scholarly journals Diversity, Chemical Constituents, and Biological Activities of Endophytic Fungi Isolated From Ligusticum chuanxiong Hort

2021 ◽  
Vol 12 ◽  
Author(s):  
Zizhong Tang ◽  
Yihan Qin ◽  
Wenhui Chen ◽  
Zhiqiao Zhao ◽  
Wenjie Lin ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Penicillium Oxalicum ◽  
Ligusticum Chuanxiong ◽  
Butanol Extract

The objective of this study was to evaluate the diversity of endophytic fungi of different parts of Ligusticum chuanxiong Hort (CX) and further characterize their biological activities and identify chemical compounds produced by these endophytic fungi. A total of 21 endophytic fungi were isolated and identified from CX. Penicillium oxalicum, Simplicillium sp., and Colletotrichum sp. were identified as promising strains by the color reaction. Comparing different organic extracts of the three strains, it was observed that the ethyl acetate extract of Penicillium oxalicum and Simplicillium sp. and the n-butanol extract of Colletotrichum sp. showed significant antioxidant and antibacterial activities. The ethyl acetate extracts of Penicillium oxalicum had outstanding antioxidant and antibacterial effects, and its radical scavenging rates for ABTS and DPPH were 98.43 ± 0.006% and 90.11 ± 0.032%, respectively. At the same time, their IC50 values were only 0.18 ± 0.02 mg/mL and 0.04 ± 0.003 mg/mL. The ethyl acetate extract of Penicillium oxalicum showed MIC value of only 0.5 mg/mL against Escherichia coli and Staphylococcus aureus. By liquid chromatography-mass spectrometry (LC-MS), we found that Penicillium oxalicum could produce many high-value polyphenols, such as hesperidin (36.06 μmol/g), ferulic acid (1.17 μmol/g), and alternariol (12.64 μmol/g), which can be a potential resource for the pharmaceutical industry. In conclusion, these results increase the diversity of CX endophytic fungi and the antioxidant and antibacterial activities of their secondary metabolites.

scholarly journals Studies on Chemical Constituents and Biological Activities of an Endophytic Fungi from Magnifera indica L.

2015 ◽  
Vol 13 (1) ◽  
pp. 63-67
Author(s):  
Mozammel Haque ◽  
Mohammad Shoeb ◽  
Nilufar Nahar
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Diffusion Method ◽  
Published Data ◽  
Nmr Studies ◽  
Brine Shrimp Lethality Assay

Two compounds, ergosterol (1) and 4-hydroxy-hexadec-6-enoic acid methyl ester (2) were isolated from the ethyl acetate extract of the endophytic fungal strain labeled as MI-3, isolated from the leave of Magnifera indica L. The structures of the isolated compounds were elucidated by 1H NMR studies and comparing with published data. The crude ethyl acetate extract, three column fractions and ergosterol were tested for antimicrobial activity against five Gram-positive and eight Gram-negative bacteria and three fungi by disc diffusion method. The general toxicity and antioxidant activity of the parent extract, column fractions and ergosterol were also evaluated by using brine shrimp lethality assay and free radical scavenging assay, respectively. Low activities were observed in all cases. DOI: http://dx.doi.org/10.3329/dujps.v13i1.21862 Dhaka Univ. J. Pharm. Sci. 13(1): 63-67, 2014 (June)

scholarly journals A Comparative Study on Antioxidation and Antibacterial Activities Triphala Herb Extracts from Chae Son, Lampang, Thailand

2020 ◽  
pp. 1-14
Author(s):  
Wilart Pompimon ◽  
Wipanoot Baison ◽  
Angkhana Chuajedton ◽  
Phansuang Udomputtimekakul ◽  
Punchavee Sombutsiri ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Antioxidant Power ◽  
Methanolic Extracts

Introduction: Triphala, a herbal medicine which is a product from Chae Son, Lampang, Thailand, is an unequal-proportional mixture of fruits of three herbs, Phyllanthus emblica, Terminalia chebula, and Terminalia bellirica (2:1:1). Materials and Methods: The contemporary study concentrated on extracts detection and comparative analysis between various annual year (A.D.2017/2018) activities in hexane, ethyl acetate, and methanolic extracts of Triphala. The determination of antioxidant was using by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP). The broth dilution and agar well diffusion assay were determined antibacterial. Results: Results exposed the valuable biological activity extracts from ethyl acetate and methanol solvents of T. chebula and T. bellirica, which strength is accountable for biological activities. Extracts displayed acceptable radical-scavenging activity analogous with ascorbic acid. The methanol extract of T. bellirica (0.0013 mg/mL), ethyl acetate extract of P. emblica (0.0009 mg/mL), in A.D. 2017 and the ethyl acetate extract of P. emblica (0.0090 mg/mL), the methanol extract of Triphala (0.0176 mg/mL) in 2018, were exhibited higher antioxidant activity. Also, extracts indicated hopeful antibacterial potential against tested strain comparable to chloramphenicol. Conclusion: The results in A.D. 2017 showed that inhibition zone of P. emblica in ethyl acetate extract; S. aureas (8.67 mm), E. aerogenes (8.67 mm), E. coli (EPEC) (7.67 mm), P. mirabilis (7.73 mm) , S. typhimurium (8.33 mm), S. flexneri (8.33 mm) and V. cholera (7.33 mm) together with P. emblica methanol extract; S. aureas (6.67 mm), E. aerogenes (7.76 mm.)  and V. cholera (4.0 mm). Therefore, it could be concluded that Triphala may be a talented candidate in pharmaceutical and future medicine.

In Vitro Screening for Cytotoxic, Anti-bacterial, Anti-HIV1-RT Activities and Chemical Constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii

2021 ◽  
Vol 11 ◽  
Author(s):  
Charina Worarat ◽  
Wilart Pompimon ◽  
Phansuang Udomputtimekakul ◽  
Sukee Sukdee ◽  
Punchavee Sombutsiri ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Bacterial Activity ◽  
Cytotoxic Activities ◽  
Separation And Purification ◽  
Crude Extracts ◽  
Cytotoxicity Activities

Background: Although the chemical constituents and biological activities of a large number of plants in the Croton genus have been studied, there are still recently discovered plants to be investigated. Objective: 1. To investigate the anti-bacterial, anti-HIV1-RT, and cytotoxicity activities of crude extracts from these plants. 2. To investigate the chemical constituents of Croton fluviatilis, Croton acutifolius, and Croton thorelii. Method: The anti-bacterial, anti-HIV1-RT, and cytotoxicity of the three plants were evaluated by standard techniques. Extraction, separation, and purification of extracts from the three plants were undertaken. Results: The ethyl acetate extract of C. fluviatilis showed low anti-bacterial activity against E. aerogenes, E. coli 0157: H7, and P. mirabilis, together with the ethyl acetate extract of C. acutifolius displayed low anti-bacterial activity against E. aerogenes, while all the crude extracts of C. thorelii were inactive. The ethyl acetate extracts of C. thorelii, and C. fluviatilis showed strong inhibited HIV1-RT, whereas the ethyl acetate extract of C. acutifolius, and the hexane extract of C. fluviatilis displayed moderate inhibited HIV1-RT. Cytotoxic properties of three Croton plants were specific to KKU-M213, MDA-MB-231, A-549, and MMNK-1. Especially, the ethyl acetate extract of C. acutifolius exhibited strong cytotoxic activities against MDA-MB-231, A-549, and MMNK-1. Furthermore, the ethyl acetate extract of C. thorelii showed high cytotoxic activities against KKU-M213, and MDA-MB-231. Compounds 1, and 4 were found in C. fluviatilis. Compounds 2 and 4 were also found in C. acutifolius. Moreover, compound 3 was only found in C. thorelii. Conclusion: The present study revealed that the three Croton species are good sources of flavonoid compounds and further investigation of the chemical constituents from these plants may prove to be fruitful to discover more active compounds to be tested as potential medicines.

scholarly journals Antibacterial Activities of Endophytic Fungi Isolated from Mentha cordifolia Leaves and Their Volatile Constituents

2016 ◽  
Vol 11 (9) ◽  
pp. 1934578X1601100
Author(s):  
Sakon Monggoot ◽  
Jariya Burawat ◽  
Patcharee Pripdeevech
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
High Concentration ◽  
Phenyl Ethyl Alcohol ◽  
First Time

A total of 17 endophytic fungal isolates were obtained from the leaves of Mentha cordifolia Opiz (Lamiaceae). Seven isolates were identified to the level of genus by using taxonomically relevant morphological traits. Colletotrichum and Phomopsis species were dominant among these strains. All strains were separated from M. cordifolia leaf for the first time. The ethyl acetate extracts of all endophytic fungi were tested for antibacterial activity against Salmonella typhimurium TISTR1166 and Pseudomonas aeruginosa TISTR781. Most endophytes exhibited antibacterial activity. Ustilago sp. MFLUCC15-1024 presented the highest inhibition zone diameter with a MIC of 31.25 μg/mL against the tested pathogens. The chemical composition of the ethyl acetate extract of this strain was investigated using gas chromatography-mass spectrometry. Twenty-one components were identified. 2-Phenylethanol (38.7%), E-ligustilide (12.4%), α-eudesmol (10.2%), β-vetivone (4.6%), β-ylangene (3.7%) and verbanol (3.4%) were the major components of the extract. The strong antibacterial activity of Ustilago sp. MFLUCC15-1024 ethyl acetate extract may be attributed to the presence of a high concentration of bioactive compounds including phenyl ethyl alcohol, E-ligustilide and α-eudesmol. The results indicate that there is high diversity of endophytic fungi in M. cordifolia leaf, and that Ustilago sp. MFLUCC15-1024 strain could be an excellent resource of natural antibacterial compounds.

scholarly journals In Vitro Pharmacological Activities and GC-MS Analysis of Different Solvent Extracts ofLantana camaraLeaves Collected from Tropical Region of Malaysia

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Mallappa Kumara Swamy ◽  
Uma Rani Sinniah ◽  
Mohd. Sayeed Akhtar
Keyword(s):  
Ethyl Acetate ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Acid Methyl Ester ◽  
Antibacterial Activities ◽  
Tropical Region ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Ms Analysis

We investigated the effect of different solvents (ethyl acetate, methanol, acetone, and chloroform) on the extraction of phytoconstituents fromLantana camaraleaves and their antioxidant and antibacterial activities. Further, GC-MS analysis was carried out to identify the bioactive chemical constituents occurring in the active extract. The results revealed the presence of various phytocompounds in the extracts. The methanol solvent recovered higher extractable compounds (14.4% of yield) and contained the highest phenolic (92.8 mg GAE/g) and flavonoid (26.5 mg RE/g) content. DPPH radical scavenging assay showed the IC50value of 165, 200, 245, and 440 μg/mL for methanol, ethyl acetate, acetone, and chloroform extracts, respectively. The hydroxyl scavenging activity test showed the IC50value of 110, 240, 300, and 510 μg/mL for methanol, ethyl acetate, acetone, and chloroform extracts, respectively. Gram negative bacterial pathogens (E. coliandK. pneumoniae) were more susceptible to all extracts compared to Gram positive bacteria (M. luteus,B. subtilis, andS. aureus). Methanol extract had the highest inhibition activity against all the tested microbes. Moreover, methanolic extract ofL. camaracontained 32 bioactive components as revealed by GC-MS study. The identified major compounds included hexadecanoic acid (5.197%), phytol (4.528%), caryophyllene oxide (4.605%), and 9,12,15-octadecatrienoic acid, methyl ester, (Z,Z,Z)- (3.751%).

scholarly journals Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes tch4 extract

2021 ◽  
pp. 17-17
Author(s):  
Janpen Tangjitjaroenkun ◽  
Wanchai Pluempanupat ◽  
Rungnapa Tangchitcharoenkhul ◽  
Waraporn Yahayo ◽  
Roongtawan Supabphol
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Bioactive Metabolites ◽  
Total Phenolic ◽  
Anticancer Activities ◽  
Antioxidant Power ◽  
Dry Extract

The in vitro biological activities of the ethyl acetate extract of the culture filtrate from Streptomyces achromogenes TCH4 (TCH4 extract) were evaluated. The ethyl acetate extract of TCH4 produced a bacteriostatic effect against Enterobacter cloacae, Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus subtilis, methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae. The extract had bactericidal activity against S. aureus, S. saprophyticus, S. aureus (MRSA) and K. pneumoniae with minimum bactericidal concentration (MBC) values in the range of 500-1000 ?g/mL. The total phenolic and flavonoid contents in TCH4 were 107.20?2.57 mg gallic acid equivalent (GAE)/g and 44.91?0.84 mg quercetin equivalent (QE)/g of dry extract. Antioxidant activity was determined by DPPH radical (IC50 299.64?6.83 ?g/mL) and ABTS radical scavenging (IC50 65.53?0.95 ?g/mL), and the ferric-reducing antioxidant power (FRAP) (822.76?9.12 mM FeSO4.7H2O/g dry extract) assays. TCH4 exhibited cytotoxic activity in the DU-145 cell line (IC50 9.36?0.37 ?g/mL). Analysis of extract constituents by GC-MS revealed pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (36.85%), benzeneacetamide (23.76%), and deferoxamine (12.85%) as the major compounds, which have been reported to possess pharmaceutical properties. S. achromogenes TCH4 could be a potential source of bioactive metabolites with antibacterial, antioxidant and anticancer activities for pharmaceutical applications.

scholarly journals Antibacterial and Antioxidant Activities of Leaf Extracts from Juglans sinensis, and its Phenolic Compositions

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201
Author(s):  
Kook-Il Han ◽  
Eui-Gil Jung ◽  
Bharat Bhusan Patnaik ◽  
Chang-Il Hong ◽  
Young-Jun Kim ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
High Performance ◽  
Inhibitory Concentration ◽  
Ethyl Acetate Extract ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Farming Practices ◽  
Leaf Extracts ◽  
Concentration Method

The beneficial properties of walnut depend on climate, genotype, and farming practices. We investigated the antibacterial and antioxidant activities of Juglans sinensis Dode cultivars grown in South Korea. Antibacterial activities were assessed using the minimum inhibitory concentration method and antioxidant activities using the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Ethyl acetate and chloroform extracts of J. sinensis leaves showed the highest antibacterial activity against Staphylococcus aureus SG511, Klebsiella aerogenes 1522E, and/or Enterobacter cloacae P99. DPPH and ABTS radical scavenging activities were found to be highest in the ethyl acetate extract. The total flavonoid and polyphenolic contents in the extract were 219.27 mg/g quercetin equivalents and 245.11 mg/g of gallic acid equivalents, respectively. Phenolic acids (gallic and vanillic) and flavonoids (catechin, rutin, and juglone) were detected by high performance liquid chromatography. We conclude that J. sinensis cultivars provide accessible sources of natural antibacterials and antioxidants.

scholarly journals Phytochemical and Biological Studies on Bark Extract of Aporosa wallichii Hook.f.

2020 ◽  
Vol 19 (2) ◽  
pp. 139-143
Author(s):  
Kazi Ruhullah Shahriar ◽  
Sirajis Salekin ◽  
Quazi Sufia Islam ◽  
Monira Ahsan ◽  
Choudhury M Hasan
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Soluble Fraction ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Bark Extract ◽  
Separation And Purification ◽  
Biological Studies

The ethyl acetate extract of bark of Aporosa wallichii Hook.f. was extensively investigated for the chemical characterization of secondary metabolites and for biological activities. After repeated chromatographic separation and purification, two compounds were elucidated as a phenolic compound ferulic acid (1) and an uncommon triterpene glut-5(6)-en-3β-ol (2) from the ethyl acetate extract of barks of A. wallichii by using high field NMR analyses. This is the first report of isolation of both compounds from this plant species. No significant antimicrobial activity was observed for any fraction after Kupchan partitioning of the extract. During the antioxidant activity assay, the ethyl acetate soluble fraction of A. wallichii demonstrated significant DPPH radical scavenging capacity with an IC50 value of 1.25 μg/mL. The other fractions viz. petroleum ether, aqueous and chloroform soluble fractions exhibited moderate, mild, and weak activity respectively. The petroleum ether soluble fraction demonstrated maximum thrombolytic property (51.33%) which was similar to that of standard streptokinase (66.81%). Dhaka Univ. J. Pharm. Sci. 19(2): 139-143, 2020 (December)

scholarly journals STUDY ON CHEMICAL CONSTITUENT AND BIOACTIVITIES OF THE FRUITS OF DIPTEROCARPUS RETUSUS BLUME. DIPTEROCARPACEAE OF VIETNAM

2019 ◽  
Vol 57 (3) ◽  
pp. 294
Author(s):  
Ho Dac Hung ◽  
Doan Huy Tien ◽  
Nguyen Thi Ngoan ◽  
Ba Thi Duong ◽  
Do Quoc Viet ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Chemical Constituent ◽  
Cytotoxic Activities ◽  
Spectroscopic Methods ◽  
Trans Stilbene

The first study on chemical constituents and biological activities of fruits of Dipterocarpus retusus Blume (Dipterocarpaceae.) growing in Vietnam was reported. Column chromatography of the ethyl acetate extract led to the isolation of five compounds: eleutherol (1), 3, 5, 4' - trihydroxy - trans - stilbene (2), polydatin (3) and β-sitosterol (4), β-sitosterol-3-O-β-D-glucopyranoside (5). Their structures were elucidated based on the NMR spectroscopic methods and comparison with literature date. The extracts from fruits of D. retusus Blume by n-hexane, ethyl acetate and butanol, consecutively, were evaluated for antioxidative and cytotoxic activities.

scholarly journals Evaluation of antioxidant, antibacterial and wound healing activities of Vitex pinnata

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 187
Author(s):  
Nurul Ashifah Shafie ◽  
Nur Atiqah Suhaili ◽  
Hussein Taha ◽  
Norhayati Ahmad
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Wound Model ◽  
Methanolic Extracts ◽  
Diffusion Assay ◽  
Dpph Radical Scavenging ◽  
Wound Healing Activity

Background: Vitex pinnata is a popular ethnomedicinal plant but scientific studies to validate its pharmacological properties are lacking for this plant. This study aims to determine the antioxidant, antimicrobial and wound healing properties of the methanolic extract of the leaves and the hexane, chloroform and ethyl acetate fractions.    Methods: The leaves of Vitex pinnata underwent methanol extraction and the methanol extract was fractionated with hexane, chloroform and ethyl acetate solvents. The antioxidant activity was determined using the DPPH radical scavenging assay. The antimicrobial activity was assessed by disc diffusion assay against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa. For the wound healing studies, the methanolic extracts of V. pinnata were used to prepare ointments with compositions of 10% (w/w) and 50% (w/w), which were evaluated for wound healing activity in an excision wound model in Wistar rats. Results: All the extracts showed antioxidant activities, with the ethyl acetate extract having the highest DPPH radical scavenging activity, followed by the methanol, chloroform and hexane extracts. Similarly, their quercetin equivalent concentrations were 33.1, 31, 20.3 and 4.5 mg/mL, respectively. Except for the methanol extract, the disc diffusion assay showed that the extracts demonstrated species-specific antibacterial activities, with the ethyl acetate extract showing antibacterial activities against all four tested strains. The wound healing activity of the high dose treated group (50% [w/w]) shows significant increase of wound contraction when compared to the control group. Conclusion: In the current study, the ethyl acetate extract showed activity for all tested bacteria and also had the highest DPPH activity. The methanolic extracts of V. pinnata leaves show modest wound healing activity in an excision wound model.

Sign in / Sign up

Export Citation Format

Share Document