking when the castrated patients were treated only with oestrogens, even if the dose given was ten-times that found in women during the reproductive ages. In contrast, the »K« cells developed from the endometrial stromal cells only under influence of progesterone, usually appearing first 8–10 days after the administration of the gestagen. The »K« cells were demonstrable in the number corresponding to a normal secretory phase only then, when the oestrogen-progesterone dosage ratio had induced a fully-developed secretory change, as measured by the usual histologic criteria. With an overdosage of oestrogen the »K« cells were either absent or were very sparse. Contrarily, an overdosage of progesterone had no influence on their number. The development of endometrial glands does not always entirely parallel that of the stroma in castrated patients following hormone therapy. A more exact indicator for the proper dose for the production of a secretory phase by hormone therapy seems to be the number of »K« cells in the endometrial stroma.

Inhibition of TMEM16A impedes embryo implantation and decidualization in mice

Reproduction ◽  
2018 ◽  
Author(s):  
Qianrong Qi ◽  
Yifan Yang ◽  
Kailin Wu ◽  
Qingzhen Xie
Keyword(s):  
Progesterone Receptor ◽  
Stromal Cells ◽  
Embryo Implantation ◽  
Mouse Uterus ◽  
Endometrial Stromal Cells ◽  
Uterine Endometrium ◽  
Molecule Inhibitor ◽  
Pathway Inhibition ◽  
And Function ◽  
Reproductive Processes

Recent studies revealed that TMEM16A is involved in several reproductive processes, including ovarian estrogen secretion and ovulation, sperm motility and acrosome reaction, fertilization, and myometrium contraction. However, little is known about the expression and function of TMEM16A in embryo implantation and decidualization. In this study, we focused on the expression and regulation of TMEM16A in mouse uterus during early pregnancy. We found that TMEM16A is up-regulated in uterine endometrium in response to embryo implantation and decidualization. Progesterone treatment could induce TMEM16A expression in endometrial stromal cells through progesterone receptor/c-Myc pathway, which is blocked by progesterone receptor antagonist or the inhibitor of c-Myc signaling pathway. Inhibition of TMEM16A by small molecule inhibitor (T16Ainh-A01) resulted in impaired embryo implantation and decidualization in mice. Treatment with either specific siRNA of Tmem16a or T16Ainh-A01 inhibited the decidualization and proliferation of mouse endometrial stromal cells. In conclusion, our results revealed that TMEM16A is involved in embryo implantation and decidualization in mice, compromised function of TMEM16A may lead to impaired embryo implantation and decidualization.

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