scholarly journals REPRODUCTIVE PERFORMANCE OF DOMESTICATED BROODSTOCK OF SILVER PERCH, Bidyanus bidyanus (MITCHELL 1838) AND THE RELATIONSHIP BETWEEN OIL GLOBULE FRAGMENTATION AND EGG QUALITY

2017 ◽  
Vol 12 (2) ◽  
pp. 43 ◽  
Author(s):  
Sulaeman Sulaeman ◽  
Ravi Fotedar
Keyword(s):  
Survival Rate ◽  
Reproductive Performance ◽  
Egg Quality ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
High Fecundity ◽  
Fertilisation Rate ◽  
Significant Difference ◽  
The Relationship

The experiments investigated the reproductive performance of the domesticated broodstock of the silver perch and the relationship between various degrees of oil globule fragmentation and egg quality. Six years old of second generation broodstock (n=3) were evaluated based on the fecundity, fertilisation rate, hatching rate, the degree of oil fragmentation of egg, and the quality of embryos and larvae produced. The fragmentation were grouped into three categories: un-fragmented (cat-1), moderately fragmented (cat-2), and highly fragmented (cat-3). The results showed that the broodstock had a relatively high fecundity (132,400 ± 7,22), fertilization rate (94.27 ± 1.28%), and hatching rates (87.94 ± 1.23%). The survival rate of larvae at 12 days post hatching (dph) in cat-1 (71.3 ± 0.9%) was higher than cat-2 (66.7 ± 0.9%) whereas cat-2 was higher than cat-3 (61.3 ± 0.3%). The eggs was dominated by cat-1 (78.11 ± 2.44%) which was significantly higher than cat-2 (21.26 ± 2.45%) and cat-3 ones (0.40 ± 0.21%). The survival rate of embryo at 20 hours post spawning (hps) and hatching rate of cat-1 (95.33 ± 0.00% and 93.33 ± 0.00%) and cat-2 (90.00 ± 0.00% and 85.00 ± 0.00%) were significantly higher than cat-3 (72.33 ± 1.76% and 60.33 ± 0.00%). The total length (TL) of the larvae of cat-1 and cat-2 (8.44 ± 0.21 mm and 8.35 ± 0.23 mm respectively) were significantly higher than larvae of cat-3 (7.09 ± 0.14 mm). No significant difference was found in the larval deformities among any categories. In conclusion, the reproductive performance of six year-old broodstock silver perch showing acceptable performance and egg categorisation based on oil globule fragmentation can be used as a useful tool to indicate eggs quality of silver perch.

scholarly journals Reproductive Performances and Egg Qualities in African Catfish (Clarias gariepinus) Broodstocks Supplemented with Curcumin and Thyroxine Hormone

Omni-Akuatika ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 32
Author(s):  
Livana Dethris Rawung ◽  
Damiana Rita Ekastuti ◽  
Muhammad Zairin Junior ◽  
Min Rahminiwati ◽  
Ade Sunarma ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Egg Quality ◽  
Gonadosomatic Index ◽  
Clarias Gariepinus ◽  
Density Lipoprotein ◽  
Cholesterol Concentration ◽  
Hepatosomatic Index ◽  
African Catfish ◽  
Hatching Rate ◽  
Significant Difference

An experiment was designed to study the effects of curcumin and thyroxine hormone supplementation in the diet to improve the reproductive performance of catfish broodstock by improving egg quality that eventually increases the production of seedlings. Catfish used in this experiment were supplemented with curcumin and thyroxine hormone through their feeds for 12 weeks. The results showed that there was no significant difference (p > 0.05) in the hepatosomatic index (HSI), gonadosomatic index (GSI), percentage of gonad maturity, total cholesterol concentration in the spawned eggs, high density lipoprotein (HDL) concentration in the spawned eggs, fertilization rate of spawned eggs, and hatching rate of fertilized eggs.  However, there were significant differences (p < 0.05) in the concentration of vitellogenin in the spawned eggs, egg diameter of the spawned eggs, and the triglycerides contents of spawned eggs. It was concluded that curcumin and thyroxine supplementations of African catfish increased vitellogenin concentrations and diameters of spawned eggs that have great potential to improve the reproductive performance.

scholarly journals PERFORMA REPRODUKSI UDANG WINDU, Penaeus monodon TRANSGENIK PASCA INSEMINASI BUATAN MENGGUNAKAN SUMBER SPERMATOFOR YANG BERBEDA

2018 ◽  
Vol 13 (1) ◽  
pp. 11 ◽  
Author(s):  
Samuel Lante ◽  
Andi Tenriulo ◽  
Andi Parenrengi
Keyword(s):  
Reproductive Performance ◽  
Penaeus Monodon ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
Air Exposure ◽  
Wild Male ◽  
Tiger Shrimp ◽  
Larval Quality ◽  
Significant Difference ◽  
Different Sources

Udang windu transgenik merupakan udang hasil rekayasa dengan mengintroduksikan gen antivirus yang diisolasi dari udang windu untuk menghasilkan fenotipe yang lebih baik. Domestikasi udang transgenik telah dilakukan dan berhasil memijah/bertelur, tetapi umumnya telurnya infertil yang disebabkan tidak terjadinya pembuahan di tambak pemeliharaan. Udang betina tidak kawin ditandai tidak membawa spermatofor di telikumnya. Upaya untuk mendapatkan telur fertil udang dengan inseminasi buatan (IB) perlu dilakukan. Tujuan penelitian untuk mengevaluasi performa reproduksi udang betina transgenik dan mutu larva yang dihasilkan pasca IB menggunakan sumber spermatofor yang berbeda. Penelitian ini dirancang dengan tiga perlakuan yaitu: IB menggunakan spermatofor udang windu jantan transgenik (SJT), spermatofor udang windu jantan alam Sulawesi Selatan (SulSel) (SJS) dan spermatofor udang windu jantan alam Aceh (SJA). IB dilakukan pada udang windu betina transgenik setelah dua hari moulting. Hasil penelitian menunjukkan bahwa udang windu betina transgenik pasca IB perlakuan SJT menghasilkan total telur fertil sebanyak 766.949 butir, perlakuan SJS 535.644 butir dan perlakuan SJA 678.016 butir dengan daya tetas telur fertil yaitu: pada SJT, SJS, dan SJA masing-masing adalah 53,5%; 53,7%; dan 55,0%. Uji vitalitas larva dengan perendaman dalam larutan formalin 150-200 mg/L, perendaman air tawar: 5-15 menit, dan pengeringan 3-9 menit menghasilkan sintasan larva udang yang relatif sama pada ketiga perlakuan. Nilai morfologi larva perlakuan SJT, SJA, dan SJS adalah masing-masing 85,0; 84,5; dan 75,0. Dari hasil penelitian ini mengindikasikan bahwa performa reproduksi udang windu betina transgenik dan mutu larva yang dihasilkan pasca IB tidak dipengaruhi oleh sumber spermatofor induk udang windu jantan Penaeus monodon.Transgenic tiger shrimp, Penaeus monodon has been developed in the last decade to equip shrimp with immunity against viral diseases. However, the effort to produce large quantities of specific pathogen resistance (SPR) tiger shrimp seed is hampered by several constraints in the domestication process. The successfulness of domesticated broodstock in producing larvae is very low due to low fertilization rate. An artificial insemination (AI) offers a solution to increase fertilization rate in crustacean. This study was aimed to evaluate the reproductive performance of female transgenic tiger shrimp broodstock and their larval quality after artificially inseminated with males from different sources. The spermatophores of male from different sources i.e. transgenic male spermatophore (SJT), wild male from South Sulawesi (SJS), and wild male from Aceh (SJA) were collected through electric shock and inseminated to female transgenic broodstock two days after moulting. The results showed that the total numbers of fertile eggs produced from SJT, SJS, and SJA treatment were 766,949 pcs; 535,644 pcs; and 678,016 pcs, respectively and not significantly different (P>0.05). Similar to the number of fertile eggs, the hatching rate of eggs of SJT (53.5%), SJS (53.7%), and SJA (55.0%) also did not indicate any significant differences (P>0.05). On the larval vitality test by soaking the larvae in formalin and freshwater as well as by air exposure at different duration showed no significant difference on the survival rate (P>0.05) as indicated by score value at each treatment of 85.0, 84.5, and 75.0 for SJT, SJS, and SJA, respectively. In conclusion, the reproductive performance of female transgenic tiger shrimp and their larval quality were not affected by the different sources of spermatophores inseminated artificially during the spawning cycle.

scholarly journals Effect of dietary astaxanthin on reproductive performance, egg quality and larvae of clowfish Amphiprion ocellaris (Cuvier, 1830)

2021 ◽  
Vol 20 (4A) ◽  
pp. 163-172
Author(s):  
Nguyen Thi Nguyet Hue ◽  
Ho Son Lam ◽  
Dao Thi Hong Ngoc ◽  
Dang Tran Tu Tram ◽  
Huynh Minh Sang ◽  
...  
Keyword(s):  
Survival Rate ◽  
Reproductive Performance ◽  
Egg Quality ◽  
Quality Parameters ◽  
Hatching Rate ◽  
Larval Size ◽  
Spawning Period ◽  
Spawning Frequency ◽  
Feeding Trials ◽  
Egg Diameter

This study was designed to evaluate the effects of astaxanthin in broodfish diets on reproductive performance, egg quality and larvae quality parameters of clownfish (Amphirion ocellaris). Five treatments were tested with 5 levels of astaxanthin (Carophyll Pink 10% CWS) of 0, 50, 100, 150 and 200 mg/kg added to the feed. Each treatment was repeated in triplicate and the supplemental feeding trials were arranged for 13 months. The results showed that there were significant differences in hatching rate of egg, malformed rate and survival rate of larvae in 3 days post-hatch (p<0.05) among the feeding trials of astaxanthin supplements. The highest hatching rate of egg and survival rate and the lowest malformed rate of larvae were observed in the treatment that was supplemented with astaxanthin 150 mg/kg feed, respectively 92.14 %; 93.57 % and 0.55 %. However, the astaxanthin supplementary diets did not affect the re-maturation and spawning period, spawning frequency, fecundity, egg diameter and larval size of nemo fish among the treatments. The results also suggested that astaxanthin requirement for clownfish broodstock to improve reproductive performance was 150 mg/kg feed.

scholarly journals Comparative Test Of Ovaprim, Spawnprim, And Hcg In The Process Of Spawning Of Pangasianodon Hypopthalmus, Sauvage 1987.

2021 ◽  
Vol 26 (1) ◽  
pp. 17
Author(s):  
Vinka Leonita ◽  
Deny Sapto Chondro Utomo ◽  
Hilma Putri Fidyandini
Keyword(s):  
Reproductive Performance ◽  
Design Method ◽  
Economic Value ◽  
Fertilization Rate ◽  
Comparative Test ◽  
Hatching Rate ◽  
Hormonal Stimulation ◽  
Pangasianodon Hypophthalmus ◽  
Significant Difference ◽  
Production Target

Pangasianodon hypophthalmus is a type of freshwater fish that has potential and has economic value. Its production target which increases every year causes farmers to spawn with the help of hormonal stimulation. The aimed of this research was to test and find out the results of the use of three different hormones namely Ovaprim, Spawnprim, and HCG in P. hypopthalmus spawning. This research was conducted in January 2020 at the Trimurjo Fish Seed Center, Central Lampung. This research used a randomized complete design method with the 3 treatments, P1 (Ovaprim 0,5 ml / kg), P2 (Spawnprim 0,5 ml / kg), P3 (HCG 500 IU / kg) and 3 replications. The results of this research indicate a significant difference (p <0,05) in the relative fecundity parameters, fertilization rate, and hatching rate, but not significantly different in the parameters of latensi periode and diameter of eggs. In P1, Ovaprim hormone injections provide better reproductive performance compared to Spawnprim and HCG injections.

scholarly journals Comparison of reproductive performance of Clarias batrachus (Linnaeus, 1758) collected from three Indian rivers

2016 ◽  
Vol 63 (3) ◽  
Author(s):  
Ravi Charan ◽  
Kiran Dube ◽  
P. P. Suresh Babu ◽  
S. Dam Roy ◽  
Rupam Sharma ◽  
...  
Keyword(s):  
Weight Gain ◽  
Survival Rate ◽  
Reproductive Performance ◽  
Andhra Pradesh ◽  
Clarias Batrachus ◽  
Hatching Rate ◽  
Percentage Weight Gain ◽  
Fertilisation Rate ◽  
Percentage Weight ◽  
Somatic Index

A study was conducted to compare the reproductive performance of Clarias batrachus (Linnaeus, 1758) collected from three different rivers viz., Krishna in Andhra Pradesh, Godavari in Maharashtra and Narmada in Madhya Pradesh. Twenty pairs of brooders from each location were randomly selected and induced with pituitary extract (P) and ovaprim (O). Reproductive performance in terms of stripping percentage, pseudo gonado-somatic index, spawning, fecundity, fertilisation rate, hatching rate  and survival rate as well as fry rearing performance in terms of percentage weight gain, specific growth rate (SGR) and survival rate were monitored. Brooders collected from Godavari exhibited significantly (p<0.05) higher values for all the reproductive performances indicators compared to brooders collected from Narmada and Krishna rivers. But fishes collected from Krishna River exhibited significantly higher (p<0.05) fry survival. Fry produced from Godavari brooders attained highest (p<0.05) percentage weight gain (1085.88±37.41), SGR (17.61±0.19) and fry survival rate (43.67±1.45) in comparison to Narmada. Results clearly indicated that reproductive performance varied significantly among the brooders collected from the three different rivers.

scholarly journals Reproductive broodstock performance and egg quality of wild-caught and first-generation domesticated Seriola rivoliana reared under same culture conditions

2017 ◽  
Vol 43 (5) ◽  
pp. 953-962
Author(s):  
Marcos F. Quiñones-Arreola ◽  
G. Fabiola Arcos-Ortega ◽  
Vicente Gracia-López ◽  
Ramón Casillas-Hernández ◽  
Charles Weirich ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Biochemical Composition ◽  
Egg Quality ◽  
Research Work ◽  
Fertilization Rate ◽  
Culture Conditions ◽  
Adult Size ◽  
Spawning Frequency ◽  
Egg Diameter

Almaco jack, Seriola rivoliana as well as some related species is of great interest in marine fish aquaculture. However, there are few studies about their reproduction in captivity. In this research work, reproductive performance and egg quality in two groups of adult Seriola rivoliana, caught in the wild and domesticated-F1 analyzed and compared, reared under optimal maturation conditions in a commercial private Laboratory. A total of 28 wild adult (>5 kg) were caught at La Paz Bay, Baja California Sur, Mexico, and 30 adult domesticated-F1 broodstock (>5 kg), were obtained from an original stock of 1,000 juveniles (3.5 g body weight) produced at Kona Blue (Hawaii, USA) sea farm. Fishes were transported to the Rancheros del Mar commercial private hatchery, where they were grown to adult size. Both groups were evaluated during eight months (May to December 2012) and compared in terms of reproduction performance (total number of spawning events, monthly spawning frequency, total number of eggs, total number of eggs per mL, and fertilization rate), egg biochemical composition (total proteins, total lipids, total carbohydrates, and triacylglycerides) and egg diameter. Results indicated that wild caught broostock showed a better reproductive performance in terms of fertilization rate, total number of spawning, monthly spawning frequency and total number of eggs produced. However, biochemical composition and egg diameter did not show statistical differences (P < 0.05) between two groups. The reproductive performance of broodstock and quality of eggs analyzed in this study are important traits to improve the aquaculture management of this species.

122 THE SURVIVAL RATE AND EMBRYONIC QUALITY OF BOVINE PARTHENOGENETIC BLASTOCYSTS POST-VITRIFICATION CRYOPRESERVATION

2010 ◽  
Vol 22 (1) ◽  
pp. 220
Author(s):  
P. Zhang ◽  
Z. W. Wang ◽  
B. Tang ◽  
J. B. Zhang ◽  
Z. Y. Li
Keyword(s):  
Survival Rate ◽  
Sucrose Solution ◽  
Polar Body ◽  
Cell Counting ◽  
Differential Staining ◽  
Hatching Rate ◽  
First Polar Body ◽  
Significant Difference

Cryopreservation of oocytes and embryos is very useful to conservation of animal genetic resources. Recently, parthenogenesis has received considerable attention as a tool for the production of stem cells. Oocytes and embryos undergo considerable morphological changes and functional damage during cryopreservation, and the survival rate is highly depending on species and developmental stage of oocytes and embryos. The aim of this study was to investigate the survival rate and embryonic quality of bovine parthenogenetic blastocysts post-vitrification cryopreservation. Cumulus-oocyte complexes (COC) from slaughterhouse ovaries were aspirated from 2-mm to 8-mm visible follicles with a 5-mL syringe. The COC were matured in vitro for 22 h in bicarbonate-buffered TCM199 media supplemented with 1 mg mL-1 of FSH, 10 mg mL-1 of LH, 1 mg mL-1 of 17-βiestradiol, and 10% FBS. After in vitro maturation, cumulus cells were removed from COC, oocytes with first polar body were activated by 5 μM ionomycin for 5 min and 2 mM 6-DMAP for 4 h. Subsequently, oocytes were co-cultured with bovine fetal fibroblast cells in SOF media supplemented with amino acids (1% NEAA and 2% EAA), 4 mg mL-1 of BSA, and 10% FBS at conditions of 38.5°C and 5% CO2 for 7 to 9 days. The good expanded blastocysts were selected and refrigerated in different vectors [glass micropipettes (GMP) and straws] and same vitrification solution (VS, 20% EG + 20% DMSO). Blastocysts were exposed to VS, loaded on vectors, and plunged into liquid nitrogen within 25 s. After two days refrigeration, vitrified blastocysts were thawed in air for 10 s and placed into 0.25 M sucrose solution for 1 min and 0.15 M sucrose solution for 5 min. Then, the blastocysts were cultured in the SOF medium same as above. Our results showed that when VS was 20% EG + 20% DMSO, the hatching rate (65%) of blastocysts loaded into GMP was significantly higher (P < 0.05) than that (19%) of blastocysts loaded into straws post-vitrification. Meanwhile, vitrified and nonvitrified blastocysts were fixed and stained for differential cell counting as described by Thouas GA et al. 2001 Reprod. Biomed. Online 3, 25-29). By the differential staining, the total cells of nonvitrified parthenogenetic bovine blastocysts were 102.7, which was higher (P > 0.05) than that (86.7) of vitrified blastocysts. Also, no significant difference (P > 0.05) was seen on ratios between vitrified blastocysts (ICM/TE = 0.22) and nonvitrified blastocysts (ICM/TE = 0.25). Our results indicated that a glass micropipette vector was much better than a straw in vitrification cryopreservation of bovine parthenogenetic blastocysts and caused less damage to blastocyst cells. This study lays the foundation for further research to increase the survival rate of vitrification cryopreservation of bovine embryos. This work was supported by the grant from national support plan, China, No. 2007BAD55B03; corresponding author: Ziyi Li.

scholarly journals Effect of rearing density on reproduction and embryogenesis of zebrafish Danio rerio (Hamilton, 1922)

2017 ◽  
Vol 26 (1) ◽  
pp. 77-82
Author(s):  
Md Golam Rabbane ◽  
Md Mizanur Rahman ◽  
Md Alamgir Kabir ◽  
Md Hasan Faruque
Keyword(s):  
Survival Rate ◽  
Danio Rerio ◽  
Fertilization Rate ◽  
Hatching Rate ◽  
Significant Difference ◽  
Rearing Density ◽  
Spawning Success

Effect of rearing density (5, 15, 25, 35 and 45 fish/l2) on reproductive performances such as spawning success, fertilization rate, hatching rate and embryogenesis in terms of per cent survival rate of embryos at cleavage, gastrula, segmentation, pharyngula and hatching were evaluated. Survival, hatching, fertilization rate and per cent survivability of embryos did not differ significantly between the treatments 1, 2 and 3, but showed significant difference (p < 0.05) between 4 and 5. Treatment three (25 fish/l2) had five-folds higher number of successful progeny than treatment one. Results suggest that 25 fish/l2 can be used as optimum rearing density. Dhaka Univ. J. Biol. Sci. 26(1): 77-82, 2017 (January)

scholarly journals SPAWNING OF BLACK GHOST KNIFEFISH, Apteronotus albifrons WITH DIFFERENT SEX RATIOS

2021 ◽  
Vol 16 (1) ◽  
pp. 29
Author(s):  
Fajar Maulana ◽  
Dinar Tri Soelistyowati ◽  
Muhammad Fadlan Furqon
Keyword(s):  
Reproductive Performance ◽  
Sex Ratios ◽  
Fertilization Rate ◽  
Female Competition ◽  
Hatching Rate ◽  
Spawning Behavior ◽  
Randomized Design ◽  
Study Results ◽  
Significant Difference ◽  
Fish Spawning

Black ghost knifefish, Apteronotus albifrons, is a South America-introduced ornamental fish species that has been widely cultivated in Indonesia. Some farmers breed this fish with different sex ratios, but the optimum sex ratio remains unclear. This study aimed to evaluate the spawning behavior and reproductive performance of black ghost knifefish with different sex ratios. The treatments in this study were arranged in a completely randomized design consisting of different sex ratios between males and females, namely: A (one male : three females), B (two males : three females), and C (three males : three females). Each treatment was done in triplicate. The broodstock were maintained in an aquarium (80 cm x 40 cm x 40 cm) and fed with bloodworm twice a day. The water was changed every day as much as 60% of the total volume. During the experiment, the parameters of spawning behavior, number of fish spawning, number of eggs, fertilization rate, hatching rate, and daily spawning frequencies were observed. The observation was done for seven days. The study results showed that black ghost knifefish spawned at night (11 pm - 2 am). The mating and spawning occurred between one male and one female. Competition between males was observed in the treatments indicated by aggressive movements of a male toward the others, such as sudden approaching, chasing, and driving away the others. Fish in treatment-B spawned consistently from day-1 to day-5. Fish in treatment-A spawned from day-2 to day-4, while fish in treatment-C spawned only on day-7. The reproductive performance parameters showed no significant difference in all treatments except the hatching rate parameter of treatment-B. The study concludes that better reproductive performance of black ghost knifefish, A. albifrons can be achieved with a spawning ratio of two males and three females. Further research on individual and mass spawning methods with the best spawning ratio of the fish is required.

scholarly journals Internal Quality Evolution of Open-Source Software Systems

2021 ◽  
Vol 11 (12) ◽  
pp. 5690
Author(s):  
Mamdouh Alenezi
Keyword(s):  
Open Source ◽  
Open Source Software ◽  
Software Evolution ◽  
Quality Metrics ◽  
Internal Quality ◽  
Software Systems ◽  
Software Metric ◽  
Significant Difference ◽  
The Relationship

The evolution of software is necessary for the success of software systems. Studying the evolution of software and understanding it is a vocal topic of study in software engineering. One of the primary concepts of software evolution is that the internal quality of a software system declines when it evolves. In this paper, the method of evolution of the internal quality of object-oriented open-source software systems has been examined by applying a software metric approach. More specifically, we analyze how software systems evolve over versions regarding size and the relationship between size and different internal quality metrics. The results and observations of this research include: (i) there is a significant difference between different systems concerning the LOC variable (ii) there is a significant correlation between all pairwise comparisons of internal quality metrics, and (iii) the effect of complexity and inheritance on the LOC was positive and significant, while the effect of Coupling and Cohesion was not significant.

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