scholarly journals Using time lapse monitoring of embryo development (TLMED) in reproductive biotechnology

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
M. Sharan ◽  
S. Shalovylo ◽  
C. Grymak
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Assisted Reproductive Technologies ◽  
New Technology ◽  
Time Lapse ◽  
Reproductive Technologies ◽  
New Techniques ◽  
Vitro Fertilization ◽  
Animal Reproduction

An increasing interest in assisted reproductive technologies and their applications in biotechnology of animal reproduction is currently observed. The development of in vitro fertilization (IVF) has led to the emergence of new techniques as ICSI (Intracytoplasmic Sperm Iniection), 1MSI (Morphologically Selected Intracytoplasmic Sperm Iniection) and PGS (Pre–Implantation Genetic Screening). Recently a new technology TLMED (Time Lapse Monitoring of Embryo Development), which allows to observe dynamics of embryo development is being introduced to practice. The application of this technique allows to determine the morphokinetics parameters of normal embryos and to observe its development more accurately. Currently, there are four systems in the market based on this technique: Primo Vision (Vitrolife, Sweden), EEVA (Auxogyn, USA), Embryoscope (Vitrolife, Sweden), MIRI (Esco, Denmark), which conducting the morphokinetics analysis of embryos, which ensures selection of the best embryos and increases the effectiveness of in vitro fertilization. The first positive results of the use of system TLMED in agriculture biotechnology on the example of the Biomedical Research Center of the Warsaw University of Life Sciences predicts its introduction into the everyday practice of veterinary clinics. The aim of this paper is to present each system and review the existing information about the possible application of TLMED and the usefulness in animal reproductive biotechnology.

Legal restrictions on pre-implantation genetic diagnostics in the framework of in vitro fertilization procedure: foreign experience and Russian perspective

2019 ◽  
Author(s):  
N.A. Altinnik , S.S. Zenin , V.V. Komarova et all
Keyword(s):  
In Vitro Fertilization ◽  
Assisted Reproductive Technologies ◽  
Genetic Diseases ◽  
Genetic Diagnosis ◽  
Reproductive Technologies ◽  
Legal Regulation ◽  
Genetic Diagnostics ◽  
Vitro Fertilization ◽  
Medical Indications

The article discusses the factors that determine the content of the legal limitations of pre-implantation genetic diagnosis in the framework of the in vitro fertilization procedure, taking into account international experience and modern domestic regulatory legal regulation of the field of assisted reproductive technologies. The authors substantiates the conclusion that it is necessary to legislate a list of medical indications for preimplantation genetic diagnosis, as well as the categories of hereditary or other genetic diseases diagnosed in the framework of this procedure.

scholarly journals Prevalence of pathogenic copy number variants among children conceived by donor oocyte

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sandra Monfort ◽  
Carmen Orellana ◽  
Silvestre Oltra ◽  
Mónica Rosello ◽  
Alfonso Caro-Llopis ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Copy Number ◽  
Assisted Reproductive Technologies ◽  
Copy Number Variants ◽  
Reproductive Technologies ◽  
Significant Excess ◽  
Vitro Fertilization ◽  
Donor Oocyte ◽  
Increased Risk

AbstractDevelopment of assisted reproductive technologies to address infertility has favored the birth of many children in the last years. The majority of children born with these treatments are healthy, but some concerns remain on the safety of these medical procedures. We have retrospectively analyzed both the fertilization method and the microarray results in all those children born between 2010 and 2019 with multiple congenital anomalies, developmental delay and/or autistic spectrum disorder (n = 486) referred for array study in our center. This analysis showed a significant excess of pathogenic copy number variants among those patients conceived after in vitro fertilization with donor oocyte with respect to those patients conceived by natural fertilization (p = 0.0001). On the other hand, no significant excess of pathogenic copy number variants was observed among patients born by autologous oocyte in vitro fertilization. Further studies are necessary to confirm these results and in order to identify the factors that may contribute to an increased risk of genomic rearrangements, as well as consider the screening for genomic alterations after oocyte donation in prenatal diagnosis.

scholarly journals GAMETE DONATION. ETHICAL AND LEGAL ASPECTS

2020 ◽  
pp. 13-19
Author(s):  
L. Chalova ◽  
V. Lokshin ◽  
A. Guseva ◽  
A. Kinzhibayev
Keyword(s):  
In Vitro Fertilization ◽  
Literature Review ◽  
World Literature ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Legal Aspects ◽  
Gamete Donation ◽  
Vitro Fertilization ◽  
National Legislation

This world literature review tries to determine the significance of the gamete donation in the field of assisted reproductive technologies as well as the availability of treatment methods using donation in in vitro fertilization programs. Gamete donation is regulated by every country's national legislation system, and quite often the laws vary between the states. There are practically no universal standards and/or rules in this area, which, in turn, leads to an ambivalent reaction towards reproductive practices.

scholarly journals 266IN VITRO-DERIVED EMBRYO PRODUCTION WITH SEXED AND UNSEXED SEMEN FROM DIFFERENT BULLS

2004 ◽  
Vol 16 (2) ◽  
pp. 253 ◽  
Author(s):  
L. Ferré ◽  
C. Ohlrichs ◽  
D. Faber
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Calf Serum ◽  
Sperm Concentration ◽  
Reproductive Technologies ◽  
Chi Square ◽  
Vitro Fertilization ◽  
Sexed Semen ◽  
Chi Square Analysis

The production of pre-sex-selected calves by in vitro fertilization (IVF), using sexed semen, does show some benefits due to the small quantity of sperms needed for the process as compared to other reproductive technologies. The objective of this study was to determine differences among bulls and sperm concentrations in embryo development with sexed and unsexed semen. Follicles ranging from 2 to 6mm in diameter were aspirated from slaughterhouse ovaries. COC were selected and matured in groups of maximum of 30 in 1.8mL of TCM-199, supplemented with 10% fetal calf serum, 0.01UmL−1 bFSH, 0.01UmL−1 bLH and 10μLmL−1 penicillin-streptomycin for 24h at 38.5°C. Fertilization (Day 0) was carried out in micro-drops (50μL) with TALP-FERT medium containing PHE (3μgmL−1 penicillamine, 11μgmL−1 hypotaurine and 0.18μgmL−1 epinephrine), 10μLmL−1 non-essential amino acid and 2μgmL−1 heparin. Frozen/thawed sexed (female) and non-sexed sperms from five bulls were selected in a discontinuous percoll gradient. Sperm concentration was 1×106 for non-sexed semen and 1×106 or 2×106 for sexed semen. After 18–20h, presumptive zygotes were denuded and cultured in groups of 10 in 50-μL micro-drops of SOF citrate with 5% FCS (Holm P et al., 1999 Theriogenology 52, 683–700) under paraffin oil in a 5% O2, 5% CO2, 90% N2 atmosphere with high humidity. On Day 7, blastocysts (BL) were morphologically evaluated and recorded. Results are shown in Table 1. Data was compared by chi-square analysis. Sexed frozen bovine sperm can be used successfully in IVF systems. More research needs to be done to optimize and standardize bovine in vitro fertilization with sexed semen. Table 1 Results of comparisons between bulls, sperm concentrations, cleavage and embryo development

scholarly journals Islam, Assisted Reproductive Technologies, and the Middle Eastern State

1970 ◽  
Author(s):  
Marcia C. Inhorn
Keyword(s):  
In Vitro Fertilization ◽  
Assisted Reproductive Technologies ◽  
Middle Eastern ◽  
Reproductive Technologies ◽  
Third Party ◽  
Vitro Fertilization ◽  
Eastern State ◽  
Nation States

Assisted reproductive technologies, such as in vitro fertilization, have made their way to the Middle Eastern nation states. However, in the Sunni-dominant countries, third-party donation of sperm, eggs, embryos, and uteruses (as in surrogacy) is banned, leading some Sunni Muslim couples to travel to Iran and Lebanon, where Shia Muslim clergy have allowed donor technologies.

Assisted reproductive technologies and their impact on a woman's body

2021 ◽  
pp. 239-242
Author(s):  
A.A. Fil ◽  
◽  
E.L. Sorokin ◽  
O.V. Kolenko ◽  
◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Vascular Pathology ◽  
Timely Manner ◽  
Fertilization Program ◽  
Vitro Fertilization ◽  
Hemostatic System ◽  
Retinal Pathology

A review of the literature data is presented, which indicates the possible influence of assisted reproductive technologies (ART) on the formation of both systemic and retinal vascular pathology. Despite the fact that the overall incidence of thromboembolic complications as a result of the use of ART is low, nevertheless, in women included in the in vitro fertilization program, their risk increases by 10 times. Therefore, due to the adverse effect of these drugs on the hemostatic system of a woman, ophthalmologists should be more wary of them in order to detect vascular retinal pathology in a timely manner. Key words: assisted reproductive technologies, in vitro fertilization, hyperestrogenism, vascular retinal pathology.

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