scholarly journals New finding of green algae with potential for algal biotechnology, Chlorococcum oleofaciens and its molecular investigation

2017 ◽  
Vol 8 (4) ◽  
pp. 532-539 ◽  
Author(s):  
Y. I. Maltsev ◽  
T. V. Konovalenko
Keyword(s):  
Light Microscopy ◽  
Molecular Genetic ◽  
Taxonomic Status ◽  
Forest Litter ◽  
Base Change ◽  
Compensatory Base Change ◽  
Algal Biotechnology ◽  
The Status ◽  
Biochemical Research ◽  
Different Strains

The practice of soil algology shows that algae from the order Chlamydomonadales are among the most poorly studied and difficult to identify due to the high heterogeneity of their morphology and ultrastructure. Only the involvement of molecular genetic methods usually makes it possible to determine their taxonomic status with high accuracy. At the same time, in the algae flora of Ukraine there are more than 250 species from the order Chlamydomonadales, the status of which in most cases is established exclusively on the basis of light microscopy. This work is devoted to the study of the biotechnologically promising green alga Chlorococcum oleofaciens, taking into account the modern understanding of its taxonomic status. Two new strains of this species, separated from samples of forest litter and oak forest soil (the Samara Forest, Dnipropetrovsk region), are described. The strains were studied at the morphological level by using light microscopy methods, as well as using molecular genetic methods based on the studies of the nucleotide genes sequences of the 18S ribosomal DNA (rDNA) and chloroplast rbcL genes, the topology of secondary structures of internal transcribed spacer 2 (ITS2). The obtained results helped to confirm the presence of C. oleofaciens in the algae flora of Ukraine. Also, the authors of the article discuss the differences in the secondary structure of ITS2 in different strains of C. oleofaciens associated with the presence of compensatory base change (CBC), hemi-CBC in helices I and II, as well as deletions in helix IV and providing a basis for the hypothesis of the existence of cryptic species within C. oleofaciens. The obtained data can be used at the stage of preliminary selection of biochemical research objects. 

Molecular and morphological characterization ofEchinococcusin cervids from North America

Parasitology ◽  
2005 ◽  
Vol 132 (3) ◽  
pp. 439-447 ◽  
Author(s):  
R. C. A. THOMPSON ◽  
A. C. BOXELL ◽  
B. J. RALSTON ◽  
C. C. CONSTANTINE ◽  
R. P. HOBBS ◽  
...  
Keyword(s):  
North America ◽  
Molecular Genetic ◽  
Taxonomic Status ◽  
Morphological Characterization ◽  
Final Decision ◽  
Molecular Tools ◽  
Intermediate Hosts ◽  
The Status ◽  
Genetic Techniques

Many issues concerning the taxonomy ofEchinococcushave been resolved in recent years with the application of molecular tools. However, the status ofEchinococcusmaintained in transmission cycles involving cervid intermediate hosts remains to be determined. The recent characterization of the parasite from cervids in Finland has highlighted the paucity of data available, particularly that from North America. In this study, we have characterized a large number ofEchinococcusisolates from cervids from Western Canada on the basis of morphology and molecular genetic techniques. Our results support earlier studies suggesting thatEchinococcusof cervid origin is phenotypically and genetically distinct toEchinococcusmaintained in domestic host assemblages, and also confirms thatEchinococcusof cervid origin does not constitute a genetically homogeneous group. However, our data do not support the existence of 2 distinct genotypes (strains/subspecies) with separate geographical distributions. Our data appear to support the existence of only 1 species in cervids, but additional isolates from cervids and wolves in other endemic regions should be characterized before a final decision is made on the taxonomic status ofEchinococcusin cervids.

scholarly journals Comparison of fatty acid profiles of different strains of diatom Cyclotella meneghiniana Kützing from the salt lake Tahilt-Nuur (Mongolia)

2021 ◽  
Vol 20 (1) ◽  
pp. 246-248
Author(s):  
Z. V. Krivova ◽  
Y. I. Maltsev ◽  
M. S. Kulikovskiy
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Salt Lake ◽  
Molecular Genetic ◽  
Taxonomic Status ◽  
Omega 3 ◽  
Genetic Studies ◽  
Cyclotella Meneghiniana ◽  
Different Strains ◽  
Long Chain

Diatoms are a widespread group of microorganisms, many of which are able to accumulate significantamounts of long-chain and polyunsaturated fatty acids. 5 strains of Cyclotella meneghiniana were isolated during the studyof diatom flora in the salt lakes of Mongolia. The taxonomic status was confirmed by morphological and molecular genetic studies, and the features of the fatty acid composition were also established. We found that Cyclotella meneghinianastrains are producers of long-chain polyunsaturated omega-3 fatty acids and are of interest for further biotechnologicalapplications.

On three unpublished lectotype designations and on the status and positions of Kreizbergius simo-group taxa (Lepidoptera, Papilionidae)

2018 ◽  
Vol 69 (2) ◽  
pp. 98-106
Author(s):  
Stanislav K. Korb
Keyword(s):  
Taxonomic Status ◽  
New Synonyms ◽  
The Status

The taxonomic status and positions of taxa described within the genus Kreizbergius Korshunov, 1990 (the simo-group sensu Ackery, 1975) are rearranged. Lectotypes of the following taxa are designated: Parnassius simo var. simonius Staudinger, 1889, Parnassius simo var. simulator Staudinger, 1889, Parnassius gylippos Fruhstorfer, 1903, Parnassius simo confusa Bang-Haas, 1927. It is shown that K. anrdeji (Eisner, 1930) is a subspecies of K. simonius and has the oldest available name K. simonius kozlovi Avinov, 1913. New synonyms within K. simo and K. simonius are established.

Cuticular structures in the copulatory apparatus of Planorbis planorbis (Linnaeus, 1758) (Gastropoda: Pulmonata: Planorbidae)

2009 ◽  
Vol 18 (1) ◽  
pp. 11-16
Author(s):  
E.V. Soldatenko ◽  
A.A. Petrov
Keyword(s):  
Light Microscopy ◽  
Confocal Laser Scanning Microscopy ◽  
Laser Scanning ◽  
Taxonomic Status ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Copulatory Apparatus ◽  
The Family ◽  
Cuticular Structures

The morphology of the copulatory apparatus and associated cuticular structures in Planorbis planorbis was studied by light microscopy, SEM, TEM and confocal laser scanning microscopy. The significance of these cuticular structures for the taxonomic status of the species and for the systematics of the family Planorbidae in general is discussed.

scholarly journals Molecular Diversity of the Syndinean Genus Euduboscquella Based on Single-Cell PCR Analysis

2011 ◽  
Vol 78 (2) ◽  
pp. 334-345 ◽  
Author(s):  
Tsvetan R. Bachvaroff ◽  
Sunju Kim ◽  
Laure Guillou ◽  
Charles F. Delwiche ◽  
D. Wayne Coats
Keyword(s):  
Large Subunit ◽  
Small Subunit ◽  
Base Change ◽  
Pcr Analysis ◽  
Ssu Rrna ◽  
Compensatory Base Change ◽  
Content Type ◽  
Single Cell Pcr ◽  
Group I ◽  
Lsu Rrna

ABSTRACTThe genusEuduboscquellais one of a few described genera within the syndinean dinoflagellates, an enigmatic lineage with abundant diversity in marine environmental clone libraries based on small subunit (SSU) rRNA. The region composed of the SSU through to the partial large subunit (LSU) rRNA was determined from 40 individual tintinnid ciliate loricae infected withEuduboscquellasampled from eight surface water sites in the Northern Hemisphere, producing seven distinct SSU sequences. The corresponding host SSU rRNA region was also amplified from eight host species. The SSU tree ofEuduboscquellaand syndinean group I sequences from environmental clones had seven well-supported clades and one poorly supported clade across data sets from 57 to 692 total sequences. The genusEuduboscquellaconsistently formed a supported monophyletic clade within a single subclade of group I sequences. For most parasites with identical SSU sequences, the more variable internal transcribed spacer (ITS) to LSU rRNA regions were polymorphic at 3 to 10 sites. However, inE. cachonithere was variation between ITS to LSU copies at up to 20 sites within an individual, while in a parasite ofTintinnopsisspp., variation between different individuals ranged up to 19 polymorphic sites. However, applying the compensatory base change model to the ITS2 sequences suggested no compensatory changes within or between individuals with the same SSU sequence, while one to four compensatory changes between individuals with similar but not identical SSU sequences were found. Comparisons between host and parasite phylogenies do not suggest a simple pattern of host or parasite specificity.

A new slipper lobster of the genus Petrarctus Holthuis, 2002 (Crustacea, Decapoda, Scyllaridae) from Southwest coast of India

Zootaxa ◽  
2017 ◽  
Vol 4329 (5) ◽  
pp. 477 ◽  
Author(s):  
CHIEN-HUI YANG ◽  
APPUKUTTANNAIR BIJU KUMAR ◽  
TIN-YAM CHAN
Keyword(s):  
New Species ◽  
Deep Sea ◽  
Genetic Information ◽  
Anterior Part ◽  
Molecular Genetic ◽  
Taxonomic Status ◽  
Molecular Genetic Analysis ◽  
Andaman Sea ◽  
Abdominal Somite ◽  
A New Species

A new species of slipper lobster of the genus Petrarctus Holthuis, 2002 was discovered from southwestern India during a survey of deep sea crustaceans. The new species closely resembles P. veliger Holthuis, 2002 from the Andaman Sea and western Pacific but differs mainly in the color marking on abdominal somite I, having a relatively lower cardiac tooth but with better developed tubercles on the abdomen, as well as a differently shaped anterior part of the thoracic sternum. Molecular genetic analysis also confirms the distinct taxonomic status of the new species. To fix the identity of the type species of the genus, a neotype of P. rugosus (H. Milne Edwards, 1837) was selected from a recently collected Indian specimen with color and genetic information. 

Pseudodactylaria brevis sp. nov. from Thailand confirms the status of Pseudodactylariaceae

Phytotaxa ◽  
2018 ◽  
Vol 369 (4) ◽  
pp. 241 ◽  
Author(s):  
CHUAN-GEN LIN ◽  
ERIC H. C. MCKENZIE ◽  
DARBHE J. BHAT ◽  
JIAN-KUI LIU ◽  
KEVIN D. HYDE ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Sequence Data ◽  
Taxonomic Status ◽  
Its Sequence ◽  
Morphological Comparison ◽  
Phylogenetic Placement ◽  
Data Analyses ◽  
The Status ◽  
A New Species

A new species, Pseudodactylaria brevis, is described, illustrated and compared with other Pseudodactylaria and Dactylaria-like taxa. Evidence for the new species is provided by morphological comparison and sequence data analyses. Pseudodactylaria brevis can be distinguished from other Pseudodactylaria and Dactylaria-like species by its short hyaline conidiophores and fusiform, 1-septate hyaline conidia. Phylogenetic analysis of LSU and ITS sequence data was carried out to determine the phylogenetic placement of the species and confirm the taxonomic status of Pseudodactylariaceae.

Species relationships in the dasyurid marsupial genus Pseudantechinus (Marsupialia : Dasyuridae): a re-examination of the taxonomic status of Pseudantechinus roryi

2017 ◽  
Vol 65 (4) ◽  
pp. 240 ◽  
Author(s):  
L. S. Umbrello ◽  
P. A. Woolley ◽  
M. Westerman
Keyword(s):  
Dna Sequences ◽  
Isolation By Distance ◽  
Phylogenetic Analyses ◽  
Mitochondrial Gene ◽  
Taxonomic Status ◽  
Museum Collections ◽  
Species Relationships ◽  
Type Specimens ◽  
Network Analyses ◽  
The Status

The status of Pseudantechinus roryi relative to its congeners has been determined from DNA sequences obtained from both nuclear and mitochondrial gene loci. Although all other recognised species of Pseudantechinus form reciprocally monophyletic lineages in phylogenetic analyses, individuals identified in museum collections as Ps. roryi (including type specimens) were indistinguishable from those identified as Ps. macdonnellensis. Ps. roryi is thus considered to be a synonym of Ps. macdonnellensis. Neighbour-joining network analyses failed to reveal any clear biogeographic differences between populations of Ps. macdonnellensis other than some evidence of isolation by distance.

The status of the races of the graceful crag lizard, Pseudocordylus capensis, from South Africa

1992 ◽  
Vol 13 (2) ◽  
pp. 109-119 ◽  
Author(s):  
Y.M. Herselman ◽  
J.H. Van Wyk ◽  
P. le F.N. Mouton
Keyword(s):  
South Africa ◽  
Geographical Variation ◽  
Taxonomic Status ◽  
Morphological Characters ◽  
Distribution Range ◽  
The Status ◽  
Formal Taxonomy ◽  
New Distribution

AbstractTo evaluate the taxonomic status of the races of the graceful crag lizard, Pseudocordylus capensis, geographical variation in 44 external morphological characters was determined, using 97 specimens from 31 localities. Two different epiphenotypes predominate in the northern and southwestern parts of the distribution range. These epiphenotypes are, however, connected by a continuum of variation. It is therefore suggested that the recognition of races be abandoned in the formal taxonomy. Several new distribution records for the species are provided.

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