Comparison of Two Phenotypic Methods, Prevalence and Antimicrobial Susceptibility Profile of Metallo-Β-Lactamases (M-Β-L) Producing Gram Negative Bacilli

2012 ◽  
Vol 2 (7) ◽  
pp. 312-315
Author(s):  
Dipti D Patel ◽  
◽  
Sanjay D Rathod ◽  
Pankti D Panchal ◽  
Bimal Chauhan ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Gram Negative ◽  
Antimicrobial Susceptibility Profile ◽  
Phenotypic Methods

scholarly journals Antimicrobial Sensitivity Profile of Bacteria Isolated in Babitonga Bay

2021 ◽  
Author(s):  
Maria Tereza Dalla Vecchia ◽  
Andréa Schneider ◽  
Paulo Henrique Condeixa de França ◽  
Regina Maria Miranda Gern
Keyword(s):  
Antimicrobial Susceptibility ◽  
Profile Analysis ◽  
Mangrove Sediment ◽  
Isolation And Identification ◽  
Gram Negative ◽  
Health Concerns ◽  
Microbial Identification ◽  
Antimicrobial Sensitivity ◽  
Antimicrobial Susceptibility Profile ◽  
Sensitivity Profile

Abstract Antimicrobial resistance is one of the main and growing public health concerns. However, the analysis of the expansion of this phenomenon in the environment is limited. Thus, the objective of this work was to determine the antimicrobial susceptibility profile of bacteria isolated from Baía Babitonga. Isolation and identification of microorganisms were carried out from the mangrove sediment sample, collected at two sampling points in Baía Babitonga. Microbial identification analyzes demonstrated the presence of Gram-negative microorganisms, for which the antimicrobial susceptibility profile analysis showed the presence of resistance to Aztreonam, Ciprofloxacin, Cefuroxim, Nitrofurantoin, Meropenem, Tetracyclin, Cefotaxime, and Ceftazidime. For the identified Gram-positive microorganisms, the presence of resistance to Ampicillin and Clindamycin was evidenced. The genotypic investigation showed that the resistances found were not caused by the researched genes (blaSHV, blaCTX-M, blaOXA-48-like, blaKPC, blaNDM-1, blaVIM e blaIMP).

scholarly journals Phenotypic methods for screening carbapenem-resistant Enterobacteriaceae and assessment of their antimicrobial susceptibility profile

2017 ◽  
Vol 50 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Danielly da Costa Silva ◽  
Roberta Filipini Rampelotto ◽  
Vinícius Victor Lorenzoni ◽  
Silvana Oliveira dos Santos ◽  
Juliana Damer ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Carbapenem Resistant ◽  
Antimicrobial Susceptibility Profile ◽  
Phenotypic Methods ◽  
Carbapenem Resistant Enterobacteriaceae

Rapid Identification and Antimicrobial Susceptibility of Gram Negative Bacteria Detected in Positive Blood Culture Bottles

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Nevine Nabil Kassem ◽  
Hala Mahmoud Hafez ◽  
Dalia H Abdelhamid ◽  
Ola Ali Mahmoud
Keyword(s):  
Blood Culture ◽  
Causative Agent ◽  
Positive Blood Culture ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Rapid Identification ◽  
Gram Negative ◽  
Antimicrobial Susceptibility Profile ◽  
Direct Primary

Abstract Background Blood stream infection (BSI) is one of the most serious situations in infectious disease .Accurate and timely identification of the causative agent and determination of its antimicrobial susceptibility profile are essential for guiding targeted and effective antimicrobial treatment. Current methods involve culturing of blood in a liquid medium and subsequently subculturing of signal positive bottles on solid media in order to obtain isolated colonies that can be further used in identification and susceptibility testing of the isolates. The standard method requires additional 48-72hours following the appearance of a positive signal in order to provide a reliable patient report. On the other hand, rapid identification of the causative agent and determination of its susceptibility profile by direct inoculation of the biochemical test media with the blood-broth mixtures from signal positive bottles and performing primary susceptibility testing might help reducing the time needed for provision of results compared to the standard isolated-colony based method and hence would help the rapid initiation of effective and targeted antimicrobial therapy and reduce the bacteremia-related morbidity and mortality. Objective The aim of the present study was to determine the accuracy and precision of the non-standard methods (direct identification and susceptibility testing using the blood/broth mixture) by comparing its results to those of the standard isolate-based identification and susceptibility testing methods. Material and method The study included 52 signal blood culture bottles yielding gram negative isolates. Bottles were selected amongst blood culture bottles submitted to the Main Microbiology laboratory, Ain Shams University hospital, for culture and antimicrobial susceptibility testing during the period between May 2018 and October 2018. a portion of the blood-broth mixture was aspirated from positive blood culture bottles, after being well mixed, and was subcultured onto agar media for the isolation of the causative agent and subsequently determination of its antimicrobial susceptibility profile was performed. Another part of the aspirated blood-broth mixture was diluted with sterile saline, its turbidity was adjusted against a 0.5McFarland standard and was used to inoculate directly the biochemical test media panel used for the identification of gram negative organisms as well as to perform direct (primary) antimicrobial susceptibility. Results The present study revealed there was 100% categorical agreement between the results of the direct biochemical inoculation method and those of the standard isolate-based inoculation method regarding the identification of the causative agent. The results of the direct biochemical identification method were also consistent giving rise to a 100% withinrun precision categorical agreement and a 100% between-run precision categorical agreement. The overall categorical agreement between the results of the standard isolate-based AST method and the results of the direct (primary susceptibility) AST method was 96.3% for the signal blood culture media. The major error rate was 0.5% whereas the minor error rate was 3.3% . Consistent results were also obtained for the AST done directly from the signal blood culture bottles since the between-run and within-run precision categorical agreement were 96.3% and 98.6%, respectively. Conclusion the overall performance of the AST done directly from positive blood culture bottles fulfilled the acceptable performance criteria specified in the Cumitech 31A so the direct method can be used for the earlier determination of AST and identification of Gram negative bacteria and thus to reduce the time for early initiation of appropriate antibiotic

scholarly journals Bacterial profile and antimicrobial susceptibility patterns of isolates among patients diagnosed with surgical site infection at a tertiary teaching hospital in Ethiopia: a prospective cohort study

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Gemedo Misha ◽  
Legese Chelkeba ◽  
Tsegaye Melaku
Keyword(s):  
Escherichia Coli ◽  
Cohort Study ◽  
Surgical Site Infection ◽  
Prospective Cohort Study ◽  
Antimicrobial Susceptibility ◽  
Prospective Cohort ◽  
Site Infection ◽  
Gram Negative ◽  
Tertiary Teaching ◽  
Susceptibility Patterns

Abstract Background Globally, surgical site infections are the most reported healthcare-associated infection and common surgical complication. In developing countries such as Ethiopia, there is a paucity of published reports on the microbiologic profile and resistance patterns of an isolates. Objective This study aimed at assessing the bacterial profile and antimicrobial susceptibility patterns of isolates among patients diagnosed with surgical site infection at Jimma Medical Center in Ethiopia. Methods A prospective cohort study was employed among adult patients who underwent either elective or emergency surgical procedures. All the eligible patients were followed for 30 days for the occurrence of surgical site infection (SSI). From those who developed SSI, infected wound specimens were collected and studied bacteriologically. Results Of 251 study participants, 126 (50.2%) of them were females. The mean ± SD age of the patients was 38 ± 16.30 years. The overall postoperative surgical site infection rate was 21.1% and of these 71.7% (38/53) were culture positive. On gram stain analysis, 78% of them were Gram-negative, 11.5% were Gram-positive and 10.5% were a mixture of two microbial growths. Escherichia coli accounted for (21.43%), followed by Pseudomonas aeruginosa (19.05%), Proteus species (spp.) 14.29%), Staphylococcus aureus (11.90%), Klebsiella species (11.90%), Citrobacter spp. (9.5%), streptococcal spp. (7.14%), Coagulase-negative S. aureus (CoNS) (2.38%) Conclusion Gram-negative bacteria were the most dominant isolates from surgical sites in the study area. Among the Gram-negative bacilli, Escherichia coli were the most common bacteria causing surgical site infection. As there is high antibiotic resistance observed in the current study, it is necessary for routine microbial analysis of samples and their antibiogram.

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