scholarly journals PENERAPAN LEMBAR KERJA BERBASIS INKUIRI PADA ANALISIS METABOLIT SEKUNDER BEBERAPA TANAMAN OBAT INDONESIAt

2021 ◽  
Vol 6 (2) ◽  
pp. 167-173
Author(s):  
Shoyang Gadis Supratania ◽  
Saepudin Rahmatullah ◽  
Neneng Windayani
Keyword(s):  
Organic Chemistry ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Natural Product ◽  
Secondary Metabolite ◽  
Process Skills ◽  
Metabolite Analysis ◽  
Average Value ◽  
Design Experiments

Secondary metabolites are part of the discussion of Natural Product Organic Chemistry which demands a practicum process in lectures. This research aims to determine process skills through the application of inquiry-based worksheets on secondary metabolite analysis of three types of Indonesian medicinal plants. In the worksheet, there were several expected goals, including developing students' abilities in designing experiments, conducting experiments, and communicating both orally and in writing. The method used in this research was a One-Shot Case Study with 18 students taking Natural Product Organic Chemistry courses. The instruments used were learning descriptions, inquiry-based worksheets, observation sheets, assessment sheets (psychomotor, presentations, and reports). The results of the worksheet application showed that the students' ability obtain an average value of 84.15 with a very good category. The ability to design experiments, conduct experiments, and communicate orally and in writing obtained results of 77.16 (good categories), 92.50 (very good), and 82.80 (very good), respectively. This inquiry-based worksheet can be used in the study of Natural Product Organic Chemistry, especially in secondary metabolites.

scholarly journals Cleaning the Cellular Factory–Deletion of McrA in Aspergillus oryzae NSAR1 and the Generation of a Novel Kojic Acid Deficient Strain for Cleaner Heterologous Production of Secondary Metabolites

2021 ◽  
Vol 2 ◽  
Author(s):  
Trong T. Dao ◽  
Kate M. J. de Mattos-Shipley ◽  
Ian M. Prosser ◽  
Katherine Williams ◽  
Marija K. Zacharova ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Natural Product ◽  
Secondary Metabolite ◽  
Aspergillus Oryzae ◽  
Kojic Acid ◽  
Negative Regulator ◽  
Secondary Metabolite Production ◽  
Phenotypic Change ◽  
Metabolite Production ◽  
Host Strains

The use of filamentous fungi as cellular factories, where natural product pathways can be refactored and expressed in a host strain, continues to aid the field of natural product discovery. Much work has been done to develop host strains which are genetically tractable, and for which there are multiple selectable markers and controllable expression systems. To fully exploit these strains, it is beneficial to understand their natural metabolic capabilities, as such knowledge can rule out host metabolites from analysis of transgenic lines and highlight any potential interplay between endogenous and exogenous pathways. Additionally, once identified, the deletion of secondary metabolite pathways from host strains can simplify the detection and purification of heterologous compounds. To this end, secondary metabolite production in Aspergillus oryzae strain NSAR1 has been investigated via the deletion of the newly discovered negative regulator of secondary metabolism, mcrA (multicluster regulator A). In all ascomycetes previously studied mcrA deletion led to an increase in secondary metabolite production. Surprisingly, the only detectable phenotypic change in NSAR1 was a doubling in the yields of kojic acid, with no novel secondary metabolites produced. This supports the previous claim that secondary metabolite production has been repressed in A. oryzae and demonstrates that such repression is not McrA-mediated. Strain NSAR1 was then modified by employing CRISPR-Cas9 technology to disrupt the production of kojic acid, generating the novel strain NSARΔK, which combines the various beneficial traits of NSAR1 with a uniquely clean secondary metabolite background.

scholarly journals PERANAN MIKROBA ENDOFIT UNTUK PENGEMBANGAN OBAT ANTI KANKER

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Siti Rofida
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Anticancer Activity ◽  
Natural Product ◽  
Host Plant ◽  
Anticancer Drugs ◽  
Endophytic Fungi

Natural product that has developed as anticancer drugs are consists from secondary metabolites that has isolated from plant, ocean plant and microorganisms. To maintain its productivity, must be concerned to the cultivation of the medicinal plants. Medicinal plants need time to grow and producing secondary metabolites that would be isolated, so that the production of secondary metabolites compound from plant is very limited.             The plants that infected by endophytic fungi be able to grow faster than the normal plants. Endophytic fungi also have ability producing secondary metabolites compound that produced by the host plant. Taxol, podophyllotoxin and camptothecin are compounds that proved has anticancer activity. Endophytic fungi have also proved increasing the production of the compound.

Secondary Metabolites from the Marine Alga Caulerpa brownii (Chlorophyta)

2005 ◽  
Vol 58 (1) ◽  
pp. 39 ◽  
Author(s):  
Jackie T. Handley ◽  
Adrian J. Blackman
Keyword(s):  
Secondary Metabolites ◽  
Natural Product ◽  
Secondary Metabolite ◽  
Marine Alga ◽  
The Novel ◽  
Green Seaweed ◽  
First Time ◽  
Terpenoid Esters

The green seaweed Caulerpa brownii (Chlorophyta, Caulerpaceae) occurs in Tasmania in two morphological forms (branched and unbranched) and each form has a different profile of diterpenoid secondary metabolites. Unbranched specimens gave rise to the novel secondary metabolites 11, 13, 14, 17, and 18, the secondary metabolite 8 that has been isolated for the first time as a natural product, as well as the known compounds 1 and 3–7. Branched specimens of C. brownii yielded the novel terpenoid esters 21 and the known compounds 1 and 2.

scholarly journals Diversity and Conservation through Cultivation of Hypoxis in Africa—A Case Study of Hypoxis hemerocallidea

Diversity ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 122 ◽  
Author(s):  
Motiki M. Mofokeng ◽  
Hintsa T. Araya ◽  
Stephen O. Amoo ◽  
David Sehlola ◽  
Christian P. du Plooy ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Drug Development ◽  
Natural Product ◽  
Southern Africa ◽  
Endemic Species ◽  
Current Knowledge ◽  
Strong Case ◽  
Wild Harvesting ◽  
Hypoxis Hemerocallidea

Africa has the largest diversity of the genus Hypoxis, accounting for 61% of the current globally accepted taxa within the genus, including some endemic species. Using Hypoxis hemerocallidea as a case study, this review addresses the conservation concerns arising from the unsustainable, wild harvesting of a number of Hypoxis species. Hypoxis hemerocallidea is one of the wild-harvested, economically important, indigenous medicinal plants of southern Africa, with potential in natural product and drug development. There are several products made from the species, including capsules, tinctures, tonics and creams that are available in the market. The use of H. hemerocallidea as a “cure-all” medicine puts an important harvesting pressure on the species. Unsustainable harvesting causes a continuing decline of its populations and it is therefore of high priority for conservation, including a strong case to cultivate the species. Reviewing the current knowledge and gaps on cultivation of H. hemerocallidea, we suggest the creation of a platform for linking all the stakeholders in the industry.

scholarly journals Pengaruh Lembar Kerja Peserta Didik Berbasis Collaborative Teamwork Learning Terhadap Hasil Belajar [The Effect of Collaborative Teamwork Learning Based Students on Learning Outcomes]

2019 ◽  
Vol 8 (2) ◽  
pp. 171
Author(s):  
Dwi Siti Sholeha ◽  
Agus Suyatna ◽  
Kartini Herlina
Keyword(s):  
Student Learning ◽  
T Test ◽  
Process Skills ◽  
Learning Achievement ◽  
Science Process Skills ◽  
Paired Sample ◽  
Average Value ◽  
Collaboration Skills ◽  
The One

This study aims to determine the effect of using student worksheets based on collaborative teamwork learning on student learning achievement. The research design used was One Shot Case Study and One Group Pretest Posttest Design. The research data was taken from the value of observations and initial and final tests whose results were tested using the One Sample T-Test and Paired Sample T-Test. Based on the results of hypothesis testing using one sample t-test with Asymp.Sig. (2-tailed) values obtained ≤ 0.05 so H0 is rejected, which means the average value of science process skills and collaboration skills> 72, the results of paired sample t-test the value of pretest-posttest cognitive ability has the same Sig. (2-tailed) value and below 0.05 is 0,000 so H1 is accepted, meaning that there are differences in the average pretest-posttest results using LKPD based on collaborative teamwork learning so that there is an influence of student worksheets based on collaborative teamwork learning on student learning achievement as indicated by the average value of collaboration skills and science process skills> 72.

scholarly journals Orthosiphon stamineus Benth (Uses and Bioactivities)

2019 ◽  
Vol 3 (1) ◽  
pp. 26
Author(s):  
Marina Silalahi
Keyword(s):  
Diabetes Mellitus ◽  
Medicinal Plants ◽  
Secondary Metabolites ◽  
Organic Acid ◽  
Traditional Medicine ◽  
Secondary Metabolite ◽  
Online Media ◽  
Scientific Journals ◽  
Orthosiphon Stamineus

<p><em>Orthosiphon stamineus</em> Benth., or <em>kumis kucing</em> is a the medicinal plants have been used as diuretic medicine. The utilization of medicinal plants associated to its secondary metabolite. This article aims to explain uses pf the <em>O. stamineus</em> and the its secondary metabolites. This article is based on literature offline and online media. Offline literature used the books, whereas online media used Web, Scopus, Pubmed, and scientific journals. <em>Orthosiphon stamineus</em> has two varieties called purple varieties (which have purple-colored flowers) and white varieties (which have white-colored flowers). Some of secondary metabolites in the <em>O. stamineus </em>are the terpenoids, phenols, isopimaran type ispenimoids, flavonoids, benzochromes, and organic acid derivatives. The traditional medicine of the <em>Orthosiphon stamineus</em> uses as the diuretic, hypertension, hepatitis, jaundice, and diabetes mellitus.</p><p> <em></em></p><p><strong>Keywords</strong>: <em>Orthosiphon stamineu</em><em>s</em><em>, diuretics, diterpenoids, hypertension</em></p><p> </p>

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