Extraction, Characterization and Physico Chemical Properties of Chitin and Chitosan from Mud Crab Shell (Scylla Serrata)

2011 ◽  
Vol 3 (8) ◽  
pp. 44-46 ◽  
Author(s):  
A. Kiruba A. Kiruba ◽  
◽  
V. Uthayakumar V. Uthayakumar ◽  
S. Munirasu S. Munirasu ◽  
V. Ramasubramanian V. Ramasubramanian
Keyword(s):  
Chemical Properties ◽  
Scylla Serrata ◽  
Crab Shell ◽  
Mud Crab ◽  
Physico Chemical ◽  
Chitin And Chitosan

scholarly journals The Use of Different Deacetylation Temperature Toward Quality of Chitosan Mud Crab Shell (Scylla serrata)

2021 ◽  
Vol 934 (1) ◽  
pp. 012092
Author(s):  
R Karnila ◽  
S Loekman ◽  
S Humairah
Keyword(s):  
Moisture Content ◽  
Ash Content ◽  
Scylla Serrata ◽  
Crab Shell ◽  
Mud Crab ◽  
Degree Of Deacetylation ◽  
Randomized Design ◽  
Chitin And Chitosan ◽  
Two Stages

Abstract This research aims to determine the chemical composition (proximate) of chitosan and chitin, The best deacetylation temperature for obtaining chitosan, and the yield of chitosan and chitin from mud crab. This research was conducted in two stages, that is: 1) Preparation of mud crab flour and 2) Extraction and analysis of chitin and chitosan. The experimental design used for chitosan isolation was Completely Randomized Design (CRD). The process of deacetylation chitin becomes chitosan by using 50% NaOH with varying heating temperatures (120, 130, and 140°C). Parameters observed were yield, moisture content, ash content, and degrees of deacetylation. The result showed that the best chitosan was obtained by chitin deacetylation process into chitosan using a temperature of 130°C (KO2). Characteristics quality of the chitosan mud crab shell produced are KO1: yield 61.00%, moisture content 6.47%, ash content 17.18% (db), and degree of deacetylation 49.63%. KO2: yield 59.94%, moisture content 6.48%, ash content 14.85% (db) with degree of deacetylation 51.13%. KO3: yield 53,97, moisture content 6.54%, ash content 14.66% (db) and degree of deacetylation 52.63%. Characteristic quality of chitin included yield was 27.81%, moisture content 7.29%, ash 44.05%, and degree of deacetylation 33.09%.

scholarly journals Chitin, Chitosan, and Submicron-Sized Chitosan Particles Prepared from Scylla serrata Shells

2020 ◽  
Vol 2 (2) ◽  
pp. 139-149
Keyword(s):  
Molecular Weight ◽  
Sodium Tripolyphosphate ◽  
Average Molecular Weight ◽  
Value Added ◽  
Scylla Serrata ◽  
Mud Crab ◽  
Practical Applications ◽  
Degree Of Acetylation ◽  
Wide Range ◽  
Chitin And Chitosan

Extraction of chitin from mud crab (Scylla serrata) shells, involving demineralization and deproteinization, and deacetylation of the extracted chitin to form chitosan were investigated. The mud crab chitin and chitosan were obtained with a good yield (16.8% and 84.7% based on dried weight basis). The physicochemical properties, functional groups, molecular weight, and degree of acetylation of the chitin and chitosan were characterized. The surface morphology, the orientation arrangement of polysaccharide strands, and crystallinity of the chitin and chitosan prepared from the mud crab shells were investigated. SEM, FTIR, and XRD analyses demonstrated that the chitin consists of micron-sized fibrils, belonging to α from with the crystallinity of 60.1%. The chitosan has a viscosity-average molecular weight of 6.83 kDa with the degree of acetylation being 9.6% and the crystallinity of 73.8%. The chitosan was successfully fabricated into submicron-sized particles using top-down ionotropic gelation, microwave, and microemulsion methods, employing sodium tripolyphosphate, potassium persulfate, and glutaraldehyde as reagents, respectively. Overall, the results indicated that the preparation of chitin, chitosan, and submicron-sized chitosan particles from mud crab shells could open the opportunity for the value-added seafood waste to be utilized in a wide range of practical applications.

scholarly journals Chemical Proprieties of Biopolymers (Chitin/Chitosan) and Their Synergic Effects with Endophytic Bacillus Species: Unlimited Applications in Agriculture

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1117
Author(s):  
Rkhaila Amine ◽  
Chtouki Tarek ◽  
Erguig Hassane ◽  
El Haloui Noureddine ◽  
Ounine Khadija
Keyword(s):  
Chemical Properties ◽  
Bacillus Species ◽  
Agricultural Field ◽  
Bacterial Strains ◽  
Genus Bacillus ◽  
The Past ◽  
Physico Chemical ◽  
Potential Applications ◽  
Synthetic Pesticides ◽  
Chitin And Chitosan

Over the past decade, reckless usage of synthetic pesticides and fertilizers in agriculture has made the environment and human health progressively vulnerable. This setting leads to the pursuit of other environmentally friendly interventions. Amongst the suggested solutions, the use of chitin and chitosan came about, whether alone or in combination with endophytic bacterial strains. In the framework of this research, we reported an assortment of studies on the physico-chemical properties and potential applications in the agricultural field of two biopolymers extracted from shrimp shells (chitin and chitosan), in addition to their uses as biofertilizers and biostimulators in combination with bacterial strains of the genus Bacillus sp. (having biochemical and enzymatic properties).

scholarly journals Synthesis and Characterization of Chitosan from Prawn Shells and Study of Its Effects on Weight Loss of Myrica esculenta Fruits

2021 ◽  
Vol 33 (2) ◽  
pp. 299-306
Author(s):  
S. POKHREL ◽  
S SHAH ◽  
H.S. ADHIKARI
Keyword(s):  
Weight Loss ◽  
13C Nmr ◽  
Photoelectron Spectroscopy ◽  
Chemical Properties ◽  
Treatment Method ◽  
Chitosan Coating ◽  
Physico Chemical ◽  
Myrica Esculenta ◽  
Chitin And Chitosan

In this work, chitin and chitosan were obtained from prawn shell wastages by chemical treatment method. Structural characterization of chitin and chitosan by FTIR, X-ray photoelectron spectroscopy (XPS) and 13C NMR clearly showed the formation of chitosan from chitin. The physico-chemical properties of chitosan viz. molecular weight, moisture content, ash content and degree of deacetylation (DD) were analyzed. The optimum condition of deacetylation process to obtain chitosan from chitin was analyzed. The FTIR spectra showed the characteristic peaks corresponding to hydroxy, acetamido and amino functionalities of chitosan obtained from partial deacetylation of chitin and the solid state 13C NMR showed the formation of chitosan with characteristic peaks. XRD showed the shifting of crystallinity phases showing more crystallinity of chitin than chitosan. XPS spectrum of prepared chitin with the peaks corresponding to N, C and O binding energy was analogous to the standard. The effect of chitosan coating in extension of postharvest life of Kaphal (Myrica esculenta) fruits was investigated and chitosan coating was observed to have a potential to prolong storage life, control decay and weight loss.

Decoration of specific sites on freeze-fractured membranes

1978 ◽  
Vol 36 (2) ◽  
pp. 140-141
Author(s):  
H. Gross ◽  
H. Moor
Keyword(s):  
Pure Water ◽  
Freeze Fracture ◽  
Chemical Properties ◽  
Surface Forces ◽  
Sulfate Pentahydrate ◽  
Copper Sulfate Pentahydrate ◽  
Physico Chemical ◽  
Water Of Hydration ◽  
Small Container ◽  
Binding Forces

Fracturing under ultrahigh vacuum (UHV, p ≤ 10-9 Torr) produces membrane fracture faces devoid of contamination. Such clean surfaces are a prerequisite foe studies of interactions between condensing molecules is possible and surface forces are unequally distributed, the condensate will accumulate at places with high binding forces; crystallites will arise which may be useful a probes for surface sites with specific physico-chemical properties. Specific “decoration” with crystallites can be achieved nby exposing membrane fracture faces to water vopour. A device was developed which enables the production of pure water vapour and the controlled variation of its partial pressure in an UHV freeze-fracture apparatus (Fig.1a). Under vaccum (≤ 10-3 Torr), small container filled with copper-sulfate-pentahydrate is heated with a heating coil, with the temperature controlled by means of a thermocouple. The water of hydration thereby released enters a storage vessel.

Physico-Chemical Properties of Recombinant Desulphatohirudin

1990 ◽  
Vol 63 (03) ◽  
pp. 499-504 ◽  
Author(s):  
A Electricwala ◽  
L Irons ◽  
R Wait ◽  
R J G Carr ◽  
R J Ling ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gel Filtration ◽  
Chemical Properties ◽  
Alpha Helix ◽  
Apparent Molecular Weight ◽  
Correlation Spectroscopy ◽  
Nmr Studies ◽  
Recombinant Hirudin ◽  
Physico Chemical ◽  
Recombinant Molecule

SummaryPhysico-chemical properties of recombinant desulphatohirudin expressed in yeast (CIBA GEIGY code No. CGP 39393) were reinvestigated. As previously reported for natural hirudin, the recombinant molecule exhibited abnormal behaviour by gel filtration with an apparent molecular weight greater than that based on the primary structure. However, molecular weight estimation by SDS gel electrophoresis, FAB-mass spectrometry and Photon Correlation Spectroscopy were in agreement with the theoretical molecular weight, with little suggestion of dimer or aggregate formation. Circular dichroism studies of the recombinant molecule show similar spectra at different pH values but are markedly different from that reported by Konno et al. (13) for a natural hirudin-variant. Our CD studies indicate the presence of about 60% beta sheet and the absence of alpha helix in the secondary structure of recombinant hirudin, in agreement with the conformation determined by NMR studies (17)

A new shell disease in the mud crab Scylla serrata from Port Curtis, Queensland (Australia)

2000 ◽  
Vol 43 ◽  
pp. 233-239 ◽  
Author(s):  
LE Andersen ◽  
JH Norton ◽  
NH Levy
Keyword(s):  
Scylla Serrata ◽  
Mud Crab ◽  
Shell Disease
Sign in / Sign up

Export Citation Format

Share Document