scholarly journals Using cell cross-section dimensions and digital image correlation to evaluate drying shrinkage and collapse in Eucalyptus nitens wood

BioResources ◽  
2020 ◽  
Vol 15 (3) ◽  
pp. 6149-6164
Author(s):  
Alan Dickson ◽  
Bernard Dawson

An approach combining maps of wood morphology and digital image correlation was developed to investigate the drying of Eucalyptus nitens wood. Maps of morphological features (vessel and ray distribution) and cell cross-section dimensions were acquired by confocal laser scanning microscopy. Shrinkage maps were generated using digital image correlation. There were statistically significant correlations between shrinkage/collapse and wood morphology at two levels. Firstly, there were positional relationships, with for example, both radial and tangential shrinkage increasing with increasing distance from vessel elements. Secondly, there were dimensional relationships, such as, cells with large perimeters (relative to their wall thickness) on average showing greater shrinkage. Generally, the positional relationships dominated the dimensional relationships. Detailed analysis over large areas allows for a fuller analysis of the interrelationship between wood morphology and drying shrinkage and collapse.

2016 ◽  
Vol 22 (2) ◽  
pp. 290-299 ◽  
Author(s):  
Martina Laňková ◽  
Jana Humpolíčková ◽  
Stanislav Vosolsobě ◽  
Zdeněk Cit ◽  
Jozef Lacek ◽  
...  

AbstractA number of fluorescence microscopy techniques are described to study dynamics of fluorescently labeled proteins, lipids, nucleic acids, and whole organelles. However, for studies of plant plasma membrane (PM) proteins, the number of these techniques is still limited because of the high complexity of processes that determine the dynamics of PM proteins and the existence of cell wall. Here, we report on the usage of raster image correlation spectroscopy (RICS) for studies of integral PM proteins in suspension-cultured tobacco cells and show its potential in comparison with the more widely used fluorescence recovery after photobleaching method. For RICS, a set of microscopy images is obtained by single-photon confocal laser scanning microscopy (CLSM). Fluorescence fluctuations are subsequently correlated between individual pixels and the information on protein mobility are extracted using a model that considers processes generating the fluctuations such as diffusion and chemical binding reactions. As we show here using an example of two integral PM transporters of the plant hormone auxin, RICS uncovered their distinct short-distance lateral mobility within the PM that is dependent on cytoskeleton and sterol composition of the PM. RICS, which is routinely accessible on modern CLSM instruments, thus represents a valuable approach for studies of dynamics of PM proteins in plants.


1997 ◽  
Vol 32 (1) ◽  
pp. 29-35
Author(s):  
E Umezaki ◽  
K Futase

Displacements on a cross-section of a heat-sealed area in laminated plastic film used for liquid package bags are measured by a digital image correlation. All the specimens used for obtaining the images necessary to measure the displacements are cut into the required shape from heat-sealed package bags. The images are recorded by an 8mm video camera via a microscope, and digitized by an image-processing device. Twenty-six images are used for obtaining the displacements between the no-load condition and failure of the specimens. As a result, a characteristic displacement is found in the heat-sealed area.


Author(s):  
Justin Kinda ◽  
Alexandra Bourdot ◽  
Laurent Charpin ◽  
Sylvie Michel-Ponnelle ◽  
Farid Benboudjema

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