scholarly journals Antibacterial action of red and green propolis extract in infected root canal

2017 ◽  
Vol 32 (2) ◽  
pp. 99
Author(s):  
Mônica Misaé Endo ◽  
Cyntia R. A. Estrela ◽  
Ana Helena G. Alencar ◽  
Júlio Almeida Silva ◽  
Daniel Almeida Decurcio ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Antibacterial Action ◽  
Root Canal Preparation ◽  
Passive Ultrasonic Irrigation ◽  
Propolis Extract ◽  
Root Canals ◽  
Infected Root ◽  
Ultrasonic Irrigation ◽  
Conventional Irrigation

OBJECTIVE: This study investigated the antibacterial action of 30% red propolis, 40% green propolis and 2.5% sodium hypochlorite with irrigation protocols in infected root canals.METHODS: During 60 days, twenty-four root canals were inoculated with E. faecalis. In all experimental groups were performed root canal preparation and two irrigation protocols - passive ultrasonic irrigation and conventional irrigation. In the groups 1, 3, 5 and 7 it were made root canal preparation associated to conventional irrigation with 30% propolis, 40% propolis, 2.5% sodium hypochlorite and distilled water, respectively. In groups 2, 4, 6 and 8 it was made root canal preparation associated with passive ultrasonic irrigation with the solutions described above. The groups 9 and 10 were the controls (negative and positive). Samples of the root canals were collected and immersed in 7 mL of BHI, for 48 hours, at 37ºC. Bacterial growth was analyzed by turbidity of the culture medium.RESULTS: Antibacterial action was observed of 30% red propolis and 2.5% sodium hypochlorite when used passive ultrasonic irrigation and conventional irrigation after 20 minutes, but in 72 hours it was not effective.CONCLUSION: The irrigating agents and protocols were not effective to eliminate the microorganisms of the infected root canals. Clinical Significance: Propolis presents potential for clinical application due to its antimicrobial, anti inflammatory, antioxidant and low toxicity properties.

scholarly journals Effect of Passive Ultrasonic Irrigation on Apical Extrusion of Irrigating Solution

2008 ◽  
Vol 02 (03) ◽  
pp. 198-203 ◽  
Author(s):  
Tamer Tasdemir ◽  
Kursat Er ◽  
Davut Celik ◽  
Tahsin Yildirim
Keyword(s):  
Stainless Steel ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Control Group ◽  
Passive Ultrasonic Irrigation ◽  
Root Canals ◽  
Apical Extrusion ◽  
Ultrasonic Irrigation ◽  
The Mean ◽  
Mandibular Incisors

ABSTRACTObjectives: To determine the influence of passive ultrasonic irrigation (PUI) on apical extrusion of irrigating solution.Methods: Twenty freshly extracted maxillary and mandibular incisors with single straight root canals were instrumented with ProTaper rotary files. During final irrigation, the root canals were filled with 2.5% sodium hypochlorite (NaOCl) solution and PUI was performed with a piezoelectronic unit. A stainless steel instrument (size 15) was inserted into the root canal and the irrigant was ultrasonically activated for one minute. This sequence was repeated three times, resultingin a total PUI time of three minutes. The volume of extruded irrigant was measured by Pipetman pipettes. The data were statistically analyzed with the Mann-Whitney U test.Results: The mean volume of apical extruded irrigant was 2.15 �L for the PUI group and 14 �L forthe control group. The experimentalgroup extruded significantly smaller amounts of irrigant than the control group (P<.05).Conclusions: The PUI procedure as a final irrigation was associated with less apical extrusion of the irrigating solution. (Eur J Dent 2008;2:198-203)

Does the Use of Use of Continuous or Passive Ultrasonic Irrigation Protocols Improve the Removal of Smear Layer? A Scanning Electron Microscopic Study

2021 ◽  
Author(s):  
Ramón Miguéns-Vila ◽  
Pablo Castelo-Baz ◽  
Saleta Aboy-Pazos ◽  
David Uroz-Torres ◽  
Pablo Álvarez-Nóvoa ◽  
...  
Keyword(s):  
Negative Pressure ◽  
Root Canal ◽  
Smear Layer ◽  
Electron Microscopic ◽  
Passive Ultrasonic Irrigation ◽  
Root Canals ◽  
Scanning Electron Microscopic Study ◽  
Ultrasonic Irrigation ◽  
Conventional Irrigation ◽  
Scanning Electron

Abstract The aim of this study was to evaluate the effectiveness of smear layer removal after the use of different irrigation methods (passive ultrasonic irrigation [PUI], continuous ultrasonic irrigation [CUI], apical negative pressure irrigation and conventional irrigation) using the scanning electron microscopy (SEM) as an analytical tool. A total of 100 single-canal teeth were decoronated and randomly divided into five groups (n = 20) according to the irrigation method used: conventional irrigation with front outlet syringe, conventional irrigation with lateral outlet syringe, apical negative pressure irrigation (EndoVac), PUI with Irrisafe, and CUI with ProUltra PiezoFlow ultrasonic irrigation needle. Root canal preparation was performed with the ProTaper Gold system up to the F4 instrument and 5.25% NaOCl was used as an irrigant. After chemical-mechanical preparation, the roots were split longitudinally, and the coronal, middle and apical thirds examined. SEM digital photomicrographs were taken at ×1000 magnification to evaluate the amount of smear layer in each root canal third. CUI was more effective in removing the smear layer than the other irrigation protocols. However, none of the irrigation protocols were able to produce root canals completely free from smear layer.

scholarly journals Apically Extruded Debris Using Passive Ultrasonic Irrigation Associated with Different Root Canal Irrigants

2019 ◽  
Vol 30 (4) ◽  
pp. 363-367 ◽  
Author(s):  
Rodrigo Arruda-Vasconcelos ◽  
Marlos Barbosa-Ribeiro ◽  
Lidiane Mendes Louzada ◽  
Giovanna Dornelas Mantovani ◽  
Brenda PFA Gomes
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Saline Solution ◽  
Average Weight ◽  
Passive Ultrasonic Irrigation ◽  
Root Canals ◽  
Root Canal Irrigants ◽  
Ultrasonic Irrigation ◽  
Chlorhexidine Gel ◽  
Chlorhexidine Solution

Abstract The present study evaluated the amount of apically extruded debris after chemo-mechanical preparation (CMP) associated with passive ultrasonic irrigation (PUI) using four different root canal irrigants, namely, 6% sodium hypochlorite (NaOCl), 2% chlorhexidine gel + saline solution (2% CHXg+SS), 2% chlorhexidine solution (2% CHXs) and SS alone. Sixty mandibular premolars with single straight root canals were selected and randomly assigned into 4 groups (n=15) according to the root canal irrigant used as follows: G1 (PUI + NaOCl), G2 (PUI + CHXg+SS), G3 (PUI+CHXs) and G4 (PUI+SS). Reciproc® R25 files (25/.08) were used during CMP and the debris extruded from each tooth were collected in pre-weighted Eppendorf tubes and dried. The average weight of debris was assessed by using an analytical microbalance. Data were statistically analysed by using ANOVA and post-hoc Tukey’s test (a=0.05). Debris extrusion was observed in all groups, irrespective of the root canal irrigating, with 2% CHXg + SS being associated with lower debris extrusion compared to other irrigants (p<0.05). No significant differences were observed between 6% NaOCl, 2% CHXs and SS. In conclusion, passive ultrasonic irrigation did not completely prevent apically extrusion of debris. PUI performed with 2% chlorhexidine gel + saline solution significantly minimized debris extrusion compared to 6% sodium hypochlorite, chlorhexidine solution and saline solution.

scholarly journals Effect of the Passive Ultrasonic Irrigation and the Apical Diameter Size on the Debridement Efficacy of Infected Root Canals: A Multivariate Statistical Assessment of Histological Data

2021 ◽  
Vol 11 (16) ◽  
pp. 7495
Author(s):  
Marcela Alcota ◽  
Jimena Osorio ◽  
Claudia Díaz ◽  
Ana Ortega-Pinto ◽  
Cristián Peñafiel ◽  
...  
Keyword(s):  
Root Canal ◽  
Ordinal Scale ◽  
Multivariate Statistical ◽  
Principal Coordinates Analysis ◽  
Passive Ultrasonic Irrigation ◽  
Root Canals ◽  
Principal Coordinates ◽  
Infected Root ◽  
Histological Data ◽  
Ultrasonic Irrigation

The removal of necrotic and vital pulp substrates and microorganisms and their toxins from the root canal system (RCS) has been found to be the basis for a successful endodontic treatment. In this study, our aim was to evaluate the effect of passive ultrasonic irrigation (PUI) on the elimination of the organic remnant tissue from infected, narrow and curved mandibular root canals during their instrumentation. For this purpose, mesiobuccal canals from mandibular first molars were instrumented with the RaCe rotary system, using PUI activation or conventional irrigation (CI) and two apical diameters (#25 and #35). The root canal cleanness of the samples was evaluated by microscopy and using a modified Langeland’s ordinal scale. Parametric and non-parametric statistical analyses and principal coordinates analysis (PCoA) of the samples were performed. When PUI was used, there was a significant reduction of the organic remnant in the apical enlargement of 25 at 2 mm from the apex (p < 0.001). After pooling the groups, regardless of the depth of the observation (2 and 4 mm from the apex), the pair #35 + PUI vs. #25 + CI showed statistically significant differences (p < 0.001). The effect of PUI explained 65% of the overall variance when compared with the CI samples. The use of PUI reduced the organic material of narrow infected and curved root canals with an apical enlargement of #25 and #35. When PUI is not used, a biomechanical instrumentation up to a diameter ≥#35 is recommended.

scholarly journals Passive ultrasonic irrigation in calcium hydroxide removal from root canals: a sem/eds analysis

2015 ◽  
Vol 3 (3) ◽  
pp. 668
Author(s):  
Fernanda Hoffmann Busanello ◽  
Angela Longo Do Nascimento ◽  
Lilian Tietz ◽  
Mirela Sangoi Barreto ◽  
Ricardo Abreu Da Rosa ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Control Group ◽  
Passive Ultrasonic Irrigation ◽  
Root Canals ◽  
Eds Analysis ◽  
Ultrasonic Irrigation ◽  
Calcium Hydroxide Removal ◽  
And Control ◽  
Elementary Chemical

AIM: The aim of this study was to evaluate the efficacy of different lengths of time of passive ultrasonic irrigation (PUI) in removing calcium hydroxide (CH) paste from root canal, using scanning electron microscopy and energy dispersive spectrometry (SEM/EDS). MATERIAL AND METHODS: Fifty-four human premolars were used. After coronal access, a size #15 K-file was used to obtain apical patency. Root canal preparation was performed using WaveOne 40.08 instruments. CH paste was placed into the root canal. Specimens were stored in a humid environment, and after seven days, they were divided into five groups (n=12) according to the irrigation protocols: Manual– a size #40 K-file; PUI/1– 1 min; PUI/2– 2 min; PUI/3– 3 min; and Control– without CH paste. An elementary chemical microanalysis (SEM/EDS) was performed to quantify the presence of calcium on the dentinal walls. RESULTS: The percentage of calcium was higher in all experimental groups when compared to the control group (P < 0.05); but no differences among them were found (P > 0.05). CONCLUSION: The amount of calcium hydroxide paste on the dentinal walls was not dependent on length of time of ultrasonic activation. SEM/EDS analysis seems to be a reliable method to assess CH paste removal from the root canals.

scholarly journals Effect of a Surfactant on the Antimicrobial Activity of Sodium Hypochlorite Solutions

2014 ◽  
Vol 25 (5) ◽  
pp. 416-419 ◽  
Author(s):  
Marcos Rodolfo Bolfoni ◽  
Marcelo dos Santos Ferla ◽  
Otávio da Silva Sposito ◽  
Luciano Giardino ◽  
Rogério de Castilho Jacinto ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Root Canal Preparation ◽  
Human Teeth ◽  
Significance Level ◽  
Root Canals ◽  
Tukey Test ◽  
Irrigation Solution ◽  
Period Data

The objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (NaOCl) associated with a surfactant. Seventy single-rooted extracted human teeth were inoculated with Enterococcus faecalis, and incubated for 21 days (37 °C). The groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% NaOCl; 5%, 2.5% and 1% Hypoclean(r), a solution containing a surfactant (cetrimide) associated with NaOCl. Three microbiological samples were collected from each tooth: S1 - before instrumentation; S2 - immediately after instrumentation; and S3 - after a seven-day period. Data were submitted to ANOVA and Tukey test with 5% significance level. The results showed that immediately after root canal preparation (S2), E. faecalis was eliminated in all the experimental groups. However, after 7 days (S3), only the groups in which Hypoclean was used, remained contamination-free, including Hypoclean associated with 1% NaOCl, while the root canals irrigated with 1% NaOCl only, presented the highest percentage of bacterial growth. In conclusion, the addition of surfactant increased the antimicrobial activity of 1% NaOCl to levels similar to 5% NaOCl.

scholarly journals Sodium Hypochlorite Irrigation and Its Effect on Bond Strength to Dentin

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Tariq S. Abuhaimed ◽  
Ensanya A. Abou Neel
Keyword(s):  
Bond Strength ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Endodontic Treatment ◽  
Antibacterial Action ◽  
Adhesive Resin ◽  
Root Canals ◽  
Adverse Action ◽  
Complex Anatomy ◽  
Halogenated Compound

Effective shaping and cleaning of root canals are essential for the success of endodontic treatment. Due to the complex anatomy of root canal spaces, the use of various instrumentation techniques alone is not effective in producing bacteria-free root canal spaces. Irrigation, disinfectants, rinses, and intervisit medications are used in conjunction with the mechanical instrumentation to ensure the success of endodontic treatment. Sodium hypochlorite (NaOCl), a halogenated compound, is routinely used to irrigate the root canal during endodontic treatments. NaOCl has been known for its antibacterial action, proteolytic and dissolution capacity, and debridement properties. NaOCl, however, can alter the composition of dentin and hence its interaction with the adhesive resins used to bond the restorative materials to treated dentin. This review therefore covers in depth the action of NaOCl on dentin-adhesive resin bond strength including both enhancement and reduction, then mechanisms proposed for such action, and finally how the adverse action of NaOCl on dentin can be reversed.

scholarly journals Comparison of Antibacterial Effects of Photodynamic Therapy and an Irrigation Activation System on Root Canals Infected With Enterococcus faecalis: An In Vitro Study

2020 ◽  
Vol 11 (3) ◽  
pp. 243-248
Author(s):  
Mohammad Asnaashari ◽  
Niloufar Kooshki ◽  
Mohammad Mahdi Salehi ◽  
Saranaz Azari-Marhabi ◽  
Hedieh Amin Moghadassi
Keyword(s):  
Photodynamic Therapy ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
New Technologies ◽  
Irrigation System ◽  
Bacterial Colony ◽  
Root Canals ◽  
Infected Root ◽  
Activation System

Introduction: Enterococcus faecalis is a resistant bacterium which is the most abundant species in infected root canals. Photodynamic therapy (PDT) is a method for killing the bacteria with active Oxygen radicals generated in a photosensitizer when exposed to centralized light. Furthermore, as a new method of canal disinfection, a variety of irrigation activation systems have been introduced, one of which is GentleFile (GF) with rotary movements and spiral effects for antibacterial action. This study aimed to compare the effectiveness of the two mentioned methods when used with and without Sodium Hypochlorite in eliminating E. faecalis from infected root canals. Methods: Fifty-eight uniradicular teeth were randomly divided into 4 experimental groups of 14. Two specimens were selected for later scanning electron microscopy in order to screen the procedure steps. In each experimental group, 10 samples were selected to be treated with GF or PDT; 3 of them were selected as positive controls and the other one sample was chosen as a negative control. Experimental groups were as follows: (1) Irrigation activation system, (2) Irrigation activation system + sodium hypochlorite, (3) PDT, and (4) PDT+ sodium hypochlorite. The specimens were then cultured for a bacterial colony count. Results: The decrease in the bacterial count after the treatment with the irrigation activation system was 99.8% (P=0.011) and when the system was used with sodium hypochlorite, it was 100% (P=0.001). The antibacterial effect of PDT was 90.08% (P=0.011) and it was 99.7% when PDT was combined with sodium hypochlorite (P=0.011). Conclusion: All four methods can be administered as complementary methods in root canal disinfection. According to the results of disinfection in the experimental groups of current study it is concluded that integration of new technologies such as activation irrigation system or PDT in Combination with NaOCl ameliorates disinfection of root canal and can provide several advantages in the endodontic outcome.

scholarly journals Effect of the irrigation protocols on the elimination of dentin debris from simulated lateral canals

2018 ◽  
Vol 21 (4) ◽  
pp. 437
Author(s):  
Pedro Cesar Gomes Titato ◽  
Lyz Cristina Furquim Canali ◽  
Rodrigo Ricci Vivan ◽  
Marco Antonio Hungaro Duarte
Keyword(s):  
Root Canal ◽  
Ultrasonic Therapy ◽  
Root Canal Therapy ◽  
Passive Ultrasonic Irrigation ◽  
Final Volume ◽  
Continuous Rotation ◽  
Debris Removal ◽  
Computed Microtomography ◽  
Ultrasonic Irrigation ◽  
Conventional Irrigation

<p><strong>Objective: </strong>Computed microtomography was used to evaluate the capacity of debris removal from simulated lateral canals, in conventional irrigation, passive ultrasonic irrigation (PUI), continuous passive irrigation (CUI), Endoactivator and EasyClean, for both types of motion (continuous and reciprocating). <strong>Material and Methods</strong>: Sixty acrylic incisors with 3 simulated lateral canals per tooth (cervical, middle and apical third) were instrumented with Reciproc 40/06 and then filled with dentin debris. The teeth were scanned and divided into 6 groups (n = 10) according to the irrigation protocol: G1 - conventional, G2 - PUI, G3 - CUI, G4 - Endoactivator, G5 - EasyClean continuous rotation and G6 - EasyClean reciprocating. After the irrigation protocols, the samples were scanned again. The initial and final volume images were analyzed and the percentage of debris removal were found for each group in all levels. <strong>Results:</strong> There were significant differences between the initial and final volume of debris after the protocols, with the exception of conventional irrigation group in the apical and cervical thirds, EasyClean reciprocating in the middle and cervical thirds, and CUI in the middle third. In the analysis between the groups: in the apical third, Endoactivator and PUI removed more debris than CUI (p &lt; 0.05); in the middle and cervical thirds, PUI was more efficient than conventional irrigation, EasyClean reciprocating and CUI (p &lt; 0.05). <strong>Conclusion:</strong> No method completely removed the debris. PUI was the method that showed greater effectiveness in cleaning the lateral canals.</p><p><strong>Keywords</strong></p><p>Root canal therapy; Microct; Dentin; Ultrasonic therapy.</p>

scholarly journals Evaluation of Different Passive Ultrasonic Irrigation Protocols on the Removal of Dentinal Debris from Artificial Grooves

2016 ◽  
Vol 27 (5) ◽  
pp. 568-572 ◽  
Author(s):  
Rodrigo Ricci Vivan ◽  
Jussaro Alves Duque ◽  
Murilo Priori Alcalde ◽  
Marcus Vinicius Reis Só ◽  
Clóvis Monteiro Bramante ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Root Apex ◽  
Control Group ◽  
Passive Ultrasonic Irrigation ◽  
Similar Performance ◽  
Ultrasonic Device ◽  
Ultrasonic Irrigation

Abstract The aim of this study was to evaluate the influence of different passive ultrasonic irrigation protocols on the removal of debris in artificially created grooves in the cervical, middle and apical root thirds. Forty extracted bovine incisor roots were instrumented to 1 mm of the root apex with a R50 Reciproc instrument and irrigated with 2.5% sodium hypochlorite. The roots were then inserted in a muffle and cleaved into two hemisections. Grooves (3-mm long) were done at 2, 7 and 12 mm from the root apex in one hemisection and filled with dentinal debris. The hemisections were regrouped into the muffle and divided into 4 groups (n=10) according to the final irrigation protocol: Control group: 3x20 s using a #30 gauge needle without agitation of the irrigating solution; Group PUI-s (static passive ultrasonic irrigation): 3x20 s of passive ultrasonic irrigation with the tip of the insert maintained static on the apical third; Group PUI-t (passive ultrasonic irrigation per third): 20 s of PUI in each third; Group PUI-d (passive dynamic ultrasonic irrigation): 3x20 s of PUI dynamically moving the insert in the whole extent of the root canal. In all groups, was used a total of 6 mL of 2.5% sodium hypochlorite as irrigant. After these procedures, the grooves were analyzed with a stereomicroscope and assigned scores as regards removal of the debris. The data were analyzed statistically (a=0.05). The results showed a better cleaning in all the groups where the irrigating solution was agitated with ultrasonic device than in the control group (p<0.05). In the apical third, the PUI-d and PUI-s showed similar performance (p>0.05) and a better cleanness than PUI-t (p<0.05). The dynamic and static methods of agitation of the irrigating solution provided more effective cleaning. PUI-d provided the most completely clean grooves suggesting that its use is the most adequate in cases of teeth with complex canal anatomy.

Sign in / Sign up

Export Citation Format

Share Document