scholarly journals Частота виявлення мікоплазм урогенітального тракту жінок у м. Дніпропетровськ

2014 ◽  
Vol 5 (1) ◽  
pp. 45-48
Author(s):  
K. V. Bubalo ◽  
L. P. Golodok ◽  
A. I. Vinnikov
Keyword(s):  
Pathogen Detection ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Culture Method ◽  
Test System ◽  
Urogenital Tract ◽  
Laboratory Diagnostics ◽  
Different Ages ◽  
Inflammatory Processes ◽  
Genital Mycoplasmas

The frequency of urogenital mycoplasmas detection in women of different ages was studied in culture with the help of DUO test-system in order to determine their etiological significance in the development of inflammatory processes of women urogenital tract. We identified the researched cultures Mycoplasma hominis, Ureaplasma urealyticum in the diagnostic titer >104 TEM/ml indicating severe contamination by microorganisms, and in the titer <103 TEM/ml, the carrier state of the identified microorganisms. Of 120 studied isolates of women urogenital tract there have been identified 113 strains of genital mycoplasmas, among which 63%  – U. urealyticum, 32% – M. hominis, 3% – microbial association of U. urealyticum – M. hominis. According to the study of frequency of detection of urogenital mycoplasma using DUO test-system culture method, it was found that the most frequently observed ones were U. urealyticum in 75 women (63%) of all individuals, M. hominis in 38 women (32%) in different diagnostic titers (>104 TEM/ml, <103 TEM/ml) in 4 women (3%) U. urealyticum – M. hominis was observed in microbial associations and mycoplasma were not found in 3 women (2%) of all surveyed patients. U. urealyticum and M. hominis in the diagnostic titer of >104 TEM/ml was observed in 55 women (46%) and 20 women (17%), respectively, and the titer of <103 CFU/ml U. urealyticum was observed in 20 women (17%), and M. hominis in 18 women (15%). Analysis of genital mycoplasmas distribution among women of different ages has shown that there was the certain correlation between the patient age and frequency of genital mycoplasmas detection: the highest detection rate was observed in women age of 24–29. The dominant pathogen of urogenital tract inflammatory processes in women in 24–29 age group is U. urealyticum. The comparison of DUO test-system and PCR data has shown that DUO test-system in culture allowed more sensitive quantitave characterization of mycoplasmas, however, for the more effective laboratory diagnostics it was necessary to use complex methods to increase the probability of pathogen detection. Incidence of mycoplasmas in women with the presence of inflammation was higher than in women having the inflammation in the genital tract. In this case, potential symptom-free carriers exist for the development of inflammation of urogenital tract of women. Scientists have proved that mycoplasma could cause vulvovaginitis, urethritis, paraurethritis, bartholinitis, adnexitis, salpingitis, endometritis, and ovaritis. 

Mycoplasma and Chlamydia in Adenoids and Tonsils of Children Undergoing Adenoidectomy or Tonsillectomy

1994 ◽  
Vol 103 (2) ◽  
pp. 135-138 ◽  
Author(s):  
David Huminer ◽  
Rudi Levy ◽  
Silvio Pitlik ◽  
Zmira Samra
Keyword(s):  
Chlamydia Trachomatis ◽  
Chlamydial Infection ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Pathogenetic Role ◽  
Mycoplasma Spp ◽  
Genital Mycoplasmas

The prevalence of mycoplasmal and chlamydial infection was assessed in 83 children undergoing adenoidectomy, tonsillectomy, or both procedures for recurrent adenotonsillitis or obstructive symptoms. Throat smears (surface specimens) and minced adenoids and tonsils (core specimens) were cultured for Mycoplasma spp and for Chlamydia spp. Isolation rates in adenoidal specimens were as follows: Mycoplasma hominis, surface 7.1%, core 2.9%; and Ureaplasma urealyticum, surface 1.4%, core 2.9%. Mycoplasma hominis was also found in tonsillar specimens: surface 14.3%, core 20%. Chlamydia trachomatis was isolated only from a single core adenoidal specimen. The rate of mycoplasma isolation was significantly higher in children with recurrent adenotonsillitis (34.5%) than in those with obstructive symptoms (3.7%). Our findings document colonization of genital mycoplasmas in adenoids and tonsils of children with recurrent adenotonsillitis. Further studies are needed to evaluate the possible pathogenetic role of these microorganisms in adenotonsillar infection.

scholarly journals UROGENITAL MYCOPLASMA ROLE IN REPRODUCTIVE PATHOLOGIES

2017 ◽  
Vol 1 (65) ◽  
pp. 71-76 ◽  
Author(s):  
Ольга Островская ◽  
Olga Ostrovskaya ◽  
Марина Власова ◽  
Marina Vlasova ◽  
Ольга Кожарская ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Preterm Delivery ◽  
Detection Rate ◽  
Ureaplasma Urealyticum ◽  
Recurrent Miscarriage ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Tissue Samples ◽  
Mycoplasma Detection ◽  
Genital Mycoplasmas

The objective of the research was to study genital mycoplasma contribution to the development of reproductive disorders. Mycoplasma detection rate in genital smears of pregnant women of Khabarovsk was studied by a polymerase chain reaction (PCR) method. Ureaplasma (urealyticum+parvum) was detected in 56.3% of cases; Mycoplasma hominis in 7.5% of cases, Mycoplasma genitalium in 0% of cases. Genital mycoplasmas were detected in chorionic and fetal tissue samples in early-term spontaneous miscarriage cases, suction biopsies in women with recurrent miscarriage, tissue samples in elective abortions, placental samples of women whose pregnancy ended in preterm delivery, and placental samples of women who gave birth to children at term. Ureaplasma (urealyticum+parvum) DNA was detected in 11.9%, 18.8%, 1.4%, 32.6%, and 25.0% of cases, respectively; Mycoplasma hominis DNA in 7.4%, 12.5%, 2.6%, 2.3%, and 0% of cases, respectively; Mycoplasma genitalium DNA in 5.2%, 6.3%, 1.4%, 2.3%, 0%, respectively. Genital mycoplasmas were detected in autopsy material of children who had died in neonatal and postnatal periods. Relationship between mycoplasma concentration in vaginal flora of pregnant women and preterm delivery was identified using real-time PCR. Mycoplasma detection rate in reproductive pathologies was found to be significantly higher than in control groups. The results of the study favor the view of etiopathogenetic role of mycoplasmas in development of complications and outcomes of pregnancy.

scholarly journals Genital Mycoplasmas in Placental Infections

1994 ◽  
Vol 1 (6) ◽  
pp. 275-281 ◽  
Author(s):  
Andreas Stein ◽  
Léon Boubli ◽  
Bernard Blanc ◽  
Didier Raoult
Keyword(s):  
Genital Tract ◽  
Ureaplasma Urealyticum ◽  
Fatal Outcome ◽  
Anaerobic Bacteria ◽  
Mycoplasma Hominis ◽  
Lower Genital Tract ◽  
Bacteria And Fungi ◽  
True Infection ◽  
Aerobic And Anaerobic ◽  
Genital Mycoplasmas

Objective:The involvement of the genital mycoplasmasUreaplasma urealyticumandMycoplasma hominisin complications of pregnancy has remained controversial especially because these microorganisms are frequent colonizers of the lower genital tract. Recovery of bacteria from the placenta appears to be the sole technique to represent a true infection and not vaginal contamination. Therefore, we investigated the presence of genital mycoplasmas, aerobic and anaerobic bacteria, and fungi in human placentas and evaluated their association with morbidity and mortality of pregnancy.Methods:We cultured placentas from 82 women with complicated pregnancies. One hundred placentas from women with uncomplicated pregnancies were evaluated as controls. When possible, placentas were examined histologically for presence of chorioamnionitis.Results:Microorganisms were recovered from 52% of the placentas of complicated pregnancies andU. urealyticumwas the microorganism isolated most frequently from the placenta. A significant association between positive mycoplasma culture of the placenta and complication of pregnancy was found, and chorioamnionitis was positively related to isolation of mycoplasmas.Conclusions:These data suggest that genital mycoplasmas are able to infect the human placenta where they can cause chorioamnionitis. This infection of the placenta by genital mycoplasmas is related to preterm birth and fatal outcome of pregnancy.

Association between genital mycoplasmas, acute chorioamnionitis and fetal pneumonia in spontaneous abortions

2018 ◽  
Vol 46 (5) ◽  
pp. 503-508 ◽  
Author(s):  
Maria Agnese Latino ◽  
Giovanni Botta ◽  
Claudia Badino ◽  
Daniela De Maria ◽  
Annalisa Petrozziello ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Ureaplasma Urealyticum ◽  
Strong Association ◽  
Mycoplasma Hominis ◽  
Fungal Culture ◽  
Spontaneous Abortions ◽  
Ureaplasma Parvum ◽  
Microbial Invasion ◽  
Genital Mycoplasmas

Abstract Aim: Ureaplasma parvum, Ureaplasma urealyticum and Mycoplasma hominis are also known as genital mycoplasmas. Acute chorioamnionitis is an inflammation of the placenta associated with miscarriage. We retrospectively evaluated a possible association between genital mycoplasmas detection, acute chorioamnionitis and fetal pneumonia from second and third trimester spontaneous abortions. Methods: One hundred and thirty placenta and fetal lung samples were evaluated for histological examination. The placenta samples, along with corresponding fetal tracheo-bronchial aspirates, also underwent bacterial and fungal culture and real-time polymerase chain reaction (PCR) assay for the detection of genital mycoplasmas. Results: Acute chorioamnionitis and pneumonia were diagnosed in 80/130 (61.5%) and 22/130 (16.9%) samples, respectively. Among samples positive for acute chorioamnionitis, the proportion of samples positive by real-time PCR and/or culture, was significantly higher than that of negative controls [54/80 (67.5%) vs. 26/80 (32.5%); P<0.001]. Ureaplasma parvum detection was significantly associated with acute chorioamnionitis compared to controls [9/11 (81.8%) vs. 2/11 (18.2%); P=0.019], as well as U. urealyticum [6/7 (85.7%) vs. 1/7 (14.3%); P=0.039]. Among tracheo-bronchial aspirates from abortions with pneumonia, the proportion of real-time PCR and/or culture positive samples was significantly higher than that of controls [13/22 (59.1%) vs. 9/22 (40.9%); P=0.029]. Conclusions: A strong association was found between acute histologic chorioamnionitis and microbial invasion with U. parvum and/or U. urealyticum.

scholarly journals Laboratory diagnostics of urogenital mycoplasma colonization and infection

2021 ◽  
Vol 51 (4) ◽  
pp. 68-72
Author(s):  
L. N. Novikova ◽  
A. E. Taraskina
Keyword(s):  
Cultural Studies ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
High Specificity ◽  
Laboratory Diagnostics ◽  
Specificity And Sensitivity

The paper presents data on the possibilities of determining urogenital mycoplasmas (Mycoplasma hominis and Ureaplasma urealyticum) using some variants of culture and PCR methods. The scheme of cultural studies in full is given, with the help of which high specificity and sensitivity indicators are achieved. The proposed research algorithm for detecting Mycoplasma hominis and Ureaplasma urealyticum, based on the use of culture and PCR methods as complementary.

Management of sexually transmissible infections in the era of multiplexed molecular diagnostics: a primary care survey

Sexual Health ◽  
2018 ◽  
Vol 15 (4) ◽  
pp. 298 ◽  
Author(s):  
Tal Brosh-Nissimov ◽  
Ron Kedem ◽  
Nimrod Ophir ◽  
Omri Shental ◽  
Nathan Keller ◽  
...  
Keyword(s):  
Military Service ◽  
Trichomonas Vaginalis ◽  
Pathogen Detection ◽  
Ureaplasma Urealyticum ◽  
Real Life ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Risk Groups ◽  
Screening Programs ◽  
Sexually Transmissible Infections

Background Data regarding sexually transmissible infections (STI) often originate from STI clinics, screening programs or laboratory-based studies, thus are biased for specific risk groups or lack clinical details. This real-life observational study presents sample data of most young adult Israeli population by exploiting the centralised diagnostic and documentation platforms resulting from a mandatory military service at the age of 18 years for both genders. Methods: All STI diagnoses of Israeli Defence Forces soldiers during a 6-month period were reviewed. Patients with Chlamydia trachomatis (CT), Mycoplasma genitalium (MG), Neisseria gonorrhoeae (NG) and Trichomonas vaginalis (TV) (major-STI) and Ureaplasma urealyticum (UU), Ureaplasma parvum (UP) and Mycoplasma hominis (MH) (equivocal STI) were compared with STI-negative controls. Results: Sexually transmissible infection positivity rates (n = 2816) were as follows: CT 6.6%; MG 1.9%; NG 0.7%; TV 0.5%; UU 15.7%; UP 28.2%; and MH 6.2%. The CT+MG coinfection rate was 4.1%, yet CT+NG coinfections were rare (≈0.5%). More than half of the patients with ureaplasmas and/or MH were treated; 40% of them were recommended partner treatment. Most antibiotics were prescribed to patients with equivocal infections. Classic STI symptoms in males were linked to major-STI and UU, while females were asymptomatic or presented non-specific symptoms. Conclusions: The judicious use of antibiotics in the era of antimicrobial resistance necessitates re-evaluating the significance of equivocal pathogen detection and reporting (MH, UU, UP). Likewise, universal empiric treatment for NG should be reconsidered in light of its low rates in non-high-risk groups. Conversely, a high MG rate, a pathogen with potential resistance to common STI protocols, requires evaluation of guidelines adequacy.

scholarly journals Preterm Premature Rupture of the Membranes and Genital Mycoplasmas

2009 ◽  
Vol 52 (3) ◽  
pp. 117-120 ◽  
Author(s):  
Marian Kacerovský ◽  
Michal Pavlovský ◽  
Jindřich Tošner
Keyword(s):  
Pregnant Women ◽  
Bacterial Culture ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Normal Pregnancy ◽  
Control Group ◽  
Vaginal Smears ◽  
Premature Rupture ◽  
Preterm Premature Rupture ◽  
Genital Mycoplasmas

Objective: The purpose of this study was to evaluate the prevalence of cervical colonization by genital mycoplasmas in patients with preterm premature rupture of the membranes (PPROM). Method: We studied 225 women between 24 and 36 weeks of gestation with PPROM. Cervical swabs were obtained for genital mycoplasmas and standard vaginal smears of bacterial culture were performed at the time of patients’ admission. In the control group were 225 women with a normal pregnancy. Results: Ureaplasma urealyticum was detected in 68 % (152/225) and Mycoplasma hominis was detected in 28 % (63/225) of the patients with PPROM between 24 and 36 weeks of gestation and. In the control group Ureaplasma urealyticum was found in 17 % (38/225) and Mycoplasma hominis in 15 % (35/225) pregnant women. Conclusion: Our results provide evidence of an association between cervical colonization with genital mycoplasmas and preterm premature rupture of the membranes.

scholarly journals Epidemiology, Prevalence and Antimicrobial Susceptibility of Sexually Transmitted Mycoplasma hominis and Ureaplasma urealyticum Infections in Dschang, West Cameroon

2020 ◽  
pp. 19-29
Author(s):  
Thomas Djifack Tadongfack ◽  
Irina Lydia Sudeu Nitcheu ◽  
François Roger Nguepy Keubo ◽  
Henri Donald Mutarambirwa ◽  
Romeo Hervis Tedjieu ◽  
...  
Keyword(s):  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Cross Sectional Study ◽  
Resistance Rate ◽  
Health Policies ◽  
Cross Sectional ◽  
Sexually Transmitted ◽  
Sensitivity Rate ◽  
Susceptibility Profiles ◽  
Genital Mycoplasmas

Background: Genital Mycoplasmas play a key etiological role in several urogenital diseases among both Men and Women. Just to mention the few, they are often responsible of non-gonococcal urethritis, spontaneous abortion, preterm birth, low birth weight, infertility and perinatal mortality. Aim: The study aimed to assess the prevalence of genital infections with Mycoplasma hominis, Ureaplasma urealyticum and their co-infection, as well as the susceptibility profiles to antibiotics commonly prescribed in Dschang, Cameroon. Study Design: This was a 5-years retrospective cross-sectional study (including data from January 1, 2015 to December 31, 2019) conducted at Saint Vincent de Paul Hospital in Dschang, Cameroon. Methods: Data of 338 participants received at the Gyneco-obstetric and Internal Medicine units, properly recorded, were collected from the Hospital Microbiology Laboratory records. Results: The overall prevalence to genital Mycoplasmas was 57.4%, with 44.4% attributed to Ureaplasma urealyticum, 5.9% to Mycoplasma hominis and 7.1% to the Co-infection with the two bacteria isolates. Josamycin was the most sensitive antibiotic against Ureaplasma urealyticum (85.3%) and the co-infection (91.7%). Mycoplasma hominis isolates were more susceptible to three fluoroquinolones and tetracyclines with an equal sensitivity rate of 80.0%. Emphasis is on the increasing resistance of co-infection towards Macrolides (83.3%). Summarily, Josamycin was the antibiotic to which genital Mycoplasmas showed the lowest resistance rate (6.2%) while the highest (62.9%) was attributed to Acetylspyramycin. Conclusion: The implementation of health policies in Cameroon should optimize on mechanisms of diagnosis, proper treatment and monitoring the antibiotic resistance of commonly isolated genital Mycoplasmas, regarding their impact on reproductive health.

Detection of the sexually transmissible genital mycoplasmas by polymerase chain reaction in women

Sexual Health ◽  
2011 ◽  
Vol 8 (3) ◽  
pp. 445 ◽  
Author(s):  
Vessela V. Ouzounova-Raykova ◽  
Rumyana Markovska ◽  
Gergana Mizgova ◽  
Ivan G. Mitov
Keyword(s):  
Polymerase Chain Reaction ◽  
Ureaplasma Urealyticum ◽  
Mycoplasma Hominis ◽  
Mycoplasma Genitalium ◽  
Chain Reaction ◽  
Clinical Disorders ◽  
Polymerase Chain ◽  
Asymptomatic Women ◽  
Genital Mycoplasmas

Background The role of Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium in the development of clinical disorders is still unclear. The aim of this study was to determine their prevalence in symptomatic and asymptomatic women. Methods: A total of 348 women were examined by applying polymerase chain reaction (PCR) methods. Results: The registered prevalence was as follows: U. urealyticum 14.66%; M. hominis 3.16%; and M. genitalium 0.29%. Co-infection was established in 11 swabs. Conclusions: This is the first study in Bulgaria for the detection of mycoplasmas by PCR. Our results demonstrate similar or lower values in comparison with other researchers and further investigations are needed.

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