Evaluation of the Bone Healing Process Utilizing Platelet-Rich Plasma Activated by Thrombin and Calcium Chloride: A Histologic Study in Rabbit Calvaria

2013 ◽  
Vol 39 (1) ◽  
pp. 14-21 ◽  
Author(s):  
Walter Betoni-Junior ◽  
Paula Dechichi ◽  
Jônatas Caldeira Esteves ◽  
Darceny Zanetta-Barbosa ◽  
Aparecido Eurípedes Onório Magalhães
Keyword(s):  
Bone Graft ◽  
Calcium Chloride ◽  
Bone Healing ◽  
Bone Repair ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Bone Defects ◽  
Histomorphometric Analysis ◽  
Histologic Study ◽  
Vascularized Bone

To evaluate the bone healing of defects filled with particulate bone graft in combination with platelet-rich plasma (PRP), added with a mixture of calcium chloride and thrombin or just calcium chloride. Two 5-mm bone defects were created in the calvaria of 24 rabbits. Each defect was filled with particulate bone graft and PRP. In one defect the PRP was activated by a mixture of calcium chloride and thrombin; in the other, PRP was activated by calcium chloride only. The animals were euthanized 1, 2, 4, and 8 weeks after the surgeries, and the calvaria was submitted to histologic processing for histomorphometric analysis. The qualitative analysis has shown that both defects presented the same histologic characteristics so that a better organized, more mature, and well-vascularized bone tissue was noticed in the eighth week. A good bone repair was achieved using either the mixture of calcium chloride and thrombin or the calcium chloride alone as a restarting agent of the coagulation process.

scholarly journals Effect of platelet-rich plasma on implant bone defects in rabbits through the FAK/PI3K/AKT signaling pathway

2019 ◽  
Vol 14 (1) ◽  
pp. 311-317
Author(s):  
Wei Liu ◽  
Ben Chen ◽  
Youyang Zheng ◽  
Yuehua Shi ◽  
Zhuojin Shi
Keyword(s):  
Growth Factor ◽  
Bone Healing ◽  
Alveolar Bone ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Bone Defects ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Tissue Healing ◽  
Bone Healing Process

AbstractPlatelet-rich plasma (PRP) has been shown to be a beneficial growth factor for bone tissue healing and is used in implantology. The aim of this study was to investigate the effects of PRP on bone defects in rabbits. Twenty rabbits were used to establish the implant bone defect model in this study. An intrabony defect (5mm × 5mm × 3mm) was created in alveolar bone in the lower jar of each rabbit. The wound was treated with PRP. The expression of platelet-derived growth factor BB (PDGFBB) was assessed by enzyme-linked immunosorbent assay (ELISA). Focal adhesion kinase (FAK) and related phosphatidylinositol 3-kinase (PI3K)/AKT (protein kinase B) levels were measured by Western blot. The results show that PRP could significantly improve the bone healing process when compared with control, and 10% PRP could markedly increase fibroblast proliferation 48-h post treatment. PDGFBB was higher in the PRP group than that in the control group. PRP treatment also could elevate the phosphorylation of FAK and PI3K/AKT, although the inhibitor of PDGFR could reverse this trend. These results suggest that PRP treatment improves the bone healing process through the FAK/PI3K/AKT pathway.

Effect of Platelet-Rich Plasma on Peri-Implant Bone Repair: A Histologic Study in Dogs

2010 ◽  
Vol 36 (4) ◽  
pp. 281-290 ◽  
Author(s):  
Ricardo V. Garcia ◽  
Marisa A. Cabrini Gabrielli ◽  
Eduardo Hochuli-Vieira ◽  
Luis C. Spolidorio ◽  
Joao G. Padilha Filho ◽  
...  
Keyword(s):  
Bone Healing ◽  
Bone Repair ◽  
Platelet Rich Plasma ◽  
Control Group ◽  
Histologic Study ◽  
Significant Difference ◽  
Bone To Implant Contact ◽  
Bone Trabeculae ◽  
Etched Surface ◽  
Enhance Bone Formation

Abstract The present study evaluated the effect of platelet-rich plasma (PRP) on peri-implant bone healing. A total of 9 mongrel dogs received 36 dental implants with sandblasted acid-etched surface in lower jaws in a split-mouth design: in the PRP group (n  =  18 implants) the implants were placed in association with PRP, and in the control group (n  =  18 implants) the implants were placed without PRP. Biopsies were obtained and prepared for histologic and histometric analysis after 15, 30, and 55 days of healing. The biopsies retrieved at 15 days showed delicate bone trabeculae formed by immature bone with presence of numerous osteoblasts for both groups. At 30 days the trabeculae presented reversal lines and evident lamellar disposition, where some thread spaces were filled by bone and dense connective tissue. At 55 days, bone healing was not altered in the control group, and histologic aspects were variable for the group treated with PRP. There was no significant difference between the groups for bone-to-implant contact (P > .05). PRP did not enhance bone formation around sandblasted acid-etched implants.

Influence of the proportion of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects

Bone ◽  
2009 ◽  
Vol 45 (2) ◽  
pp. 339-345 ◽  
Author(s):  
Maria Nagata ◽  
Michel Messora ◽  
Roberta Okamoto ◽  
Natália Campos ◽  
Natália Pola ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Healing ◽  
Critical Size ◽  
Platelet Rich Plasma ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Critical Size Defects

scholarly journals Preliminary in Vivo Evaluation of Bone Healing in Critical Size Bone Defects Implanted with an Injectable Calcium Phosphate Bone Cement (Osteopaste)

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Che Nor Zarida Che Seman ◽  
Zamzuri Zakaria ◽  
Zunariah Buyong ◽  
Mohd Shukrimi Awang ◽  
Ahmad Razali Md Ralib @ Md Raghib
Keyword(s):  
Calcium Phosphate ◽  
Bone Cement ◽  
Bone Tissue ◽  
Bone Repair ◽  
Critical Size ◽  
Healing Process ◽  
Bone Defects ◽  
Calcium Phosphate Bone Cement ◽  
Rabbit Tibia

Introduction: A novel injectable calcium phosphate bone cement (osteopaste) has been developed. Its potential application in orthopaedics as a filler of bone defects has been studied. The biomaterial was composed of tetra-calcium phosphate (TTCP) and tricalcium phosphate (TCP) powder. The aim of the present study was to evaluate the healing process of osteopaste in rabbit tibia. Materials and method: The implantation procedure was carried out on thirty-nine of New Zealand white rabbits. The in vivo bone formation was investigated by either implanting the Osteopaste, Jectos or MIIG – X3 into a critical size defect (CSD) model in the proximal tibial metaphysis. CSD without treatment served as negative control. After 1 day, 6 and 12 weeks, the rabbits were euthanized, the bone were harvested and subjected for analysis. Results: Radiological images and histological sections revealed integration of implants with bone tissue with no signs of graft rejection. There was direct contact between osteopaste material and host bone. The new bone was seen bridging the defect. Conclusion: The result showed that Osteopaste could be a new promising biomaterial for bone repair and has a potential in bone tissue engineering.

scholarly journals Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs

2020 ◽  
Vol 8 (1) ◽  
pp. 7 ◽  
Author(s):  
Savvas Titsinides ◽  
Theodore Karatzas ◽  
Despoina Perrea ◽  
Efstathios Eleftheriadis ◽  
Leonidas Podaropoulos ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Healing Process ◽  
Bone Substitutes ◽  
Bone Defects ◽  
New Bone Formation ◽  
Post Surgery ◽  
Beta Tricalcium Phosphate ◽  
Close Proximity ◽  
Tissue Specimens

Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine xenograft. Materials were placed in 3 out of the 4 bone cavities, while 1 deficit was left empty, serving as a control, in mono-cortical defects, surgically prepared in the porcine calvaria bone. Animals were randomized in 2 groups and euthanized at 8 and 12 weeks. Harvested tissue specimens were qualitatively evaluated by histology. New bone formation was quantitatively measured by histomorphometry. Maximum new bone formation was noticed in defects grafted with beta-tricalcium phosphate b-TCP compared to the other bone substitutes, at 8 and 12 weeks post-surgery. Bovine and human allograft induced less new bone formation compared to empty bone cavity. Histologic analysis revealed that b-TCP was absorbed and substituted significantly, while bovine and human allograft was maintained almost intact in close proximity with new bone. Based on our findings, higher new bone formation was detected in defects filled with b-TCP when compared to bovine and human graft substitutes.

scholarly journals Morphology of autogenous bone graft and castor oil polyurethane in the infraorbital rim of rabbits: a comparative study

2006 ◽  
Vol 21 (5) ◽  
pp. 341-347 ◽  
Author(s):  
José Carlos Garcia de Mendonça ◽  
Rafael De Rossi ◽  
Celso Massaschi Inouye ◽  
Diego Rodrigo Paulillo Bazan ◽  
João Carlos Castro Monteiro ◽  
...  
Keyword(s):  
Bone Graft ◽  
Castor Oil ◽  
Bone Repair ◽  
Bone Defects ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Control Group ◽  
Cell Nuclei ◽  
Zygomatic Bone ◽  
Chi Squared

PURPOSE: Morphological study comparing castor oil polyurethane and autogenous bone graft to repair bone defect in zygomatic bone of rabbits. METHODS: Twenty-four adult, male New Zealand rabbits were randomly distributed between two groups of twelve. Bone defects of 5mm in diameter were cut through the zygomatic bone and filled with polyurethane discs in the experimental group or autogenous bone harvested from the tibia in the control group. Animals were sacrificed after 30, 60 or 90 days, and the zygomatic bones were macro- and microscopically analyzed. Student's, Fisher's, chi-squared and McNemar's tests were used for statistical analysis. RESULTS: Both the castor oil polyurethane and the autograft adapted well to the defect, with no need for fixation. Fibrous connective tissue encapsulated the polyurethane, but no inflammation or giant cell reaction was observed. Acidophilic and basophilic areas were observed inside the micropores of the polyurethane, suggesting cell nuclei. After 90 days, bone repair with a lamellar pattern of organization was observed in the control group. CONCLUSION: The castor oil polyurethane was biocompatible and did not cause inflammation. It may be considered an alternative to fill bone defects.

LOCAL APPLICATION OF GROWTH HORMONE INDUCES INCREASED IMMUNOEXPRESSION OF IGF-I ON CRANIOFACIAL BONE DEFECTS REPAIR – A PILOT STUDY

2021 ◽  
pp. 31-35
Author(s):  
Felipe Rychuv Santos ◽  
Carmen L. Mueller Storrer ◽  
Suyany Gabriely Weiss ◽  
Leandro Kluppel ◽  
João César Zielak ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Bone Repair ◽  
Healing Process ◽  
Bone Defects ◽  
Local Effect ◽  
Control Group ◽  
Craniofacial Bone ◽  
Factor I ◽  
Collagen Group ◽  
Igf I

The aim is to evaluate the local effect of different concentrations of growth hormone (GH) on the repair of craniofacial bone defects, through histological, histomorfometric, and insulin-like growth factor I (IGF-I) immunoexpression assessments. Critical defects (5 mm) were performed in 32 Wistar rats. The animals were divided into four groups: Group C (Control); Group S (Sponge-collagen); Group GH 0.08 mL; GH 0.104 mg; Group GH 0.1mL. Local applications were performed 3 times a week until the rats were euthanized at 60 days. The data were submitted to ANOVA and Tukey's test (P < 0.05). A healing process with predominance of collagen bers and bone neoformation near the edges of the defect was observed in groups C and S. Islands of bone neoformation were observed at the center and edges of the defect in groups GH 0.08 and GH 0.1. In GH 0.1, the bone was more compact, and the defect was completely closed in some specimens. Bone neoformation was signicantly higher in the GH-treated groups. All the specimens stained positive for IGF-I, and this immunoexpression was signicantly higher in Group GH 0.1. In conclusion, locally applied GH signicantly favored bone repair in rat calvaria, and a higher dose of GH increased the immunoexpression of IGF-I.

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