scholarly journals Evaluation of a Consciousness Energy Healing Based Test Formulation for Skin Health

2019 ◽  
Vol 5 (1) ◽  
pp. 7-13
Author(s):  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Skin Color ◽  
Untreated Group ◽  
Cosmetic Products ◽  
Wound Area ◽  
Skin Cells ◽  
Exposure Data ◽  
Test Formulation ◽  
Energy Healing ◽  
Brdu Assay ◽  
Skin Quality

This study was investigated the effect of Consciousness Energy Healing (The Trivedi Effect®) based test formulation and DMEM medium on skin health in B16-F10, HaCaT, and HFF-1 cells. The test formulation and DMEM were assigned into two parts. First part was received the Biofield Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and were defined as the Biofield Treated samples (BT), while the second parts were denoted as the untreated test samples (UT). MTT assay data showed more than 76 % cells were viable in all the tested concentrations in three different cell lines, indicating that the test formulation was safe and nontoxic. The BrdU assay showed the percent cell proliferation was increased by 15.88 % in the UT-DMEM + BT- Test formulation compared to the UT-DMEM + UT- Test formulation group. Elastin was increased significantly (p ≤ 0.001) by 64.37 %, 29.46 % and 38.75 % in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation, respectively at 100 µg / mL compared to the untreated test formulation. Hyaluronic acid was significantly increased by 17.74 %, 96.20 %, and 25.03% in UT-DMEM + BT- Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 10 µg / mL compared to untreated group. The level of melanin was significantly decreased by 8.60% (p ≤ 0.05), 12.68 % (p ≤ 0.001), and 21.73% (p ≤ 0.001) in the UT-DMEM + BT- Test formulation group at 0.1, 1.0, and 10 µg / mL, respectively compared to the untreated group. Protection of skin cells after UV-B exposure data showed that the cell viability was significantly increased by 31.15 % and 10.84 % in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 50 µg / mL compared to the untreated group. Wound healing data exhibited that the wound area was recovered with new cells by 84.91 %, 92.31 %, 35.09 %, and 41.67 % at 10, 50, 100, and 200 µg / mL in the BT-DMEM + BT- Test formulation group, respectively in Ha Ca T cells compared to the untreated group. The experimental data envisaged that the Biofield Energy Treated DMEM and test formulation showed significant skin protective activity. Thus, the Biofield Energy Treated test formulation could be useful for the development of effective cosmetic products for the nourishment of several skin problems such as fine lines, wrinkles, texture, pore size, elasticity, skin color / clarity, redness, hydration, to maintain the overall skin quality, etc.

scholarly journals Elasticity Profile of Skin, Neuronal, Cardiac, and Skeletal Muscle Cells after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation

2021 ◽  
Vol 2 (4) ◽  
pp. 19-29
Author(s):  
Mahendra Kumar Trivedi ◽  
Alice Branton ◽  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Cell Line ◽  
Test Group ◽  
Untreated Group ◽  
Alpha Tocopherol ◽  
H9c2 Cells ◽  
Human Neuroblastoma ◽  
Rat Cardiomyocytes ◽  
Test Items ◽  
Test Formulation ◽  
Energy Healing

The present study aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on elasticity of skin, heart, muscle, and neuronal cells in the H9C2 (rat cardiomyocytes), C2C12 (mouse myoblast cells), HaCaT (human keratinocytes), and SH-SY5Y (human neuroblastoma cells) cell line in DMEM medium. The test formulation constituents were divided into two parts; one section was defined as untreated test formulation (UT), while the other portion of test formulation received Biofield Energy Healing/Blessing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing/Blessing Treatment and divided as Biofield Energy Treated/Blessed (BT) and untreated (UT) test items. MTT data showed that the test formulation in various concentrations was found as safe and nontoxic in the tested concentrations with viability range from 73% to 307%. Young’s modulus (YM) is a measure of cell stiffness, a decrease in YM value indicates increase elasticity of the cells and vice-versa. YM in H9C2 cells were decreased by 9.6% and 66.1% in the BT-DMEM + UT-TI group at 0.1 and 1 µg/mL respectively, as compared with untreated test group. However, C2C21 cells showed increased YM by 443.9% at 1 µg/mL in the UT-DMEM + BT-TI group, while 869.6% increased YM in the BT-DMEM + UT-TI group at 1 µg/mL as compared with untreated test group. However, 314% increased YM was reported in the BT-DMEM + BT-TI group at 1 µg/mL as compared with the untreated test group. However, the value of YM was significantly decreased in the HaCaT cell line by 247.7% (at 1 µg/mL), 225.8% (at 0.1 µg/mL), and 97.9% (at 1 µg/mL) in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. In addition, YM was significantly decreased in the SH-SY5Y cell line by 92.6%, 18.1%, and 26.6% at 1 µg/mL in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. Overall, the results showed the significant decreased YM among the SH-SY5Y, HaCaT, and H9C2 cells, while it was increased in the C2C21 cell line. Thus, the mechanical properties of cells such as cellular function, including shape, motility, differentiation, division, and adhesion to its surrounding extracellular matrix were improved. Overall, it can be useful in many disease progressions with improved cellular elasticity and its associated complications/symptoms.

scholarly journals The efficacy of skin care products containing glutathione in delivering skin lightening in Indonesian women

2019 ◽  
Author(s):  
Kristiana Etnawati ◽  
Dwi Retno Adiwinarni ◽  
Devi Artami Susetiati ◽  
Yusuke Sauchi ◽  
Hitomi Ito
Keyword(s):  
Skin Color ◽  
Skin Care ◽  
Efficacy And Safety ◽  
Facial Skin ◽  
Cosmetic Products ◽  
Double Blind ◽  
Tingling Sensation ◽  
Asian Market ◽  
Skin Care Products ◽  
Skin Lightening

Glutathione is a potent antioxidant, which is widely used in skin care products for skin lightening especially in the Asian market. Presently, there was only one study of topical 2% oxidized glutathione (GSSG) in Philipino women, further study is needed to evaluate the efficacy and safety of cosmetic products containing glutathione. A double blind randomized clinical trial study was conducted in Yogyakarta, Indonesia, in 74 healthy Indonesian women, with average age 33.3 ± 5.9 years when subjects participated in the study. Each subject received supervised applications of Facial Wash twice a day, Day Cream with sunscreen and Night Cream. The subjects were divided into 3 groups based on the active ingredients of the tested products, which included glutathione reduced (GSH) 0.1%, GSH 0.5%, and without GSH. The effects of the tested products in lightening skin color and pigmentation were measured colorimetry with Chromameter Minolta for L*. Compared to a baseline, there were significant increases of L* (lightness) or ΔL* detected as early as week 2 in which group of GSH 0.1% were significantly higher compared to group of GSH 0.5% and group without GSH. Hyperpigmented lesions also improved, where GSH 0.5% group showed its superiority compared to others groups in week 8. The undesired effect were few minutes mild tingling sensation after night cream, and worsening of acne in all tested products. Conclusion skin care products containing GSH 0.1% and 0.5% were effective in facial skin lightening.

scholarly journals Evaluation of Immunomodulatory Effect of a Novel Test Formulation in D-Galactose-Induced Aging Dysfunction in Sprague Dawley Rats

2021 ◽  
Vol 4 (1) ◽  
pp. 12-29
Author(s):  
Mahendra Kumar Trivedi ◽  
Alice Branton ◽  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Treatment Strategies ◽  
Immunomodulatory Effect ◽  
Immunomodulatory Activity ◽  
Sprague Dawley ◽  
Test Formulation ◽  
Immune Biomarkers ◽  
Sprague Dawley Rats ◽  
Hematological Analysis ◽  
Per Se ◽  
Energy Healing

The aim of the study was to evaluate the immunomodulatory activity of the Biofield Treated/Blessed proprietary test formulation consisting of essential ingredients viz. minerals (zinc, magnesium, iron, and copper) and vitamins (B6, B12, and D3) in male Sprague Dawley rats. Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Healing Treatment/Blessing, while the other part was defined as the Biofield Energy Treated/Blessed sample, which received the Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi remotely. Additionally, three group of animals were also received Biofield Energy Treatment per se (at day -15) under similar conditions. The parameters were assessed such as immune biomarkers (IgM, IgG, IgA, IgE, CD4+, CD8+, and CD28+), biochemistry and hematology and histopathology. The experimental results showed IgG level was significantly increased by 10.70% and 8.03% in the G6 (Biofield Energy Treatment per se at day -15) and G8 (Biofield Treatment per se to animals plus Biofield Treated test formulation from day -15) groups, respectively as compared with untreated test formulation (G4). Additionally, CD8+ count was significantly increased by 20.67% in the G8 group, while CD28+ count was significantly increased by 11.70%, 8.32%, and 9.82% in the G7 (Biofield Energy Treated test formulation at day -15), G8, and G9 (Biofield Treatment per se (day -15) to animals plus untreated test formulation) groups, respectively after Biofield Energy Treatment to the animals as compared with the untreated test formulation. In hematological analysis, platelet count was increased in the G5, G6, G7, G8, and G9 groups by 40.69%, 27.95%, 26.67%, 38.58%, and 28.28%, respectively compared with the disease control (G2) group. Biochemical parameters showed significant decrease in the level of creatinine by 32.14% in the G9 group as compared with the G2 group. Further, animal body weight, feed intake, relative organ weight, and histopathological findings of all the tested groups did not show any abnormal findings with respect to the safe and non-toxic treatment strategies. Overall, the experimental data concluded that the Biofield Energy Treated/Blessed test formulation showed considerable improved cellular and humoral immune response as compared with the untreated test formulation. Thus, the Trivedi Effect®-Biofield Energy Healing Treatment per se and the test formulation has the significant capacity for immunomodulatory effect, stress management and anti-aging by improving overall health.

Exposure data for cosmetic products: lipstick, body lotion, and face cream

2005 ◽  
Vol 43 (2) ◽  
pp. 279-291 ◽  
Author(s):  
L.J. Loretz ◽  
A.M. Api ◽  
L.M. Barraj ◽  
J. Burdick ◽  
W.E. Dressler ◽  
...  
Keyword(s):  
Cosmetic Products ◽  
Exposure Data

scholarly journals Postharvest Stimulation of Skin Color in Royal Gala Apple

1995 ◽  
Vol 120 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Yi hu Dong ◽  
Deepali Mitra ◽  
Arend Kootstra ◽  
Carolyn Lister ◽  
Jane Lancaster
Keyword(s):  
Skin Color ◽  
High Performance ◽  
Phenylalanine Ammonia Lyase ◽  
De Novo ◽  
Northern Analysis ◽  
Chromatography Analysis ◽  
Skin Cells ◽  
Red Color ◽  
Irradiation Treatment ◽  
Dose Dependent

The red color of Royal Gala apple (Malus domestics Borkh.) skin increased in intensity following irradiation with ultraviolet (UV) and white light. The enhanced red apple color was due to an increase in anthocyanin concentration and the increase was dose dependent. High-performance liquid chromatography analysis showed that the composition of flavonoids in UV treated and natural red colored apple skins was similar. The red apple skin color further increased after storage at 4C in the dark. During the course of irradiation the enzymatic activities of phenylalanine ammonia lyase (PAL) and chalcone isomerase (CHI) increased 10-to 20-fold. Northern analysis showed an increase in PAL transcripts during the irradiation treatment, suggesting that the increase in PAL enzymatic activity was due to de novo synthesis of the enzyme in apple skin cells.

scholarly journals Evaluation of Anti-oxidation and Therapeutic Effect of Biofield Energy Healing Based Novel Test Formulation Using TNBS (Tri Nitro Benzene Sulfonic Acid) - Induced Ulcerative Colitis in Sprague Dawley Rats

2021 ◽  
Vol 2 (1) ◽  
pp. 29-40
Author(s):  
Mahendra Kumar Trivedi ◽  
Snehasis Jana
Keyword(s):  
Inflammatory Diseases ◽  
Standard Procedure ◽  
Sprague Dawley ◽  
Colon Tissue ◽  
Activity Data ◽  
Oxidation Activity ◽  
Benzene Sulfonic Acid ◽  
Test Formulation ◽  
Per Se ◽  
Energy Healing

The aim of the study was to evaluate the antioxidant potential of Biofield Energy Healing (the Trivedi Effect®) based test formulation using TNBS-induced colitis animal model. Each ingredient of the test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was treated with Biofield Energy Treatment by Mr. Mahendra Kumar Trivedi and defined as the Biofield Energy Treated test formulation. The colon tissue was used for the estimation of anti-oxidation activity for catalase (CAT), glutathione (GSH), lipid peroxidation (LPO) product, myeloperoxidase (MPO), superoxide dismutase (SOD), and glutathione peroxidase (GPx) using standard procedure. The antioxidant results showed that the CAT level was significantly increased by 95.4% (p≤0.001), 72.3%, 47.6%, and 13.9% in the Biofield Energy Treated test formulation (G5), Biofield treatment per se to animals (-15 days)(G6), Biofield Energy Treatment per se to animals plus Biofield Energy Treated test formulation (-15 day) (G8), and Biofield Energy Treatment per se to animals plus untreated test formulation (G9) groups, respectively as compared to the untreated test formulation group (G4). Further, colon GSH activity was found to be significantly increased by 23.2% (p≤0.05) 15.4%, and 15.5%, in G5, G6, and G9 groups, respectively with respect to G2 group. In addition, colon LPO activity data suggested that it was decreased by 12%, 17%, 18%, and 19.1% in G5, G6, Biofield Energy Treated test formulation (-15 day) (G7), and G8 groups, respectively, as compared with the G2 group. The level of MPO showed a significant (p≤0.001) reduced level by 27.9%, 22%, 14.5%, 16.6%, and 25.3% in G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. The level of colon SOD was increased by 16.7% and 14.2% in the G5 and G9 groups, respectively as compared with the untreated test formulation, G4 group. Colon GPx level was increased by 177.6%, 71.4%, 71.4%, 161.2%, and 114.3% in G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. Thus, it can be concluded that the Trivedi Effect®-Consciousness Energy Healing based test formulation and Biofield Energy per se has significant colon anti-oxidation profile, which can be used to improve many autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

scholarly journals Skin Color in Apples—Influence of Copigmentation and Plastid Pigments on Shade and Darkness of Red Color in Five Genotypes

1994 ◽  
Vol 119 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Jane E Lancaster ◽  
Jan E. Grant ◽  
Carolyn E. Lister ◽  
Michael C. Taylor
Keyword(s):  
Skin Color ◽  
Red Cells ◽  
Skin Cells ◽  
Color Quality ◽  
Red Color ◽  
Significant Difference ◽  
The Mean ◽  
Plastid Pigments ◽  
Red Coloration

The biochemical and cytological mechanisms responsible for the differences in red color quality of apples (Malus domestics Borkh.) were investigated. Copigmentation, the increase in absorbance maxima (λ max) from anthocyanin and flavonoid interactions, is known to be a mechanism for producing variation in shade of red in flowers. In intact apple skin cells, the mean λ max was 550 nm, with no significant difference between genotypes. Furthermore, the ratio of flavonols and proanthocyanidins to anthocyanins was similar for all genotypes. Therefore, copigmentation is not a mechanism producing different shades of red in apples. Darkness of red skin was positively related to the proportion of red cells in the skin and the size of the vacuoles containing anthocyanins. Measurements of plastid pigments, chlorophyll, and carotenoids, compared with L*, a*, b* measurements, indicated that the visual blending of plastid pigments and anthocyanins has an important influence on red coloration of apple skin.

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