The Significance of N -Nitrosamines in the Histological Rebuilding of Gastric Mucosa in Patients with a Gastric Ulcer

Gastric ulcer (GU), a prevalent digestive disease, has a high incidence and is seriously harmful to human health. Finding a natural drug with a gastroprotective effect is needed. Ocotillol, the derivate of ocotillol-type saponins in the Panax genus, possesses good anti-inflammatory activity. The study aimed to investigate the gastroprotective effect of ocotillol on acetic acid-induced GU rats. The serum levels of endothelin-1 (ET-1) and nitric oxide (NO), the gastric mucosa levels of epidermal growth factor, superoxide dismutase and NO were assessed. Hematoxylin and eosin staining of gastric mucosa for pathological changes and immunohistochemical staining of ET-1, epidermal growth factor receptors and inducible nitric oxide synthase were evaluated. A UPLC-QTOF-MS-based serum metabolomics approach was applied to explore the latent mechanism. A total of 21 potential metabolites involved in 7 metabolic pathways were identified. The study helps us to understand the pathogenesis of GU and to provide a potential natural anti-ulcer agent.

Download Full-text

Synergy Effects of Three Plant Extracts on Protection of Gastric Mucosa

Natural Product Communications ◽

10.1177/1934578x1501001146 ◽

2015 ◽

Vol 10 (11) ◽

pp. 1934578X1501001 ◽

Cited By ~ 1

Author(s):

Caihui Wang ◽

Wen Su ◽

Xingli Su ◽

Guojun Ni ◽

Tao Liu ◽

...

Keyword(s):

Gastric Ulcer ◽

Gastric Mucosa ◽

Plant Extracts ◽

Synergistic Effects ◽

Damage Model ◽

Centella Asiatica ◽

Protection Effect ◽

Natural Plant ◽

Synergy Effects ◽

Amomum Villosum

The gastric mucosa protection effect of three natural plant extracts, Hericium erinaceus (HE), Centella asiatica (CA) and Amomum villosum (AV), were evaluated using the indomethacin damage model. Compared with a single extract, a combination of HE/CA/AV, especially with the ratios of 80:10:10, 45:45:10 and 45:10:45, showed significant synergistic effects for protection of the gastric mucosa with gastric ulcer inhibition rates of 97.8 ± 0.7%, 86.5 ± 2.8% and 86.1 ± 3.6%, respectively. Microscopic appearances of the gastric mucosa were carried out to help confirm the results.

Download Full-text

THE COMPARISON OF THE EFFECT BETWEEN ALGINATE-BASED RAFT-FORMING LIQUID AND ALGINATE LIQUID ON GASTROESOPHAGEAL REFLUX DISEASE AND GASTRIC ULCER IN RATS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i12.21096 ◽

2017 ◽

Vol 10 (12) ◽

pp. 105

Author(s):

Hakim Bangun ◽

Anayanti Arianto ◽

Ririn Astya ◽

Gontar A Siregar

Keyword(s):

Gastric Ulcer ◽

Gastroesophageal Reflux Disease ◽

Gastric Mucosa ◽

Gastroesophageal Reflux ◽

Negative Control ◽

Gastric Lesions ◽

Esophageal Erosion ◽

Group 3 ◽

Group 2 ◽

Group 1

Objective: The objective of the study was to compare the effect between alginate (Alg)-based raft-forming and Alg liquid on healing gastroesophageal reflux disease (GERD) and gastric ulcer in rats.Methods: Each of the 18 fasted rats was given 1 ml acidified pepsin. Then, rats were divided into three groups. Each group consisted of six rats. Group 1 (negative control) was orally given 1 ml distilled water, Group 2 was given 1 ml Alg-based raft-forming liquid, and Group 3 was given 1 ml Alg liquid. Then, the abdomen of rats was incised under anesthesia with ketamine, and then both their pylorus and the forestomach were ligated to form gastric reflux. After 4 hrs, all rats were killed with chloroform and their esophagus and stomach were examined macroscopically and microscopically (histopathology).Results: On macroscopic observation, all of the Group 1 rats (negative control) showed esophageal lesions and gastric lesions. Four rats of Group 2 (given Alg-based raft-forming) showed no esophageal lesion and two more rats showed a slight lesion, but all of the tested rats showed gastric lesions. All of the rats of the Group 3 (given Alg liquid) showed esophageal lesions, but no gastric lesion on four rats and slight lesion on two rats. Microscopic observations showed that all of the Group 1 rats (negative control) showed esophageal erosion and gastric mucosa lesions. Rats of the Group 2 (given Alg-based raft-forming) showed almost no esophageal erosion, but all of them showed erosion of gastric mucosa. Rats of the Group 3 (given Alg liquid) showed esophageal erosion in all tested rats, but almost no gastric mucosa lesion.Conclusion: Alg-base raft-forming liquid is more effective in healing of GERD than Alg liquid. However, Alg-liquid is more effective in healing of gastric ulcer than Alg-based raft-forming liquid.

Download Full-text

A STUDY ON THE RELATIONSHIP BETWEEN HELICOBACTER PYLORI AND TRACE ELEMENTS IN GASTRIC MUCOSA

International Journal of PIXE ◽

10.1142/s0129083506000812 ◽

2006 ◽

Vol 16 (01n02) ◽

pp. 55-68

Author(s):

T. FUKUDA ◽

S. SAKURAI ◽

M. KUDO

Keyword(s):

Helicobacter Pylori ◽

Trace Elements ◽

Gastric Ulcer ◽

Gastric Mucosa ◽

Gastric Ulcers ◽

Control Group ◽

Human Gastric Mucosa ◽

H2 Receptor Antagonist ◽

Zinc Levels ◽

The Relationship

H2 receptor antagonist (H2RA) and proton pump inhibitor(PPI) are often used to treat gastric ulcers, in addition to the zinc-L-carcino-complex which is commonly adopted for such therapies. However, there have been no previous reports detailing the levels and distribution of zinc in human gastric mucosa. The aim of our current study is to clarify the relationship between Helicobacter pylori infection and trace elements such as zinc in gastric mucosa using particle induced X-ray emission (PIXE). Forty gastric ulcer patients were chosen as subjects of this study and divided into two groups. One is a new ulcer patients group(twenty nine cases). And the other, eleven of these patients had been prescribed zinc-L-carcino-complex (polapreZinc®), at a dose of 150mg/day for periods ranging from 16 to 20 weeks. The zinc levels in the gastric ulcer group were found to be significantly less than those of the control group and the concentrations of zinc in gastric mucosa were observed to increase following the administration of treatments containing this element.

Download Full-text