Bioconjugates of Luminescent CdSe-ZnS Quantum Dots with Engineered Recombinant Proteins: Novel Self-Assembled Tools for Biosensing

2000 ◽  
Vol 642 ◽  
Author(s):  
Ellen R. Goldman ◽  
Hedi Mattoussi ◽  
Phan T. Tran ◽  
George P. Anderson ◽  
J. Matthew Mauro
Keyword(s):  
Quantum Dots ◽  
Recombinant Proteins ◽  
Self Assembly ◽  
Emission Spectra ◽  
Igg Antibody ◽  
Photo Degradation ◽  
Size Dependent ◽  
Near Ir ◽  
Wide Range

ABSTRACTColloidal semiconductor quantum dots (QDs) are luminescent nanoparticles with size- dependent emission spectra spanning a wide range of wavelengths in the visible and near IR. This property, as well as their higher resistance to photo-degradation compared to organic dye labels, makes QDs potentially suitable for certain biomolecule tagging and multiplexing applications. We describe an electrostatic self-assembly approach for conjugating highly luminescent colloidal CdSe-ZnS core-shell QDs with engineered two-domain recombinant proteins to form conjugates for sensing and imaging applications. The design, preparation, and characterization of high quantum yield IgG antibody-binding protein G bioconjugates using luminescence, electrophoretic gel shift, and affinity assays is reported.

scholarly journals Anisometric Ln(III) Complexes with Efficient Near-IR Luminescence

Inorganics ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 9
Author(s):  
Andrey A. Knyazev ◽  
Aleksandr S. Krupin ◽  
Yuriy G. Galyametdinov
Keyword(s):  
Near Infrared ◽  
Emission Spectra ◽  
Structural Features ◽  
Infrared Range ◽  
Light Emitting ◽  
Near Ir ◽  
Re Elements ◽  
Stokes Shifts ◽  
Narrow Emission

Recent studies in development of near-infrared luminophores focus on overcoming their disadvantages such as low quantum efficiency, limited emission power, and broad emission spectra. Rare earth (RE) elements are promising compounds in this respect as they offer a unique set of optical properties that provide narrow emission spectra and large Stokes shifts. This work reports the results of synthesis and characterization of new anisometric complexes of lanthanide(III) tris(b-diketonates) and 1,10-phenanthroline. These complexes possess light emitting-properties in the near-infrared range. Due to their structural features, these complexes allow production of homogeneous films by spin coating. These films are transparent in the visible and near-infrared ranges (transmission up to 99%). This paper demonstrates advantages of Yb(III), Er(III), and Nd(III) complexes as potential components of highly efficient light-transforming NIR coatings.

scholarly journals Self Assembly of semiconductor nanostructures

Respuestas ◽  
2016 ◽  
Vol 12 (2) ◽  
pp. 47-51
Author(s):  
Máximo López-López ◽  
Esteban Cruz-Hernández ◽  
Isaac Martínez-Velis ◽  
Juan Salvador Rojas-Ramírez ◽  
Manolo Ramirez-Lopez ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Molecular Beam Epitaxy ◽  
Self Assembly ◽  
Molecular Beam ◽  
Quantum Wires ◽  
Preferential Growth ◽  
Growth Method ◽  
Strong Surface ◽  
Self Assembled

 Abstract In this work we present the growth and characterization of GaAs self-assembled quantum wires (SAQWRs), and InAs self-assembled quantum dots (SAQDs) by molecular beam epitaxy on (631)-oriented GaAs substrates. Adatoms on the (631) crystal plane present a strong surface diffusion anisotropy which we use to induce preferential growth along one direction to produce SAQWRs. On the other hand, InAs SAQDs were obtained on GaAs(631) with SAQWRs by the Stransky–Krastanov (S-K) growth method. SAQDs grown directly on (631) substrates presented considerable fluctuations in size. We study the effects of growing a stressor layer before the SAQDs formation to reduce these fluctuations.Keywords : Quantum wires, quantum dots; selfassembly; molecular beam epitaxy.

Design and Characterization of Nanoarchitectures from Multifunctional Polyparaphenylenes

2003 ◽  
Vol 776 ◽  
Author(s):  
Renu Ravindranath ◽  
Suresh Valiyaveettil ◽  
Chinnapan Baskar ◽  
Ananda Putra ◽  
Fitri Fitrilawati ◽  
...  
Keyword(s):  
Thin Films ◽  
Conducting Polymers ◽  
Self Assembly ◽  
The Self ◽  
Morphological Properties ◽  
Synthesis And Characterization ◽  
Solution Properties ◽  
Wide Range ◽  
Modified Surfaces

AbstractConducting polymers are interesting materials due to their wide range of applications in electronics, sensing, photonics and display applications. The present paper delineates the synthesis and characterization of the three functionalized poly (p-phenylene)s (PPP) (A-C) and solution properties of the polymers. The self-assembly of the polymers were investigated on various substrates and the optical/morphological properties of thin films of these polymers were also studied. The spontaneous self assembly of the modified PPP's lead to the formation of thin films on both hydrophilic and modified surfaces.

HYBRID SEMICONDUCTING QUANTUM DOTS–METALLIC NANOPARTICLES ARRAYS FOR POSSIBLE NANOPHOTONIC DEVICES

2011 ◽  
Vol 10 (04n05) ◽  
pp. 1113-1118
Author(s):  
M. HARIDAS ◽  
J. K. BASU
Keyword(s):  
Quantum Dots ◽  
Self Assembly ◽  
Metallic Nanoparticles ◽  
Quantum Information Processing ◽  
Imaging Techniques ◽  
Large Area ◽  
Nanophotonic Devices ◽  
Wide Range ◽  
Potential Applications ◽  
Assembly Technique

Arrays of quantum dots and hybrid arrays of semiconducting quantum dots and metallic nanoparticles have wide range of potential applications from nanophotonics to quantum information processing. Creating such arrays with well-defined morphology and order over a large area is a challenge. We present a reliable method for constructing such arrays using simple self assembly technique. The reliability of the method is verified using AFM. The emission properties of such system are studied using high resolution imaging techniques and we have given the possible explanation for the observed phenomena.

scholarly journals Specific Visualization of Tumor Cells Using Upconversion Nanophosphors

Acta Naturae ◽  
2014 ◽  
Vol 6 (4) ◽  
pp. 48-53 ◽  
Author(s):  
E. A. Grebenik ◽  
A. N. Generalova ◽  
A. V. Nechaev ◽  
E. V. Khaydukov ◽  
K. E. Mironova ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Self Assembly ◽  
Malignant Tumors ◽  
Emission Spectra ◽  
Biological Tissues ◽  
Medical Diagnostics ◽  
Breast Adenocarcinoma ◽  
Wide Range ◽  
Two Component ◽  
Human Malignant Tumors

The development of targeted constructs on the basis of photoluminescent nanoparticles with a high photo- and chemical stability and absorption/emission spectra in the transparency window of biological tissues is an important focus area of present-day medical diagnostics. In this work, a targeted two-component construct on the basis of upconversion nanophosphors (UCNPs) and anti-tumor 4D5 scFv was developed for selective labeling of tumor cells overexpressing the HER2 tumor marker characteristic of a number of human malignant tumors. A high affinity barnase : barstar (Bn : Bs) protein pair, which exhibits high stability in a wide range of pH and temperatures, was exploited as a molecular adapter providing self-assembly of the two-component construct. High selectivity for the binding of the two-component 4D5 scFv-Bn : UCNP-Bs construct to human breast adenocarcinoma SK-BR-3 cells overexpressing HER2 was demonstrated. This approach provides an opportunity to produce similar constructs for the visualization of different specific markers in pathogenic tissues, including malignant tumors.

Characterization of Chaotic Motion of DNA in Linear Shear Flows

2003 ◽  
Author(s):  
D. G. Walker ◽  
M. A. Stremler
Keyword(s):  
Self Assembly ◽  
Dna Hybridization ◽  
Shear Flows ◽  
Coarse Grained ◽  
Stable Limit ◽  
Flow Conditions ◽  
Wide Range ◽  
Linear Shear ◽  
Stable Limit Cycles

Motion of macromolecules in flows is important to several disciplines such as DNA hybridization studies, self assembly of nanostructures, and transport of suspensions. The present study simulates the motion of macromolecular structures in linear shear flows. A molecular chain is modeled as a coarse-grained series of beads and springs. For a wide range flow conditions, the flow appears chaotic, where quasi-stable limit cycles are observed for several smaller ranges of flow conditions.

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