scholarly journals Elasticity Profile of Skin, Neuronal, Cardiac, and Skeletal Muscle Cells after Treatment with the Biofield Energy Healing-Based Proprietary Test Formulation

2021 ◽  
Vol 2 (4) ◽  
pp. 19-29
Author(s):  
Mahendra Kumar Trivedi ◽  
Alice Branton ◽  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Cell Line ◽  
Test Group ◽  
Untreated Group ◽  
Alpha Tocopherol ◽  
H9c2 Cells ◽  
Human Neuroblastoma ◽  
Rat Cardiomyocytes ◽  
Test Items ◽  
Test Formulation ◽  
Energy Healing

The present study aimed to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation/item (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on elasticity of skin, heart, muscle, and neuronal cells in the H9C2 (rat cardiomyocytes), C2C12 (mouse myoblast cells), HaCaT (human keratinocytes), and SH-SY5Y (human neuroblastoma cells) cell line in DMEM medium. The test formulation constituents were divided into two parts; one section was defined as untreated test formulation (UT), while the other portion of test formulation received Biofield Energy Healing/Blessing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing/Blessing Treatment and divided as Biofield Energy Treated/Blessed (BT) and untreated (UT) test items. MTT data showed that the test formulation in various concentrations was found as safe and nontoxic in the tested concentrations with viability range from 73% to 307%. Young’s modulus (YM) is a measure of cell stiffness, a decrease in YM value indicates increase elasticity of the cells and vice-versa. YM in H9C2 cells were decreased by 9.6% and 66.1% in the BT-DMEM + UT-TI group at 0.1 and 1 µg/mL respectively, as compared with untreated test group. However, C2C21 cells showed increased YM by 443.9% at 1 µg/mL in the UT-DMEM + BT-TI group, while 869.6% increased YM in the BT-DMEM + UT-TI group at 1 µg/mL as compared with untreated test group. However, 314% increased YM was reported in the BT-DMEM + BT-TI group at 1 µg/mL as compared with the untreated test group. However, the value of YM was significantly decreased in the HaCaT cell line by 247.7% (at 1 µg/mL), 225.8% (at 0.1 µg/mL), and 97.9% (at 1 µg/mL) in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. In addition, YM was significantly decreased in the SH-SY5Y cell line by 92.6%, 18.1%, and 26.6% at 1 µg/mL in the UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI group respectively, as compared with the untreated group. Overall, the results showed the significant decreased YM among the SH-SY5Y, HaCaT, and H9C2 cells, while it was increased in the C2C21 cell line. Thus, the mechanical properties of cells such as cellular function, including shape, motility, differentiation, division, and adhesion to its surrounding extracellular matrix were improved. Overall, it can be useful in many disease progressions with improved cellular elasticity and its associated complications/symptoms.

scholarly journals Evaluation of Stress Biomakers (Cytokines) in Presence of Unpredictable Chronic Stress (UCS) in Sprague Dawley Rats after Treatment with the Biofield Energy Treated Novel Proprietary Test Formulation

2021 ◽  
Vol 4 (2) ◽  
Author(s):  
Snehasis Jana
Keyword(s):  
Chronic Stress ◽  
Preventive Treatment ◽  
Alpha Tocopherol ◽  
Control Group ◽  
Sprague Dawley ◽  
Ginseng Extract ◽  
Test Formulation ◽  
Per Se ◽  
Energy Healing ◽  
Α Level

Unpredictable chronic stress (UCS) model was used to evaluate the effect of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats for the estimation of serum cytokines such as TNF-Alpha, IFN-Gamma, IL-6, and IL-2, along with C-reactive protein (CRP) using ELISA assay. A test formulation was formulated including minerals (calcium, copper, iron, magnesium, selenium, and zinc), vitamins (ascorbic acid, alpha tocopherol, cholecalciferol, cyanocobalamin, and pyridoxine HCl), cannabidiol isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one part of each ingredient was defined as the untreated test formulation, while the other parts and three groups of animal received Biofield Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The cytokines estimation results showed that the level of IL-2 was reduced by 14.2% in Biofield Energy Treatment per se to the rats (G6) group as compared with the disease control group (G2). TNF-α level in serum was decreased by 11% in the G6 group as compared with the G2 group. IL-6 level in serum was decreased by 21.7%, 30.8%, 16.3%, 10.1%, and 13.4% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. However, IFN-γ level was decreased by 18.3% and 11% in the G6 and G9 groups, respectively as compared with the G4. Similarly, CRP level was significantly decreased by 25.8%, 15.2%, and 14.6% in the G5, G6, and G7 groups respectively, as compared with the G4. All-inclusive, Mr. Trivedi’s Biofield Energy Healing Treatment/Blessing per se along with other preventive treatment groups showed a significant impact on various inflammatory disorders (gout, inflammatory arthritis myositis, rheumatoid arthritis, scleroderma, etc.). Therefore, the outcomes could be slowdown the stress-related disease progression and its complications/symptoms in the preventive maintenance groups (viz. G6, G7, G8, and G9).

scholarly journals Biofield Energy Therapy: Role in Multiple Organ Health Specific Biomarkers in Cell-Based Assay

2019 ◽  
Vol 2 (2) ◽  
Author(s):  
Snehasis Jana
Keyword(s):  
Cell Viability ◽  
Hepg2 Cells ◽  
Test Group ◽  
Multiple Organ ◽  
Untreated Group ◽  
Specific Cell ◽  
Sod Activity ◽  
Cellular Protection ◽  
Test Formulation ◽  
Alt Activity

The major cause of high rate of mortality is the multiple organ dysfunction among critical care healthcare services. The aim of the current study was to evaluate the impact of the Biofield Energy Treated test formulation using standard and specific cell lines related with vital organs functioning. The test formulation and the specific cell media was divided into two parts; one part was untreated (UT) and other part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Sakina Aleemah Ansari, USA and labeled as the Biofield Energy Treated (BT) test formulation/media. The test formulation was tested against various activities using cell line assay in their specific medium (Med). The test formulation was tested for cell viability, and the results showed that the test formulation at tested concentrations was found safe and non-toxic. Cytoprotective activity showed improved cellular restoration by 105.4% (at 25 µg/mL), 32.8% (at 25 µg/mL), and 151.8% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BTMed + BT-TI groups respectively, as compared to the untreated test group in the human cardiac fibroblasts cells (HCF) cells, while improved restoration of cell viability by 22.4% (at 25.5 µg/mL), 67.1% (at 10 µg/mL), and 72.9% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups respectively, as compared to the untreated test group in HepG2 cells. Cellular restoration in A549 cells was improved by 9.3%, 70.4%, and 14.1% at 0.1, 1, and 25.5 µg/mL respectively, in the BT-Med + UT-TI group, while 3% and 4.6% improved cellular restoration was reported at 10 and 25.5 µg/mL respectively, at BT-Med + BT-TI groups as compared to the untreated test group. ALP activity in Ishikawa cells was significantly increased by 68.4%, 41.1%, and 18.8% at 0.1, 10, and 50 µg/mL respectively, in the UT-Med + BTTI group, while in MG-63 cells showed maximum increased ALP activity by 92.7%, 84.5%, and 93.2% respectively in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI group respectively, at 50 µg/mL as compared to the untreated group. The maximum percent cellular protection of HCF (heart) cells (decreased of LDH activity) was significantlyincreased by 51.6%, 88.7%, and 53.7% in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups respectively, at 10 µg/mL as compared to the untreated group. Alanine amino transferase (ALT) activity was reported in terms of percent cellular protection of HepG2 (liver) cells. The test data showed improved HepG2 cells protection (represents decreased ALT activity) by 33%, 90.2%, and 72.1% in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups respectively, at 25 µg/mL as compared to the untreated test group. Percentage cellular protection of A549 (lungs) cells (represents increased of SOD activity) was increased by 21.5% at 25.5 µg/mL in the UT-Med + BT-TI, while 33.4%, 25.8%, and 21.5% at 1, 10, and 25.5 µg/mL respectively, in the BT-Med + UT-TI group, and 12.8% increased SOD activity at 25.5 µg/mL in the BT-Med + BT-TI groups as compared to the untreated group. Serotonin level was significantly increased 137.4% (at 0.1 µg/mL), 65.4% (at 0.1 µg/mL), and 77.3% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UTTI, and BT-Med + BT-TI groups, respectively compared to untreated test group in human neuroblastoma cells (SH-SY5Y). However, the relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 24.2% (at 1 µg/mL), 213.7% (at 10 µg/mL), and 328.7% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively as compared to the untreated in MG-63 cells. Thus, the data concluded that The Trivedi Effect® would be significantly useful for improving multiple organs health, which can be used for many coronary artery diseases, arrhythmias, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, asthma, chronic bronchitis, cystic fibrosis, osteoporosis, etc.

scholarly journals Evaluation of a Consciousness Energy Healing Based Test Formulation for Skin Health

2019 ◽  
Vol 5 (1) ◽  
pp. 7-13
Author(s):  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Skin Color ◽  
Untreated Group ◽  
Cosmetic Products ◽  
Wound Area ◽  
Skin Cells ◽  
Exposure Data ◽  
Test Formulation ◽  
Energy Healing ◽  
Brdu Assay ◽  
Skin Quality

This study was investigated the effect of Consciousness Energy Healing (The Trivedi Effect®) based test formulation and DMEM medium on skin health in B16-F10, HaCaT, and HFF-1 cells. The test formulation and DMEM were assigned into two parts. First part was received the Biofield Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and were defined as the Biofield Treated samples (BT), while the second parts were denoted as the untreated test samples (UT). MTT assay data showed more than 76 % cells were viable in all the tested concentrations in three different cell lines, indicating that the test formulation was safe and nontoxic. The BrdU assay showed the percent cell proliferation was increased by 15.88 % in the UT-DMEM + BT- Test formulation compared to the UT-DMEM + UT- Test formulation group. Elastin was increased significantly (p ≤ 0.001) by 64.37 %, 29.46 % and 38.75 % in UT-DMEM + BT-Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation, respectively at 100 µg / mL compared to the untreated test formulation. Hyaluronic acid was significantly increased by 17.74 %, 96.20 %, and 25.03% in UT-DMEM + BT- Test formulation, BT-DMEM + UT-Test formulation, and BT-DMEM + BT-Test formulation groups, respectively at 10 µg / mL compared to untreated group. The level of melanin was significantly decreased by 8.60% (p ≤ 0.05), 12.68 % (p ≤ 0.001), and 21.73% (p ≤ 0.001) in the UT-DMEM + BT- Test formulation group at 0.1, 1.0, and 10 µg / mL, respectively compared to the untreated group. Protection of skin cells after UV-B exposure data showed that the cell viability was significantly increased by 31.15 % and 10.84 % in the UT-DMEM + BT-Test formulation and BT-DMEM + UT-Test formulation groups, respectively at 50 µg / mL compared to the untreated group. Wound healing data exhibited that the wound area was recovered with new cells by 84.91 %, 92.31 %, 35.09 %, and 41.67 % at 10, 50, 100, and 200 µg / mL in the BT-DMEM + BT- Test formulation group, respectively in Ha Ca T cells compared to the untreated group. The experimental data envisaged that the Biofield Energy Treated DMEM and test formulation showed significant skin protective activity. Thus, the Biofield Energy Treated test formulation could be useful for the development of effective cosmetic products for the nourishment of several skin problems such as fine lines, wrinkles, texture, pore size, elasticity, skin color / clarity, redness, hydration, to maintain the overall skin quality, etc.

scholarly journals Impact of Biofield Energy Treatment Based Test Formulation on Vital Organ Health Specific Biomarkers Using Cell Line Study

2019 ◽  
Vol 1 (2) ◽  
pp. 22-36
Author(s):  
Ariadne Esmene Afaganis ◽  
Mahendra Kumar Trivedi ◽  
Alice Branton ◽  
Dahryn Trivedi ◽  
Gopal Nayak ◽  
...  
Keyword(s):  
Cell Lines ◽  
Test Group ◽  
Multiple Organ ◽  
Untreated Group ◽  
Heart Cells ◽  
Specific Cell ◽  
Cellular Protection ◽  
Alp Activity ◽  
Test Formulation ◽  
The Impact

Multiple organ dysfunction syndrome or failure is one of the major concerns against healthcare services in order to maintain the normal function. The present study aimed to explore the impact of the Biofield Energy Treated test formulation on the function of vital organs such as bones, heart, liver, lungs, and brain using standard activity parameters in specific cell-based assays. The test formulation and cells medium was divided into two parts, one untreated (UT) and other part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Ariadne Esmene Afaganis, Canada and was labeled as the Biofield Treated (BT) test formulation/media. The test formulation was tested for cell viability, and the data suggested that the test formulation was found safe and non-toxic against all the cell lines. Cytoprotective activity among the experimental groups showed a significant improved activity by 94.4% at 1 µg/mL in untreated medium (UT-Med) + Biofield Treated Test Item (BT-TI) group in human cardiac fibroblasts cells (HCF) cells, while 84.4% at 10 µg/mL in BT-Med + BT-TI groups in human hepatoma cells (HepG2), and 124% increased cytoprotective action at 1 µg/mL in UT-Med + BT-TI group in adenocarcinomic human alveolar basal epithelial cells (A549) cells as compared with the untreated test group. ALP activity in MG-63 cells was significantly increased by 85.9% at 10 µg/mL in the UT-Med + BT-TI group, while in Ishikawa cells showed maximum increased ALP activity by 59.2% at 0.1 µg/mL in BT-Med + BT-TI groups as compared to the untreated group. The percent protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 53% and 40.5% at 1 and 10 µg/mL concentrations respectively, in UT-Med + BT-TI group, while BT-Med + UT-TI group showed increased protection by 68.5%, 70.7%, and 16.8% at 0.1, 1, and 10 µg/mL respectively, and 86.5%, 62.5%, and 34.2% improved cellular protection at 0.1, 1, and 10 µg/mL respectively, in BT-Med + BT-TI group as compared to the untreated test group. The percent protection of HepG2 (liver) cells (decreased of ALT activity) was reported by 33.5%, 63.2%, and 99.2% at 10 µg/mL in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively compared to the untreated group. Cellular protection of A549 (lungs) cells (increased of SOD activity) in terms of percentage was increased by increased by 39.8% (at 10 µg/mL), 44% (at 25.5 µg/mL), and 59.7% (at 25.5 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively compared to untreated group. Serotonin level was significantly increased by 59.2% (at 0.1 µg/mL), 190.3% (at 0.1 µg/mL), and 201% (at 1 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively compared to untreated in human neuroblastoma cells (SH-SY5Y). However, the relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 159.1% (at 50 µg/mL), 212.7% (at 1 µg/mL), and 278.3% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively as compared to the untreated in MG-63 cells. Thus, the present data concluded that the overall multiple organ health using various standard biomarkers in specific cell lines were significantly improved with respect to health of bones, heart, liver, lungs, and brain after treatment with the Biofield Energy treated test formulation (The Trivedi Effect®). Thus, it can be used as a complementary and alternative therapy approach against many multiple organ disorders such as coronary artery disease, arrhythmias, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, asthma, chronic bronchitis, cystic fibrosis, osteoporosis, etc.

scholarly journals Pyrido[2′,1′:2,3]imidazo[4,5-c]isoquinolin-5-amines as Potential Cytotoxic Agents against Human Neuroblastoma

2021 ◽  
Vol 14 (8) ◽  
pp. 750
Author(s):  
Zahira Tber ◽  
Mohammed Loubidi ◽  
Jabrane Jouha ◽  
Ismail Hdoufane ◽  
Mümin Alper Erdogan ◽  
...  
Keyword(s):  
Cell Line ◽  
Drug Exposure ◽  
Caspase 3 ◽  
Biological Activities ◽  
Neuroblastoma Cell ◽  
Neuroblastoma Cell Line ◽  
Cytotoxic Agents ◽  
Human Neuroblastoma Cell ◽  
Human Neuroblastoma ◽  
Parp 1

We report herein the evaluation of various pyrido[2′,1′:2,3]imidazo[4,5-c]isoquinolin-5-amines as potential cytotoxic agents. These molecules were obtained by developing the multicomponent Groebke–Blackburn–Bienaymé reaction to yield various pyrido[2′,1′:2,3]imidazo[4,5-c]quinolines which are isosteres of ellipticine whose biological activities are well established. To evaluate the anticancer potential of these pyrido[2′,1′:2,3]imidazo[4,5-c]isoquinolin-5-amine derivatives in the human neuroblastoma cell line, the cytotoxicity was examined using the WST-1 assay after 72 h drug exposure. A clonogenic assay was used to assess the ability of treated cells to proliferate and form colonies. Protein expressions (Bax, bcl-2, cleaved caspase-3, cleaved PARP-1) were analyzed using Western blotting. The colony number decrease in cells was 50.54%, 37.88% and 27.12% following exposure to compounds 2d, 2g and 4b respectively at 10 μM. We also show that treating the neuroblastoma cell line with these compounds resulted in a significant alteration in caspase-3 and PARP-1 cleavage.

Sign in / Sign up

Export Citation Format

Share Document