scholarly journals ВПЛИВ ІМУНОМОДУЛЯТОРІВ ПРИРОДНОГО ПОХОДЖЕННЯ НА КОНЦЕНТРАЦІЮ ПРОТЕЇНОВИХ ФРАКЦІЙ ТА РІВЕНЬ КОРТИЗОЛУ У ПЛАЗМІ КРОВІ КРОЛИКІВ ЗА УМОВ СТРЕСУ

2015 ◽  
Vol 5 (02) ◽  
pp. 93
Author(s):  
S. S. Grabovskyi ◽  
O. S. Grabovska
Keyword(s):  
Blood Plasma ◽  
Plasma Protein ◽  
Alcohol Solution ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Protein Fractions ◽  
Natural Origin ◽  
Blood Plasma Protein ◽  
Active Substances

<p>The results of determination of protein fractions, cortisol content in blood of rabbits, which further added to the feed of natural origin biologically active substances are presented in the article. As an antistressors and immunomodulators in pre-slaughter period are using of spleen extract biologically active substances were obtained with ultrasound application. The purpose of research — determination of changes of protein fractions, cortisol content in rabbits blood before slaughter and their correction of natural origin biologically active substances (spleen extract).</p> <p>Object and research methods. The experiment was conducted on 15 rabbits with standard diet. Three groups of rabbits five month of age (5 rabbits each) was formed for research. The spleen extract were using as an biologically active substances to the feed rabbits in pre-slaughter period (five days before slaughter). The extracts were applied to feed by aerosol method (70 °alcohol solution of spleen extract volume of 1.4 ml per rabbit) (group I). The rabbits (group II) received to the feed in the same way of 70 °alcohol solution in the same volume. The control group rabbits received the standard feed in the same volume. The feed eating by rabbits was exercised daily. The rabbits ate food completely. The rabbits slaughter was carried out in the morning. The blood plasma protein fractions separation was carried out by horizontal electrophoresis in polyacrylamide gel (PAAG).</p> <p class="Default">Mathematical treatment of the research results worked statistically using the software package Statistica 6.0 and Microsoft Excel for Windows XP. Probability differences was assessed by Student t-test and results considered likely at P ≤ 0.05.</p> <p>Results and discussion. We measured the ratio of blood plasma protein fractions of rabbits, which in addition to the feed fed of natural origin biologically active substances. As a result of research was found that aerosol introduction of the spleen extract to the rabbits feed increases the Prealbumin concentration in rabbits blood plasma of two experimental groups twice (P ≤ 0.05) and 2.8 times (P ≤ 0.01) compared to the control.</p> <p>Cortisol level in rabbits (which further added to the feed of spleen extract (I research group) blood plasma was reliable lower by 40 % (Р&lt;0.05) compared to control, that may to indicate decreasing of stress before slaughter. On the rabbits feeding final stage is necessary to consider a pre-slaughter stress and to apply of natural origin biologically active substances. The results which obtained can to use in researches of stress hormones, such as cortisol, on farm animals for organism resistance increasing, correction and avoid their pre-slaughter stress and improve product quality.</p>

scholarly journals ГОРМОНАЛЬНИЙ ПРОФІЛЬ ТА НЕСПЕЦИФІЧНА РЕЗИСТЕНТНІСТЬ КНУРІВ ЗА УМОВ ПЕРЕДЗАБІЙНОГО СТРЕСУ

2016 ◽  
Vol 6 (01) ◽  
pp. 80-93
Author(s):  
S.S. Grabovskyi ◽  
O.S. Grabovska
Keyword(s):  
Blood Plasma ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Animal Origin ◽  
Control Group ◽  
Control Groups ◽  
Natural Origin ◽  
And Control ◽  
Active Substances

<p>The article is devoted to determination of hormonal profile and nonspecific resistance in boars blood before slaughter after using of biologically active substances — animal origin antistressors andimmunostimulators.</p><p><strong>The purpose</strong><strong> </strong><strong>of research</strong> — determination of changes of insulin, adrenocorticotropic hormone (ACTH), cortisol content in boars blood before slaughter and their correction of natural origin biologically active substances of spleen extract.</p><p><strong>Object and research methods</strong>. The spleen extract has been additionally entered to the boars feed at 5 days before slaughter as an antistressors and immunostimulators in pre-slaughter period. The experiment was conducted on 15 boars with standard diet. Three groups of boars six months of age (5 boars each) were formed for research. The pig’s spleen extract was obtained with ultrasound application (Iresearch group) were using as a biologically active substances to the feed boars in pre-slaughter period. The extracts were applied to dry feed by aerosol method (70 % alcohol solution of spleen extract volume of 1.4 ml per kg body weight). The boars of II research group in the same way received to the feed of 70 % ethanol solution in the same volume. The boars of control group received only dry feed economy. Theboars slaughter was held on day 13 hours a.m. Mathematical treatment of the research results worked statistically using the software package Statistica 6.0.</p><p><strong>Results and discussion</strong>. The ACTH and cortisol level in the boars’ blood plasma of experimental and control groups significantly increased after transportation (before the slaughter) compared with the indexes before transportation to meat plant. The ACTH concentration in the boars’ blood plasma of І experimental group, which was added to the basic diet spleen extract, was 10 % lower than in the control group boars compared with indicators before and after transportation. The insulin concentration of І experimental and control groups boars was higher after transportation with comparing beforetransportation to meat plant.</p><p>All nonspecific body resistance parameters significantly increased in boars blood, which in addition to food received spleen extract, compared with the control group animals after transportation before slaughter: phagocytic activity increased at 12 %, phagocytic index — 13 % and phagocytic number — 14 %.</p><p>On the boars’ feeding final stage is necessary to consider a pre-slaughter stress and to apply of natural origin biologically active substances. The results can be used on other farm animals for resistance increasing, correction and avoid their pre-slaughter stress and improve the product quality.</p>

ECDYSTEROIDS IN NATURE AND THEIR PROPERTIES

2019 ◽  
Vol 5 (3) ◽  
pp. 26-28
Author(s):  
Э. Наумова ◽  
E. Naumova ◽  
Б. Валентинов ◽  
B. Valentinov
Keyword(s):  
Raw Materials ◽  
Folk Medicine ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Natural Sources ◽  
Natural Origin ◽  
Adaptogenic Activity ◽  
Active Substances

Ecdysteroids are biologically active substances of natural origin. Their adaptogenic activity, hormone-like and bioregulatory effects used in traditional and folk medicine are known. Sources of raw materials are diverse. Applications depend on the methods of obtaining ecdysteroids and their natural sources.

scholarly journals SPECTROPHOTOMETRIC DETERMINATION OF ANTIOXIDANT SODIUM METABISULPHITE CONTENT IN PREPARED MEDICINAL FORMS OF VETERINARY MEDICINAL PRODUCTS

2020 ◽  
Vol 21 (2) ◽  
pp. 189-198
Author(s):  
H. Yu. Teslyar ◽  
M. Ya. Smolinska ◽  
I. Ya. Kotsyumbas ◽  
N. M. Chyhyn ◽  
N. G. Rohulia ◽  
...  
Keyword(s):  
Spectrophotometric Determination ◽  
Storage Time ◽  
Analytical Signal ◽  
Veterinary Drugs ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Optimal Conditions ◽  
Sodium Metabisulphite ◽  
Active Substances

An analytical method for the quantitative determination of antioxidant sodium metabisulphite in veterinary drugs has been proposed by spectrophotometric method. Based on the literature data, the optimal conditions of the analytical reaction were selected experimentally. The dependence of the value of the analytical signal on the temperature of the reaction medium, concentration of p-rosaniline and duration of the reaction was investigated to establish the optimal conditions of the analytical reaction and obtain a stable analytical signal. The stability of the colored analytical form in time was also checked and the linear dependence of the value of the analytical signal on the concentration of sodium metabisulphite was investigated. The analytical reaction at room temperature is optimal. The maximum analaytical signal is achieved by carrying out the analytical reaction for 10 minutes and then practically does not change for an hour. To achieve the maximum analytical signal, it is necessary to use a 20-fold excess of dye relative to sodium metabisulphite. The analytical signal remains stable only for the first hour, then gradually begins to decrease. Metrological characteristics of the method of determination of metabisulphite in veterinary drugs are calculated, the limits of spectrophotometric determination are 0.33 – 2.50 μg/ml. The correctness of the developed method was checked on model solutions by the method of "introduced-found" method of comparison in the presence of various biologically active substances that are part of drugs together with sodium metabisulphite. The content of sodium metabisulphite in veterinary drugs of domestic and foreign production at different intervals of their storage time was established. It is shown that the content of sodium metabisulphite in drugs decreases during their storage time, until complete disappearance, which directly affects the content of the active substance, because in the absence of antioxidant oxidative processes with biologically active substances begin to take place.

scholarly journals Flavonoid’s Content Determination in Irradiated Samples via Spectrophotometric Analysis

2019 ◽  
Vol 5 (10) ◽  
pp. 32-39 ◽  
Author(s):  
A. Sarukhanov ◽  
A. Morozova ◽  
N. Vasilyeva ◽  
D. Krylenkin
Keyword(s):  
Quantitative Determination ◽  
Raw Materials ◽  
General Information ◽  
Spectrophotometric Analysis ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Qualitative And Quantitative ◽  
Content Determination ◽  
Active Substances

General information about biologically active substances flavonoids is concisely presented. The need for the quantitative determination of its content in products and the influence of product processing on flavonoid’s content is justified. Methods of qualitative and quantitative determination of flavonoids content in various products, medications and raw materials were studied. Based on this study the method of quantitative determination of flavonoid’s in samples of irradiated coriander is compiled and tested. Further ways to improve and adjust the compiled method for flavonoid’s content determination in irradiated samples via spectrophotometric analysis is considered.

A kinetic determination of ultramicroquantities of some biologically active substances

1980 ◽  
Vol 25 (3) ◽  
pp. 380-387 ◽  
Author(s):  
G.A. Milovanović ◽  
T.J. Janjić ◽  
S. Petrović ◽  
G. Kuzmanović
Keyword(s):  
Biologically Active Substances ◽  
Biologically Active ◽  
Kinetic Determination ◽  
Active Substances
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