Debittering of α-Casein Hydrolysates by a Fungal Peptidase

Kuhmilchallergie: Die Bedeutung von Registerstudien für die Allergieforschung

Karger Kompass Dermatologie ◽

10.1159/000503619 ◽

2019 ◽

pp. 200-202

Author(s):

Claudia Traidl-Hoffmann

Keyword(s):

Recovery Time ◽

Lactobacillus Rhamnosus ◽

Casein Hydrolysate ◽

Common Diagnosis ◽

Ige Mediated ◽

Casein Hydrolysates ◽

Type Of Delivery ◽

Delivery Type ◽

Gender Type

Introduction: Cowʼs milk allergy (CMA) is a common diagnosis in infants, requiring the exclusion of cowʼs milk until tolerance is recovered. In the present study, we aim to determine which factors are associated with the development of tolerance.Methods: Retrospective, observational study of subjects who underwent the same clinical follow-up methodology. We studied 245 cases of CMA (125 IgE-mediated and 120 non-IgE-mediated). The following variables were analysed: age at diagnosis, gender, type of delivery, type of feeding received, feeding during the first months of life, clinical features, and type of feed received as treatment: casein hydrolysates or casein hydrolysates with Lactobacillus rhamnosus GG (LGG).Results: Factors associated with earlier tolerance were non-IgE-mediated CMA (HR = 2.92; 95% CI: 2.20-3.88) and patients receiving casein hydrolysate with LGG (HR = 1.79; 95% CI: 1.33-2.42). Later tolerance was associated with caesarean delivery (HR = 0.78; 95% CI: 0.58-1.05) and breastfeeding for a period of at least 3 days (HR = 0.64; 95% CI: 0.44-0.93). The multivariate study shows that the type of formula (HR = 1.61; 95% CI: 1.19-2.18) and the type of CMA (HR = 2.82; 95% CI: 2.12-3.85) have an effect on the recovery time. Casein hydrolysates with LGG reduces the recovery time in IgE-mediated (HR = 1.88; 95% CI: 1.17-3.01) and non-IgE-mediated CMA (HR = 1.46; 95% CI: 0.98-2.17).Conclusion: Tolerance acquisition is faster in non-IgE-mediated CMA subjects and in those who received casein hydrolysate with LGG.

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Bioavailability of transglutaminase cross-linked sodium casein hydrolysates

Proceedings of The Nutrition Society ◽

10.1017/s0029665115002475 ◽

2015 ◽

Vol 74 (OCE4) ◽

Author(s):

M. Cermeño ◽

Y. O'Callaghan ◽

N. O'Brien ◽

R.J. FitzGerald

Keyword(s):

Casein Hydrolysates

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Effect of maltodextrin and gum Arabic carriers on the physicochemical properties and antioxidant activity of spray-dried casein hydrolysates

Food Science and Technology ◽

10.52547/fsct.17.107.131 ◽

2021 ◽

Vol 17 (107) ◽

pp. 131-145

Author(s):

zahra Akbarbaglu ◽

Asa kamali shojaei ◽

khashayar sarabandi ◽

◽

...

Keyword(s):

Antioxidant Activity ◽

Physicochemical Properties ◽

Gum Arabic ◽

Casein Hydrolysates ◽

Spray Dried

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CHEMICAL AND CLINICAL OBSERVATIONS DURING TREATMENT OF CHILDREN WITH PHENYLKETONURIA

PEDIATRICS ◽

10.1542/peds.21.6.929 ◽

1958 ◽

Vol 21 (6) ◽

pp. 929-940

Author(s):

Helen K. Berry ◽

Betty S. Sutherland ◽

George M. Guest ◽

Barbara Umbarger

Keyword(s):

Initial Period ◽

Caloric Intake ◽

The Past ◽

Clinical Observations ◽

Poor Nutritional Status ◽

Nutritional Disturbances ◽

Natural Foods ◽

Casein Hydrolysates ◽

To Receive ◽

Biochemical Abnormalities

Three children with phenylketonuria between the ages of 2½ and 4½ years have been treated with three different diets low in content of phenylalanine during the past 18 months. In Diets I and III restriction of phenylalanine was accomplished by use of casein hydrolysates from which phenylalanine had been removed and other amino acids added. In Diet II intake of phenylalanine was limited by restriction of total intake of protein in the diet. Diets II and III were commercial preparations. All diets were fed in semisolid form combined with natural foods. After an initial period of hospitalization for regulation of intake of phenylalanine, treatment was carried out at home. Except during the early months of treatment intake of phenylalanine was not severely restricted. Nutritional disturbances were encountered as a result of apparent amino acid deficiencies in Diet I. Inadequate caloric intake and low intake of protein also contributed to poor nutritional status at one stage in the treatment. Biochemical abnormalities characteristic of phenylketonuria were improved when phenylalanine was restricted in the diets of the three children. All three showed improvement in motor ability, increased awareness, lengthened attention span, decreased tenseness and irritability. No change in mental status on objective testing was demonstrated. The improvement in behavior motor control, and ease of handling was such that the parents were unwilling for the children to receive diets with normal content of phenylalanine. This reaction of the parents should be taken into consideration whenever administration of a diet low in content of phenylalanine is considered.

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PREPARATIONS OF BACTERIAL PROTEASES FOR THE PRODUCTION OF PROTEIN HYDROLYSATES WITHOUT BITTERNESS

BIOTECHNOLOGY: STATE OF THE ART AND PERSPECTIVES ◽

10.37747/2312-640x-2020-18-407-409 ◽

2020 ◽

pp. 407-409

Author(s):

A. Sereda ◽

I. Velikoretskaya ◽

D. Mineeva ◽

N. Tsurikova

Keyword(s):

Bacillus Subtilis ◽

Serine Proteases ◽

Culture Liquid ◽

Protein Hydrolysates ◽

Neutral Protease ◽

Enzyme Preparations ◽

Casein Hydrolysates

The effect of the neutral and serine proteases ratio in enzyme preparations (EP) on the bitterness of casein hydrolysates was studied. Bacillus subtilis 359 strain producing proteolytic complex with a serine to neutral protease ratio similar to Neutrase was selected from VNIIIPBT strains collection. The preparation from B. subtilis 359 culture liquid provides obtaining protein hydrolysates without bitterness.

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Comparative evaluation of feeding effects of A1 and A2 cow milk derived casein hydrolysates in diabetic model of rats

Journal of Functional Foods ◽

10.1016/j.jff.2020.104272 ◽

2020 ◽

Vol 75 ◽

pp. 104272

Author(s):

Neha Thakur ◽

Geeta Chauhan ◽

B.P. Mishra ◽

S.K. Mendiratta ◽

A.K. Pattanaik ◽

...

Keyword(s):

Comparative Evaluation ◽

Cow Milk ◽

Diabetic Model ◽

Feeding Effects ◽

Casein Hydrolysates

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Characterization of Proteolytic Activity of Artichoke (Cynara scolymus L.) Flower Extracts on Bovine Casein to Obtain Bioactive Peptides

Animals ◽

10.3390/ani10050914 ◽

2020 ◽

Vol 10 (5) ◽

pp. 914 ◽

Cited By ~ 1

Author(s):

Estefanía Bueno-Gavilá ◽

Adela Abellán ◽

María Soledad Bermejo ◽

Eva Salazar ◽

José María Cayuela ◽

...

Keyword(s):

Proteolytic Activity ◽

Radical Scavenging ◽

Enzyme Concentration ◽

Trolox Equivalent Antioxidant Capacity ◽

Antihypertensive Activity ◽

Hydrolysis Time ◽

Cynara Scolymus ◽

Trolox Equivalent ◽

Casein Hydrolysates

The aim of this work is to establish the most suitable proteolysis conditions to obtain bovine casein hydrolysates containing peptides with antioxidant and antihypertensive capacity. To this end, the proteolytic activity of Cynara scolymus L. flower extracts was characterized on whole bovine casein, evaluating the effect of several factors (pH, temperature, substrate concentration, enzyme concentration, and hydrolysis time). The optimal conditions to carry out the hydrolysis with the C. scolymus L. extract were as follows: pH 6.2, 50 °C, and 0.023 mg·mL−1 of extract-protein concentration. A Michaelis constant (Km) value of 5.66 mg·mL−1 and a maximum rate of reaction (Vmax) of 8.47 mUAbs∙min−1 were observed. The optimal hydrolysis time was 17 h. The casein hydrolysates obtained with these conditions contained peptides with antioxidant activity (1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity: 30.89%; Trolox equivalent antioxidant capacity (TEAC) against 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) free radical (ABTS●+): 4.43 mM Trolox equivalent·mg−1 peptide) and antihypertensive activity, showing 55.05% angiotensin-converting enzyme-I inhibition in vitro.

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