scholarly journals ntra-species profiling of Cleome viscosa growing in Swat district (Pakistan)

2018 ◽  
Vol 26 (1) ◽  
pp. 52-55
Author(s):  
N. Muhammad ◽  
S. F. Wadood ◽  
W. Khan ◽  
N. Ali ◽  
M. Nisar
Keyword(s):  
Allelic Variation ◽  
Lower Surface ◽  
Protein Profiling ◽  
Yellow Colour ◽  
As Species ◽  
Sds Page ◽  
Specific Variation ◽  
Species Specific ◽  
High Level ◽  
Cleome Viscosa

Intra-specific genetic variation was studied in 28 genotypes of Cleome viscosa L. growing in Swat district, Khyber Pakhtunkhwa, Pakistan. It was found that genotypes showed the utmost allelic variation for leaf upper and lower surface with emerald green (75%), and yellow green (75%) respectively, other leaves lower and upper surfaces were (25%) green and yellow green (26%) respectively. The majority of C. viscosa genotypes were (50%) yellow flowers while others were with (29%) white yellow colour and (21%) dull yellow. Most of the seeds were with black (46%). The protein profiling was carried out on 12% gel electrophoresis; seven reproducible bands with molecular weight ranges from 180 to 10 KDa were detected in C. viscosa, the locus contribution toward genetic disagreement (LCTGD) of C. viscosa was 57%. Notably, L-3, L-4 L-5, was monomorphic in C. viscosa and was treated as species specific. L-1, L-2, L-7 were polymorphic. These bands showed 79%, 4%, 14% and 79% variation respectively. In the current investigation the intra-specific variation was observed limited and alone SDS-PAGE did not determine the high level of intra-specific variation; however, diverse germplasm were suggested to be acquired from various sources.

scholarly journals Genetic diversity in the natural population of threatened fruit- bearing plant species Monotheca buxifolia (Falc.) A.D.

2020 ◽  
Vol 10 (5) ◽  
pp. 44-48
Author(s):  
M. Noor ◽  
U. Nisar ◽  
K.U.K. Muhhamad
Keyword(s):  
Genetic Diversity ◽  
Plant Species ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Plant Conservation ◽  
Protein Profiling ◽  
As Species ◽  
Sds Page ◽  
Monotheca Buxifolia ◽  
Species Specific

Monotheca buxifolia is an ethnomedicinally and economically important threatened fruit bearing plant species in Malakand Division Pakistan. The genetic diversity among the 92 various genotypes of Monotheca buxifolia was carried out using sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE) method. A considerable amount of inter districts genetic diversity (66.70%) was observed among the genotypes of M. buxifolia. Protein profiling was conducted on 12% gel electrophoresis. A total of 6 protein bands were observed in M. buxifolia genotypes. SDS-PAGE practice is a convenient scheme for the examination of both genetic diversity and relationship. Particularly, L-4 and L-5 were monomorphic in the inter districts Monotheca buxifolia genotypes and was recognized as species specific. The remaining other loci were polymorphic. In this investigation, the high inter and intra- districts specific diversity was observed demonstrating SDS-PAGE is an authoritative procedure for categorizing the genetically diverse germplasms in M. buxifolia. The findings from this study could be useful in the identification and selection of suitable M. buxifolia genotypes for future conservation programmes. Today, there is still a need to examine the genetic diversity and protect genetic resources, in particular wild species, for possible benefits in plant conservation programmes. To the best of our knowledge, this is the first ever report that addresses genetic variability in M. buxifolia.

Rapid Seafood Species Identification Using Chip-Based Capillary Electrophoresis and Protein Pattern Matching

2017 ◽  
Vol 100 (5) ◽  
pp. 1500-1510 ◽  
Author(s):  
Calvin C Walker ◽  
Cheryl L Lassitter ◽  
Shannara N Lynn ◽  
Courtney B Ford ◽  
Kevin R Rademacher ◽  
...  
Keyword(s):  
Protein Extraction ◽  
Protein Pattern ◽  
Screening Method ◽  
Fish Muscle ◽  
Protein Profiling ◽  
Rapid Screening ◽  
Important Species ◽  
Commercially Important ◽  
Species Specific ◽  
High Level

Abstract Authenticity is crucial to the seafood industry, as substitution and mislabeling have important economic, environmental, and food safety consequences. Toaddress this problem, protein profiling and softwarealgorithm techniques were developed to classify fishmuscle samples by species. The method uses water-based protein extraction, chip-based microfluidic electrophoresis (Agilent 2100 Bioanalyzer) for the analysis of high abundance fish muscle proteins, and a novel data analysis method for species-specific proteinpattern recognition. The method's performance in distinguishing commercially important fish from commonly reported substitutions was evaluated using sensitivity, specificity, and accuracy determinations with all three performance measures at >98% for commonsubstitutions. This study demonstrates that uncookedseafood products of commercially important species of catfish, snapper, and grouper can be rapidly distinguished from commonly substituted species with a high level of confidence. A tiered testing approach toseafood species verification by sequentially applying a rapid screening method and DNA testing is proposed to more effectively ensure accurate product labeling.

scholarly journals Evaluation of biochemical and serological methods to identify and clustering yeast cells of oral Candida species by CHROMagar test, SDS-PAGE and ELISA

2004 ◽  
Vol 64 (2) ◽  
pp. 317-326 ◽  
Author(s):  
J. A. de O. Rodrigues ◽  
J. F. Höfling ◽  
F. C. A. Tavares ◽  
K. M. R. Duarte ◽  
R. B. Gonçalves ◽  
...  
Keyword(s):  
Candida Species ◽  
Profile Analysis ◽  
Protein Profile ◽  
Negative Control ◽  
Yeast Cells ◽  
Sds Page ◽  
Differential Medium ◽  
Oral Yeasts ◽  
Species Specific ◽  
High Level

The purpose of this work was to evaluate biochemical and serological methods to characterize and identify Candida species from the oral cavity. The strains used were five Candida species previously identified: C. albicans, C. guilliermondii, C. parapsilosis, C. krusei, C. tropicalis, and Kluyveromyces marxianus, as a negative control. The analyses were conducted through the SDS-PAGE associated with statistical analysis using software, chromogenic medium, and CHROMagar Candida (CA), as a differential medium for the isolation and presumptive identification of clinically important yeasts and an enzyme-linked immunoabsorbent assay (ELISA), using antisera produced against antigens from two C. albicans strains. This method enabled the screening of the three Candida species: C. albicans, C. tropicalis, and C. Krusei, with 100% of specificity. The ELISA using purified immunoglobulin G showed a high level of cross-reaction against protein extracts of Candida species. The SDS-PAGE method allowed the clustering of species-specific isolates using the Simple Matching coefficient, S SM = 1.0. The protein profile analysis by SDS-PAGE increases what is known about the taxonomic relationships among oral yeasts. This methodology showed good reproducibility and allows collection of useful information for numerical analysis on information relevant to clinical application, and epidemiological and systematical studies.

scholarly journals Accurate Identification of Closely Related Mycobacterium tuberculosis Complex Species by High Resolution Tandem Mass Spectrometry

2021 ◽  
Vol 11 ◽  
Author(s):  
Amol O. Bajaj ◽  
Suraj Saraswat ◽  
Juha E. A. Knuuttila ◽  
Joanna Freeke ◽  
J. Benjamin Stielow ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mycobacterium Tuberculosis ◽  
Protein Profiling ◽  
Resolving Power ◽  
Accurate Identification ◽  
Complex Species ◽  
Characterization Methods ◽  
As Species ◽  
Mass Spectrometric Approach ◽  
Species Specific

Rapid and accurate differentiation of Mycobacterium tuberculosis complex (MTBC) species from other mycobacterium is essential for appropriate therapeutic management, timely intervention for infection control and initiation of appropriate health care measures. However, routine clinical characterization methods for Mycobacterium tuberculosis (Mtb) species remain both, time consuming and labor intensive. In the present study, an innovative liquid Chromatography-Mass Spectrometry method for the identification of clinically most relevant Mycobacterium tuberculosis complex species is tested using a model set of mycobacterium strains. The methodology is based on protein profiling of Mycobacterium tuberculosis complex isolates, which are used as markers of differentiation. To test the resolving power, speed, and accuracy of the method, four ATCC type strains and 37 recent clinical isolates of closely related species were analyzed using this new approach. Using different deconvolution algorithms, we detected hundreds of individual protein masses, with a subpopulation of these functioning as species-specific markers. This assay identified 216, 260, 222, and 201 proteoforms for M. tuberculosis ATCC 27294™, M. microti ATCC 19422™, M. africanum ATCC 25420™, and M. bovis ATCC 19210™ respectively. All clinical strains were identified to the correct species with a mean of 95% accuracy. Our study successfully demonstrates applicability of this novel mass spectrometric approach to identify clinically relevant Mycobacterium tuberculosis complex species that are very closely related and difficult to differentiate with currently existing methods. Here, we present the first proof-of-principle study employing a fast mass spectrometry-based method to identify the clinically most prevalent species within the Mycobacterium tuberculosis species complex.

scholarly journals Love at first flight: Wing interference patterns are species-specific and sexually dimorphic in blowflies (Diptera: Calliphoridae)

2020 ◽  
Author(s):  
Nathan J. Butterworth ◽  
Thomas E. White ◽  
Phillip G. Byrne ◽  
James F. Wallman
Keyword(s):  
Sexually Dimorphic ◽  
Quantitative Evidence ◽  
Sexual Dimorphisms ◽  
Australian Species ◽  
Insect Wings ◽  
As Species ◽  
Tentative Model ◽  
Specific Variation ◽  
Species Specific ◽  
Mating Cues

AbstractWing interference patterns (WIPs) are stable structural colours displayed on insect wings which are only visible at specific viewing geometries and against certain backgrounds. These patterns are widespread among flies and wasps, and growing evidence suggests that they may function as species- and sex-specific mating cues in a range of taxa. As such, it is expected that WIPs should differ between species and show clear sexual dimorphisms. However, the true extent to which WIPs vary between species, sexes, and individuals is currently unclear, as previous studies have only taken a qualitative approach, without considering how WIPs might be perceived by the insect. Here, we perform the first quantitative analysis of inter- and intra-specific variation in WIPs across seven Australian species of the blowfly genus Chrysomya. Using multispectral digital imaging and a tentative model of blowfly colour vision, we provide quantitative evidence that WIPs are species-specific, highlight that the extent of divergence is greater in males than in females, and demonstrate sexual dimorphisms in several species. These data provide evidence that WIPs have diversified substantially in blowflies and suggests that sexual selection may have played a role in this process.

Comparison of restriction fragment length polymorphisms of ribosomal DNA between Diphyllobothrium nihonkaiense and D. latum

1992 ◽  
Vol 66 (4) ◽  
pp. 261-266 ◽  
Author(s):  
T. Matsuura ◽  
G. Bylund ◽  
K. Sugane
Keyword(s):  
Restriction Fragment ◽  
Ribosomal Dna ◽  
Fragment Length ◽  
Restriction Fragment Length Polymorphisms ◽  
As Species ◽  
Length Polymorphisms ◽  
Specific Variation ◽  
Species Specific ◽  
Diphyllobothrium Latum ◽  
Restriction Fragment Length

ABSTRACTRestriction fragment length polymorphisms (RFLPs) of ribosomal DNA (rDNA) were compared between Diphyllobothrium latum and D. nihonkaiense using seven kinds of restriction endonucleases. No intra-specific variation in restriction fragment profiles was shown within both species of Diphyllobothrium. Digestion of the genomic DNA with three endonucleases, Smal, Hinfl and Hhal, provided one or two different bands between two species, although the hybridization patterns generated with the others, Hindlll, Xbal, Styl and Haelll, were the same in both. RFLPs in the digested profiles with Smal, Hinfl and Hhal could be used as species-specific markers even if only fragments of strobilae with morphological similarity were available. Other cestodes, Spirometra erinacei and Taenia saginata, used as controls showed quite different restriction fragment patterns with all the enzymes used.

scholarly journals Flight-call as species-specific signal in South American parrots and the effect of species relatedness in call similarity

2017 ◽  
Vol 25 (3) ◽  
pp. 143-151 ◽  
Author(s):  
Carlos B. de Araújo ◽  
Paulo A. M. Marques ◽  
Jacques M. E. Vielliard
Keyword(s):  
South American ◽  
Specific Signal ◽  
As Species ◽  
Species Specific

scholarly journals Correction to: Ultraviolet absorbance of Sphagnum magellanicum, S. fallax and S. fuscum extracts with seasonal and species‑specific variation

2021 ◽  
Vol 20 (4) ◽  
pp. 597-598
Author(s):  
J. Tienaho ◽  
N. Silvan ◽  
R. Muilu-Mäkelä ◽  
P. Kilpeläinen ◽  
E. Poikulainen ◽  
...  
Keyword(s):  
Ultraviolet Absorbance ◽  
Specific Variation ◽  
Species Specific ◽  
Sphagnum Magellanicum

scholarly journals Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Marwa Hamouda
Keyword(s):  
Genetic Diversity ◽  
Electrophoretic Pattern ◽  
Issr Markers ◽  
Silybum Marianum ◽  
Inter Simple Sequence Repeats ◽  
Sds Page ◽  
Dna Pattern ◽  
Pharmaceutical Properties ◽  
High Level ◽  
Rapd And Issr

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.

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