Emerging bio-sensing methods for mycotoxin analysis

2011 ◽  
pp. 359-384 ◽  
Author(s):  
I.E. Tothill
Keyword(s):  
Mycotoxin Analysis

scholarly journals Dried urine spots as sampling technique for multi-mycotoxin analysis in human urine

2021 ◽  
Author(s):  
Jessica Schmidt ◽  
Viktoria Lindemann ◽  
Monica Olsen ◽  
Benedikt Cramer ◽  
Hans-Ulrich Humpf
Keyword(s):  
Ochratoxin A ◽  
Human Urine ◽  
Sampling Strategy ◽  
Sampling Technique ◽  
Human Biomonitoring ◽  
Urine Samples ◽  
Isotope Dilution Analysis ◽  
Tenuazonic Acid ◽  
Stable Isotope Dilution Analysis ◽  
Mycotoxin Analysis

AbstractA simple and effective approach for HPLC-MS/MS based multi-mycotoxin analysis in human urine samples was developed by application of dried urine spots (DUS) as alternative on-site sampling strategy. The newly developed method enables the detection and quantitation of 14 relevant mycotoxins and mycotoxin metabolites, including citrinin (CIT), dihydrocitrinone (DH-CIT), deoxynivalenol (DON), fumonisin B1 (FB1), T-2 Toxin (T-2), HT-2 Toxin (HT-2), ochratoxin A (OTA), 2′R-ochratoxin A (2′R-OTA), ochratoxin α (OTα), tenuazonic acid and allo-tenuazonic acid (TeA + allo-TeA), zearalenone (ZEN), zearalanone (ZAN), α-zearalenol (α-ZEL), and β-zearalenol (β-ZEL). Besides the spotting procedure, sample preparation includes enzymatic cleavage of glucuronic acid conjugates and stable isotope dilution analysis. Method validation revealed low limits of detection in the range of pg/mL urine and excellent apparent recovery rates for most analytes. Stability investigation of DUS displayed no or only slight decrease of the analyte concentration over a period of 28 days at room temperature. The new method was applied to the analysis of a set of urine samples (n = 91) from a Swedish cohort. The four analytes, DH-CIT, DON, OTA, and TeA + allo-TeA, could be detected and quantified in amounts ranging from 0.06 to 0.97 ng/mL, 3.03 to 136 ng/mL, 0.013 to 0.434 ng/mL and from 0.36 to 47 ng/mL in 38.5%, 70.3%, 68.1%, and 94.5% of the samples, respectively. Additional analysis of these urine samples with an established dilute and shoot (DaS) approach displayed a high consistency of the results obtained with both methods. However, due to higher sensitivity, a larger number of positive samples were observed using the DUS method consequently providing a suitable approach for human biomonitoring of mycotoxin exposure.

scholarly journals Sampling of cereals: assessment of alternative protocols for mycotoxin analysis

TOS forum ◽  
2015 ◽  
Vol 2013 (1) ◽  
pp. 59
Author(s):  
Brigitte Mahaut ◽  
Guislaine Delor
Keyword(s):  
Mycotoxin Analysis

Developments in mycotoxin analysis: an update for 2012-2013

2014 ◽  
Vol 7 (1) ◽  
pp. 3-33 ◽  
Author(s):  
F. Berthiller ◽  
P.A. Burdaspal ◽  
C. Crews ◽  
M.H. Iha ◽  
R. Krska ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Sample Preparation ◽  
Ergot Alkaloids ◽  
Tandem Mass ◽  
Wide Range ◽  
Rapid Spread ◽  
Food And Feed ◽  
Mycotoxin Analysis ◽  
The Individual

This review highlights developments in mycotoxin analysis and sampling over a period between mid-2012 and mid-2013. It covers the major mycotoxins: aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxins, patulin, trichothecenes and zearalenone. A wide range of analytical methods for mycotoxin determination in food and feed were developed last year, in particular immunochemical methods and liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS)-based methods. After a section on sampling and sample preparation, due to the rapid spread and developments in the field of LC-MS/MS multimycotoxin methods, a separate section has been devoted to this area of research. It is followed by a section on mycotoxins in botanicals and spices, before continuing with the format of previous reviews in this series with dedicated sections on method developments for the individual mycotoxins.

Developments in mycotoxin analysis: an update for 2015-2016

2017 ◽  
Vol 10 (1) ◽  
pp. 5-29 ◽  
Author(s):  
F. Berthiller ◽  
C. Brera ◽  
M.H. Iha ◽  
R. Krska ◽  
V.M.T. Lattanzio ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Critical Review ◽  
Analytical Methods ◽  
Ergot Alkaloids ◽  
Sampling Strategies ◽  
Liquid Chromatography Mass Spectrometry ◽  
Recent Developments ◽  
Mycotoxin Analysis

This review summarises developments in the determination of mycotoxins over a period between mid-2015 and mid-2016. Analytical methods to determine aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxins, patulin, trichothecenes and zearalenone are covered in individual sections. Advances in proper sampling strategies are discussed in a dedicated section, as are methods used to analyse botanicals and spices and newly developed liquid chromatography mass spectrometry based multi-mycotoxin methods. This critical review aims to briefly discuss the most important recent developments and trends in mycotoxin determination as well as to address limitations of presented methodologies.

Multi-mycotoxin analysis of maize silage by LC-MS/MS

2010 ◽  
Vol 397 (2) ◽  
pp. 765-776 ◽  
Author(s):  
R. R. Rasmussen ◽  
I. M. L. D. Storm ◽  
P. H. Rasmussen ◽  
J. Smedsgaard ◽  
K. F. Nielsen
Keyword(s):  
Maize Silage ◽  
Mycotoxin Analysis

Developments in mycotoxin analysis: an update for 2010-2011

2012 ◽  
Vol 5 (1) ◽  
pp. 3-30 ◽  
Author(s):  
G. Shephard ◽  
F. Berthiller ◽  
P. Burdaspal ◽  
C. Crews ◽  
M. Jonker ◽  
...  
Keyword(s):  
Analytical Method ◽  
Analytical Methods ◽  
Method Development ◽  
Rapid Development ◽  
Ergot Alkaloids ◽  
Alternaria Toxins ◽  
Research Publications ◽  
Mycotoxin Analysis ◽  
The Subject ◽  
Conventional Methods

This review highlights developments in mycotoxin analysis and sampling over a period between mid-2010 and mid-2011. It covers the major mycotoxins: aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxin, patulin, trichothecenes, and zearalenone. Analytical methods for mycotoxins continue to be developed and published. Despite much interest in immunochemical methods and in the rapid development of LC-MS methodology, more conventional methods, sometimes linked to novel clean-up protocols, have also been the subject of research publications over the above period. Occurrence of mycotoxins falls outside the main focus of this review; however, where relevant to analytical method development, this has been mentioned.

Evaluation of rapid test kits for quantification of HT-2 and T-2 toxins in naturally contaminated oats

2013 ◽  
Vol 6 (1) ◽  
pp. 31-41 ◽  
Author(s):  
H.U. Aamot ◽  
I.S. Hofgaard ◽  
G. Brodal ◽  
O. Elen ◽  
B. Holen ◽  
...  
Keyword(s):  
Concentration Ratio ◽  
Rapid Test ◽  
Reference Method ◽  
Lateral Flow ◽  
Cross Reaction ◽  
Cross Reactions ◽  
Lateral Flow Device ◽  
Simultaneous Testing ◽  
Flow Device ◽  
Mycotoxin Analysis

The aim of this study was to evaluate the performance and usefulness of three rapid test kits for analysis of HT-2 and T-2 toxins (HT-2 and T-2), two of the most potent trichothecenes commonly found in European oats. Concentrations of these two toxins combined (HT-2+T-2) were analysed in naturally contaminated oat samples (n=68) using the following test kits: Ridascreen® FAST T-2 Toxin (‘Fast ELISA’), DRAFT Ridascreen® HT-2/T-2 (‘Standard ELISA’, not commercially available), and the lateral flow device ROSA® HT-2-T-2 (‘Rosa LFD’). Mycotoxin analysis by LC-MS/MS was used as a reference method. Rosa LFD offered the best reliability, achieving detection that was stable across toxin levels, whereas detection by both ELISA kits differed significantly among toxin levels (P<0.01). The kits were also evaluated regarding agreement with the reference method (measured as Cohen's kappa) at a HT-2+T-2 concentration of 1000 μg/kg in naturally contaminated oats. Agreement was greatest for Rosa LFD (89.2%), intermediate for Standard ELISA (66.8%), and lowest for Fast ELISA (62.2%). Rosa LFD showed cross-reaction of 100% with both T-2 and HT-2. For the ELISA kits, cross-reactions were 100% with T-2 but below 100% with HT-2. Therefore, to estimate the sum of HT-2 and T-2 in an oat sample, it was necessary to re-calculate the data from both ELISA kits according to the known cross-reaction of each kit with HT-2 and the concentration ratio of HT-2 to T-2 in Norwegian oats. Rosa LFD had the highest correlation with LC-MS/MS (R2=0.94), and the corresponding R2 values for Fast and Standard ELISA were 0.61 and 0.83, respectively. Rosa LFD was well suited for on-site detection. Standard ELISA allows simultaneous testing of several samples that are useful for centralised laboratories.

scholarly journals Biosensors and nanomaterials and their application for mycotoxin determination

2011 ◽  
Vol 4 (4) ◽  
pp. 361-374 ◽  
Author(s):  
I. Tothill
Keyword(s):  
Food Quality ◽  
Quantitative Methods ◽  
Scientific Literature ◽  
Cost Effective ◽  
Screening Tools ◽  
Field Analysis ◽  
Quality And Safety ◽  
Site Testing ◽  
Food Quality And Safety ◽  
Mycotoxin Analysis

Mycotoxin analysis and detection in food and drinks is vital for ensuring food quality and safety, eliminating and controlling the risk of consuming contaminated foods, and complying with the legislative limits set by food authorities worldwide. Most analysis of these toxins is still conducted using conventional methods; however, biosensor methods are currently being developed as screening tools for use in field analysis. Biosensors have demonstrated their ability to provide rapid, sensitive, robust and cost-effective quantitative methods for on-site testing. The development of biosensor devices for different mycotoxins has attracted much research interest in recent years with a range of devices being designed and reported in the scientific literature. However, with the advent of nanotechnology and its impact on the evolution of ultrasensitive devices, mycotoxin analysis is also benefiting from the advances taking place in applying nanomaterials in sensors development. This paper reviews the developments in the area of biosensors and their applications for mycotoxin analysis, as well as the development of micro/nanoarray transducers and nanoparticles and their use in the development of new rapid devices.

Sign in / Sign up

Export Citation Format

Share Document